Objective This study aimed to compare the osteogenic performance differences of titanium surface coat-ings modified by dopamine or silanized graphene oxide,and to provide a more suitable modification scheme for ti-tanium surface graphene oxide coatings.Methods Tita-nium was subjected to alkali-heat treatment and then modified with dopamine and silanization,respectively,followed by coating with graphene oxide.Control and ex-perimental groups were designed as follows:pure titanium(Ti)group;titanium after alkali-heat treatment(Ti-NaOH)group;titanium after alkali-heat treatment and silanization modification(Ti-APTES)group;titanium after alkali-heat treatment and dopamine modification(Ti-DOPA)group;titanium with silanization-modified surface decorated with gra-phene oxide(Ti-APTES/GO)group;titanium with dopamine-modified surface decorated with graphene oxide(Ti-DOPA/GO)group.The physical and chemical properties of the material surfaces were analyzed using scanning electron micros-copy(SEM),contact angle goniometer,X-ray photoelectron spectroscopy(XPS),and Raman spectrometer.The prolifera-tion and adhesion morphology of mouse embryonic osteoblast precursor cells MC3T3-E1 on the material surfaces were observed by cell viability detection and immunofluorescence staining followed by laser confocal microscopy.The effects on the osteogenic differentiation of MC3T3-E1 cells were studied by alkaline phosphatase(ALP)staining,alizarin red staining and quantification,and real-time quantitative polymerase chain reaction.Results After modification with gra-phene oxide coating,a thin-film-like structure was observed on the surface under SEM.The hydrophilicity of all experi-mental groups was improved,among which the Ti-DOPA/GO group had the best hydrophilicity.XPS and Raman spec-troscopy analysis showed that the modified materials exhibited typical D and G peaks,and XPS revealed the presence of a large number of oxygen-containing functional groups on the surface.CCK8 assay showed that all groups of materials had no cytotoxicity,and the proliferation level of the Ti-APTES/GO group was higher than that of the Ti-DOPA/GO group.Under the laser confocal microscope,the cells in the Ti-DOPA/GO and Ti-APTES/GO groups spread more fully.The Ti-DOPA/GO and Ti-APTES/GO groups had the deepest ALP staining,and the Ti-APTES/GO group had the most alizarin red-stained mineralized nodules and the highest quantitative result of alizarin red staining.In the Ti-DOPA/GO and Ti-APTES/GO groups,the expression of the early osteogenic-related gene RUNX2 reached a relatively high level,while in the expression of the late osteogenic-related genes OPN and OCN,the Ti-APTES/GO group performed better than the Ti-DOPA/GO group.Conclusion Ti-APTES/GO significantly outperformed Ti-DOPA/GO in promoting the adhesion,proliferation,and in vitro osteogenic differentiation of MC3T3-E1 cells.

BackgroundWith the rapid development of the networking technologies， internet addiction has increasingly become a serious mental health issue. Previous studies have revealed the link between childhood trauma and internet addiction， while the mediating role of perceived stress in this link is not yet clear. ObjectiveTo investigate the role of medical students' perceived stress in the relationship between childhood trauma and internet addiction， so as to provide references for the intervention of internet addiction. MethodsFrom February to March 2023， a random sampling technique was used to select 1 232 undergraduate students from the School of Clinical Medical Sciences of Southwest Medical University as research subjects. The Childhood Trauma Questionnaire-Short Form （CTQ-SF）， Perceived Stress Scale （PSS）， Internet Gaming Disorder Scale （IGDS）， and Bergen Social Media Addiction Scale （BSMAS） were used for assessment. Pearson's correlation coefficients were calculated. The mediation effect of perceived stress in the relationship between childhood trauma and internet addiction was tested using Model 4 in the SPSS Process 4.1， and Bootstrapping procedure involving 5 000 replicates was employed to confirm the statistical significance. ResultsA total of 1 016 （82.47%） valid completed questionnaires were gathered. The CTQ-SF scores of medical students were positively correlated with PSS scores， IGD scores， and BSMAS scores （r=0.583， 0.474， 0.465， P<0.01）. PSS scores were positively correlated with IGD scores and BSMAS scores （r=0.369， 0.479， P<0.01）. Childhood trauma in medical students was found to positively predict perceived stress （β=0.191， P<0.01）， social media addiction （β=0.160， P<0.01）， and internet gaming disorder （β=0.106， P<0.01）. Perceived stress played a significant mediating role in the relationship between childhood trauma and internet gaming disorder， indirect effect value was 0.018 （95% CI： 0.009~0.027）， accounting for 16.98%. Perceived stress also exhibited a significant mediating role in the relationship between childhood trauma and social media addiction， indirect effect value was 0.063 （95% CI： 0.048~0.079）， accounting for 39.38%. ConclusionChildhood trauma in medical students may affect internet gaming disorder and social media addiction through perceived stress. ［Funded by 2022 Annual Research Project of Sichuan Applied Psychology Research Center，（number，CSXL-22102）］

