Background Frailty serves as a significant precursor to falls, disability, and mortality. Epidemiological evidence examining the impact of working hours on frailty remains scarce. Objective To explore the prevalence of frailty and the relationship between frailty and working hours among full-time middle-aged and older workers in China. Methods Data were derived from the 2020 China Health and Retirement Longitudinal Study (CHARLS). The study included full-time workers aged 45 years and above with a weekly working duration exceeding 35 h. Frailty was assessed using the Frailty Index (FI). First, the dose-response relationship between working hours and FI was explored using a generalized additive model (GAM). Second, univariate analyses were performed using t-tests, χ² tests, and the Jonckheere–Terpstra trend test. Restricted cubic splines (RCS) were introduced for modeling. Based on ordinal logistic regression models, covariates were adjusted sequentially to examine whether differences in frailty prevalence existed across different weekly working hour categories. Finally, subgroup analyses were performed. Results Among the 5504 included participants, the mean age was (56.41±7.30) years, with 2304 being female (41.86%). A total of 807 participants (14.66%) were identified as frail, and 2 686 participants (48.80%) were pre-frail. After standardization for gender and age according to the seventh national census data, the standardized prevalence of frailty and prefrailty were 18.89% and 47.25%, respectively. Significant higher proportions of frail individuals were found among those in non-managerial (73.73%), agricultural (64.06%), and non-employed (65.68%) occupations. Long working hours were prevalent: 2596 (47.17%) workers worked 41–60 h per week, 1649 (29.96%) worked >60 h per week, and only 1259 (22.87%) worked 35–40 h per week. GAM analysis showed a non-linear relationship between weekly working hours and FI. Restricted cubic spline analysis showed a statistically significant association between weekly working hours and frailty status (P=0.022). Logistic regression analysis revealed that individuals working more than 60 h per week had a 23% higher odds of frailty compared to those working 41–60 h (OR=1.23, 95%CI: 1.09, 1.38, P<0.01), while those working 35–40 h per week showed a 15% higher odds of frailty (OR=1.15, 95%CI: 1.01, 1.31, P=0.034). The subgroup analysis revealed that the positive association between working more than 60 h per week and frailty remained generally consistent across sex, age, urban/rural residence, occupational attributes, industry, or employment type, although the association did not reach statistical significance among non-rural residents and individuals aged 60 years and older. Conclusion The prevalence of frailty among full-time middle-aged and older labor force in China is relatively high, and the odds of frailty increases significantly among those who work more than 60 h per week. It is recommended to implement frailty prevention and management strategies targeting long-working-hour workers and to prioritize the impact of working duration on frailty.

Rectal cancer is a prevalent malignant tumor both in China and worldwide. Despite the availability of numerous sphincter-preserving procedures for low rectal cancer, each with specific indications, advantages, and limitations, no single procedure achieves optimal outcomes in all key domains: anal function preservation, surgical complication reduction, local recurrence rate minimization, and patient quality of life improvement. This article reviewed the evolutionary trajectory of laparoscopic sphincter-preserving surgery for low rectal cancer, while addressing current surgical challenges including prevention and management of anastomotic leakage, strategies for ensuring adequate distal resection margins, and functional outcomes prognosis. Corresponding countermeasures for these critical issues were systematically discussed.

Astrocytes and microglia engage in extensive and complex communication and mutual effect, which referrs to microglia-astrocyte crosstalk. Recent studies have highlighted that this crosstalk plays a pivotal role in neurodegenerative diseases, exerting either protective or detrimental effects. This review briefly introduces the molecular mechanism of microglia-astrocyte crosstalk and its research progress in Alzheimer's disease, multiple sclerosis, amyotrophic lateral sclerosis, and Parkinson's disease, aiming to provide new research directions and therapeutic targets for clinical improvement of neurodegenerative diseases from perspective of microglia-astrocyte crosstalk.

Objective:To investigate the effect and safety of the double-cross dressing technique for composite rhytidectomy.Methods:A retrospective analysis was performed on a total of 121 patients with face and neck ageing, who were admitted to the Facial and Neck Plastic Surgery Center, the Plastic Surgery Hospital of the Chinese Academy of Medical Sciences from June 2020 to May 2023, all female with the age ranged 34-72 years old. All the patients underwent a full facial and neck composite rhytidectomy. Immediately after surgery, a double-cross bandaging was applied. The cotton pad was nested on the outer auricle, so that the front of the ear and the back of the ear were the first cross. This cross aimed to accurately pressurized the temporal, buccal region and postauricle flap. The second cross occurred at the radix. It secured composite tissue flap at the zygomatic arch and the buccal fat pad zone. Patient′s external auricle was not compressed after bandaging. The healing and the occurrence of complications were observed.Results:All the patients were satisfied with the comfort of the bandaging, the satisfied rate was 100% (121/121). All the patients had different degrees of periocular and perioral swelling 72 hours after surgery. Conjunctival edema and xanthochromia occurred in 30 patients, and oral mucosal congestion occurred in 25 patients, all of which were spontaneously resolved 2-4 weeks after surgery. Postoperative nursing was easily managed. There were no events such as dressing loosening, blocked draining tubes, and difficulty in replacing tubes. All the patients were followed up for 3-12 months after surgery, the flap in the postauricular area of all patients healed well, and there were no adverse complications such as wound infection, necrosis of the flap in the operative area, and delayed healing of the incision.Conclusion:The double-cross bandaging technique achieves the comfort bandaging and good protection of the face, which is safe and effective.

