Ischemic stroke （IS） is a serious cerebrovascular disease with high clinical incidence. Cerebral ischemia and hypoxia can induce metabolic reprogramming of cellular glycolysis. Hypoxia-inducible factor-1α （HIF-1α） serves as the core transcription factor in the hypoxic response. This article systematically reviews the molecular mechanisms of HIF-1α-mediated glycolytic reprogramming， elaborates on the effects of this metabolic alteration on the functional status of different immune cells， and summarizes the research progress of traditional Chinese medicine （TCM） interventions in this pathological process. HIF-1α reshapes glucose metabolic flux and shifts cellular metabolism toward glycolysis by regulating the transcription of key genes， including glucose transporters， glycolytic rate-limiting enzymes， and mitochondrial oxidative inhibitors. This metabolic disturbance can regulate the activation status， phenotypic differentiation， and functional remodeling of microglia， astrocytes， and peripherally infiltrated immune cells， thereby participating in the mediation of neuroinflammatory responses after IS. Moreover， multiple signaling pathways and the metabolite of glycolysis lactate can exert feedback regulation on this metabolic-immune process. Various active components of TCM and herbal formulas， such as astragaloside Ⅳ， salvianolic acid C， Ginkgo biloba extract， and Mailuo shutong pills， can inhibit abnormal glycolysis， reduce lactate accumulation， and restore the homeostasis of glucose oxidative metabolism by regulating signaling pathways including AMP-activated protein kinase/mammalian target of rapamycin/HIF-1α， HIF-1α/pyruvate kinase M2， and HIF-1α/lactate dehydrogenase A. Consequently， they effectively alleviate neuroimmune inflammation and attenuate ischemic brain injury.

Objective:To investigate the expression of circular RNA hsa_circ_0046701 in glioma tissues and its effect on the proliferation,migration and invasion of glioma U251 cells.Methods:Tumor tissue specimens and clinical data of fifty-two glioma patients treated surgically in Putuo People's Hospital Affiliated to Tongji University between June 2022 and March 2023 were collected,and another 30 normal brain tissue specimens were collected as controls.The expression level of hsa_circ_0046701 in glioma tissues was detected by qPCR to analyze the relationship between the level and clinical characteristics of the patient.Kaplan-Meier method was used to analyze the relationship between hsa_circ_0046701 level and survival prognosis.Using RNA interference technology,circ_0046701 overexpression and empty vector,siRNA-circ_0046701 and negative control(si-NC)plasmid were transfected into glioma U251 cells,respectively.The experiment cells were divided into the circ 0046701 OE group,Vector group,si-circ_0046701 group and si-NC group.CCK-8 method and Transwell assay were applied to detect the proliferation,migration and invasion abilities of cells in each group.Western blotting was applied to detect the expressions of vimentin,Snail,E-cadherin and cyclin D1 proteins in cells of each group.Results:The expression level of hsa_circ_0046701 in glioma tissues was significantly higher than that in normal brain tissues(P<0.01).The percentage of patients with WHO glioma grading(grades Ⅲ～Ⅳ)in the hsa_circ_0046701 high-expression group was significantly higher than that of the hsa_circ_0046701 low expression group(P<0.01).The OS of patients in the high-expression group was significantly shorter than that of the low-expression group.Compared with those in the si-NC group,the proliferation ability of U251 cells in the si-circ_0046701 group was significantly reduced(P<0.05 or P<0.01),and the numbers of migrated and invaded cells decreased significantly(both P<0.01).The expressions of vimentin,Snail,and cyclin D1 proteins in the cells was significantly reduced(all P<0.01),while the expression of E-cadherin protein increased significantly(P<0.01).Compared with those in the Vector group,the proliferation ability of U251cells in the circ_0046701 OE group increased significantly(P<0.01);the numbers of migrated and invaded cells increased significantly(both P<0.01);the expressions of vimentin,Snail,and cyclin D1 proteins in the cells increaseds significantly(all P<0.01),and the expression of E-cadherin protein decreased significantly(P<0.01).Conclusion:Circular RNA hsa_circ_0046701 is highly expressed in glioma tissues,and is correlated with poor patient prognosis.Knockdown of hsa_circ_0046701 expression could inhibit the proliferation,migration and invasion of glioma U251cells.