OBJECTIVES: This study aimed to compare the osteogenic performance differences of titanium surface coatings modified by dopamine or silanized graphene oxide, and to provide a more suitable modification scheme for titanium surface graphene oxide coatings. METHODS: Titanium was subjected to alkali-heat treatment and then modified with dopamine and silanization, respectively, followed by coating with graphene oxide. Control and experimental groups were designed as follows: pure titanium (Ti) group; titanium after alkali-heat treatment (Ti-NaOH) group; titanium after alkali-heat treatment and silanization modification (Ti-APTES) group; titanium after alkali-heat treatment and dopamine modification (Ti-DOPA) group; titanium with silanization-modified surface decorated with graphene oxide (Ti-APTES/GO) group; titanium with dopamine-modified surface decorated with graphene oxide (Ti-DOPA/GO) group. The physical and chemical properties of the material surfaces were analyzed using scanning electron microscopy (SEM), contact angle goniometer, X-ray photoelectron spectroscopy (XPS), and Raman spectrometer. The proliferation and adhesion morphology of mouse embryonic osteoblast precursor cells MC3T3-E1 on the material surfaces were observed by cell viability detection and immunofluorescence staining followed by laser confocal microscopy. The effects on the osteogenic differentiation of MC3T3-E1 cells were studied by alkaline phosphatase (ALP) staining, alizarin red staining and quantification, and real-time quantitative polymerase chain reaction. RESULTS: After modification with graphene oxide coating, a thin-film-like structure was observed on the surface under SEM. The hydrophilicity of all experimental groups was improved, among which the Ti-DOPA/GO group had the best hydrophilicity. XPS and Raman spectroscopy analysis showed that the modified materials exhibited typical D and G peaks, and XPS revealed the presence of a large number of oxygen-containing functional groups on the surface. CCK8 assay showed that all groups of materials had no cytotoxicity, and the proliferation level of the Ti-APTES/GO group was higher than that of the Ti-DOPA/GO group. Under the laser confocal microscope, the cells in the Ti-DOPA/GO and Ti-APTES/GO groups spread more fully. The Ti-DOPA/GO and Ti-APTES/GO groups had the deepest ALP staining, and the Ti-APTES/GO group had the most alizarin red-stained mineralized nodules and the highest quantitative result of alizarin red staining. In the Ti-DOPA/GO and Ti-APTES/GO groups, the expression of the early osteogenic-related gene RUNX2 reached a relatively high level, while in the expression of the late osteogenic-related genes OPN and OCN, the Ti-APTES/GO group performed better than the Ti-DOPA/GO group. CONCLUSIONS Ti-APTES/GO significantly outperformed Ti-DOPA/GO in promoting the adhesion, proliferation, and in vitro osteogenic differentiation of MC3T3-E1 cells.
Titanium/chemistry* ; Graphite/chemistry* ; Dopamine/chemistry* ; Animals ; Mice ; Osteogenesis ; Osteoblasts/cytology* ; Surface Properties ; Cell Proliferation ; Silanes/chemistry* ; Cell Adhesion ; Coated Materials, Biocompatible/chemistry* ; Cell Differentiation ; Alkaline Phosphatase/metabolism* ; Microscopy, Electron, Scanning

Objective To investigate the value of IL-27,IL-29 and miRNA-497 in HER-2 positive radioactive particles combined targeted therapy. Methods Sixty-one elderly inpatients with HER-2 positive gastric cancer ascites in the Gaoming District People's Hospital, Wuchuan Municipal People's Hospital and Zhejiang Provincial Tumor Hospital were divided into the two groups. The treatment group(n= 31) was treated by the radioactive particles+ targeted drug Trastuzumab(initial dose 8 mg/kg, maintenance dose 66 mg/kg, once per 3 weeks) for 6 weeks; the control group(30 cases) was treated by targeted drug Trastuzumab (initial dose 8 mg/kg,maintenance dose 6 mg/kg, once per 3 weeks) for 6 weeks. The levels of ascites IL-27,IL-29 and miRNA-497 in the radioactive partcles combined targeted therapy group and control group were detected. Results The levels of ascites IL-27, IL-29 and miRNA-497 after treatment in the treatment group were significantly decreased compared with those before treatment and in the control group,the effective remission rate in the treatment group was 74. 19%, which was higher than 36.67% in the control group, the average median survival time in the treatment group was 155 d, which washigher than 72 d in the control group, the difference was statistically significant. Conclusion IL-27 ,IL-29 and miRNA-497 has the significance of curative effect evaluation and prognosis judgment in the radioactive particles combined targeted therapy for the patients with HER 2 positive gastric cance.

