Objective:To analyze the predictive value of combined detection of serum tumor abnormal protein (TAP), cytokeratin 19 fragment antigen (CYFRA21-1), and endothelial cell specific molecule 1 (ESM1) for postoperative recurrence and metastasis of colorectal cancer patients.Methods:Seventy-five patients with colorectal cancer(colorectal cancer group) admitted to Jinhua Guangfu Cancer Hospital from June 2021 to May 2023 and 60 healthy subjects (control group) during the same period were retrospectively selected as the study objects. The levels of serum TAP, CYFRA21-1 and ESM1 were compared between the two groups. The levels of serum TAP, CYFRA21-1 and ESM1 in colorectal cancer patients with different clinicopathological features were compared. All patients with colorectal cancer were followed up for 1 year, and 5 cases were lost to follow-up, including 40 cases in the non-recurrence and metastasis group and 35 cases in the recurrence and metastasis group. The levels of serum TAP, CYFRA21-1, and ESM1 were compared between the two groups. The receiver operating characteristic (ROC) curve was used to analyze the predictive value of three indexes for postoperative recurrence and metastasis of colorectal cancer.Results:The levels of serum TAP, CYFRA21-1 and ESM1 in the colorectal cancer group were higher than those in the control group: (134.52 ± 26.18) μm 2 vs. (95.52 ± 10.03) μm 2, (6.96 ± 0.73) μg/L vs. (3.15 ± 0.42) μg/L, (36.78 ± 4.97) μg/L vs. (421 ± 0.35) μg/L, there were statistical differences ( P<0.01). There were significant differences in serum TAP, CYFRA21-1, and ESM1 levels among patients with different clinical stages, tissue differentiation, and lymph node metastasis ( P<0.01). The levels of serum TAP, CYFRA21-1 and ESM1 in the recurrence and metastasis group were higher than those in the non-recurrence and metastasis group: (155.25 ± 28.02) μm 2 vs. (121.05 ± 21.03) μm 2, (8.58 ± 0.93) μg/L vs. (5.25 ± 0.62) μg/L, (42.05 ± 5.23) μg/L vs. (34.62 ± 5.01) μg/L, there were statistical differences ( P<0.01). ROC curve analysis results showed that the combined detection of serum TAP, CYFRA21-1 and ESM1 levels predicted postoperative recurrence and metastasis of colorectal cancer patients with the largest of the area under the curve (AUC), which was 0.811, and was higher than any single index. Conclusions:The levels of TAP, CYFRA21-1 and ESM1 are increased in colorectal cancer patients, and combined detection of serum TAP, CYFRA21-1 and ESM1 levels has high predictive value for postoperative recurrence and metastasis.

Objective:To analyze the predictive value of combined detection of serum tumor abnormal protein (TAP), cytokeratin 19 fragment antigen (CYFRA21-1), and endothelial cell specific molecule 1 (ESM1) for postoperative recurrence and metastasis of colorectal cancer patients.Methods:Seventy-five patients with colorectal cancer(colorectal cancer group) admitted to Jinhua Guangfu Cancer Hospital from June 2021 to May 2023 and 60 healthy subjects (control group) during the same period were retrospectively selected as the study objects. The levels of serum TAP, CYFRA21-1 and ESM1 were compared between the two groups. The levels of serum TAP, CYFRA21-1 and ESM1 in colorectal cancer patients with different clinicopathological features were compared. All patients with colorectal cancer were followed up for 1 year, and 5 cases were lost to follow-up, including 40 cases in the non-recurrence and metastasis group and 35 cases in the recurrence and metastasis group. The levels of serum TAP, CYFRA21-1, and ESM1 were compared between the two groups. The receiver operating characteristic (ROC) curve was used to analyze the predictive value of three indexes for postoperative recurrence and metastasis of colorectal cancer.Results:The levels of serum TAP, CYFRA21-1 and ESM1 in the colorectal cancer group were higher than those in the control group: (134.52 ± 26.18) μm 2 vs. (95.52 ± 10.03) μm 2, (6.96 ± 0.73) μg/L vs. (3.15 ± 0.42) μg/L, (36.78 ± 4.97) μg/L vs. (421 ± 0.35) μg/L, there were statistical differences ( P<0.01). There were significant differences in serum TAP, CYFRA21-1, and ESM1 levels among patients with different clinical stages, tissue differentiation, and lymph node metastasis ( P<0.01). The levels of serum TAP, CYFRA21-1 and ESM1 in the recurrence and metastasis group were higher than those in the non-recurrence and metastasis group: (155.25 ± 28.02) μm 2 vs. (121.05 ± 21.03) μm 2, (8.58 ± 0.93) μg/L vs. (5.25 ± 0.62) μg/L, (42.05 ± 5.23) μg/L vs. (34.62 ± 5.01) μg/L, there were statistical differences ( P<0.01). ROC curve analysis results showed that the combined detection of serum TAP, CYFRA21-1 and ESM1 levels predicted postoperative recurrence and metastasis of colorectal cancer patients with the largest of the area under the curve (AUC), which was 0.811, and was higher than any single index. Conclusions:The levels of TAP, CYFRA21-1 and ESM1 are increased in colorectal cancer patients, and combined detection of serum TAP, CYFRA21-1 and ESM1 levels has high predictive value for postoperative recurrence and metastasis.

