Objective:To analyze the epidemiological characteristics and trend of hospitalization of the patients with herpes zoster in Beijing from 2017 to 2022.Methods:In this retrospective study, the information of hospitalization of herpes zoster patients were collected from all medical institutions at the first level and above in Xicheng, Changping, and Miyun districts of Beijing. The age and gender specific hospitalization rates and age-standardized hospitalization rates were calculated. Joinpoint regression model was used to explore the trend of the hospitalization rates, and the influencing factors of the hospital stay length and complications were analyzed.Results:The age-standardized hospitalization rate of the patients with herpes zoster was 10.82/100 000-18.43/100 000 in Beijing from 2017 to 2022 [annual percent change (APC) =5.86%, 95% CI: -2.80%-15.98%]. The age-standardized hospitalization rate of the cases with herpes zoster as the main diagnosis showed an upward trend (APC=11.35%, 95% CI: 7.21%-16.23%). The age-standardized hospitalization rate showed an upward trend in women (APC=14.34%, 95% CI: 7.95%-22.37%). The hospitalization rate showed a downward trend in age group 30-39 years (APC=-24.92%, 95% CI: -48.56% - -1.85%) and showed upward trends in age group 70-79 years and 80-109 years (APC=23.18%, 95% CI: 13.53%-35.58%; APC=4.90%, 95% CI: 1.18%-9.19%). Complications occurred in 66.28% (680/1 026) of the patients. The median hospital stay length was 9 (5,15) days, and the patients with high age (≥80 years) and two or more complications had longer hospital stay, which were 12 (6, 23) and 14 (7, 27) days respectively ( P<0.001). Conclusions:The hospitalization rate in women and the elderly aged ≥70 years with herpes zoster as the main diagnosis showed upward trends in Beijing in recent years. The elderly aged ≥80 years usually had longer hospital stay, showing a relatively disease burden level. More attention should be paid to development of intervention strategies, such as vaccine, for this population.

Objective:To understand the incidence rate of herpes zoster (HZ) and postherpetic neuralgia (PHN) in Beijing, and analyze the incidence trend of HZ and PHN from 2015 to 2022.Methods:Cases of HZ and PHN from 2015 to 2022 were retrieved from the Hospital Information Systems (HIS) of all primary and above hospitals/clinics in three districts representing the urban, inner suburban, and outer suburban areas of Beijing. After duplication screening, the first visit cases were screened, and the incidence characteristics were described. The incidence rate of HZ and PHN in each year by sex and age group and the age-standardized incidence rate were calculated. The annual percentage increase (APC) of incidence rate was calculated using the Joint regression model, and the change trend was analyzed.Results:The age-standardized incidence rate of HZ in Beijing from 2015 to 2022 ranged from 7.44‰ to 10.05‰, with an average annual incidence rate of 8.95 ‰, significantly increasing with age ( P<0.001). The Joinpoint regression model showed that the overall age-standardized incidence of HZ remained relatively stable, with no significant difference (APC=2.28%, t=1.56, P=0.170). However, the incidence rate among the 0-19-year-old group exhibited a trend of decrease (APC=-10.70%, t=-6.29, P<0.001). For PHN, the age-standardized incidence in Beijing ranged from 0.77‰ to 2.67‰, with an average annual incidence rate of 1.59‰ and a proportion of 9.48% to 26.86% among HZ cases. Both the incidence of PHN and its proportion among HZ cases increased with age ( P<0.001). The age-standardized incidence of PHN increased annually (APC=18.56%, t=9.02, P<0.001). Conclusion:The incidence rate of HZ and PHN in Beijing continues to be at a high level, and PHN shows an increasing trend over time.

