Objective:To investigate the specific role of circTRRAP in acute myocardial infarction(AMI).Methods:The method of hy-poxia for 24 hours was used to induce the model of myocardial infarction,and the dual-luciferase reporter assay was used to investigate the interaction between circTRRAP,SMAD2,and miR-323-3p.After knockdown and overexpression of miR-323-3p and overexpres-sion of SMAD2 through transfection with si-circTRRAP,the expression levels of proinflammatory cytokines[interkeukin-6(IL-6)and tumor necrosis factor-α(TNF-α)],oxidative stress markers[malondialdehyde(MDA)and superoxide dismutase(SOD)],and apop-totic factors[Bcl-2-associated X protein(Bax),B-cell lymphoma-2(Bcl-2),and cleaved caspase-3]were measured to investigate the role of circTRRAP,SMAD2,and miR-323-3p in myocardial infarction.Results:In the model of myocardial infarction injury,the lev-els of circTRRAP and SMAD2 were significantly increased by more than 50%,whereas there was a significant reduction in the expres-sion of miR-323-3p.The downregulation of circTRRAP led to a reduction in SMAD2 expression by promoting miR-323-3p expres-sion.SMAD2 was negatively correlated with miR-323-3p,but it was positively correlated with the expression of circTRRAP.The down-regulation of circTRRAP or SMAD2 or the upregulation of miR-323-3p could increase cell viability and reduce the apoptosis rate of cardiomyocytes.Conclusion:Downregulation of circTRRAP can inhibit inflammation and alleviate AMI via the miR-323-3p/SMAD2 axis.

BACKGROUND:Studies have shown that both Panax notoginseng saponins and concentrated growth factor can promote fracture healing,but there are few studies addressing their combined effects on fracture healing.Panax notoginseng saponins may accelerate fracture healing by promoting the release of concentrated growth factor-related factors over a certain period of time. OBJECTIVE:To study the effect of Panax notoginseng saponins on concentrated growth factor release and fracture healing in rats. METHODS:Eighteen 8-week-old Sprague-Dawley rats were numbered and randomly divided into three groups:Panax notoginseng saponins group,model control group and blank group.Panax notoginseng saponins group was fed with Panax notoginseng saponins for 2 weeks.Model control group was given 2 mL of normal saline for 2 weeks and blank group was fed normally.Concentrated growth factor was obtained by the centrifugation method both from the Panax notoginseng saponins group and model control group.After 1 week of normal feeding,all animals underwent modeling for femoral fracture.The Panax notoginseng saponins group and the model control group were implanted with autologous concentrated growth factor,and then the release concentration of growth factors at different time points(1 hour,1,3,5,7,9 and 11 days)were measured by ELISA.Fracture healing was assessed based on postoperative X-ray and hematoxylin-eosin staining of bone tissues. RESULTS AND CONCLUSION:Compared with the model control group,the Panax notoginseng saponins group had higher release concentrations of vascular endothelial growth factor A and transforming growth factor β at 7,9,and 11 days,Platelet-derived growth factor BB at 5,9,and 11 days,and basic fibroblast growth factor at 1-11 days(P<0.01).X-ray examinations indicated that fracture healing in the Panax notoginseng saponins group was better than that in the model control group,and fracture healing in these two groups was better than that in the blank group at 2 months after surgery.Hematoxylin-eosin staining results found that the constituent osteocyte density in the Panax notoginseng saponins group was greater than that in the model control group,and the constituent osteocyte density in these two groups was better than that in the blank group.These findings indicate that Panax notoginseng saponins can increase the concentration of concentrated growth factor-related factors.After intervention with Panax notoginseng saponins,concentrated growth factors are more advantageous in promoting fracture healing in rats.

Objective To analyze the differences in computed tomography(CT)myocardial perfusion parameters between type 2 diabetes mellitus(T2DM)patients and nondiabetic patients diagnosed with non-obstructive coronary artery disease(CAD),using a one-stop cardiac CT scanning protocol that combines coronary CT angiography(CCTA)with dynamic CT myocardial perfusion imaging(CT-MPI).In addition,we investigated the effect of T2DM on coronary microcirculatory ischemia.Methods After balancing the baseline levels with propensity score matching,92 T2DM patients(the T2DM group)and 92 nondiabetic patients(the nondiabetic group)with non-obstructive CAD were enrolled eventually.The clinical characteristics and the CCTA and CT-MPI results of the two groups were compared.A directed acyclic graph was used to analyze the causal relationships between the variables and to identify key confounding factors.A multivariable regression model was established to evaluate the independent effect of T2DM on the occurrence of coronary microcirculatory ischemia after adjusting for confounding factors.Results There were no statistically significant differences between the T2DM group and the nondiabetic group in terms of age,sex,hypertension,hyperlipidemia,smoking history,body mass index,chest symptoms,calcium score,CAD-reporting and data system(CAD-RADS)score,and radiation dose.In the T2DM group,the mean values of myocardial blood flow(MBF)were significantly reduced both globally and in all myocardial segments(basal,mid,and apical segments)compared to those of the nondiabetic group(P＜0.05).Furthermore,the incidence of coronary microcirculatory ischemia in the T2DM group was significantly higher than that in the nondiabetic group(21.7％[20/92]vs.5.4％[5/92],P=0.01).Multivariable logistic regression analysis showed that T2DM was an important independent risk factor for coronary microcirculatory ischemia(odds ratio=5.095,95％confidence interval:1.753-14.805).Conclusion According to our assessment with a one-stop cardiac CT scanning protocol combining CCTA and dynamic CT-MPI,patients with non-obstructive CAD and T2DM have reduced global MBF,which makes them more prone to coronary microcirculatory ischemia.Furthermore,T2DM is independently associated with coronary microcirculatory ischemia.

In order to improve the efficiency of protein spots detection, a fast detection method based on CUDA was proposed. Firstly, the parallel algorithms of the three most time-consuming parts in the protein spots detection algorithm: image preprocessing, coarse protein point detection and overlapping point segmentation were studied. Then, according to single instruction multiple threads executive model of CUDA to adopted data space strategy of separating two-dimensional (2D) images into blocks, various optimizing measures such as shared memory and 2D texture memory are adopted in this study. The results show that the operative efficiency of this method is obviously improved compared to CPU calculation. As the image size increased, this method makes more improvement in efficiency, such as for the image with the size of 2,048 x 2,048, the method of CPU needs 52,641 ms, but the GPU needs only 4,384 ms.
Algorithms ; Image Processing, Computer-Assisted ; Proteomics ; methods ; Software

There are no intuitive and unified standards for selecting features in existing protein gel image point matching method, for which an analysis method based on similarity map is proposed. Firstly, the definition and generation methods of similarity map were presented. Secondly, trait and merits of features such as coordinate similarity, shape-context similarity and morphology similarity were analyzed using similarity map method. Finally, comprehensive utilization of multi-features named product-method which has a better effect than mean-method was proposed based on the results. Many experiments using different 2-DE gel images were carried out to prove the validity of similarity map and product-method. The results showed that similarity map could be used for intuitional, effective analysis of matching performance and to guide the selection and comprehensive utilization of multi-features.