Objective To explore the role of CLEARstent Live(CSL),a real-time stent visualiza-tion tool,in guiding percutaneous coronary intervention(PCI)for patients with complex coronary artery disease(CAD)and its effect on the risk of restenosis of stented coronary arteries.Methods A total of 140 patients with complex CAD undergoing PCI in our hospital from September 2022 to October 2023 were enrolled,and randomly divided into routine group(conventional stent doc-king technique)and CSL group(PCI under CSL visualization),with 70 patients in each group.The quality of angiographic images,basic conditions of surgical treatment,cardiac function and inci-dence of in-stent restenosis were compared between the two groups.Results The effective radia-tion dose,developing time,and dose area product were significantly lower,and the image quality score was obviously higher in the CSL group than the conventional group(P＜0.01).There were no significant differences in success rate of stent implantation,operation time,stent length or stent number between the two groups(P＞0.05).The CSL group had notably shorter length of hospital stay(9.83±1.21 d vs 11.85±1.68 d,P=0.000)and higher treatment cost[(3.01±0.63)× 104 Yuan vs(2.79±0.52)×104 Yuan,P=0.026]when compared with the conventional group.In both group,surgical treatment resulted in significantly elevated cardiac index and decreased left ventricular end-diastolic diameter and left ventricular end-systolic diameter at 7 d after operation(P＜0.05).The incidence of in-stent restenosis was statistically lower in the CSL group than con-ventional group[4.29％(3 cases)vs 25.71％(18 cases),x2=12.605,P=0.000].Conclusion CSL visualization provides better image quality for patients with complex CAD undergoing PCI,thus reducing the risk of in-stent restenosis and improving the treatment efficacy.

Objective To explore the role of CLEARstent Live(CSL),a real-time stent visualiza-tion tool,in guiding percutaneous coronary intervention(PCI)for patients with complex coronary artery disease(CAD)and its effect on the risk of restenosis of stented coronary arteries.Methods A total of 140 patients with complex CAD undergoing PCI in our hospital from September 2022 to October 2023 were enrolled,and randomly divided into routine group(conventional stent doc-king technique)and CSL group(PCI under CSL visualization),with 70 patients in each group.The quality of angiographic images,basic conditions of surgical treatment,cardiac function and inci-dence of in-stent restenosis were compared between the two groups.Results The effective radia-tion dose,developing time,and dose area product were significantly lower,and the image quality score was obviously higher in the CSL group than the conventional group(P＜0.01).There were no significant differences in success rate of stent implantation,operation time,stent length or stent number between the two groups(P＞0.05).The CSL group had notably shorter length of hospital stay(9.83±1.21 d vs 11.85±1.68 d,P=0.000)and higher treatment cost[(3.01±0.63)× 104 Yuan vs(2.79±0.52)×104 Yuan,P=0.026]when compared with the conventional group.In both group,surgical treatment resulted in significantly elevated cardiac index and decreased left ventricular end-diastolic diameter and left ventricular end-systolic diameter at 7 d after operation(P＜0.05).The incidence of in-stent restenosis was statistically lower in the CSL group than con-ventional group[4.29％(3 cases)vs 25.71％(18 cases),x2=12.605,P=0.000].Conclusion CSL visualization provides better image quality for patients with complex CAD undergoing PCI,thus reducing the risk of in-stent restenosis and improving the treatment efficacy.

P450 fatty acid decarboxylase OleT from Staphylococcus aureus (OleTsA) is a novel cytochrome P450 enzyme that catalyzes the oxidative decarboxylation of fatty acids to yield primarily terminal alkenes and CO2 or minor α-and β-hydroxylated fatty acids as side-products.In this work,the interactions between a series of cycloalkyl phosphorus heterocycles (CPHs) and OleTsA were investigated in detail by fluores-cence titration experiment,ultraviolet-visible (UV-vis) and 31p NMR spectroscopies.Fluorescence titration experiment results clearly showed that a dynamic quenching occurred when CPH-6,a repre-sentative CPHs,interacted with OleTsA with a binding constant value of 15.2 × 104 M-1 at 293 K.The thermodynamic parameters (△H,△S and △G) showed that the hydrogen bond and van der Waals force played major roles in the interaction between OleTsA and CPHs.The UV-vis and 31p NMR studies indicated the penetration of CPH-6 into the interior environment of OleTsA,which greatly affects the enzymatic activity of OleTsA.Therefore,our study revealed an effective way to use phosphorus hetero-cyclic compounds to modulate the activity of cvtochrome P450 enzymes.