Objective To systematically evaluate the current status of quality of life in infertile patients and its influencing factors.Methods Computer search of PubMed,Cochrane Library,Web of Science,Embase,CBM,Knowledge Network,Wanfang,and Wipu,with a search timeframe from the establishment of the library to December 2023,and meta-analysis using CMA 3.3 software.Results A total of 33 literatures were included in the current status of quality of life,which showed that the level of quality of life was 60.841[95％CI(58.029,63.654),P＜0.001]for the FertiQoL scale and 67.630[95％CI(59.541,75.718),P＜0.001]for the SF-36 scale.A total of 45 papers were included in the literature on factors influencing quality of life,and the results showed that personal trait factors of young age(r=0.181,P=0.006)and history of pregnancy(r=0.258,P＜0.001)were lowly positively correlated with quality of life,and that long years of infertility(r=-0.267,P＜0.001),a history of treatment failure(r=-0.292,P＜0.001),and long duration of treatment(r=-0.213,P＜0.001)had a low negative association with quality of life.Behavioural factors such as anxiety(r=-0.547,P＜0.001),depression(r=-0.556,P＜0.001),and stigma(r=-0.530,P＜0.001)were highly negatively correlated with the quality of life;high fertility stress(r=-0.333,P=0.010)was moderately negatively correlated with the quality of life;and good psychological resilience(r=0.383,P＜0.001)and high self-esteem(r=0.343,P=0.001)were moderately positively associated with quality of life.Interpersonal network factors of good relationship with husband(r=0.337,P＜0.001)were moderately positively correlated with quality of life,and having a reproductive history(r=0.124,P＜0.001)was lowly positively correlated with quality of life.Good occupation(r=0.149,P=0.002)was low positively associated with quality of life among the factors of work and living conditions;high annual household income(r=0.307,P＜0.001)was moderately positively associated with quality of life.Having health insurance in the policy environment(r=0.131,P＜0.001)was low positively associated with quality of life.Conclusion The level of quality of life of infertile patients is impaired to some extent and there are many influencing factors,so healthcare professionals should comprehensively assess the quality of life of patients and adopt targeted and personalised interventions to improve the quality of life of patients.

Objective To systematically evaluate the current status of quality of life in infertile patients and its influencing factors.Methods Computer search of PubMed,Cochrane Library,Web of Science,Embase,CBM,Knowledge Network,Wanfang,and Wipu,with a search timeframe from the establishment of the library to December 2023,and meta-analysis using CMA 3.3 software.Results A total of 33 literatures were included in the current status of quality of life,which showed that the level of quality of life was 60.841[95％CI(58.029,63.654),P＜0.001]for the FertiQoL scale and 67.630[95％CI(59.541,75.718),P＜0.001]for the SF-36 scale.A total of 45 papers were included in the literature on factors influencing quality of life,and the results showed that personal trait factors of young age(r=0.181,P=0.006)and history of pregnancy(r=0.258,P＜0.001)were lowly positively correlated with quality of life,and that long years of infertility(r=-0.267,P＜0.001),a history of treatment failure(r=-0.292,P＜0.001),and long duration of treatment(r=-0.213,P＜0.001)had a low negative association with quality of life.Behavioural factors such as anxiety(r=-0.547,P＜0.001),depression(r=-0.556,P＜0.001),and stigma(r=-0.530,P＜0.001)were highly negatively correlated with the quality of life;high fertility stress(r=-0.333,P=0.010)was moderately negatively correlated with the quality of life;and good psychological resilience(r=0.383,P＜0.001)and high self-esteem(r=0.343,P=0.001)were moderately positively associated with quality of life.Interpersonal network factors of good relationship with husband(r=0.337,P＜0.001)were moderately positively correlated with quality of life,and having a reproductive history(r=0.124,P＜0.001)was lowly positively correlated with quality of life.Good occupation(r=0.149,P=0.002)was low positively associated with quality of life among the factors of work and living conditions;high annual household income(r=0.307,P＜0.001)was moderately positively associated with quality of life.Having health insurance in the policy environment(r=0.131,P＜0.001)was low positively associated with quality of life.Conclusion The level of quality of life of infertile patients is impaired to some extent and there are many influencing factors,so healthcare professionals should comprehensively assess the quality of life of patients and adopt targeted and personalised interventions to improve the quality of life of patients.