Objective To explore the correlation between the occupational coping self-efficacy and family-work enrichment. Methods In June 2017, totally 58 male nurses were recruited by convenient sampling method and investigated by the Occupational Coping Self-efficacy Scale for nurses and Work-family Enrichment Questionnaire. Pearson correlation analysis and hierarchical regression analysis were used to analyze the effect of family-work enrichment on male nurses' professional coping self-efficacy. Results The mean scores of male nurses' occupational coping self-efficacy and work-family enrichment were (32.81±4.15) and (21.50±3.73) respectively. Pearson correlation analysis showed that each dimension of work-family enrichment was significantly correlated to occupational coping self-efficacy (r=0.592,P< 0.001). Hierarchical regression analysis showed that all the dimensions of work-family enrichment could explain 33.5% of the variance of male nurses' occupational coping self-efficacy. Conclusions Male nurses' occupational coping self-efficacy is closely related to family-work enrichment.

BACKGROUND: CpG oligodeoxynucleotide (ODN) is a type of highly effective immune adjuvant with low toxicity, which has an extensive application in gene therapy for many diseases. However, the specificity for species and cells leading to low uptake by cells and degradation by nuclease blocks its clinical application. OBJECTIVE: To explore the specific delivery and its immunologic efficacy of CpG ODN targeting B lymphocytes of umbilical cord blood by CD40 ligand-receptor-mediated carrier system. DESIGN, TIME AND SETTING: An observation and control experiment was performed at the Department of Hematology, and Department of Pediatric, the First Affiliated Hospital of Jinan University from April 2004 to October 2007. MATERIALS: Fresh umbilical cord blood with heparin was obtained from healthy, natal infant. Informed consent was obtained from his parents, and the experiment was approved by the hospital Ethics Committee. METHODS: CD40 ligand (CD40L)-EDC-PLL-CpG ODN conjugated complex was prepared. Mononuclear cells (MNCs) from umbilical cord blood were co-cultured with conjugated complexes. Uptake rate, mean fluorescence intensity of FAM marked CpG ODN, expressions of MNCs, proliferations of lymphocytes and the IgG levels of culture supematants were detected by flow cytometry, fluorescence techniques, MTT assay and ELISA, respectively. MAIN OUTCOME MEASURES: The uptake rate, the mean fluorescence intensity of CpG ODN by MNCs, subgroups and proliferations of lymphocytes, and IgG levels of culture supematants. RESULTS: Compared to the pure CpG ODN group, the uptake rate of the conjugated complexes group was higher (98%), the peak level of up-taking occurred earlier, and intracellular fluorescence intensity maintained much more stable. Expressions of CD19+, CD22+, and CD20+ was increased, A value and IgG levels in supematants were all higher than that of the control group. CONCLUSION: CD40 tigand-receptor-mediated carrier system is helpful for CpG ODN delivery targeting to B lymphocyte, enhancing its immunological efficiency.