BACKGROUND:Transcranial magneto-acoustic-electrical stimulation is a novel non-invasive neural regulation technique that utilizes the induced electric field generated by the coupling effect of ultrasound and static magnetic field to regulate the discharge activity of the nervous system.However,the mechanism by which it affects synaptic plasticity in the brain is still not enough. OBJECTIVE:To explore the effect of transcranial magneto-acoustic-electrical stimulation intensity on synaptic plasticity of the prefrontal cortex neural network in mice. METHODS:(1)Animal experiment:Twenty-four C57 mice were equally and randomly divided into four groups:the control group receiving pseudo-stimulation,the 6.35 W/cm2 stimulation group receiving coupled stimulation of 0.3 T,6.35 W/cm2,the 17.36 W/cm2 stimulation group receiving coupled stimulation of 0.3 T,17.36 W/cm2,and the 56.25 W/cm2 stimulation group receiving coupled stimulation of 0.3 T,56.25 W/cm2.The local field potential signals and behavioral correctness were recorded during the execution of T-maze in mice.(2)Modeling and simulation experiments:A neural network model of the prefrontal cortex in mice stimulated by transcranial magneto-acoustic-electrical stimulation was constructed to compare the structural connectivity characteristics of the neural network under different stimulation intensities. RESULTS AND CONCLUSION:Transcranial magneto-acoustic-electrical stimulation could effectively shorten the behavior learning time,improve the working memory ability of mice(P＜0.05),and continue to stimulate the frontal lobe of mice after learning behavior.There was no significant difference in the accuracy of the T-maze behavioral experiment among the experimental groups(P＞0.1).Analysis of local field potential signals in the frontal lobe of mice revealed that transcranial magneto-acoustic-electrical stimulation promoted energy enhancement of β and γ rhythms.As the stimulation intensity increased,there was an asynchronous decrease in β and γ rhythms.Through β-γ phase amplitude coupling,it was found that stimuli could enhance the neural network's ability to adapt to new information and task requirements.Modeling and simulation experiments found that stimulation could enhance the discharge level of the neural network,increase the long-term synaptic weight level,and decrease the short-term synaptic weight level only when the stimulation intensity was high.To conclude,there is a complex nonlinear relationship between different stimulus intensities and the functional structure of neural networks.This neural regulation technique may provide new possibilities for the treatment of related neurological diseases such as synaptic dysfunction and neural network abnormalities.

Objective To observe the pharmacokinetics and pharmacodynamics of doxorubicin hydrochloride liposomes combined with iodized oil in hepatic artery embolization of rabbit VX2 liver cancer.Methods Doxorubicin hydrochloride liposomes-lipiodol suspension were prepared at ratios of 1∶1,1∶2 and 2∶1,respectively,and the delamination time of each suspension was observed.Twenty New Zealand white rabbits were randomly divided into group A—D(each n=5).Doxorubicin hydrochloride liposomes-iodized oil emulsion was injected into group A,while doxorubicin hydrochloride-iodized oil emulsion was injected into group B for embolization of hepatic artery.Hepatic artery infusion was performed in group C with doxorubicin hydrochloride liposomes,while in group D with doxorubicin hydrochloride.The blood drug concentrations 1,4,8,12,16,20 and 24 h after treatments were compared among 4 groups.Another 20 healthy New Zealand white rabbits were modeling,and the rabbit models of VX2 liver cancer were randomly divided into experimental group and control group(each n=10).Doxorubicin hydrochloride liposomes-iodized oil emulsion was used in experimental group,while doxorubicin-iodized oil emulsion was used in control group for embolization of hepatic artery.The therapeutic effect was evaluated according to modified response evaluation criteria in solid tumors(mRECIST),and the objective response rate(ORR)and disease control rate(DCR)were recorded.Results Doxorubicin hydrochloride liposomes-lipiodol suspension with different proportions were all stratified within 1 min.Pharmacokinetic experiment showed that the blood drug concentration of group A and C were similar at each time point after treatment,and both higher than that in group B and D.Pharmacodynamics experiments showed that ORR and DCR in experimental group was 50.00%(5/10)and 90.00%(9/10),respectively,while in control group was 40.00%(4/10)and 80.00%(8/10),respectively.Conclusion Blood drug concentrations of doxorubicin hydrochloride were similar at each time point after hepatic artery embolization or hepatic artery infusion.Doxorubicin hydrochloride liposomes-iodized oil suspension had the same effect of doxorubicin-lipiodol emulsion for hepatic artery embolization of rabbit VX2 liver cancer.