Objective The purpose of the study is to breed homozygous interferon-γ knockout(IFN-γ-/-)mice and optimize the breeding strategies to achieve continuous and stable reproduction of IFN-γ-/-mice,which could be used as an ideal animal model for fundamental research.Methods Initially,heterozygous IFN-γ knockout(IFN-γ+/-)C57BL/6J mice were used as the parental generation for breeding.Subsequently,3 breeding strategies were employed using the offspring:(1)female heterozygotes mated with male heterozygotes;(2)male homozygotes mated with female heterozygotes;(3)female homozygotes mated with male homozygotes.The number and survival rate of IFN-γ-/-mice were compared across the three breeding strategies to determine the optimal breeding strategy.Under the optimal strategy,the effects of female mating age and diet type on the reproductive performance of IFN-γ-/-mice were further evaluated.Data from the first three litters of 60 IFN-γ-/-female mice,including litter size,number of weaning survivors,and weaning survival rate,were recorded and analyzed.In addition,the effects of dietary supplementation of pregnant mice and environmental optimization measures,such as the provision of shelters,were evaluated.Results Under conditions where the nutritional needs of pregnant mice were adequately met by supplementation with egg yolk and sunflower seeds,mating of female and male IFN-γ-/-mice result ed in a litter size of five to eight IFN-γ-/-mice,demonstrating higher efficiency compared to other breeding strategies.In addition,diet type and mating age significantly influenced female reproductive performance.When 7～9 weeks old female IFN-γ-/-mice were mated to male IFN-γ-/-mice and fed a high-protein breeding diet,litter size(6.9±1.7),weaning survival number(6.5％±2.0％)and weaning survival rate(93.2％±17.8％)were higher than those under other conditions.In addition,providing shelters to prevent fighting between breeding pairs further improved reproductive outcomes.Conclusions By adopting an optimized breeding strategy,combined with a high-protein diet,nutritional supplementation,and standardized mating age management,the breeding efficiency and stability of IFN-γ-/-mice can be significantly improved.This provides a reliable animal model for related research.

Objective To investigate the clinical application value of detection of interleukin-6(IL-6),C reactive protein(CRP),and neutrophic-to-lymphocyte ratio(NLR)alone and in combination in the prognosis assessment of severe pneumonia(SP).Methods A total of 81 patients with SP admitted from December 2022 to December 2023 were selected as the research subjects.According to the discharge outcomes of the patients,they were divided into the survival group(n=62)and the death group(n=19).The general clinical data at admission and laboratory parameters of the two groups were collected and compared.The receiver operating characteristic(ROC)curve was used to analyze the value of serum IL-6,CRP and NLR detection in the prognosis assessment of SP.Results At admission,the pulse of the death group was higher than that of the survival group,while the systolic blood pressure(SBP)was lower than that of the survival group(P<0.05,P<0.01).The incidence of chest tightness in the death group at admission was significant higher than that in the survival group(P<0.05).Compared with the survival group,the number of lymphocyte counts and albumin levels in the death group were lower,while the percentage of neutrophils,NLR and procalcitonin were higher than those in the survival group(P<0.05,P<0.01).ROC curve analysis showed that the area under the curve(AUC)for predicting poor prognosis of SP by the combined detection of IL-6,CRP and NLR was 0.75,which was higher than that predicted by each indicator alone.Conclusion Compared with the detection of IL-6,CRP and NLR alone,the combined detection of the three has a more significant value in predicting the risk of death in patients with SP.It suggests that clinicians should promptly,comprehensively and dynamically monitor the above infection indicators during diagnosis and treatment,and take prompt and effective intervention measures to reduce the mortality rate of patients.