Objective To observe the effects of compound rhodiola sachalinensis A Bor (cRSAB) pretreatment on the serum levels of Interleukin -6 ( IL-6) and matrix metalloproteinase-9 ( MMP-9) at high altitude regions, so as to explore the mechanism of cRSAB for the elevation of hypoxia tolerance and the prevention of acute high altitude sickness .Methods Interventions were made on the 8 healthy subjects in accordance with different pressures (sea level pressure or simulated high altitude of 4000 m), exercise (rest state or stepping-stool exercise) and oral taking of cRSAB (7 days or 14 days).The subjects were divided into 7 groups: the conventional pressure at rest group (or group A), the conventional pressure exercise group (or group B), the hypobaric at rest group (or group C); the hypobaric exercise group (or group D), the oral-taking of cRSAB for 14 days at rest group (or group E), the oral-taking of cRSAB for 7 days exercise group (or group F), the oral-taking of cRSAB for 14 days exercise group (or group G).Then, serum concentrations of IL-6 and MMP-9 at different conditions were detected by ELISA .Results As compared with those of group A[IL-6(27.93 ±1.24)pg/ml, MMP-9(270.08 ±10.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the subjects of group C [(28.96 ±1.38) pg/ml], MMP-9 [(281.50 ±12.31) ng/ml] were elevated significantly.When compared with those of group B [ IL-6 (27.74 ±1.06) pg/ml, MMP-9 (269.88 ± 9.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the group D subjects [(29.56 ±1.26)pg/ml], MMP-9 [(285.25 ±11.67) ng/ml] increased obviously.Statistical significance could be noticed , when comparisons were made between them ( P <0.05).The serum concentrations of IL -6 and MMP-9 in the subjects of group C increased, as compared with those in the subjects of group D , with statistical significance (P <0.05).At the simulated high altitude of 4000 m, the serum concentrations of IL-6 and MMP-9 in the subjects of group F and group G all decreased considerably , when compared with those in subjects of group D , also with statistical significance (P <0.05).There was no statistical significance in serum concentrations of IL -6 and MMP-9, when comparisons were made between the subjects of group F and group G ( P >0.05). Conclusions At hypobaric pressure, the serum concentrations of IL-6 and MMP-9 in the subjects with exercise increased obviously.IL-6 and MMP-9 might be associated with the mechanism of cRSAB in the protective effect of low pressures on human motor function .

Objective To observe the effects of compound rhodiola sachalinensis A Bor (cRSAB) pretreatment on the serum levels of Interleukin -6 ( IL-6) and matrix metalloproteinase-9 ( MMP-9) at high altitude regions, so as to explore the mechanism of cRSAB for the elevation of hypoxia tolerance and the prevention of acute high altitude sickness .Methods Interventions were made on the 8 healthy subjects in accordance with different pressures (sea level pressure or simulated high altitude of 4000 m), exercise (rest state or stepping-stool exercise) and oral taking of cRSAB (7 days or 14 days).The subjects were divided into 7 groups: the conventional pressure at rest group (or group A), the conventional pressure exercise group (or group B), the hypobaric at rest group (or group C); the hypobaric exercise group (or group D), the oral-taking of cRSAB for 14 days at rest group (or group E), the oral-taking of cRSAB for 7 days exercise group (or group F), the oral-taking of cRSAB for 14 days exercise group (or group G).Then, serum concentrations of IL-6 and MMP-9 at different conditions were detected by ELISA .Results As compared with those of group A[IL-6(27.93 ±1.24)pg/ml, MMP-9(270.08 ±10.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the subjects of group C [(28.96 ±1.38) pg/ml], MMP-9 [(281.50 ±12.31) ng/ml] were elevated significantly.When compared with those of group B [ IL-6 (27.74 ±1.06) pg/ml, MMP-9 (269.88 ± 9.90)ng/ml], the serum concentrations of IL -6 and MMP-9 in the group D subjects [(29.56 ±1.26)pg/ml], MMP-9 [(285.25 ±11.67) ng/ml] increased obviously.Statistical significance could be noticed , when comparisons were made between them ( P <0.05).The serum concentrations of IL -6 and MMP-9 in the subjects of group C increased, as compared with those in the subjects of group D , with statistical significance (P <0.05).At the simulated high altitude of 4000 m, the serum concentrations of IL-6 and MMP-9 in the subjects of group F and group G all decreased considerably , when compared with those in subjects of group D , also with statistical significance (P <0.05).There was no statistical significance in serum concentrations of IL -6 and MMP-9, when comparisons were made between the subjects of group F and group G ( P >0.05). Conclusions At hypobaric pressure, the serum concentrations of IL-6 and MMP-9 in the subjects with exercise increased obviously.IL-6 and MMP-9 might be associated with the mechanism of cRSAB in the protective effect of low pressures on human motor function .

