Objective:To investigate the effect of bone morphogenetic protein-9(BMP9)overexpression on inflammatory response and apoptosis of alveolar epithelial cells induced by lipopolysaccharide(LPS).Methods:A549 cells were stimulated with 0.1 μg/ml LPS,expressions and changes of BMP9 protein at different time points(LPS stimulation for 0 h,6 h,12 h,24 h)were detected by Western blot.Expression of BMP9 in A549 cells was up-regulated by transfection of BMP9 plasmid,and the transfection efficiency was verified by Western blot and qPCR.After 12 h of LPS stimulation,levels of inflammatory factors TNF-α,IL-6,IL-1β in cell supernatant were detected by ELISA,expressions of anti-apoptotic protein(Bcl-2)and pro-apoptotic protein(Bax)were detected by Western blot,and apoptosis of cells was detected by TUNEL staining.Results:With the extension of LPS stimulation time,expression of BMP9 was down-regulated.Overexpression of BMP9 successfully up-regulated expression of BMP9 in A549 cells.LPS stimulation promoted secretions of TNF-α,IL-6 and IL-1β from A549 cells,increased apoptosis,promoted Bcl-2 expression while inhibited Bax expression.Overexpression of BMP9 inhibited TNF-α,IL-6 and IL-1β releasing,decreased apoptosis,inhibited Bcl-2 expression,while promoted Bax expression.Conclusion:In LPS-stimulated A549 cells,BMP9 expression is gradually decreased at a time-depen-dent manner.Overexpression of BMP9 can inhibit LPS-induced inflammatory response and apoptosis in A549 cells.

Objective:To establish a sterility test method for epinastine hydrochloride eye drops.Methods:Accord-ing to the Pharmacopoeia ofthe People's Republic ofChina 2020,the sterility test method of this product was estab-lished by membrane filtration method,polysorbate 80 was selected as the neutralizing agent,and the washing amount was screened to establish the sterility test method of this variety.Results:Taking 0.1%sterile peptone aqueous solu-tion containing 1%polysorbate 80 as the flushing solution,the flushing volume is 100 mL each time,and the total flushing volume of each membrane is 500 mL,which can eliminate the antibacterial effect.Conclusion:This method can effectively remove the bacteriostasis of epinastine hydrochloride eye drops,and is suitable for the sterility inspec-tion of this variety.

Objective:To establish a sterility test method for epinastine hydrochloride eye drops.Methods:Accord-ing to the Pharmacopoeia ofthe People's Republic ofChina 2020,the sterility test method of this product was estab-lished by membrane filtration method,polysorbate 80 was selected as the neutralizing agent,and the washing amount was screened to establish the sterility test method of this variety.Results:Taking 0.1%sterile peptone aqueous solu-tion containing 1%polysorbate 80 as the flushing solution,the flushing volume is 100 mL each time,and the total flushing volume of each membrane is 500 mL,which can eliminate the antibacterial effect.Conclusion:This method can effectively remove the bacteriostasis of epinastine hydrochloride eye drops,and is suitable for the sterility inspec-tion of this variety.

Objective:To investigate the effect of bone morphogenetic protein-9(BMP9)overexpression on inflammatory response and apoptosis of alveolar epithelial cells induced by lipopolysaccharide(LPS).Methods:A549 cells were stimulated with 0.1 μg/ml LPS,expressions and changes of BMP9 protein at different time points(LPS stimulation for 0 h,6 h,12 h,24 h)were detected by Western blot.Expression of BMP9 in A549 cells was up-regulated by transfection of BMP9 plasmid,and the transfection efficiency was verified by Western blot and qPCR.After 12 h of LPS stimulation,levels of inflammatory factors TNF-α,IL-6,IL-1β in cell supernatant were detected by ELISA,expressions of anti-apoptotic protein(Bcl-2)and pro-apoptotic protein(Bax)were detected by Western blot,and apoptosis of cells was detected by TUNEL staining.Results:With the extension of LPS stimulation time,expression of BMP9 was down-regulated.Overexpression of BMP9 successfully up-regulated expression of BMP9 in A549 cells.LPS stimulation promoted secretions of TNF-α,IL-6 and IL-1β from A549 cells,increased apoptosis,promoted Bcl-2 expression while inhibited Bax expression.Overexpression of BMP9 inhibited TNF-α,IL-6 and IL-1β releasing,decreased apoptosis,inhibited Bcl-2 expression,while promoted Bax expression.Conclusion:In LPS-stimulated A549 cells,BMP9 expression is gradually decreased at a time-depen-dent manner.Overexpression of BMP9 can inhibit LPS-induced inflammatory response and apoptosis in A549 cells.

