Objective To investigate the relationship between serum matrix metalloproteinase-9(MMP-9),cytokeratin 19 fragment(CYRFA21-1)and vascular endothelial growth factor(VEGF)levels and cisplatin treatment in patients with non-small cell lung cancer.Methods 130 advanced B-stage non-small cell lung cancer treated with cisplatin in Affiliated Hospital of Nantong University from December 2020 to December 2022 were selected as the study subjects;100 patients with physical examination in our hospital were selected as the control group.By comparing the serum levels of MMP-9,CYRFA21-1 and VE GF in each group,the cisplatin treatment effect was divided into 15 patients and 37 patients in the treatment-effective group,and 78 patients in the treatment-effective group to clarify the relationship between the treatment effect of patients with non-small-cell lung cancer.Results Serum levels of MMP-9,CYRFA21-1 and VEGF were decreased in NSCLC patients after cisplatin treatment(P＜0.05).Compared with the ineffective group,serum MMP-9,CYRFA21-1 and VEGF levels in the significantly effective group and the effective group were decreased(P＜0.05).Logistic regression analysis showed that MMP-9,CYRFA21-1 and VEGF were also risk factors affecting cisplatin treatment effect in NSCLC patients(P＜0.05).ROC analysis showed that serum MMP-9,CYRFA21-1 and VEGF had high predictive value for cisplatin treatment in NSCLC patients(P＜0.05).Conclusions MMP-9,CYRFA21-1 and VEGF are highly expressed in the serum of patients with severe pneumonia,and the expression levels of MMP-9,CYRFA21-1 and VEGF are decreased after cisplatin treatment in patients with non-small cell lung cancer,which is related to the cisplatin treatment effect in patients with non-small cell lung cancer.Clinical monitoring of serum levels of MMP-9,CYRFA21-1 and VEGF can predict the therapeutic effect early to improve the prognosis of patients.

Objective To investigate the relationship between serum matrix metalloproteinase-9(MMP-9),cytokeratin 19 fragment(CYRFA21-1)and vascular endothelial growth factor(VEGF)levels and cisplatin treatment in patients with non-small cell lung cancer.Methods 130 advanced B-stage non-small cell lung cancer treated with cisplatin in Affiliated Hospital of Nantong University from December 2020 to December 2022 were selected as the study subjects;100 patients with physical examination in our hospital were selected as the control group.By comparing the serum levels of MMP-9,CYRFA21-1 and VE GF in each group,the cisplatin treatment effect was divided into 15 patients and 37 patients in the treatment-effective group,and 78 patients in the treatment-effective group to clarify the relationship between the treatment effect of patients with non-small-cell lung cancer.Results Serum levels of MMP-9,CYRFA21-1 and VEGF were decreased in NSCLC patients after cisplatin treatment(P＜0.05).Compared with the ineffective group,serum MMP-9,CYRFA21-1 and VEGF levels in the significantly effective group and the effective group were decreased(P＜0.05).Logistic regression analysis showed that MMP-9,CYRFA21-1 and VEGF were also risk factors affecting cisplatin treatment effect in NSCLC patients(P＜0.05).ROC analysis showed that serum MMP-9,CYRFA21-1 and VEGF had high predictive value for cisplatin treatment in NSCLC patients(P＜0.05).Conclusions MMP-9,CYRFA21-1 and VEGF are highly expressed in the serum of patients with severe pneumonia,and the expression levels of MMP-9,CYRFA21-1 and VEGF are decreased after cisplatin treatment in patients with non-small cell lung cancer,which is related to the cisplatin treatment effect in patients with non-small cell lung cancer.Clinical monitoring of serum levels of MMP-9,CYRFA21-1 and VEGF can predict the therapeutic effect early to improve the prognosis of patients.

Chimeric antigen receptor T-cell (CAR-T) immunotherapy has made a breakthrough in the clinical treatment of a variety of hematological tumors.However, the CAR-T cell products listed at China and abroad are all autologous CAR-T.Compared with autologous CAR-T treatment, universal CAR-T exhibits significant advantages, which could fulfill the treatment demand of more patients, but also displays high technical barriers.This paper reviews the universal CAR-T, clearly points out the two major challenges faced by the development of universal CAR-T, and then summarizes and analyzes the feasible solutions according to the mechanism causing the two major problems.This paper also summarizes domestic and foreign companies producing universal CAR-T and the latest clinical progress of their superior products, and then discusses the feasibility of the development strategy from another aspect, in order to provide ideas for developing a new generation of universal CAR-T cell therapy products.

