Objective To establish a multiplex assay method for the simultaneous detection of FluA and FluB virus(IBV)antigen based on the flow cytometry(FCM)quantum dot-encoded bead technologies,laying the foundation for the assay of multiple respiratory virus biomarkers.Methods Coupling was performed for FluA and FluB nucleoprotein(NP)monoclonal antibodies using self-made quantum dot-encoded beads,separately.FCM was used to detect known concentrations of FluA and FluB antigens separately and simultaneously,optimize the detection conditions,and establish a joint detection method for FluA and FluB antigens.Compared with the quantitative real-time PCR(qPCR)method,clinical samples were used to evaluate the clinical performance of this joint detection method.Results The joint detection method for FluA and FluB antigens was established,with detection limits of 26.1 pg/ml and 10.7 pg/ml,respectively,and measurement ranges of 15.3～250 000 pg/ml.The joint detection method for clinical sample evaluation was well correlated with the qPCR,with a positive coincidence rate of 57.4%,a negative coincidence rate of 100%,and a total coincidence rate of 71.6%.In addition,the joint detection method was superior to colloidal gold immunochromatographic strip assay commonly used in clinical practice(positive coincidence rate of 56.49%,negative coincidence rate of 99.75%).Conclusion The FCM quantum dot-encoded bead multiplex assay can be used for the joint detection of FluA and FluB antigens,which have a high sensitivity,good specificity and wide detection range.It may lay a good foundation for the multiplex detection of common respiratory viruses,and has clinical application prospects.

Objective To investigate the relationship between procalcitonin (PCT),neutrophil to lymphocyte ratio (NLR) and prognosis of acute pancreatitis.Methods Retrospective analysis was made on the levels of PCT and NLR in 102 patients with acute pancreatitis and the same number of healthy controls who sought routine medical care in the hospital,and comparisons were made between the 2 groups.Then,the relationship between PCT/NLR levels and clinical features of the patients were analyzed.Multivariable logistic regression model and receiver operating characteristic (ROC) were used to evaluate the relationship between NLR/PCT and prognosis of acute pancreatitis.Results The levels of NLR and PCT in the patients with acute pancreatitis were significantly higher than those of the healthy individuals,and both NLR and PCT were closely associated with the patient conditions.The area under curve (AUC) for PCT and NLR were respectively 0.81 and 0.84,and the AUC in multivariable combination usage was 0.96.Conclusion Both NLR and PCT were associated with prognosis of acute pancreatitis,and the combined use of NLR and PCT would facilitate to predict the prognosis of acute pancreatitis.