Objective:To investigate the efficacy of hemodiafiltration combined with hemoperfusion in the treatment of secondary hyperparathyroidism (SHPT) in patients undergoing maintenance hemodialysis (MHD).Methods:A total of 40 patients with SHPT undergoing MHD who received treatment at the Blood Purification Center of The First Affiliated Hospital of Anhui University of Science and Technology from February 2021 to March 2023 were included in this prospective cohort study. They were randomly divided into a control group and an observation group ( n = 20/group).The control group received a single high flux hemodialysis, while the observation group used a combination of hemodialysis filtration and hemoperfusion for 3 months. In both groups, the changes in hemoglobin, blood urea nitrogen, serum creatinine, serum calcium, serum phosphorus,and parathyroid hormone levels were compared before and after dialysis. Results:After dialysis, the hemoglobin level in the observation group was (119.45 ± 5.27) g/L, which was significantly higher than (106.30 ± 6.52) g/L in the control group ( t = -7.02, P < 0.001). The serum phosphorus level in the observation group was (1.18 ± 0.17) mmol/L, which was significantly lower than (1.52 ± 0.22) mmol/L in the control group ( t = 5.49, P < 0.001). The parathyroid hormone level in the observation group was (122.14 ± 40.57) ng/L, which was significantly lower than (168.78 ± 78.27) ng/L in the control group ( t = 2.39, P = 0.023). Conclusion:Hemodiafiltration combined with hemoperfusion can reduce clinical symptoms, increase hemoglobin level, and reduce phosphorus and parathyroid hormone levels in patients with SHPT undergoing MHD, which deserves clinical promotion.

Objective To evaluate the clinical effect of superior trapezius myocutaneous (STM) flap with thetransverse cervical artery (TCA) in reconstruction of tongue defects immediately after the neck dissection of tongue squamous cell carcinoma.Methods From January 2013 to December 2015,the combined radical neck dissection was carried out in 16 patients of tongue squamous cell carcinoma in our institute.All tongue defects were reconstructed by the STM with the TCA.The donor sites were closed directly without skin grafting.Results All flaps survived,and the success rate was 100％.Primarily,the morphology and function of the reconstructed tongues were dissatisfactory.Three months to one year after operation,the mucosalization of flaps appeared,and the functions of speech,mastication and swallowing improved obviously.Conclusion The STM with TCA is one of the ideal flap for reconstructing the postoperative defects of tongue squamous cell carcinoma.

Objective To explore the clinical effects of superior trapezius myocutaneous flap through accessory nerve anastomosis in immediately repairing the tongue defect after tongue squamous cell carcinoma operation.Methods Six cases of tongue squamous cell carcinoma diagnosed by pathology in the Maxillofacial Surgery Department of the Affiliated Stomatological Hospital of Chongqing Medical University from January 2015 to December 2016 were collected and Performed the tongue-neck combined radical operation.The superior trapezius myocutaneous flap simultaneous repair of tongue defect with accessory nerve and hypoglossal anastomosis was adopted during operation.The wound surface of donor site was directly sutured without skin grafting.Results All flaps survived.After 6 months to one year,the flap became mucolized,the surface of flap was similar to the normal oral mucosa with good character.The movement of reconstructed tongue mainly depended on the movement of residual tongue at early stage (within postoperative 3 months).After 6 monthsthe reconstructed tongue had obvious mobility,but the flexibility was poorer than that of the uninjured side,and the patient was satisfied with the language and swallowing function.Conclusion The superior trapezius myocutaneous flap through the accessory nerve anastomosis is one of the ideal skin flaps for repairing tongue defect after e tongue squamous cell carcinoma operation.