As a novel and promising antitumor target, AXL plays an important role in tumor growth, metastasis, immunosuppression and drug resistance of various malignancies, which has attracted extensive research interest in recent years. In this study, by employing the structure-based drug design and bioisosterism strategies, we designed and synthesized in total 54 novel AXL inhibitors featuring a fused-pyrazolone carboxamide scaffold, of which up to 20 compounds exhibited excellent AXL kinase and BaF3/TEL-AXL cell viability inhibitions. Notably, compound 59 showed a desirable AXL kinase inhibitory activity (IC₅₀: 3.5 nmol/L) as well as good kinase selectivity, and it effectively blocked the cellular AXL signaling. In turn, compound 59 could potently inhibit BaF3/TEL-AXL cell viability (IC₅₀: 1.5 nmol/L) and significantly suppress GAS6/AXL-mediated cancer cell invasion, migration and wound healing at the nanomolar level. More importantly, compound 59 oral administration showed good pharmacokinetic profile and in vivo antitumor efficiency, in which we observed significant AXL phosphorylation suppression, and its antitumor efficacy at 20 mg/kg (qd) was comparable to that of BGB324 at 50 mg/kg (bid), the most advanced AXL inhibitor. Taken together, this work provided a valuable lead compound as a potential AXL inhibitor for the further antitumor drug development.

The article takes the experiment teaching combining Chaoxing Network Teaching Platform with BOPPPS model of obstetrics and gynecology in Chongqing Medical University as an example, and introduces the six teaching modules in detail that are followed in the mixed teaching mode: bridge in, objective, pre-assessment, participatory learning, post-assessment, and summary. Using the three-in-one assessment method of "process evaluation + incentive evaluation + summative evaluation", the learning effect of students was comprehensively evaluated. The practice proved that this mode can improve students' learning autonomy, exercise communication skills, cultivate teamwork spirit, promote the construction of clinical thinking, and improve teaching effect and classroom teaching quality.

Sodium alginate (SA) is a kind of natural polymer material extracted from kelp, which has excellent biocompatibility, non-toxicity, biodegradability and abundant storage capacity. The formation condition of sodium alginate gel is mild, effectively avoiding the inactivation of active substances. After a variety of preparation methods, sodium alginate microspheres are widely used in the fields of biomaterials and tissue engineering. This paper reviewed the common methods of preparing alginate microspheres, including extrusion, emulsification, electrostatic spraying, spray drying and coaxial airflow, and discussed their applications in biomedical fields such as bone repair, hemostasis and drug delivery.
Alginates ; Biocompatible Materials ; Drug Delivery Systems ; Microspheres ; Plastic Surgery Procedures

Objective To explore the polymorphism of microbial community after laparoscopic abdominal exploration by using bacterial 16s ribosomal DNA (16S rDNA) sequencing technology. Methods New Zealand rabbits were divided into model group and control group. The rabbits in the model group were operated by portable laparoscopy, and the rabbits in the control group were not treated. One week later, the peritoneal effusions of the model group and the control group were taken for 16S rDNA sequencing to analyze the microbial community polymorphism. To explore the changes of microbial community in peritoneal effusion in the model group compared with the control group. Results After 16S rDNA sequencing, bioinformatics was used to determine the microbial communities. Inter group difference analysis showed a good similarity of microbial communities between the two groups. OTU taxonomic analysis and species composition analysis (Rank-Abundance curve and Venn diagram) found that the microbial community level of the model group was significantly higher than that of the control group. Alpha diversity analysis (Sobs, Ace, Shannon, Simpson) showed that the richness and diversity of microbial community in the model group were higher than those in the control group. Microbial composition analysis showed that the number of miscellaneous bacteria in the model group increased by about 30% compared with the control group. The species differences between the two groups were tested for significance. It was found that Pasteurellales, Neisseria and Tsukamurella increased significantly. Conclusion The diversity of microbial communities in peritoneal effusion increases after laparoscopic abdominal exploration in New Zealand rabbits, and the most significant increases are Pasteurella, Neisseria and Tsukamura.

