BACKGROUND:Poly(lactic-co-glycolic acid)copolymer with good biosafety,biodegradability,and superior mechanical properties has become a focal point of research in stomatology.OBJECTIVE:To review the advance in stomatology of poly(lactic-co-glycolic acid)copolymer.METHODS:Using a computer-assisted search of relevant literature published in the China National Knowledge Infrastructure(CNKI)and PubMed databases,the search terms included"polylactic acid-hydroxyacetic acid,PLGA,oral defect,tissue engineering"in Chinese,and"PLGA,polylactic acid-hydroxyacetic acid copolymer,poly(lactic-co-glycolic acid)copolymer,dent*,regeneration,caries,periodontal,pulp,implant,alveolar bone"in English.Preliminary screening was conducted by reading titles and abstracts,excluding literature unrelated to the theme of the article.Based on the inclusion and exclusion criteria,a total of 119 articles were included for review.RESULTS AND CONCLUSION:In the field of stomatology,the application scope of poly(lactic-co-glycolic acid)copolymer is rapidly expanding,gradually replacing the traditional therapeutic drugs and restorative materials.Poly(lactic-co-glycolic acid)copolymer nanoparticles/microspheres can carry a variety of hydrophobic and hydrophilic active substances,demonstrating excellent delivery capabilities in caries prevention,root canal disinfection,and pulp capping treatment.In periodontal therapy,poly(lactic-co-glycolic acid)copolymer is widely used as a barrier membrane and drug carrier for periodontal tissue regeneration.Poly(lactic-co-glycolic acid)copolymer used for surface modification of implants not only enhances the antibacterial ability of the implant surface but also improves the bio-inert nature of the implant surface.The effect of pure poly(lactic-co-glycolic acid)copolymer scaffolds on treating bone defects is limited and requires the integration of 3D printing,various bioactive components,and inorganic materials to enhance scaffold performance.The combination of poly(lactic-co-glycolic acid)copolymer and stem cells can improve the effectiveness of nerve therapy,meeting clinical needs.Based on the great potential of poly(lactic-co-glycolic acid)copolymer in the field of stomatology,it is expected that in the future,repair materials with specific functions for different diseases will be produced according to different needs of oral tissue engineering.

BACKGROUND:Poly(lactic-co-glycolic acid)copolymer with good biosafety,biodegradability,and superior mechanical properties has become a focal point of research in stomatology.OBJECTIVE:To review the advance in stomatology of poly(lactic-co-glycolic acid)copolymer.METHODS:Using a computer-assisted search of relevant literature published in the China National Knowledge Infrastructure(CNKI)and PubMed databases,the search terms included"polylactic acid-hydroxyacetic acid,PLGA,oral defect,tissue engineering"in Chinese,and"PLGA,polylactic acid-hydroxyacetic acid copolymer,poly(lactic-co-glycolic acid)copolymer,dent*,regeneration,caries,periodontal,pulp,implant,alveolar bone"in English.Preliminary screening was conducted by reading titles and abstracts,excluding literature unrelated to the theme of the article.Based on the inclusion and exclusion criteria,a total of 119 articles were included for review.RESULTS AND CONCLUSION:In the field of stomatology,the application scope of poly(lactic-co-glycolic acid)copolymer is rapidly expanding,gradually replacing the traditional therapeutic drugs and restorative materials.Poly(lactic-co-glycolic acid)copolymer nanoparticles/microspheres can carry a variety of hydrophobic and hydrophilic active substances,demonstrating excellent delivery capabilities in caries prevention,root canal disinfection,and pulp capping treatment.In periodontal therapy,poly(lactic-co-glycolic acid)copolymer is widely used as a barrier membrane and drug carrier for periodontal tissue regeneration.Poly(lactic-co-glycolic acid)copolymer used for surface modification of implants not only enhances the antibacterial ability of the implant surface but also improves the bio-inert nature of the implant surface.The effect of pure poly(lactic-co-glycolic acid)copolymer scaffolds on treating bone defects is limited and requires the integration of 3D printing,various bioactive components,and inorganic materials to enhance scaffold performance.The combination of poly(lactic-co-glycolic acid)copolymer and stem cells can improve the effectiveness of nerve therapy,meeting clinical needs.Based on the great potential of poly(lactic-co-glycolic acid)copolymer in the field of stomatology,it is expected that in the future,repair materials with specific functions for different diseases will be produced according to different needs of oral tissue engineering.

ObjectiveTo investigate the radioactive level of gross alpha and beta in drinking water in one district in Shanghai and provide a scientific basis for measuring the level of radiation in case of drinking water pollution due to potential nuclear accidents. MethodsA total of154 samples collected across the district were monitored by using the standard examination method for drinking water - radiological parameters GB/T 5750.13-2006. ResultsAll the samples of the drinking water conformed to the standard for drinking water quality GB 5749-2006. The differences between different seasons were significant. The difference of gross alpha and beta radioactivity of drinking water was significant between the high water period and the dry water period. The former was higher than the latter. ConclusionIt is very important to monitor and study radioactivity of drinking water regularly for the prevention and control of health damage caused by radioactive water pollution.

Objective To investigate the effect and potential mechanism of microRNA (miRNA)-377 on high glucose-induced proliferation and inflammation in human mesangial cells.Methods Cells were randomly divided into six groups:control group (5.5 mmol/L glucose),high glucose group (30.0 mmol/L glucose),negtive miRNA inhibitor transfection+high glucose group,negtive miRNA mimic transfection+high glucose group,miRNA-377 inhibitor transfection+high glucose group (miR-377i+high glucose group),miRNA-377 mimic transfection+high glucose group (miR-377m+high glucose group),miRNA-377 expression was detected by real-time PCR.Cell proliferation and cell cycle were detected by BrdU assay and flow cytometry,respectively.The release of tumor necrosis factor-α (TNF-α),interleukin (IL)-18,IL-6 and macrophages chemotaxis protein-1 (MCP-1) were evaluated by ELISA.The activations of NF-κB pathway,including the expressions of phosporylated (p)-IκBα,p-P65 and nuclear P65,were measured by Western blotting.Results Compared with those in control group,in high glucose group cell viability,miRNA-377 expression and cell proliferation rate increased (all P ＜ 0.05),proportions of S phase cell and G2/M phase cell in cell cycle increased (all P ＜ 0.05),the levels of TNF-o,IL-18,IL-6 and MCP-1 were higher (all P ＜ 0.05),as well as the expressions of p-IKBα/IκBα,p-P65/P65 and nuclear P65 were increased (all P ＜ 0.05).Compared with high glucose group,cell proliferation rate was restrained (P ＜ 0.05),proportions of S phase cell and G2/M phase cell in cell cycle was descreased (all P ＜ 0.05),the levels of TNF-α,IL-18,IL-6 and MCP-1 were lower (all P ＜ 0.05),as well as the expressions of p-IκBα/IκBα,p-P65/P65 and nuclear P65 were reduced (all P ＜ 0.05) in miR-377i+high glucose group.However,miR-377m+high glucose group presented opposite results (all P ＜ 0.05).Conclusions miRNA-377 knockdown can partially suppress high glucose-induced human mesangial cell proliferation and cell cycle transition,and restrain inflammatory molecules release.Its mechanism may be related to the inhibition of NF-κB pathway.