Objective:To investigate the expression level of miR-24-3p in peripheral blood of patients with type 2 diabetic nephropathy (DN) and its clinical significance.Methods:A cross-sectional study was conducted among 100 patients with simple type 2 diabetes mellitus (T2DM group) and 103 patients with DN (DN group). The relative expression level of miR-24-3p in the peripheral blood of patients in both groups was measured. Differences in miR-24-3p expression level among patients with DN were analyzed. The efficacy of miR-24-3p expression level in predicting the occurrence of DN was assessed.Results:The relative expression level of miR-24-3p in the peripheral blood of patients with DN was significantly higher than that of patients with T2DM [(1.43 ± 0.43) vs. (1.07 ± 0.33), t = -6.82, P < 0.05]. Significant differences in miR-24-3p level were observed among DN patients with varying levels of fasting blood glucose, glycated hemoglobin A1c, estimated glomerular filtration rate, and serum creatinine and magnesium levels ( t = -2.92, -2.90, -3.84, -3.41, -4.17, all P < 0.05). There was a statistically significant difference in miR-24-3p level among DN patients with different degrees of renal interstitial inflammation and renal interstitial fibrosis and tubular atrophy scores ( F = 48.73, 27.07, both P < 0.05). Correlation analysis revealed that the relative expression level of miR-24-3p in the peripheral blood of patients with DN was positively correlated with fasting blood glucose, glycated hemoglobin A1c, serum creatinine, renal interstitial inflammation score, and renal interstitial fibrosis and tubular atrophy score ( r = 0.48, 0.57, 0.63, 0.71, 0.69, all P < 0.05). It was negatively correlated with estimated glomerular filtration rate and blood magnesium level ( r = -0.72, -0.78, both P < 0.05). The relative expression level of miR-24-3p in the peripheral blood can be used to predict the occurrence of DN [area under the curve = 0.864, 95% CI: (0.813, 0.916)], and the sensitivity and specificity of this prediction were 85.0% and 79.6%, respectively. Conclusions:The expression of miR-24-3p is high in the peripheral blood of patients with DN, and its level is associated with blood glucose metabolism, blood magnesium level, renal inflammatory injury, renal interstitial fibrosis, and glomerular atrophy. Upregulation of miR-24-3p may increase the risk of renal disease and exacerbate kidney damage in patients with T2DM.

Objective:To investigate the expression level of miR-24-3p in peripheral blood of patients with type 2 diabetic nephropathy (DN) and its clinical significance.Methods:A cross-sectional study was conducted among 100 patients with simple type 2 diabetes mellitus (T2DM group) and 103 patients with DN (DN group). The relative expression level of miR-24-3p in the peripheral blood of patients in both groups was measured. Differences in miR-24-3p expression level among patients with DN were analyzed. The efficacy of miR-24-3p expression level in predicting the occurrence of DN was assessed.Results:The relative expression level of miR-24-3p in the peripheral blood of patients with DN was significantly higher than that of patients with T2DM [(1.43 ± 0.43) vs. (1.07 ± 0.33), t = -6.82, P < 0.05]. Significant differences in miR-24-3p level were observed among DN patients with varying levels of fasting blood glucose, glycated hemoglobin A1c, estimated glomerular filtration rate, and serum creatinine and magnesium levels ( t = -2.92, -2.90, -3.84, -3.41, -4.17, all P < 0.05). There was a statistically significant difference in miR-24-3p level among DN patients with different degrees of renal interstitial inflammation and renal interstitial fibrosis and tubular atrophy scores ( F = 48.73, 27.07, both P < 0.05). Correlation analysis revealed that the relative expression level of miR-24-3p in the peripheral blood of patients with DN was positively correlated with fasting blood glucose, glycated hemoglobin A1c, serum creatinine, renal interstitial inflammation score, and renal interstitial fibrosis and tubular atrophy score ( r = 0.48, 0.57, 0.63, 0.71, 0.69, all P < 0.05). It was negatively correlated with estimated glomerular filtration rate and blood magnesium level ( r = -0.72, -0.78, both P < 0.05). The relative expression level of miR-24-3p in the peripheral blood can be used to predict the occurrence of DN [area under the curve = 0.864, 95% CI: (0.813, 0.916)], and the sensitivity and specificity of this prediction were 85.0% and 79.6%, respectively. Conclusions:The expression of miR-24-3p is high in the peripheral blood of patients with DN, and its level is associated with blood glucose metabolism, blood magnesium level, renal inflammatory injury, renal interstitial fibrosis, and glomerular atrophy. Upregulation of miR-24-3p may increase the risk of renal disease and exacerbate kidney damage in patients with T2DM.

Objective To investigate the protective effect of umbilical cord mesenchymal stem cells (UCMSCs) on traumatic brain injury (TBI) in rats.Methods Thirty healthy Sprague-Dawley rats (10 rats for each group) were randomly divided into normal control group (normal),model group (injection of saline after TBI) and UCMSCs transplantation group (injection of UCMSCs after TBI).The rats in experimental groups were sacrificed on the 10th day after UCMSCs transplantation.The percentage of UCMSCs in brain tissue was detected by flow cytometry.The pathological changes of brain tissue were observed by hematoxylin-eosin (HE) staining method.The expressions of vascular endothelial growth factor (VEGF),glial fibrillary acidic protein (GFAP) and brain-derived neurotrophic factor (BDNF) in brain tissue were measured by immunohistochemistry and immunofluorescence double staining.The neurological deficit was evaluated by neurological deficit degree.Results The percentage of CD90,CD73 and CD105 cells in the UCMSCs transplantation group was significandtly higher than that in the model group (0.4％ vs 0.1％,P＜0.05).The results of HE staining showed that the brain injury of the transplanted group was alleviated compared with the model group (P＜0.05).The VEGF of the brain tissue in injury area in the UCMSCs transplantation group was higher than that in the model group (P＜0.05).The number of GFAP and BDNF positive cells in the UCMSCs transplantation group was higher than that in the model group (P＜0.05),and the neurological deficit score was also higher than that in the model group (P＜0.05).Conclusions UCMSCs transplantation for the treatment of TBI rats can effectively reduce the vascular damage in the injury area and promote nerve recovery.