Objective:To investigate the predictive value of nutritional risk related indicators in the prognosis of elderly esophageal squamous cell carcinoma (ESCC) patients undergoing radical radiotherapy.Methods:A retrospective case series study was conducted. The clinical data of ESCC patients aged ≥ 70 years who received radical radiotherapy in Shanxi Province Cancer Hospital from January 2015 to December 2019 were retrospectively analyzed. According to the radiotherapy planning system, the maximum transverse diameter of gross tumor volume of primary tumors (GTVt) and GTVt volume in the esophagus were calculated. The nutritional risk related indicators of ESCC patients before and after radiotherapy were calculated and recorded, including body mass index (BMI), geriatric nutritional risk index (GNRI), and neutrophil-to-lymphocyte ratio (NLR); Kaplan-Meier method was used for survival analysis, and log- rank test was performed; Cox proportional risk model was used to analyze the factors influencing the prognosis of patients. The overall survival (OS), progression-free survival (PFS), short-term efficacy, and adverse reactions of patients with different clinicopathological characteristics were compared.Results:The last follow-up time was 30 December, 2023. The 1-year, 3-year, and 5-year OS rates of 161 elderly patients with ESCC were 79.5%, 33.9%, and 16.1% respectively, with a median OS time of 25.8 months (95% CI: 20.11-31.49 months); the 1-year, 3-year, and 5-year PFS rates were 65.8%, 28.9%, and 14.8%, respectively, with a median PFS time of 20.0 months (95% CI: 16.31-23.69 months). There were statistically significant differences in the median OS time of patients with different age, lesion contrast length, maximum transverse diameter of GTVt, GTVt volume, short-term efficacy, BMI before radiotherapy, BMI after radiotherapy, GNRI after radiotherapy, NLR before radiotherapy, and NLR after radiotherapy (all P < 0.05); there were statistically significant differences in the median PFS time of ESCC patients with different age, maximum transverse diameter of GTVt, GTVt volume, short-term efficacy, BMI before radiotherapy, BMI after radiotherapy, GNRI after radiotherapy, and NLR before radiotherapy (all P <0.05). The results of multivariate Cox regression analysis showed that the maximum transverse diameter of GTVt was an independent influencing factor of the patients' OS ( P < 0.05); GTVt volume, short-term efficacy, GNRI after radiotherapy, and NLR before radiotherapy were independent influencing factors for OS and PFS of the patients (all P < 0.05). Among 161 elderly patients with ESCC, 45 achieved complete remission (CR), 111 achieved partial remission (PR), and 5 achieved stable disease (SD). There were statistically significant differences in the proportions of patients with different maximum transverse diameter of GTVt, GTVt volume, BMI after radiotherapy, GNRI after radiotherapy, and NLR before radiotherapy reaching CR and PR+SD (all P < 0.05). Among the 161 patients, grade 0, 1, 2, and 3 radiation-induced esophagitis (RE) occurred in 59, 54, 42, and 6 cases, respectively during treatment and within 3 months after treatment; among them, ≥ grade 2 RE occurred in 48 cases (29.8%); grade 0, 1, 2, and 3 radiation pneumonitis (RP) occurred in 95, 38, 25, and 3 cases, respectively; among them, ≥ grade 2 RP occurred in 28 cases (17.4%). Conclusions:Nutritional risk related indexes GNRI and NLR may be predictive indicators for the prognosis of elderly ESCC patients undergoing radical radiotherapy.

Objective:To analyze the epidemiological characteristics and trend of hospitalization of the patients with herpes zoster in Beijing from 2017 to 2022.Methods:In this retrospective study, the information of hospitalization of herpes zoster patients were collected from all medical institutions at the first level and above in Xicheng, Changping, and Miyun districts of Beijing. The age and gender specific hospitalization rates and age-standardized hospitalization rates were calculated. Joinpoint regression model was used to explore the trend of the hospitalization rates, and the influencing factors of the hospital stay length and complications were analyzed.Results:The age-standardized hospitalization rate of the patients with herpes zoster was 10.82/100 000-18.43/100 000 in Beijing from 2017 to 2022 [annual percent change (APC) =5.86%, 95% CI: -2.80%-15.98%]. The age-standardized hospitalization rate of the cases with herpes zoster as the main diagnosis showed an upward trend (APC=11.35%, 95% CI: 7.21%-16.23%). The age-standardized hospitalization rate showed an upward trend in women (APC=14.34%, 95% CI: 7.95%-22.37%). The hospitalization rate showed a downward trend in age group 30-39 years (APC=-24.92%, 95% CI: -48.56% - -1.85%) and showed upward trends in age group 70-79 years and 80-109 years (APC=23.18%, 95% CI: 13.53%-35.58%; APC=4.90%, 95% CI: 1.18%-9.19%). Complications occurred in 66.28% (680/1 026) of the patients. The median hospital stay length was 9 (5,15) days, and the patients with high age (≥80 years) and two or more complications had longer hospital stay, which were 12 (6, 23) and 14 (7, 27) days respectively ( P<0.001). Conclusions:The hospitalization rate in women and the elderly aged ≥70 years with herpes zoster as the main diagnosis showed upward trends in Beijing in recent years. The elderly aged ≥80 years usually had longer hospital stay, showing a relatively disease burden level. More attention should be paid to development of intervention strategies, such as vaccine, for this population.

