Objective To investigate the relationship between serum levels of silent information regulator 1(SIRT1)and(sex determining region Y-box protein 2(SOX2)and liver metastasis one year after colorectal cancer surgery.Methods A total of 112 patients who underwent laparoscopic radical operation for colorectal cancer in Southern Theater General Hospital from December 2020 to February 2023 were selected as research objects.Serum SIRT1 and SOX2 levels were detected one day before surgery.Liver metastasis was recorded during one-year follow-up.The patients with liver metastasis were included in the observation group,while patients without liver metastasis were assigned to the control group.The relationship between serum levels of SIRT1 and SOX2 and liver metastasis after colon cancer surgery and the evalution value of both for liver metastasis after surgery in colon cancer patients were analyzed.Results Six patients failed to be followed up,33 patients(31.13%)suffered from liver metastasis one year after surgery(observation group),and the other 73 patients without liver metastasis were included in control group.The observation group had lower SIRT1 and higher SOX2 than the control group(P<0.05).After adjustment of differentiated degree,depth of invasion,and lymph node metastasis,the risk of postoperative liver metastasis in colon cancer patients increased by 1.123 and 1.925 times,respectively(P<0.05),when SIRT1 decreased by 1%and SOX2 increased by 1%,with logit(P)=-1.938-0.754×SIRT1+1.073×SOX2.The area under curve of SIRT1 combined with SOX2 for the evaluation of postoperative liver metastasis in colon cancer patients was greater than SIRT1 and SOX2 alone(Z=2.107,P=0.035;Z=2.481,P=0.013).Conclusion Decreased SIRT1 and increased SOX2 may increase the risk of postoperative liver metastasis in patients with colon cancer.SIRT1 and SOX2 have the potential to serve as predictive indicators for liver metastasis after surgery in colon cancer patients.

Objective To investigate the expression levels of serum G protein-coupled receptor-associated sorting protein 1(GASP-1),lipocalin-2(LCN2)and targeting protein for Xenopus kinesin-like protein 2(TPX2)in lung cancer patients,and their correlations with tumor-node-metastasis classification(TNM)pathological stage and prognosis.Methods A total of 92 patients with lung cancer who were admitted to General Hospital of Southern Theatre Command from April 2019 to May 2022 were selected as observation group.Meanwhile,92 healthy subjects were selected as control group.Peripheral venous blood samples of the two groups were collected to detect serum GASP-1,LCN2 and TPX2 levels.Serum GASP-1,LCN2 and TPX2 levels of lung cancer patients with different TNM stages were analyzed.According to the prognosis,the patients were divided into good prognosis group and poor prognosis group.Serum GASP-1,LCN2 and TPX2 levels were compared between the two groups.The correlations of serum GASP-1,LCN2 and TPX2 levels with TNM stage and prognosis in patients with lung cancer were investigated.Results The serum GASP-1,LCN2 and TPX2 levels in the observation group were significantly higher than those in the control group(P＜0.05).The serum levels of GASP-1,LCN2 and TPX2 in patients with TNM stage Ⅲ and Ⅳ were significantly higher than those in patients with TNM stage Ⅰand Ⅱ(P＜0.05).The levels of serum GASP-1,LCN2 and TPX2 in patients with TNM stage Ⅳ were significantly higher than those in patients with TNM stage Ⅲ(P＜0.05).Serum GASP-1,LCN2 and TPX2 levels in the good prognosis group were significantly lower than those in the poor prognosis group(P＜0.05).Serum GASP-1,LCN2 and TPX2 levels were positively correlated with TNM stage before treatment(P＜0.05),but were negatively correlated with the prognosis after treatment(P＜0.05).Conclusion Serum levels of GASP-1,LCN2 and TPX2 may be related to the TNM pathological stages of lung cancer patients.The detection of the above serum indicators after treatment can reflect the prognosis of patients to a certain extent,and provide reference for clinical practice.

Objective To explore the value of detecting serum cytokeratin 19 fragmen 21-1 (CYFRA21-1) and squamous cell carcinoma antigen (SCCAg) in the diagnosis of patients with nasopharyngeal carcinoma.Methods 79 patients with nasopharyngeal carcinoma from October 2013 to October 2015 as case group and 145 patients with nasopharyngeal chronic inflammation as control group were collected.The CYFRA21-1 level was detected by the electrochemical luminescence immunoassay and the SCCAg level was detected by the enzymoimmunoassay.Comparisons of diagnostic value of CYFRA21-1 and SCCAg to nasopharyngeal carcinoma were conducted according to pathological diagnosis.Results The serum CYFRA21-1 and SCCAg levels in case group were (12.37±1.65) g/L and (1.65±0.37) ng/ml,and the control group were (4.26±1.99) g/L and (1.22±0.45) ng/ml,with statistically significant differences (t =8.093,P =0.002;t =4.287,P =0.043).The sensitivity,specificity,positive predictive value,negative predictive value and accuracy of CYFRA21-1 combined with SCCAg in the diagnosis of nasopharyngeal carcinoma were higher than those of CYFRA21-1 and SCCAg respectively,and the difference all were statistically significant (all P ＜ 0.05).Conclusion Detecting serum CYFRA21-1 combined with SCCAg in the diagnosis of nasopharyngeal carcinoma has high sensitivity and good specificity,which can significantly improve the diagnostic accuracy.

Objective Amniotic membrane as the carrier to culture the vascular endothelial cells was investigated in this study in order to explore whether the corneal endothelial cells superseded by the vascular endothelial cells is feasible on the account of directing a kind of the original method to handle the bullous keratopathy.Methods The vascular endothelial cells adhering to the sphagitides lumen of the experimental rabbits were digested and gained by means of the perfusion method with 0.25%tripsin plus 0.02%EDTA.Fresh amniotic membrane without the amniotic epithelial cells was cut into the square tissue about 1.5 cm?1.5 cm and spread evenly in the 24-well culture dish.Primary cultured cells were subcultured on the amniotic membrane.We would not transplant the vascular endothelial cells feeding on the amniotic membrane until cells is full of the whole amniotic membrane surface.One to two months after the operation,the change of corneal diaphaneity was observed.Results About 12 days since the cell transplantation was performed,the corneal transparency alteration between the experimental groups and the control one is different.The corneal buttons in the experimental group show the severe edema and opacity,and the anterior chamber couldn't be seen unclearly.But,10 days after the operation,the corneal oedema which begins to extenuate was judged through the indicatrix that the corneal edema in the experimental group has been recovering slowly,among which the anterior chamber tissue of 7 animals was visible through the implant.The corneal edema in the control groups intensified evidently,even,the part of these appeared the ulcerous necrosis as result of the corneal severely edema.There is the existence of difference between two groups(P