AIM To investigate the effect of recipient kidney function by CsA coadministration Ber used to induce immune tolerance in rats of allogenic cardiac transplantation. METHODS The authors established the SD to Wistar rats heterotopic cardiac transplantation model by Onos methods.Observe the cardiac allograft survival and levels of BUN and Cr in the recipients plasma. The recipients were classified into 5 groups randomly after heterotopic cardiac transplantation were performed. Group A (Wistar to Wistar)): Received placebo intraperitoneal injected for 21 days; Group B (SD to Wistar): Saline intraperitoneal injected for 21 days; Group C (SD to Wistar):CsA 2 mg?kg -1 ?d -1 intraperitoneal injected for 21 days; Group D(SD to Wistar):Ber 16 mg?kg -1 ?d -1 gastrointubation for 21 days; Group E(SD to Wistar): Ber 16 mg?kg -1 ?d -1 gastrointubation coadministration CsA 2 mg?kg -1 ?d -1 ip for 21 days. RESULTS The levels of BUN and Cr in recipint plasma is lower evidently compare with the group with CsA ip simply. CONCLUSION Ber can reduce the renal toxicity in recipients by CsA which was intraperitoneal injected (ip) over a long period time.

AIM: To investigate the relationship between CD200~+CK7~+ trophoblasts and the resorption of embryos in a poly (I∶C)-induced abortion model. METHODS: The status of CD200 expression was investigated in Balb/c?C57BL/6 and Balb/c?Balb/c mice as induced model of embryo-resorption by an i.p. injection of poly (I∶C). CD200 expression on CK7~+ cells from placentas was detected with flow cytometry. CD200~+ cells in placenta were observed with immunocytochemical staining. RESULTS: Both the percentage and absolute number of CD200~+CK7~+ cells were dramatically decreased by injection of poly (I∶C) in Balb/c?C57BL/6 (6.3%?6.2% vs 36.1%?9.3%, P

AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35 37% at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage.

98%), the peak level of uptake occurred earlier, intracellular fluorescence intensity maintained much more stable. Expressions of CD19+, CD22+, CD20+ increased significantly. A_~570 values of MNCs proliferation and IgG levels in supernatant were all higher. CONCLUSION: CD40 ligand-PLL carrier system may delivery CpG ODN targeting to B lymphocytes, enhancing its immunological efficiency.

AIM: To predict MHC class Ⅰ binding peptides by using neural network ensembles. METHODS: As a combination of neural networks, neural network ensemble (NNE) was here used to improve the predictive performance. Based on a database of 628 nonamers and their classified binding capacities, the generalized NNEs were used to classify peptides respectively with non, low, moderate and high binding capacities to MHC class I molecule encoded by gene HLA-A*0201. The predictive power of NNE was further evaluated by running generalized NNE on a set of actual T-cell epitopes. RESULTS: The generalized NNEs achieved an average predictive hit rate of 0.8 for the above classifications. In addition, NNE was also efficient in the prediction of the potential T-cell epitopes, and about 84% of the actual T-cell epitopes were among the potentially antigenic peptides with high and moderate affinities. CONCLUSION: The NNEs can be applied in the prediction of MHC class Ⅰ binding peptides, and moreover, after proper modifications, they can be conveniently extended to cover peptides with any length and thus suitable for the prediction of peptides binding to other MHC class Ⅰ or even class Ⅱ molecules.

AIM: To address whether the analysis of CD45~+CD86~+ cells isolated from para-aortic lymph nodes (PLNs) is valuable in assessment of the status of local immunity at the feto-maternal interface. METHODS: CBA/J?DBA/2, virgin CBA/J, and CBA/J?BALB/c mice were used as an abortion-prone model (group A), non-pregnant controls (group N), and fertile controls (group F), respectively. The percentage of CD45~+CD86~+ cell in the CD45~+ cell group (CD45~+CD86~+ percentage for short) and the absolute number of these cells were determined with flow cytometry (FCM), using mononuclear cells isolated from PLNs collected on day 5.5, 9.5, and 13.5 of gestation, respectively, and mononuclear cells from placentas on day 13.5 of gestation. To clarify the identity of these CD86~+ cells, FCM was also performed with CD3, CD19 and DX5 as markers for T cells, B cells, and NK cells, respectively. RESULTS: Both resorption rate and absolute number of resorption were significantly higher in group A (29.3%, 1.8?1.0) than those in group F (4.8%, 0.3?0.5, P