ObjectiveTo investigate the mediating role of loneliness and social anxiety in the relationship between basic psychological needs satisfaction and smartphone addiction among high school students， and to provide references for the intervention to smartphone addiction for high school students. MethodsIn April 2022， a total of 14 666 high school students from 5 middle schools in a county of Sichuan Province were included by random sampling method. Basic Psychological Needs Scales （BPNS）， the UCLA Loneliness Scale-3 edition （UCLA-3）， Interaction Anxiousness Scale （IAS） and the Mobile Phone Addiction Index （MPAI） were used for cross-sectional investigation. Bootstrap method was used to analyze the mediating role of loneliness and social anxiety in the relationship between the basic psychological needs satisfaction and smartphone addiction. Results① In this study， 14 036 valid questionnaires were returned， and 1 752 （12.48%， 95% CI： 0.114~0.136） high school students were found to be addicted to smartphone phone. The BPNS score was negatively correlated with UCLA-3， IAS and MPAI scores （r=-0.771， -0.486， -0.417， P<0.01）. And MPAI score was positively correlated with IAS and UCLA-3 scores （r=0.403， 0.424， P<0.01）. IAS score was positively correlated with UCLA-3 score （r=0.458， P<0.01）. ②The degree of basic psychological needs satisfaction in high school students can directly negatively predict smartphone addiction （β=-0.383， P<0.05）， and can also indirectly affect smartphone addiction through loneliness and social anxiety （β=-0.130， P<0.05）. ③Loneliness （indirect effect value was -0.145） and social anxiety （indirect effect value was -0.074） partially mediate between the basic psychological needs satisfaction and smartphone addiction. Loneliness-social anxiety also has significant chain mediating effect （indirect effect value was -0.034）， which accounted for 8.88%. ConclusionThe basic psychological needs satisfaction can not only directly affect the occurrence of smartphone addiction， but also indirectly affect the occurrence of smartphone addiction through the chain mediation of loneliness and social anxiety.

Objective: To explore the correlation between BMI and gut microbiota of college students in Inner Mongolia,and to provide a reference basis for revealing the relationship between intestinal flora and obesity. Methods: Totally 88 college students from Inner Mongolia Medical University were enrolled, Height and weight were measured,and the feces samples were collected. The bacterial metagenome was extracted from dry feces samples for the concentration detection in per gram of dry feces,expressed as μg/μL. Correlation between BMI and metagenomics concentration of gut microbiota was statistically analyzed. Meanwhile,the metagenomics concentration of gut microbiota in different BMI groups was compared with each other. Results: There was a negative correlation between BMI and the metagenomics concentration of gut microbiota(r=-0.27,P<0.05). Significant difference in the concentration of gut microflora was observed between the normal group and the obesity group,the normal group and the overweight/obesity group(F=3.62,P<0.05). Among the female volunteers,there were significant differences between normal group and overweight group,between normal group and obesity group(F=1.87,P<0.05). No significant differences in metagenomics concentration of gut microbiota were found in different BMI groups(F=0.60, P>0.05). Conclusion There is a correlation between BMI and gut microbiota of college students in Inner Mongolia，the concentration of gut microflora metagenome in overweight and obese people decreased significantly.

Objective To study the feasibility and efficacy of intraoperative choledoscopy combined with laparoscopic left liver resection to treat intrahepatic lithiasis.Method In 49 patients with biliary stone,laparoscopic left lateral sectionectomy/left medial sectionectomy/left hepatectomy combined with intraoperative choledochoscopy and stone retrieval were carried out.Results Laparoscopic left lateral sectionectomy was carried out in 29 patients,left medial sectionectomy in 2 patients and left hepatectomy in 18 patients.In all the patients,combined choledoscopy and stone retrieval were carried out.A concomitant laparoscopic cholecystectomy (LC) was carried out in 11 patients.The average operative time was 226 min.When LC was carried out,the mean operative time was 243 min.The mean intraoperative blood loss was 378 ml.There was no bile leak or postoperative bleeding.Flatus was passed 1-3 days after surgery.The patients were discharged home 7-10 days after surgery.Postoperative MRI/MRCP did not reveal any residual stone.On a mean follow up of 16 months for 47 patients,the patients did well and there was no recurrent stone.Conclusion Choledochoscopy combined with laparoscopic left liver resection for bile duct stone resulted in minimal trauma to the patient.The recovery was quick and there was a high stone clearance rate.The treatment was safe,efficacious,and it is a viable minimally invasive treatment option.