Aiming at the problems of tongue diagnosis instruments relying on manual operation,uneven illumination,color distortion and lack of dynamic information,an automated operation black box tongue diagnosis instrument based on machine vision is proposed.This instrument utilizes machine vision technology and image processing algorithms to achieve automatic positioning and automatic acquisition of tongue manifestations.High-resolution camera technology enables the acquisition of 3-second dynamic videos and high-quality static images.The performance test results show that,based on the dual-camera geometric projection calibration algorithm,the average time for tongue recognition is less than 0.15 seconds.The diffuse reflection light source improves the gray uniformity to 76.74%,and the maximum color difference in color reproduction degree is 19,which is better than the industry standard value of 25.The developed tongue diagnosis instrument will promote the objective,efficient and precise acquisition of tongue diagnosis in traditional Chinese medicine.

Objective To observe the pharmacokinetics and pharmacodynamics of doxorubicin hydrochloride liposomes combined with iodized oil in hepatic artery embolization of rabbit VX2 liver cancer.Methods Doxorubicin hydrochloride liposomes-lipiodol suspension were prepared at ratios of 1∶1,1∶2 and 2∶1,respectively,and the delamination time of each suspension was observed.Twenty New Zealand white rabbits were randomly divided into group A—D(each n=5).Doxorubicin hydrochloride liposomes-iodized oil emulsion was injected into group A,while doxorubicin hydrochloride-iodized oil emulsion was injected into group B for embolization of hepatic artery.Hepatic artery infusion was performed in group C with doxorubicin hydrochloride liposomes,while in group D with doxorubicin hydrochloride.The blood drug concentrations 1,4,8,12,16,20 and 24 h after treatments were compared among 4 groups.Another 20 healthy New Zealand white rabbits were modeling,and the rabbit models of VX2 liver cancer were randomly divided into experimental group and control group(each n=10).Doxorubicin hydrochloride liposomes-iodized oil emulsion was used in experimental group,while doxorubicin-iodized oil emulsion was used in control group for embolization of hepatic artery.The therapeutic effect was evaluated according to modified response evaluation criteria in solid tumors(mRECIST),and the objective response rate(ORR)and disease control rate(DCR)were recorded.Results Doxorubicin hydrochloride liposomes-lipiodol suspension with different proportions were all stratified within 1 min.Pharmacokinetic experiment showed that the blood drug concentration of group A and C were similar at each time point after treatment,and both higher than that in group B and D.Pharmacodynamics experiments showed that ORR and DCR in experimental group was 50.00%(5/10)and 90.00%(9/10),respectively,while in control group was 40.00%(4/10)and 80.00%(8/10),respectively.Conclusion Blood drug concentrations of doxorubicin hydrochloride were similar at each time point after hepatic artery embolization or hepatic artery infusion.Doxorubicin hydrochloride liposomes-iodized oil suspension had the same effect of doxorubicin-lipiodol emulsion for hepatic artery embolization of rabbit VX2 liver cancer.

Objective:To investigate the effect and safety of the double-cross dressing technique for composite rhytidectomy.Methods:A retrospective analysis was performed on a total of 121 patients with face and neck ageing, who were admitted to the Facial and Neck Plastic Surgery Center, the Plastic Surgery Hospital of the Chinese Academy of Medical Sciences from June 2020 to May 2023, all female with the age ranged 34-72 years old. All the patients underwent a full facial and neck composite rhytidectomy. Immediately after surgery, a double-cross bandaging was applied. The cotton pad was nested on the outer auricle, so that the front of the ear and the back of the ear were the first cross. This cross aimed to accurately pressurized the temporal, buccal region and postauricle flap. The second cross occurred at the radix. It secured composite tissue flap at the zygomatic arch and the buccal fat pad zone. Patient′s external auricle was not compressed after bandaging. The healing and the occurrence of complications were observed.Results:All the patients were satisfied with the comfort of the bandaging, the satisfied rate was 100% (121/121). All the patients had different degrees of periocular and perioral swelling 72 hours after surgery. Conjunctival edema and xanthochromia occurred in 30 patients, and oral mucosal congestion occurred in 25 patients, all of which were spontaneously resolved 2-4 weeks after surgery. Postoperative nursing was easily managed. There were no events such as dressing loosening, blocked draining tubes, and difficulty in replacing tubes. All the patients were followed up for 3-12 months after surgery, the flap in the postauricular area of all patients healed well, and there were no adverse complications such as wound infection, necrosis of the flap in the operative area, and delayed healing of the incision.Conclusion:The double-cross bandaging technique achieves the comfort bandaging and good protection of the face, which is safe and effective.

Astrocytes and microglia engage in extensive and complex communication and mutual effect, which referrs to microglia-astrocyte crosstalk. Recent studies have highlighted that this crosstalk plays a pivotal role in neurodegenerative diseases, exerting either protective or detrimental effects. This review briefly introduces the molecular mechanism of microglia-astrocyte crosstalk and its research progress in Alzheimer's disease, multiple sclerosis, amyotrophic lateral sclerosis, and Parkinson's disease, aiming to provide new research directions and therapeutic targets for clinical improvement of neurodegenerative diseases from perspective of microglia-astrocyte crosstalk.