Objective To investigate the relationship between preoperative serum levels of long noncoding RNA(lncRNA)X inactive specific transcript(XIST)and microRNA(miR)-140-3p in acute myocardial infarction(AMI)patients undergoing percutaneous coronary intervention(PCI)and blood glucose,blood lipids,myocardial injury indexes,degree of coronary artery disease and the occurrence of major adverse cardiovascular events(MACE)within 3 years after surgery.Methods A total of 164 patients with AMI(AMI group)and 148 healthy subjects(healthy group)were selected from Hankuang General Hospital of North China Medical and Health Group from November 2018 to February 2021.Clinical data were collected at admission,and blood glucose,blood lipids,myocardial injury indicators,serum lncRNA XIST and miR-140-3p levels in AMI group before PCI and healthy group during physical examination were detected.According to the degree of coronary artery disease,AMI patients were divided into mild coronary artery disease group(n=33),moderate coronary artery disease group(n=76),and severe coronary artery disease group(n=55).Follow up for 3 years after surgery,AMI patients were divided into MACE group(n=36)and non-MACE group(n=128).Clinical data,preoperative PCI blood glucose,blood lipids,myocardial injury indicators,synergy between percutaneous coronary intervention with TAXUS and cardiac surgery(SYNTAX)score,and serum lncRNA XIST and miR-140-3p expression levels of health group and AMI group,AMI patients with different prognoses within 3 years after PCI were compared.The serum lncRNA XIST and miR-140-3p expression levels of AMI patients with different degrees of coronary artery disease before PCI were compared.The correlation between preoperative PCI serum lncRNA XIST and miR-140-3p with blood glucose,blood lipids,myocardial injury indicators,SYNTAX score in AMI patients,the influencing factors of MACE occurrence,and predicting the value of preoperative PCI serum lncRNA XIST and miR-140-3p in predicting MACE occurrence within 3 years after PCI in AMI patients were analyzed.Results Compared with healthy group,the preoperative PCI fasting plasma glucose(FPG),total cholesterol(TC),triglycerides(TG),low density lipoprotein-cholesterol(LDL-C),hypersensitive C-reactive protein(hs-CRP),creatine kinase isoenzyme(CK-MB),homocysteine(Hcy),cardiac troponin I(cTnI),brain natriuretic peptide(BNP),lncRNA XIST expression levels of AMI group were significantly increased(t=2.426～80.642),and high density lipoprotein-cholesterol(HDL-C),left ventricular ejection fraction(LVEF)and miR-140-3pexpression levels were significantly reduced(t=6.166～66.119),the differences were statistically significant(all P<0.05).Before PCI,the expression levels of serum lncRNA XIST were increased successively,and the expression levels of miR-140-3p were decreased successively in mild,moderate,and severe coronary artery lesions group,the differences were statistically significant(F=174.600,231.138,all P<0.05).The serum lncRNA XIST of AMI patients before PCI was positively correlated with FPG,TC,TG,LDL-C,hs-CRP,CK-MB,Hcy,cTnI,BNP and SYNTAX scores(r=0.235～0.681,all P＜0.05),and negatively correlated with miR-140-3p,HDL-C and LVEF(r=-0.571,-0.262,-0.513,all P＜0.05).miR-140-3p was negatively correlated with FPG,TC,TG,LDL-C,hs-CRP,CK-MB,Hcy,cTnI,BNP and SYNTAX scores(r=-0.672～-0.209,all P＜0.05),and positively correlated with HDL-C and LVEF(r=0.245,0.524,all P＜0.05).Compared with non-MACE group,the hs-CRP,CK-MB,Hcy,cTnI,BNP,SYNTAX scores and lncRNA XIST expression levels before PCI in MACE group were significantly increased(t=3.032～26.330),while the LVEF and miR-140-3p expression levels were significantly reduced(t=2.821,13.318),the differences were statistically significant(all P<0.05).LVEF and miR-140-3p were independent protective factors for MACE in AMI patients within 3 years after PCI(Wald χ2=6.098,7.851,all P＜0.05),while CK-MB,Hcy,cTnI,BNP,SYNTAX score and lncRNA XIST were independent risk factors for MACE in AMI patients within 3 years after PCI(Wald χ2=4.263～7.853,all P＜0.05).Compared with the area under curve(AUC)of serum lncRNA XIST and miR-140-3p before PCI separately predicting MACE occurrence within 3 years after PCI in AMI patients,the combined prediction of MACE occurrence within 3 years after PCI in AMI patients was higher,and the differences were statistically significant(Z=2.059,2.017,all P<0.05).Conclusion The expression of lncRNA XIST in the serum of AMI patients is increased while the expression of miR-140-3p is decreased.Both are associated with blood glucose,blood lipids myocardial injury indicators,degree of coronary artery disease,and the occurrence of MACE within three years after PCI.They have high value in predicting the occurrence of MACE within three years after PCI surgery.

