With the proposal and implementation of the Human Microbiome Project,the role of microbial communities in the occurrence and development of diseases has been increasingly valued.Sequencing technolo-gy has been widely applied in related research and has made great progress.However,there are also shortcom-ings such as high detection thresholds and inability to obtain viable bacteria.As an emerging technology,cul-turomics aims to isolate and cultivate as many different bacterial communities as possible by simulating the survival environments of bacteria and employing various culture conditions,and accurately identify the corre-sponding bacteria.This technique is widely applied in the study of microbial community characteristics across different diseases.This paper reviews the application of culturomics in disease research and explores its prom-ising integration with other omics technologies in microbiome research.This technology is widely used for studying the microbial community characteristics of different diseases.This article reviews the application of culturomics in various disease research and explores its promising prospects in microbial community research when combined with other omics techniques.