Objective To explore the effect of long non-coding RNA(long non-coding RNA,lncRNA)small RNA host gene 15(small nucleolar RNA host gene 15,SNHG 15)regulating microRNA 95-3p(miR-95-3p)on breast cancer cell proliferation and apoptosis.Methods Breast cancer cells were divided into control group,knockdown group,interference group and co-transfection group.Human breast cancer cell line MDA-MB-231 was cultured in vitro.Measure the relative expression levels of SNHG 15 mRNA and miR-95-3p mRNA;Methylthiazolyldiphenyl-tetrazolium bromide(MTT)was used to detect the proliferation ability of breast cancer cells;The apoptosis of breast cancer cells was detected by flow cytometry;The migration ability of breast cancer cells was detected by scratch test;Transwell invasion test was used to measure the number of breast cancer cells invaded;Western blot was used to determine the relative expression levels of caspase-3,B-cell lymphoma-2(Bcl-2),and Bcl-2 associated X protein(Bax).Results Compared with control group,the relative expression level of SNHG 15 mRNA and miR-95-3p mRNA,proliferation ability,proliferation rate,migration ability,number of invasion,Bcl-2 protein expression were decreased,but the apoptosis status,apoptosis rate,caspase-3 and Bax expression were increased in knockdown group(P＜0.05).Compared with knockdown group and interference group,the relative expression level of SNHG 15 mRNA and miR-95-3p mRNA,proliferation capacity,proliferation rate,migration capacity,number of invasion,Bcl-2 protein expression were increased,but apoptosis status,apoptosis rate,caspase-3 and Bax expression were decreased in co-transfection group(P＜0.05).Compared with interference group,the relative expression level of SNHG 15 mRNA and miR-95-3p mRNA,proliferation ability,proliferation rate,migration ability,number of invasion,Bcl-2 protein expression were increased,but apoptosis status,apoptosis rate,caspase-3 and Bax expression were decreased in co-transfection group(P＜0.05).Conclusion lncRNA SNHG 15 can affect the proliferation and apoptosis of breast cancer cells by regulating miR-95-3p expression,and is positively associated with the occurrence and development of breast cancer cells.

Objective: In order to understand temporal and spatial distributions of alternating electromagnetic field (AEMF) of a coil thoroughly, the 3-D finite-element models of this kind of Biomagnetic Fields are established and analyzed for the research on bioeffect of magnetic field on microcirculation. Methods: Designing CAD models, setting boundary conditions, meshing models and obtaining numerical solutions were completed all within Comsol Multiphysics in terms of the theory of electromagnetic field. Results: Axial and radial plot of spatial attenuation rate of the AEMF, which were accordance with measured data, were simulated while the temporal and spatial distributions of AEMF were acquired. Conclusion: Models of 3-D finite-element of this kind of biomagnetic fields which appeared to exactly simulate the real magnetic field were first established systematically, and solved the placing problem of magnetic fields for the research on bioeffect of magnetic field on microcirculation and provided platforms for the simulation of hemodynamic of blood flow in the magnetic field.

Most of the CHD patients reveal string pulse, mainly due to damage of heart function, lowering of arterial compliance and increase of total peripheral resistance. The common pulse in patients of blood diseases reveal frequent, tiny, string and slippery characteristic, mostly due to the increase of compensatory pumping action of the heart, shortening of ejection time of the left heart, with better vessel compliance and hemorheology, low total peripheral resistance.