The aim of this experiment is to investigate the mechanism of liver cell damage induced by nonylphenol(NP).After establishing an in vitro model of NP induced BRL-3A liver cell injury,changes of oxidative damage markers were evaluated,Fe2+content was determined,the ultrastruc-ture of BRL-3A was observed,and the expression level of iron death marker proteins were deter-mined.The results showed that NP could significantly increase ROS and MDA in BRL-3A cells(P＜0.05);the GSH content,GSH Px activity,and SOD activity were significantly reduced(P＜0.05);the Fe2+content significantly increased(P＜0.05)and showed a dose-dependent effect;the mitochondrial volume of BRL-3A cells significantly decreases,the mitochondrial cristae break or e-ven disappear,and some mitochondria show vacuolization;the expression levels of iron homeostasis related proteins TFR1 and FTH1 were significantly reduced(P＜0.05).The results indicate that ferroptosis is involved in the mechanism of NP induced liver cell damage,which bene-fits for further exploration of NP pathogenesis.

Objective:To discuss the characteristics of common diseases of fighter pilots and provide evidence and support for improving the health level and combat capabilities of Chinese fighter pilots by the Meta-analysis of disease spectrum.Methods:The information of disease spectrum of Chinese fighter pilots was extracted from the literatures collected by SinoMed, China Academic Journal Network Publishing Database, China Scientific Journal Database, Wanfang Data and PubMed before December of 2020. STATA 16 and SPSS 26 software were used to make system evaluation for the constituent ratio of single disease, and the diseases were analyzed in subgroups by years.Results:Seventeen literatures, that published from 2002 to 2020, were collected for Meta-analysis and 9 431 persons were involved (aged from 20 to 60 years) based on the statistical analysis from 1965 to 2019. The results indicated metabolic diseases, spinal diseases, aviation occupational diseases, hepatobiliary diseases, etc.. The Meta-analysis showed that the proportion of spinal diseases was 29.5% (95 %CI was 20.8%~38.2%, I2=99.0%), metabolic diseases was 22.2% (95 %CI was 12.5%~31.8%, I2=99.5%), valvulopathy and arrhythmia was 15.1% (95 %CI was 7.3%~22.8%, I2=97.0%), aviation occupational diseases was 14.3% (95 %CI was 8.2%~20.4%, I2=94.4%), hepatobiliary diseases was 10.5% (95 %CI was 5.4%~15.5%, I2=98.9%) and gastrointestinal diseases was 8.4% (95 %CI was 5.5%~11.3%, I2=94.0%) in Chinese fighter pilots. Conclusions:The metabolic diseases, spinal diseases and hepatobiliary diseases are accounted for a relatively higher proportion among Chinese fighter pilots, and corresponding protective measures should be taken to improve the health level of pilots through the work of medical support departments at all levels.

Objective:To discuss the characteristics of common diseases of fighter pilots and provide evidence and support for improving the health level and combat capabilities of Chinese fighter pilots by the Meta-analysis of disease spectrum.Methods:The information of disease spectrum of Chinese fighter pilots was extracted from the literatures collected by SinoMed, China Academic Journal Network Publishing Database, China Scientific Journal Database, Wanfang Data and PubMed before December of 2020. STATA 16 and SPSS 26 software were used to make system evaluation for the constituent ratio of single disease, and the diseases were analyzed in subgroups by years.Results:Seventeen literatures, that published from 2002 to 2020, were collected for Meta-analysis and 9 431 persons were involved (aged from 20 to 60 years) based on the statistical analysis from 1965 to 2019. The results indicated metabolic diseases, spinal diseases, aviation occupational diseases, hepatobiliary diseases, etc.. The Meta-analysis showed that the proportion of spinal diseases was 29.5% (95 %CI was 20.8%~38.2%, I2=99.0%), metabolic diseases was 22.2% (95 %CI was 12.5%~31.8%, I2=99.5%), valvulopathy and arrhythmia was 15.1% (95 %CI was 7.3%~22.8%, I2=97.0%), aviation occupational diseases was 14.3% (95 %CI was 8.2%~20.4%, I2=94.4%), hepatobiliary diseases was 10.5% (95 %CI was 5.4%~15.5%, I2=98.9%) and gastrointestinal diseases was 8.4% (95 %CI was 5.5%~11.3%, I2=94.0%) in Chinese fighter pilots. Conclusions:The metabolic diseases, spinal diseases and hepatobiliary diseases are accounted for a relatively higher proportion among Chinese fighter pilots, and corresponding protective measures should be taken to improve the health level of pilots through the work of medical support departments at all levels.