Attempts have been made to modulate motor sequence learning (MSL) through repetitive transcranial magnetic stimulation, targeting different sites within the sensorimotor network. However, the target with the optimum modulatory effect on neural plasticity associated with MSL remains unclarified. This study was therefore designed to compare the role of the left primary motor cortex and the left supplementary motor area proper (SMAp) in modulating MSL across different complexity levels and for both hands, as well as the associated neuroplasticity by applying intermittent theta burst stimulation together with the electroencephalogram and concurrent transcranial magnetic stimulation. Our data demonstrated the role of SMAp stimulation in modulating neural communication to support MSL, which is achieved by facilitating regional activation and orchestrating neural coupling across distributed brain regions, particularly in interhemispheric connections. These findings may have important clinical implications, particularly for motor rehabilitation in populations such as post-stroke patients.
Humans ; Transcranial Magnetic Stimulation ; Motor Cortex/physiology* ; Male ; Electroencephalography ; Neuronal Plasticity/physiology* ; Female ; Adult ; Evoked Potentials, Motor/physiology* ; Young Adult ; Learning/physiology*

Objective:To investigate the effect of GBP3 on replication of adult T-lymphocytic leukemia virus type 1(HTLV-1).Methods:Expression of GBP3 in HTLV-1-infected HeLa cell and THP1 cell was detected by Western blot.The knock-down efficiency of siRNAs targeting GBP3 in HeLa and THP1 cells was evaluated by Western blot.Effects of GBP3 overexpression or knockdown on expression of HTLV-1 proviral transcripts Tax,px,HBZ,Gag,ENV,5'UTR,and viral proteins Tax,p19 were investigated by RT-qPCR and Western blot.GTPase-defective mutant of GBP3,GBP3K51A was constructed to explore whether the effects of GBP3 on HTLV-1 infection were dependent on its GTPase activity.Results:GBP3 expression was upregulated in HTLV-1 infected HeLa and THP1 cells.GBP3 overexpression decreased expression of HTLV-1 proviral transcripts and viral proteins,whereas the knockdown of GBP3 has the opposite effects.Overexpression of GBP3K51A increased expression of HTLV-1 proviral transcripts and viral proteins.Conclusion:HTLV-1 virus infection can induce expression of GBP3;overexpression of GBP3 inhibits virus replication and may depend on GTPase.

Objective:To investigate the effect of GBP3 on replication of adult T-lymphocytic leukemia virus type 1(HTLV-1).Methods:Expression of GBP3 in HTLV-1-infected HeLa cell and THP1 cell was detected by Western blot.The knock-down efficiency of siRNAs targeting GBP3 in HeLa and THP1 cells was evaluated by Western blot.Effects of GBP3 overexpression or knockdown on expression of HTLV-1 proviral transcripts Tax,px,HBZ,Gag,ENV,5'UTR,and viral proteins Tax,p19 were investigated by RT-qPCR and Western blot.GTPase-defective mutant of GBP3,GBP3K51A was constructed to explore whether the effects of GBP3 on HTLV-1 infection were dependent on its GTPase activity.Results:GBP3 expression was upregulated in HTLV-1 infected HeLa and THP1 cells.GBP3 overexpression decreased expression of HTLV-1 proviral transcripts and viral proteins,whereas the knockdown of GBP3 has the opposite effects.Overexpression of GBP3K51A increased expression of HTLV-1 proviral transcripts and viral proteins.Conclusion:HTLV-1 virus infection can induce expression of GBP3;overexpression of GBP3 inhibits virus replication and may depend on GTPase.

Objective To explore the application effect of prospective nursing in patients with hip replacement operation.Methods 80 patients with hip replacement operation were ran-domly divided into observation group and control group with 40 patients in each group.The control group was received routine nursing while the observation group was given prospective nursing based on the treatment in the control group.Rehabilitation enthusiasm,complications,hospitalization time,life quality and nursing satisfaction were compared between the two groups.Results Reha-bilitation enthusiasm score in the observation group was higher than that in the control group,com-plications and hospitalization time were less than those in the control group,nursing satisfaction was higher than that in the control group ,thus ,the difference was statistical significant (P <0.05).Life quality score in physical function,social function,mental function in the observation group were higher than that in the control group ,the difference was statistical significant (P <0.05).Conclusion Prospective nursing is preventive and targeted nursing.And it can effectively improve the rehabilitation enthusiasm of hip replacement operation patients and enhance the life quality.

Objective To explore the application effect of prospective nursing in patients with hip replacement operation.Methods 80 patients with hip replacement operation were ran-domly divided into observation group and control group with 40 patients in each group.The control group was received routine nursing while the observation group was given prospective nursing based on the treatment in the control group.Rehabilitation enthusiasm,complications,hospitalization time,life quality and nursing satisfaction were compared between the two groups.Results Reha-bilitation enthusiasm score in the observation group was higher than that in the control group,com-plications and hospitalization time were less than those in the control group,nursing satisfaction was higher than that in the control group ,thus ,the difference was statistical significant (P <0.05).Life quality score in physical function,social function,mental function in the observation group were higher than that in the control group ,the difference was statistical significant (P <0.05).Conclusion Prospective nursing is preventive and targeted nursing.And it can effectively improve the rehabilitation enthusiasm of hip replacement operation patients and enhance the life quality.