[Objective] To analyze the mediating pathways of autonomy perception on the intrinsic motivation for blood donation and the moderating mechanism of satisfaction, and to explore the factors that enhance the intrinsic motivation of blood donation in order to promote sustained donor engagement and effectiveness. [Methods] This study, grounded in Self-Determination Theory (SDT), recruited 1 293 employees from private enterprises in Zhejiang Province with prior blood donation experience as the research subjects. Data on demographic characteristics, intrinsic motivation for blood donation, and influencing factors were collected through a voluntary blood donation questionnaire. Spearman correlation analysis and the PROCESS macro were employed to examine the relationships among variables and their underlying mechanisms. [Results] In the multiple linear regression analysis with autonomy perception, life balance perception, and satisfaction as independent variables, 60% of the variance in intrinsic motivation for blood donation was explained by these predictors (adjusted R²=0.60). In the moderated mediation model test, life balance perception mediated the effect of autonomy perception on intrinsic motivation for blood donation. Furthermore, blood donation satisfaction positively moderated the direct path (β=0.06, P<0.01) while negatively moderating the second half of the mediation path (β=-0.04, P<0.01), revealing a dual moderating effect of satisfaction. [Conclusion] Autonomy perception and blood donation satisfaction jointly drive intrinsic motivation for blood donation. Strategies for optimizing blood donation satisfaction, which take into account both the direct path effect and the indirect mediation effect, can offer valuable insights for addressing the discrepancy between high first-time donation rates and low donor retention rates.

Nutritional support therapy is one of the extremely important treatment methods for patients in the intensive care unit. Timely and effective nutritional support regimens can improve patients' immune function, reduce complications, and optimize clinical outcomes. Energy expenditure is influenced by multiple factors, including patients' baseline characteristics (such as physical condition, gender, age) and dynamic changes in indicators (such as body temperature, nutritional support regimens, and therapeutic interventions). The currently recognized "gold standard" for accurately assessing energy metabolism in clinical practice is the indirect calorimetry system, also known as the metabolic cart. This device monitors carbon dioxide production and oxygen consumption in real time and uses specific algorithms to estimate the metabolic proportions of the three major nutrients (carbohydrates, fats, and proteins) in energy expenditure. An appropriate nutrient ratio helps maintain the balance between supply and demand in the body's nutritional metabolism. In the management of critically ill patients, the application of the metabolic cart enables personalized nutritional therapy, avoiding over- or under-supply of energy and optimizing the use of medical resources. Furthermore, with real-time, quantitative data support from the energy metabolism monitoring system, clinicians can develop more precise nutritional intervention strategies, thereby improving patient prognosis. This article provides a systematic review of the technical features of the metabolic cart and its application value in various critical care scenarios, aiming to offer a reference for indirect calorimetry in clinical practice.
Humans ; Critical Illness/therapy* ; Nutritional Support ; Energy Metabolism ; Calorimetry, Indirect

Objective To investigate the role and mechanism of bergapten in treating osteoarthritis(OA)based on network pharmacology,molecular docking and in vitro experiments.Methods The functional targets of bergapten and the related targets of OA disease were obtained by searching Super-Pred,Swiss Target Prediction,Drug Bank.The obtained targets were intersected with Venny2.1.0,protein-protein interaction(PPI)analysis was performed using the STRING database,and molecular docking was conducted by AutoDock Vina.Subsequently,human chondrocyte C28/I2 cells were subjected in the following validation experiments.After the cells were treated with 0～50 μmol/L bergapten for 24,48 or 72 h,CCK-8 assay,RT-qPCR and immunofluorescence assay were used to detect the proliferation and apoptosis,and the expression of the molecules related to proliferation,inflammation and senescence(senescence associated secretory phenotype,SASP).Senescence β-galactosidase(SA-β-Gal)staining kit was employed to detect the change of SA-β-Gal level in the cells.Results There obtained 145 potential targets of bergapten for OA.PPI analysis revealed 4 potential core targets,HSP90AA1,EGFR,HIF1A,and PIK3CA,and they could form relatively stable complex with bergapten.CCK-8 assay showed that 10 μmol/L bergapten treatment for 48 h resulted in the highest proliferative activity of C28/12 cells(P＜0.05).Immunofluorescence assay,RT-qPCR and Western blotting indicated that bergapten treatment induced significant increases in the expression of cell proliferation markers such as PCNA,CCND1 and CCNE1(P＜0.05),while decreases in the expression of apoptosis markers Caspase3 and BAX(P＜0.05).Meanwhile,the expression levels of IL-1β-induced inflammatory factors IL-6 and TNF-α were reduced,while those of anti-inflammatory factors IL-4 and IL-10 were elevated after bergapten treatment(P＜0.05).The levels of SASP factors,such as P16,P21,MMP9 and MMP13 were also significantly declined(P＜0.05).SA-β-Gal staining displayed that the level of SA-β-Gal in C28/I2 cells was significantly enhanced after IL-1β induction(P＜0.05),while bergapten treatment could decrease the positive cell rate of the staining(P＜0.05).Conclusion Bergapten can reduce the expression of SASP factors such as IL-6,TNF-α,P16,P21,MMP9 and MMP13 in IL-1 β-stimulated chondrocytes,and thus exerts its anti-inflammatory and anti-aging effects in delaying the progression of OA.