Objective To investigate the pharmacodynamic material basis of Bawei Chenxiang Powder in the treatment of coronary heart disease.Methods The action targets of 16 blood-entry components of Bawei Chenxiang Powder were retrieved by TCMSP,PharmMapper and SwissTargetPrediction,and the disease targets were found by GeneCards and OMIM databases,which screened the intersection targets of the components and the disease;a component-target network was constructed using Cytoscape 3.9.1 software and key components were screened;the protein-protein interaction network was obtained by using STRING database and core targets were screened;GO and KEGG pathway enrichment analysis of intersecting targets was performed using DAVID database;molecular docking between key components and core targets was performed;molecular dynamics simulations were performed using Amber 20 software;UPLC-Q-Exactive hybrid quadrupole-Orbitrap mass spectrometry was used to quantify the key components in rat plasma;the hypoxia/reoxygenation model of H9c2 cells was constructed to investigate the effects of the key components on the survival rate and LDH of H9c2 cells.Results Totally 514 targets of the 16 blood-entry components of Bawei Chenxiang Powder,1716 targets of coronary heart disease,and 192 intersecting targets were retrieved,among which the key components were quercetin,kaempferol,naringenin,dehydrodiisoeugenol,and the core targets were ALB,IL6,TNF,GAPDH,AKT1,IL1B and TP53;the KEGG results showed that the mechanism of Bawei Chenxiang Powder for treating coronary heart disease may be related to PI3K-Akt signaling pathway,MAPK signaling pathway,HIF-1 signaling pathway,IL-17 signaling pathway,etc.;molecular docking showed that the key components had low binding energy with the core targets;molecular dynamics simulation results showed that the binding energy of TNF and quercetin was tight and stable,with a binding energy of-20.263 3 kcal/mol;the concentrations of the key components in plasma were quercetin(15.65±1.76)ng/mL,kaempferol(10.58±0.96)ng/mL,naringenin(3.07±0.69)ng/mL,and dehydrodiisoeugenol(4.90±1.18)ng/mL;the results of cellular experiments showed that the above four components could significantly increase the survival rate of H9c2 cells under hypoxia/reoxygenation conditions and reduce the LDH activity in the cell supernatant.Conclusion Quercetin,kaempferol,naringenin,dehydrodiisoeugenol and other components in Bawei Chenxiang Powder may be the pharmacodynamic material basis for treating coronary heart disease.

Objective:To construct a questionnaire to measure parents′ behavioral and social drivers (BeSD) in administering rotavirus vaccine to their children, and to test the reliability and validity of the questionnaire.Methods:A questionnaire was constructed to investigate the BeSD of rotavirus vaccination among parents based on the WHO BeSD questionnaire. A survey was conducted among parents of children aged 0-3 years old in vaccination clinics from 15 community health service centers in Nanjing City of Jiangsu Province, Yuhuan and Kaihua Counties of Zhejiang Province, Anqing City of Anhui Province, and Yangpu District of Shanghai City from April to May in 2024. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were used to analyze the reliability (internal consistency reliability, combination reliability and split half reliability) and validity (structural validity, convergence validity and discriminative validity) of the questionnaire.Results:A total of 850 questionnaires were collected, of which 614 were valid, with an effective response rate of 72.20%. The EFA resulted in the refinement of the rotavirus vaccine BeSD questionnaire from 37 to 35 items (including 24 core items), and suggested a five-factor structure including the perceptions of vaccine, confidence in vaccine, social process of vaccination, motivation for vaccination, and practical problems of vaccination. The cumulative variance contribution rate reached 56.27%. The CFA confirmed an ideal five-factor model fit (GFI=0.82,CFI=0.86,PNFI=0.67,RMSEA=0.07). The AVE of each dimension was greater than 0.50. The AVE square root of each dimension of the questionnaire was greater than its correlation coefficient. The Cronbach′s α coefficient of the total questionnaire was 0.79.Conclusion:The developed BeSD questionnaire of rotavirus vaccine has good reliability and validity, and can be used to measure parents′ behavioral and social factors in administering rotavirus vaccine to their children.