Objective To investigate the relationship between histological chorioamnionitis(HCA) and the outcome of preterm labor. Methods A total of 218 cases of premature delivery in the Department of Obstetrics and Gynecology of our hospital from January, 2015 to December, 2017 were divided into two groups according to the result of placenta pathological diagnosis. The observation group was diagnosed with histological chorioamnionitis by placenta pathology examination. The control group was not diagnosed with histological chorioamnionitis. The delivery gestational week, the rate of premature rupture of membranes, the rate of puerperal infection, the rate of postpartum hemorrhage, the rate of wound healing failure, the average body mass of newborn infants, the rate of early-onset sepsis, asphyxia, respiratory distress syndrome rate, pathological jaundice rate, neonatal mortality between the two groups were compared. And the impact of histological chorioamnionitis on the outcome of preterm labor was explored. Results The delivery gestational week of the observation group was (32. 0±1. 0) weeks, and the rate of postpartum hemorrhage, the rate of premature rupture of membranes, the rate of puerperal infection and the rate of wound healing were 21. 24％, 80. 50％, 16. 81％, 11. 50％, respectively. The delivery gestational week of the control group was(34. 0±1. 0) weeks, and the rate of postpartum hemorrhage, the rate of premature rupture of membranes, the rate of puerperal infection and the rate of wound healing were 10. 48％, 65. 70％, 7. 62％, 3. 81％, respectively. The delivery gestational week of the observation group was small. And the rate of premature rupture of membranes, wound healing failure rate, puerperal infection rate, postpartum hemorrhage rate in the observation group was significantly higher than that of the control group, and the difference was statistically significant(P＜0. 05). The mean neonatal weight in the observation group was(1710±355)g,and the asphyxia rate, early-onset sepsis rate, respiratory distress syndrome rate, pathological jaundice rate and neonatal mortality were 21. 24％, 33. 63％, 38. 05％, 19. 47％ and 9. 73％. The mean neonatal weight in the control group was(2270±450)g,and the asphyxia rate, early-onset sepsis rate, respiratory distress syndrome rate, pathological jaundice rate, neonatal mortality were 9. 52％, 18. 10％, 12. 38％, 8. 57％, 2. 86％. The average body weight of infants in the observation group was low. And the rates of early onset sepsis, asphyxia, respiratory distress syndrome, pathological jaundice and neonatal mortality were significantly increased. The difference was significant (P＜0. 05). Conclusion The placental pathology examination in all preterm patients should be done to avoid missed diagnosis of HCA, and to help early diagnosis and treatment of pregnant women with intrauterine infection and high-risk newborns and improve prognosis.

Objective To explore the effects of follow-up management based on structural family therapy on self efficacy and quality of life in discharged puerpera. Methods A total of 124 hospitalized puerpera with childbirth of the Affiliated Hospital of Medical School of Ningbo University were selected from October 2015 to October 2016. All of them were divided into observation group and control group, 62 cases in each group. Puerpera of control group were treated with conventional nursing. On the basis of the conventional nursing, puerpera of observation group adopted follow-up management based on structural family therapy. We compared the scores of breast feeding knowledge questionnaire, the rate of exclusive breastfeeding and the quality of life with the Exercise of Self-Care Agency Scale (ESCA) and the quality of life index scale of puerpera in two groups. Results The mean score of breast feeding knowledge questionnaire of puerpera in the observation group was higher than that in the control group with a significant difference (P<0.05). The rate of exclusive breastfeeding of puerpera in the observation group was 93.54% higher than that in the control group (74.19%) with a significant difference (P<0.05). There was a statistically significant difference in the total score of self efficacy of puerpera between the observation group and the control group [(143.4±14.0) vs. (109.6±11.6)] (P< 0.05). The total score of quality of life of puerpera in the observation group was significantly higher than that in the control group [(8.8±1.3) vs. (5.6±1.1)](P< 0.05). Conclusions The follow-up management based on structural family therapy is favourable to improve the self efficacy on nursing and quality of life of puerpera so as to increase the rate of breast feeding.

Objective To discuss the effect of 5-Aza-CdR on pancreatic carcinoma Capan-2 cell proliferation and PCDH8 gene expression.Methods Pancreatic carcinoma Capan-2 cells were treated with different doses of 5-Aza-CdR with or without gemcitabine,negative control group without drug,0.08μmol/L group with 0.08μmol/L 5-Aza-CdR,0.40 μmol/L group with 0.40 μmol/L 5-Aza-CdR,2.00 μmol/L group with 2.00 μmol/L 5-Aza-CdR,10 μmol/L group with 10 μmol/L 5-Aza-CdR,50 μmol/L group with 50 μmol/L 5-Aza-CdR. The inhibition ratio of Capan-2 cell proliferation were observed by MTT assay and PCDH8 gene and protein expression were detected by RT-PCR and Western blot. Results The inhibition ratio was increased with 5-Aza-CdR dose increasing(P<0.01),but decreased apparently with times extending(P<0.01). Inhibition ratio in 5-Aza-CdR and gemcitabine group was higher than those with only 5-Aza-CdR or gemcitabine groups(P<0.05 or P<0.01).The levels of PCDH8 gene and protein expression were increased significantly in 5-Aza-CdR treatment groups,with dose dependent (P <0.01 ).Conclusion 5-Aza-CdR can inhibit the proliferation of pancreatic carcinoma Capan-2 cell proliferation,and increase PCDH8 gene expression. The inhibition effect is strong when combined with gemcitabine.