1.Effectiveness of an online patient education video for transcatheter aortic valve implantation.
Samuel Ji Quan KOH ; Jonathan YAP ; Chun Yen KOK ; Yilin JIANG ; Yu Jen LOO ; Michelle Wei Ling HO ; Yu Fei LIM ; See Hooi EWE ; Mohammed Rizwan AMANULLAH ; Zameer Abdul AZIZ ; Sivaraj GOVINDASAMY ; Victor CHAO ; Kay Woon HO
Annals of the Academy of Medicine, Singapore 2025;54(3):197-199
2.Transfected human mesenchymal stem cells do not lose their surface markers and differentiation properties.
Yap, Fei-Ling ; Cheong, Soon-Keng ; Ammu, Radhakrishnan ; Leong, Chooi-Fun
The Malaysian Journal of Pathology 2009;31(2):113-20
In this study, we evaluated the biological properties of human mesenchymal stem cells transfected (hMSC) with a plasmid vector expressing human cytokine interleukin-12 (IL-12). Surface markers were analysed by immunophenotyping using flow cytometry. Differentiation capability was evaluated towards adipogenesis and osteogenesis. We demonstrated that successfully transfected hMSC retained their surface immunophenotypes and differentiation potential into adipocytes and osteocytes. These results indicate that hMSC may be a suitable vehicle for gene transduction.
Antigens, Surface/metabolism
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Biological Markers/metabolism
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Bone Marrow Cells/cytology
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Bone Marrow Cells/metabolism
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Cell Differentiation/physiology
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Cells, Cultured
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Flow Cytometry
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Immunophenotyping
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Interleukin-12/genetics
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Interleukin-12/metabolism
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Mesenchymal Stem Cells/*cytology
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Mesenchymal Stem Cells/metabolism
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Transfection
Result Analysis
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