Objective To develop and validate a prediction model for risk of amputation in patients with diabetic foot ulcers(DFU)based on systematic review and meta-analysis.Methods The studies on the risk factors of amputation in DFU patients was retrieved by using subject words+free words.After screening,37 cohort studies were finally included,and the Newcastle-Ottawa scale(NOS)was used for quality evaluation.Meta-analysis was performed on the risk factors of amputation in DFU.Then a prediction model for DFU amputation risk were constructed based on the statistically significant risk factors in the meta-analysis.The corresponding β value was calculated based on the combined odds ratio(OR)value of each risk factor,and each risk factor was scored to establish a scoring system model.The clinical data of 453 DFU patients hospitalized in our department from 2021 to 2023 were collected as a validation cohort.Receiver operating characteristic(ROC)curve analysis was used to evaluate the model performance.The area under the curve(AUC)was calculated,and the optimal cutoff score was determined by calculation of the maximum Youden index through sensitivity and specificity.Results Our meta-analysis showed a cumulative amputation rate of approximately 34.65%in 11 779 DFU patients.The final risk prediction models include gangrene[OR=11.92(5.86～24.24)],ulcer depth[OR=4.93(2.52～9.64)],osteomyelitis[OR=3.19(2.36～4.29)],previous amputation history[OR=3.19(2.00～5.09)]and lower extremity arterial disease[OR=3.10(2.31～4.17)].According to the weights of each risk factor,the total score of the model is 76,and the optimal cut-off score is 36.5.The prediction model performed well,with an AUC value of 0.864(0.824,0.903),a sensitivity of 0.743,a specificity of 0.859,and an accuracy rate of 83.00%.Conclusion A prediction model for DFU amputation risk is developed based on risk factor scoring,and has good discrimination and calibration,providing effective scientific basis for clinical research and clinical decision-making related to DFU amputation.

Currently, transcatheter intervention is the preferred treatment for patients with anatomically suitable atrial septal defects. However, the use of nickel-titanium alloy occluders in interventional procedures results in lifelong presence of the implant in the body, leading to complications such as metal allergies and arrhythmias in some patients. To overcome the short-term and long-term complications associated with the presence of metal, and to avoid radiation exposure and metal toxicity, this paper reports a case of successful transcatheter closure of atrial septal defect in a pediatric patient with metal allergies using fully biodegradable occluder under ultrasound guidance, achieving excellent results by interventional therapy.

This article summarizes and organizes relevant publications in journals, along with a review of medical history, systematically summarizing the development process of dental alveolar surgery in China. The initial establishment phase (1935—1952) marked the starting point of Chinese Alveolar Surgery. Despite the impact of wars, it laid the foundation for subsequent research and practice. During the early development phase (1953—1966), the "Chinese Journal of Stomatology" was founded, which promoted the development of Alveolar Surgery. Research focused on tooth extraction methods and complications. Tooth Transplantation and Preprosthetic Surgery gradually began to take off. The stagnant phase (1967—1977) occurred due to the interruption of international exchanges, leading to an almost complete halt in the development of Alveolar Surgery. Entering the rapid catch-up phase (1978—1985), Alveolar Surgery scholars in China began striving to overcome the stagnation of the previous decade. While some progress was made, no significant innovative achievements emerged. In the scientific development phase (1986—2010), clinical research, basic experiments, and paper writing in modern Chinese Alveolar Surgery began to adhere to scientific standards with the rise of experimental medicine. The exploration and innovation stage (2011—2023) is the current development phase, during which Chinese Aveolar Surgery has reached its peak, making substantial progress in technology, clinical practices, and basic research, gradually reaching or even surpassing international advanced levels. Looking back at the development history in China, we can find the wisdom and hard work of the older generation of Alveolar Surgery scholars. However, contemporary challenges and issues, such as standardizing technology, promoting clinical practices, and talent cultivation, need to be addressed by present-day Alveolar Surgery professionals as they forge ahead.