Objective To explore the measures and methods of the health management of the deep saturation diver candidates,and also to evaluate the effects of health management on the divers.Methods A professional health management was enforced on 37 saturation diver candidates who were to perform the 300 m saturation dive from June to December 2014.A comprehensive health evaluation on the changes in physical examination indexes,psychological symptoms scores,physical performance reserve and health assessment results was respectively made in June 2014 (before the implementation of health management) and December 2014 (6 months after the implementation of health management) by using the diver life style health promotion questionnaire survey.Results As compared with that before the implementation of professional health management,the diver health promotion life style was obviously improved following the implementation of professional health management.There was statistical significance in self-realization,interpersonal relationship,health obligations and stress management,when comparisons were made between pre and post implementation of health management.Physical performance reserve was considerably improved,and statistical significance could be noted in such indicators as push-ups,sit-ups and ground rotation.The qualified health rate was increased from 64.86％ to 81.08％,and the psychological factor was well maintained,which reached operational standards of saturation diving.Conclusions Systematic and scientific occupational health management of saturation divers could effectively promote their physical and mental health and was beneficial to the maintenance of their operational capability.

Objective To explore the measures and methods of the health management of the deep saturation diver candidates,and also to evaluate the effects of health management on the divers.Methods A professional health management was enforced on 37 saturation diver candidates who were to perform the 300 m saturation dive from June to December 2014.A comprehensive health evaluation on the changes in physical examination indexes,psychological symptoms scores,physical performance reserve and health assessment results was respectively made in June 2014 (before the implementation of health management) and December 2014 (6 months after the implementation of health management) by using the diver life style health promotion questionnaire survey.Results As compared with that before the implementation of professional health management,the diver health promotion life style was obviously improved following the implementation of professional health management.There was statistical significance in self-realization,interpersonal relationship,health obligations and stress management,when comparisons were made between pre and post implementation of health management.Physical performance reserve was considerably improved,and statistical significance could be noted in such indicators as push-ups,sit-ups and ground rotation.The qualified health rate was increased from 64.86％ to 81.08％,and the psychological factor was well maintained,which reached operational standards of saturation diving.Conclusions Systematic and scientific occupational health management of saturation divers could effectively promote their physical and mental health and was beneficial to the maintenance of their operational capability.

OBJECTIVETo explore the effects of hyperbaric oxygen preconditioning (HBOP) on human stress responses during acute exposure to high altitude and the possible mechanism.

METHODSEight male subjects were treated with HBOP for 3, 5, and 7 days, followed by acute exposure to hypoxia simulating an altitude of 4,000 m. Subjects at rest were divided into sea-level control group, simulated high-altitude group, and 5-day HBOP intervention group, while subjects after physical load were divided into sea-level control group, simulated high-altitude group, 3-day HBOP intervention group, and 7-day HBOP intervention group. The physical load test was performed for each subject before and after HBOP, and the plasma levels of dopamine (DA), epinephrine (E), norepinephrine (NE), and adrenocorticotropic hormone (ACTH) were determined before and after exercise. The physical load test was performed by stepping up on to a 30 cm-high stepping stool at a rate of 25/min for 5 minutes, which was a type of moderate physical exercise. The stepping rate and timing were controlled by a metronome.

RESULTSThe levels of DA, E, NE, and ACTH at rest and after physical load were significantly higher in subjects acutely exposed to high altitude than in the sea-level control groups (all P<0.05). Moreover, the levels of DA, E, NE, and ACTH at rest were significantly higher after acute exposure to high altitude in the 5-day HBOP intervention group than in the simulated high-altitude group (all P<0.01). Except for the ACTH level in the 3-day HBOP intervention group, the levels of DA, E, NE, and ACTH after physical load were significantly higher after acute exposure to high altitude in the 3-day and 7-day HBOP intervention groups than in the simulated high-altitude group (all P<0.01).

CONCLUSIONHBOP can elevate the plasma expression of DA, E, NE, and ACTH, and then speed up the establishment of a new balance of homeostasis to adapt to the acute hypoxia at high altitude.

Adrenocorticotropic Hormone ; blood ; Altitude ; Dopamine ; blood ; Epinephrine ; blood ; Exercise ; Homeostasis ; Humans ; Hyperbaric Oxygenation ; Hypoxia ; blood ; Male ; Norepinephrine ; blood ; Rest ; Stress, Physiological

Objective To develop a portable device to monitor and record the exercise quantity of navy serviceman. The device was composed of control components, a oscillator clock, buttons, 3D acceleration sensor and a organic light-emitting diode (OLED) screen. The monitoring involved in two modes of exclusive use and use combined with exercise apparatus.Methods The device could be connected with PC for data transmission through USB interface. Results Trials proved that the device met the desired requirements and could be used for the data acquisition and monitoring of the exercise quantity of the serviceman.ConclusionThe device can record and evaluate the physical fitness training of navy serviceman.