Objective To investigate the relationship between preoperative serum levels of long noncoding RNA(lncRNA)X inactive specific transcript(XIST)and microRNA(miR)-140-3p in acute myocardial infarction(AMI)patients undergoing percutaneous coronary intervention(PCI)and blood glucose,blood lipids,myocardial injury indexes,degree of coronary artery disease and the occurrence of major adverse cardiovascular events(MACE)within 3 years after surgery.Methods A total of 164 patients with AMI(AMI group)and 148 healthy subjects(healthy group)were selected from Hankuang General Hospital of North China Medical and Health Group from November 2018 to February 2021.Clinical data were collected at admission,and blood glucose,blood lipids,myocardial injury indicators,serum lncRNA XIST and miR-140-3p levels in AMI group before PCI and healthy group during physical examination were detected.According to the degree of coronary artery disease,AMI patients were divided into mild coronary artery disease group(n=33),moderate coronary artery disease group(n=76),and severe coronary artery disease group(n=55).Follow up for 3 years after surgery,AMI patients were divided into MACE group(n=36)and non-MACE group(n=128).Clinical data,preoperative PCI blood glucose,blood lipids,myocardial injury indicators,synergy between percutaneous coronary intervention with TAXUS and cardiac surgery(SYNTAX)score,and serum lncRNA XIST and miR-140-3p expression levels of health group and AMI group,AMI patients with different prognoses within 3 years after PCI were compared.The serum lncRNA XIST and miR-140-3p expression levels of AMI patients with different degrees of coronary artery disease before PCI were compared.The correlation between preoperative PCI serum lncRNA XIST and miR-140-3p with blood glucose,blood lipids,myocardial injury indicators,SYNTAX score in AMI patients,the influencing factors of MACE occurrence,and predicting the value of preoperative PCI serum lncRNA XIST and miR-140-3p in predicting MACE occurrence within 3 years after PCI in AMI patients were analyzed.Results Compared with healthy group,the preoperative PCI fasting plasma glucose(FPG),total cholesterol(TC),triglycerides(TG),low density lipoprotein-cholesterol(LDL-C),hypersensitive C-reactive protein(hs-CRP),creatine kinase isoenzyme(CK-MB),homocysteine(Hcy),cardiac troponin I(cTnI),brain natriuretic peptide(BNP),lncRNA XIST expression levels of AMI group were significantly increased(t=2.426～80.642),and high density lipoprotein-cholesterol(HDL-C),left ventricular ejection fraction(LVEF)and miR-140-3pexpression levels were significantly reduced(t=6.166～66.119),the differences were statistically significant(all P<0.05).Before PCI,the expression levels of serum lncRNA XIST were increased successively,and the expression levels of miR-140-3p were decreased successively in mild,moderate,and severe coronary artery lesions group,the differences were statistically significant(F=174.600,231.138,all P<0.05).The serum lncRNA XIST of AMI patients before PCI was positively correlated with FPG,TC,TG,LDL-C,hs-CRP,CK-MB,Hcy,cTnI,BNP and SYNTAX scores(r=0.235～0.681,all P＜0.05),and negatively correlated with miR-140-3p,HDL-C and LVEF(r=-0.571,-0.262,-0.513,all P＜0.05).miR-140-3p was negatively correlated with FPG,TC,TG,LDL-C,hs-CRP,CK-MB,Hcy,cTnI,BNP and SYNTAX scores(r=-0.672～-0.209,all P＜0.05),and positively correlated with HDL-C and LVEF(r=0.245,0.524,all P＜0.05).Compared with non-MACE group,the hs-CRP,CK-MB,Hcy,cTnI,BNP,SYNTAX scores and lncRNA XIST expression levels before PCI in MACE group were significantly increased(t=3.032～26.330),while the LVEF and miR-140-3p expression levels were significantly reduced(t=2.821,13.318),the differences were statistically significant(all P<0.05).LVEF and miR-140-3p were independent protective factors for MACE in AMI patients within 3 years after PCI(Wald χ2=6.098,7.851,all P＜0.05),while CK-MB,Hcy,cTnI,BNP,SYNTAX score and lncRNA XIST were independent risk factors for MACE in AMI patients within 3 years after PCI(Wald χ2=4.263～7.853,all P＜0.05).Compared with the area under curve(AUC)of serum lncRNA XIST and miR-140-3p before PCI separately predicting MACE occurrence within 3 years after PCI in AMI patients,the combined prediction of MACE occurrence within 3 years after PCI in AMI patients was higher,and the differences were statistically significant(Z=2.059,2.017,all P<0.05).Conclusion The expression of lncRNA XIST in the serum of AMI patients is increased while the expression of miR-140-3p is decreased.Both are associated with blood glucose,blood lipids myocardial injury indicators,degree of coronary artery disease,and the occurrence of MACE within three years after PCI.They have high value in predicting the occurrence of MACE within three years after PCI surgery.