Objective Recurrent pregnancy loss(RPL)presents a formidable challenge for individuals undergoing in vitro fertilization-embryo transfer(IVF-ET),forming both a clinical dilemma and a focal point for scientific inquiry.This study endeavors to investigate the intricate interplay between clinical features,such as age,body mass index(BMI),and waist-to-hip ratio(WHR),and routine laboratory parameters,including sex hormones,blood composition,liver and thyroid functions,thyroid antibodies,and coagulation indicators,in RPL patients undergoing IVF-ET.By meticulously analyzing these variables,we aim to uncover the latent risk factors predisposing individuals to RPL.Identifying potential factors such as advanced maternal age,obesity,and insulin resistance will provide clinicians with vital insights and empirical evidence to strengthen preventive strategies aimed at reducing miscarriage recurrence.Methods This retrospective case-controlled study included RPL patients who underwent IVF-ET treatment at Sun Yat-sen Memorial Hospital,Sun Yat-sen University,between January 2012 and March 2021 as the case cohort,compared with women receiving assisted reproductive treatment due to male infertility as the control cohort.The fasting peripheral blood was collected 5 days before the first menstrual cycle at least 12 weeks after the last abortion.The clinical characteristics and relevant laboratory indexes of the two groups were compared.Employing both univariate and multivariate logistic regression analyses,we sought to unearth potential high-risk factors underlying RPL.Additionally,a linear trend analysis was conducted to assess the linear relationship between total testosterone(TT)levels and the number of miscarriages.Results In contrast to the control cohort,the RPL cohort exhibited significant increases in age,BMI,and WHR(P<0.05).Notably,TT levels were markedly lower in the RPL cohort(P=0.022),while no significant differences were observed between the two groups concerning basal follicle-stimulating hormone,luteinizing hormone,estradiol,progesterone,prolactin levels,and anti-Müllerian hormone levels(P>0.05).Moreover,fasting insulin(FINS)levels and HOMA-IR index were notably elevated in the RPL cohort relative to the control cohort(P<0.001),although no significant differences were observed in fasting blood glucose levels(P>0.05).Furthermore,the neutrophil(NEU)count and NEU-to-lymphocyte ratio were notably higher in the RPL cohort(P<0.01).Univariate logistic regression analysis identified several factors,including age≥35 years old,BMI≥25 kg/m2,WHR>0.8,FINS>10 mU/L,HOMA-IR>2.14,NEU count>6.3×109 L-1,and an elevated NEU/lymphocyte ratio(NLR),as significantly increasing the risk of RPL(P<0.05).Although TT levels were within the normal range for both cohorts,higher TT levels were associated with a diminished RPL risk(odds ratio[OR]=0.67,95% confidence interval[CI]:0.510-0.890,P=0.005).After adjustments for confounding factors,age≥35 years old(OR=1.91,95% CI:1.06-3.43),WHR>0.8(OR=2.30,95% CI:1.26-4.19),and FINS>10 mU/L(OR=4.50,95% CI:1.30-15.56)emerged as potent risk factors for RPL(P<0.05).Conversely,higher TT levels were associated with a reduced RPL risk(OR=0.59,95% CI:0.38-0.93,P=0.023).Furthermore,the linear trend analysis unveiled a discernible linear association between TT levels and the number of miscarriages(Ptrend=0.003),indicating a declining trend in TT levels with escalating miscarriage occurrences.Conclusion In patients undergoing IVF-ET,advanced maternal age,lower TT levels,increased WHR,and elevated FINS levels emerged as potent risk factors for RPL.These findings provide clinicians with valuable insights and facilitate the identification of patients who are at high risks and the formulation of preventive strategies to reduce the recurrence of miscarriages.

