Objective To explore the genes related to the pathogenesis of polycystic ovary syndrome(PCOS)through bioinformatics methods and verify them in ovarian granulosa cells,providing a reference for screening potential molecular markers of PCOS.Methods The GSE34526 and GSE137684 datasets were re-spectively used as the training set and validation set.The inflammation-related genes potentially associated with the onset of PCOS were explored through differential expression analysis and weighted gene co-expres-sion network analysis.Subsequently,their predictive value was verified through the receiver operating charac-teristic(ROC)curve.Thirty patients with PCOS(the PCOS group)and 27 patients without PCOS(the con-trol group)who were treated in Affiliated Hospital of Youjiang Medical University for Nationalities were se-lected as the research objects.RT-qPCR was applied to verify the expression level of core genes in granular cells.Spearman correlation and multiple linear regression were used to analyze the correlation between the ex-pression level of core genes and various clinical parameters,and the ROC curve was used to analyze the diag-nostic efficacy of the expression level of core genes for PCOS.Finally,the protein expression level of core genes was verified on animal models.Results A total of 1 888 differentially expressed genes,89 module-relat-ed genes and 366 inflammatory response-related genes were identified.The core gene phosphatidylinositol-4,5-diphosphate 3-kinase catalytic subunit γ(PIK3CG)was ultimately obtained by taking co-expressed genes and verifying with the ROC curve.RT-qPCR results showed that the expression level of PIK3CG in granulosa cells of the PCOS group was higher than that of the control group(P<0.05).Spearman correlation analysis showed that the expression level of PIK3CG was positively correlated with BMI,testosterone(T),fasting in-sulin(FINS),and insulin resistance index(HOMA-IR,P<0.05).Multivariate linear regression analysis showed that FINS,HOMA-IR and BMI were increased with the increasing of PIK3CG expression level(P<0.05).ROC curve analysis showed that the area under the curve for diagnosing PCOS patients with PIK3CG mRNA expression levels in granulosa cells was 0.659 3.The immunohistochemical results showed that the ex-pression of PIK3CG protein in the ovarian tissues of mice in the PCOS group was significantly increased.Con-clusion PIK3CG may be potentially associated with the pathogenesis of PCOS.

Objective To explore the efficiency of training of professional nurses.Methods A total of 31 nurses were enrolled in a 12-week training of a comprehensive and systematic teaching practice containing theory teaching,skills training and clinical practice 3 domains between September and November in 2014.Nurses′nursing theory on specialized nursing,specialized nursing skills before and after the training were compared.Results After training,the accumulated score and item score of trainees on 50 knowledge points of burn specialized nursing theory, such as burn ward management system,monitoring skills on critically ill patients,perioperative skills,medication knowledge on specific treatment,explanation of common inspection and examination results increased compared to those before the training(P <0.05).Total and each item score of 3 skills operation assessment (body position change using turn-over bed,suspension bed application,cardiopulmonary resuscitation)of trainees after training were higher than those before training(P <0.05).Total score of self-growth evaluation and each item of 4 programs (nursing theory,basic nursing skills,intensive care level,nursing competence)of the trainees were higher than those before training(P <0.05).Conclusions Systematic and comprehensive theory teaching and clinical practice training can increase the nurses′level of professional theory and skills.Scientific and practical training content combined with training form close to clinic can effectively raise their comprehensive ability in nursing practice.