Objective To compare the contents of total flavonoids, forsythoside A and phillyrin in Forsythiae Fructus leaves tea under different drying conditions; To determine the best drying method for Forsythiae Fructus leaves tea. Methods With the sodium nitrite-aluminum trichloride-sodium hydroxide solution as color reagent, total flavonoids in forsythiae Fructus leaves tea were determined by UV spectrophotometry at the wavelength of 500 nm. HPLC was used to determine the contents of forsythiaside A and phillyrin. The analysis was performed on a Diamosil C18 (2) column (4.6 mm × 250 mm, 5 μm); the mobile phase was composed of acetonitrile and 0.4% acetic acid with gradient elution; the detection wavelength was set at 277 nm; the flow rate was 1.0 mL/min at column temperature of 30 ℃. Results The content of total flavonoids of Forsythiae Fructus leaves tea under different drying conditions was basically the same; the sequence of the contents of forsythoside A and Forsythin of Forsythiae Fructus leaves tea under different drying conditions was: ventilated drying > vacuum drying > blast drying. Conclusion Different drying conditions have no effect on the contents of total flavonoids in Forsythiae Fructus leaves tea, but have obvious effects on the contents of forsythiaside A and phillyrin. Ventilation shade is better than blast drying and vacuum drying for preverence of forsythiaside A and forsythin.

ObjectiveTo establish an HPLC analytic method for amygdalin inKuxingrenFormula Granules and HPLC characteristic chromatogram.MethodsRP-HPLC method was adopted. The determination was performed on Dionex C18 column (4.6 mm×250 mm, 5μm) with acetonitrile-0.1% phosphoric acid solution (8:92) at the flow rate of 0.6 mL/min. The detection wavelength was set at 207 nm and sample size was 10μL. The column temperature was 30℃. The peak of amygdalin was set as refernce, and 10 batches of samples were analyzed. TCM Chromatogram Fingerprint Similarity Evaluation System (2004 A) was adopted to evaluate similatity.Results The linear equation of amygdalin wasY=6.176×10-7X?3.058×10-3, with a good linear relationship in the range of 0.122 5–1.225μg (r= 0.999 8). The average recovery rate was 98.75% (RSD=1.30%). There were 6 common peaks in the characteristic chromatogram ofKuxingrenFormula Granules, and the similarity of 10 batches of samples was higher than 0.981. ConclusionThe HPLC method is simple, accurate and reproducible, which can be used for the quality control of Kuxingren Granules.

Objective To analyze effects of thoracoscopy in the diagnosis and treatment of suspected diaphragmatic injury after thoracoabdominal stab wound.Methods Sixty-eight patients who received thoracoscopic diagnosis and management of diaphragmatic injuries due to thoracoabdominal stab wounds from April 2000 to October 2011 were retrospectively analyzed.Results Occult diaphragmatic injuries were found in 11 patients.Seven patients underwent thoracoscopic suture,of which five had synchronous laparotomy for inspected abdominal organ injuries.Pulmonary parenchymal lacerations occurred in 15 patients who received thoracoscopic repair or resection.Coagulated hemothorax in 13 patients were removed.Postoperative complications included pleural effusion in one patient,pneumonia in two and pulmonary atelectasis in one.Hospital stay was(7.9±13.5)days,without ICU stay.The length of drainage,operation time and intraoperative blood loss were(3.3±1.5)days,(45.6±78.1)minutes and(57.8±24.3)ml respectively.There was no conversion to thoracotomy.Thoracic CT scan was performed six months postoperatively,without hernias.The accuracy of thoracoscopy in diagnosing diaphragmatic injury was 100％.Conclusion Thoracoscopy should be performed for the thoracoabdominal stab wounds with stable hemodynamics,with definite significance especially for the diagnosis and treatment of wounds at the 7-9th intercostal spaces.