Purpose: In non-metastatic prostate cancer (nmPCa) setting, it is important to early identify the patients at risk of biochemical recurrence (BCR) for immediate postoperative intervention. Our study aimed to evaluate the potential clinical utility of circulating tumor DNA (ctDNA) for predicting disease recurrence. Materials and Methods: This real-world observational study evaluated 161 cases of nmPCa undergoing next-generation sequencing at our institution. A total of 139 ctDNA samples and 31 biopsied tumor tissue underwent genomic profiling. The study endpoint was BCR after radical prostatectomy. Relationships between the ctDNA status and the biochemical progression-free survival (bPFS) were analyzed by log-rank test and multivariate Cox regression. Results: Of 161 enrolled patients, 19 (11.8%) harbored deleterious alterations in NCOR2, followed by BRCA2 (3.7%), ATR (2.5%), and CDK12 (2.5%). Of available pre-operative blood samples (n=139), ctDNA was detectable in 91 (65.5%). Until last follow-up, 56 of 68 patients (85.3%) with detectable ctDNA had achieved BCR, whereas only eight of 39 patients (20.5%) with undetectable ctDNA had achieved BCR. Patients who had undetectable ctDNA experienced significantly longer bPFS compared with those who had detectable ctDNA (not available vs. 8.2 months; hazard ratio, 0.14; p < 0.01). Pre-operative ctDNA status was a significant prognostic factor of disease recurrence. Conclusion Pre-operative ctDNA detection could identify patients at high risk of recurrence and has the potential to inform immediate postoperative interventions, but these approaches remain to be validated in prospective studies. ctDNA studies can provide insights into accurate monitoring and precise treatment rather than simply following routine clinical care.

【Objective】 To explore the recruitment strategy of blood donors under the situation of blood supply shortage during the COVID-19 epidemic. 【Methods】 The theory of planned behavior and the stage change model of behavior were used to analyze the recruitment strategy of blood donors during the COVID-19 epidemic. The recruitment practice strategies of some countries or regions, i. e. the United States, Japan, South Korea, and the European Union, during this period were investigated. Based on the theoretical analysis results and practical status, relevant reference strategies were proposed. 【Results】 The theoretical analysis showed that attitude, subjective norms and ethical norms played an important role in the recruitment of blood donors during the COVID-19 epidemic, but blood donation self-efficacy and promoting blood donation behavior were the two key factors. According to the survey, in terms of enhancing and maintaining attitudes, subjective norms, ethical norms and other elements that were jointly affected by the same strategy, most countries continued previous policies while some updated recruitment ideas. In terms of enhancing and maintaining the key elements of blood donation self-efficacy, more human and material support had been made. 【Conclusion】 During the COVID-19 pandemic, enhancing and maintaining blood donation self-efficacy and promoting blood donation behavior are the key elements to effectively improve the blood supply shortage. The blood donor recruitment strategy constructed accordingly has reference value for China.

OBJECTIVE: To investigate the regulatory role of the long non-coding RNA LINC00926 in pyroptosis of hypoxia-induced human umbilical vein vascular endothelial cells (HUVECs) and explore the molecular mechanism. METHODS: HUVECs were transfected with a LINC00926-overexpressing plasmid (OE-LINC00926), a siRNA targeting ELAVL1, or both, followed by exposure to hypoxia (5% O2) or normoxia. The expression of LINC00926 and ELAVL1 in hypoxia-treated HUVECs was detected using real-time quantitative PCR (RT-qPCR) and Western blotting. Cell proliferation was detected using Cell Counting Kit-8 (CCK-8), and the levels of IL-1β in the cell cultures was determined with ELISA. The protein expression levels of pyroptosis-related proteins (caspase-1, cleaved caspase-1 and NLRP3) in the treated cells were analyzed using Western blotting, and the binding between LINC00926 and ELAVL1 was verified with RNA immunoprecipitation (RIP) assay. RESULTS: Exposure to hypoxia obviously up-regulated the mRNA expression of LINC00926 and the protein expression of ELAVL1 in HUVECs, but did not affect the mRNA expression of ELAVL1. LINC00926 overexpression in the cells significantly inhibited cell proliferation, increased IL-1β level and enhanced the expressions of pyroptosis-related proteins (all P < 0.05). LINC00926 overexpression further up-regulated the protein expression of ELAVL1 in hypoxia-exposed HUVECs. The results of RIP assay confirmed the binding between LINC00926 and ELAVL1. ELAVL1 knockdown significantly decreased IL-1β level and the expressions of pyroptosis-related proteins in hypoxia-exposed HUVECs (P < 0.05), while LINC00926 overexpression partially reversed the effects of ELAVL1 knockdown. CONCLUSION LINC00926 promotes pyroptosis of hypoxia-induced HUVECs by recruiting ELAVL1.
Humans ; Caspase 1 ; ELAV-Like Protein 1 ; Human Umbilical Vein Endothelial Cells ; Pyroptosis ; RNA, Messenger ; RNA, Long Noncoding/genetics* ; Cell Hypoxia