Hearing loss is one of the most prevalent sensory disorders affecting the human nervous system. Liquid-liquid phase separation (LLPS) is a physiological process that facilitates the reversible and dynamic assembly of biomolecular condensates. Increasing evidence suggests that LLPS plays a significant role in the pathogenesis of hereditary hearing loss. Nevertheless, there is a conspicuous lack of systematic investigations exploring the impact of LLPS abnormalities on the etiology of hereditary hearing loss. In this review, we examine the mechanisms by which dysfunctions in LLPS contribute to hereditary hearing loss, specifically focusing on its effects on mechanoelectrical transduction in hair bundles, transcriptional regulation, post-transcriptional modifications, the actin cytoskeleton, ion homeostasis within the inner ear, and energy and redox homeostasis. Furthermore, we evaluate the considerable potential of targeting LLPS as a therapeutic approach for hearing loss and propose innovative perspectives on LLPS that may guide future research initiatives in the field of auditory disorders.
Humans ; Animals ; Hearing Loss/physiopathology* ; Phase Separation

Objective To design web-based simulated training system for skills and clinical nursing thinking for the independent training of vocational nursing students, and analyze the training effect. Methods Nursing students from five sections in Suzhou Vocationul Health College were chosen randomly as the research object from October to November 2015, and a web-based training program which involved three categories and eight skills was designed and was employed to train the students, then a questionnaire was distributed to them and the results were analyzed.Results Nursing students′recognition degree to the 14 kinds of ability that should be paid attention in the adults nursing was 24.4%-74.7%, and their recognition degree to the 14 kinds of ability in this training system was 17.3%-69.3%. More than 90% of the nursing students thought that the design of this training system was creative, the design of training questions was rational, and the contents were scientific and advanced with high quality. However, 37. 3% of nursing students thought that the number of training programs was general/too small. About 90% of nursing students thought that this training system was helpfull in improving their ability and their study. The average scores of 223 nursing students in inquiry, physical examination, and nursing for patients with heart failure, coronary heart disease and diabetics were (84.4±17.9), (84.7±15.9), (84.7±13.5), (85.3±12.9) and (86.1±12.4).Conclusions The web-based simulated training system for skill and clinical nursing thinking has a good assist-teaching functiom, and it provides an access for nursing students to practice in their spare time. It was an innovative teaching practice with advancement and scientificity and it can help to improve students′skills and ability in clinical thinking.

BACKGROUND:At present, there are stil differences in the studies of total-etch, self-etch and self-bonding resin cement effect on the coronal microleakage and bonding strength of fiber posts.
OBJECTIVE:To evaluate the coronal microleakage and the bonding strength of fiber posts treated with three kinds of resin cements.
METHODS:Total y 32 upper incisors were randomly divided into five groups, including three experimental groups and two control groups. After the root canal preparation, three kinds of resin cements (EMBRACE WetBond, LuxaCore, Medental Multi-cure) were used to fiber posts with the bond diameter of 1.4 mm. Stereomicroscope was used to observe the microleakage. Then, the specimens were cut into 2 mm wafer along the axis of tooth, and universal testing machine for push-out test was used to observe the failure mode. In the positive control group, no root canal preparation was done, the root was coated with nail polish, and the crown was directly exposed to the dye. In the negative control group, no root canal preparation was done, the root canal orifice was covered with the resin, the tooth was overal coated with nail polish and then embedded 1 mm below the section.
RESULTS AND CONCLUSION:The microleakage was observed in al the three resin cements, Medental Multi-cure showed the least microleakage and LuxaCore showed the largest microleakage, and there was significantly different among the three kinds of resin cements (P<0.05). The bonding strength of three cements had significant differences (P<0.05), and ranked from high to low:Mdental Multi-cure, LuxaCore, and EMBRACE WetBond. The main fracture modes were binder/fiber post fracture and mixed failure. The results suggest that the total-etch resin cement binds tightly with the dentin, and owns a superiority in the microleakage and bonding property as compared with the self-etch resin cements and self-bonding resin cements.