Research has confirmed that compared to traditional room temperature-stored platelets [RTP, (22±2) ℃], cold-stored platelets (CSP, 1-6℃) extend the stored time by inhibiting bacterial proliferation, representing an important strategy to break through the bottleneck of platelet storage. However, the low temperature environment induces significant changes in platelet morphology, metabolism and function. The morphological changes and surface marker changes together lead to functional impairment, which in turn affect its hemostatic efficacy. In recent years, research on metabolomics, apoptosis mechanisms, and innovative preservation strategies and technologies has provided new perspectives for intensive analysis of the damage mechanisms of CSP. This review summarizes the changes in CSP performance, active components, and damage mechanisms, explores the research progress in preservation solutions and methods, and combines them with the current clinical application status. The aim is to provide a theoretical basis for intensive analysis of CSP performance changes and optimization of platelet storage strategies, and promote the application of CSP based on its functional characteristics in emergency treatment of active bleeding patients in China.

Objective:To establish a quality control method for the standard decoction of Polygoni Avicularis Herba.Methods:Totally 12 batches of decoction pieces from different origins were collected, the standard decoction was prepared and the quality evaluation method was established, the content of index components in the decoction pieces and the standard decoction was determined with HPLC, the index components, solution pH and other parameters were calculated, and the similarity analysis was carried out against the fingerprints.Results:The total content of myricetin in 12 batches of decoction pieces was >0.12%, and the content of myricetin in the standard decoction was >0.03%, which met the standard of the 2020 edition of the Chinese Pharmacopoeia. The pH value was 5.1-5.5, the transfer rate of myricetin components ranged from 50.0%-106.3%, and the fingerprint study showed that there were 7 common peaks. The similarity analysis results indicated that the standard decoction of 12 batches of decoction pieces of Polygoni Avicularis Herba had good consistency.Conclusion:The preparation process is stable and feasible in line with the traditional decoction preparation method, and can be used for the research and quality evaluation of the standard decoction.

The walking blood bank (WBB) is a pool of donors available "on call" to donate whole blood (WB) in emergency situations. It can provide sufficient blood resources in a timely manner during shortages, and can simultaneously meet the demand for various blood components such as red blood cells, platelets and plasma. Currently, WBB has been implemented in both military and/or civilian contexts in many Western countries, with satisfactory outcomes. This article summarizes the necessity of WBB, the screening of blood donors, management and maintenance, as well as logistics and other situations, in order to provide certain references for the establishment and development of WBB in China.

Objective To analyze the influencing factors for carotid plaque formation in patients with essential hypertension(EH)with LASSO-Cox regression.Methods A retrospective analysis was conducted on 325 patients with new-onset hypertension admitted to the First Affiliated Hos-pital of Zhengzhou University from March 2020 to March 2021.Among them,308 completed a fol-low-up of 2-year,and then according to carotid plaque occurred or not(the carotid intima-media thickness≥1.5 mm),they were divided into in the occurrence group(85 cases)and non-occur-rence group(223 cases).Clinical data and circular RNA expression level were compared between the two groups.LASSO regression model was used to screen out the predictors of carotid plaque formation,and Cox regression model was employed to explore the influencing factors of the for-mation.Results The occurrence group had significantly advanced age,higher body mass index and CIMT,larger proportion of diabetes mellitus,and elevated serum uric acid and homocysteine(Hey)levels,but lower high-density lipoprotein cholesterol(HDL-C)than the non-occurrence group(P＜0.01).In addition,the expression levels of has-circ-0105130,has-circ-0109569,has-circ-0072659,has-circ-0079586 and has-circ-0064684 were obviously higher in the occurrence group than the non-occurrence group(P＜0.01).Multivariate Cox regression analysis showed that has-circ-0109569 and Hcy were independent risk factors for carotid plaque formation(P＜0.05,P＜0.01),and HDL-C was an independent protective factor(P＜0.01).The AUC value of for the combination of has-circ-0109569,HDL-C and Hcy in prediction of carotid artery plaques was 0.977(95％CI:0.953-0.991,P＜0.01).Conclusion High has-circ-0109569 and Hcy and low HDL-C levels are risk factors for carotid plaque formation in the EH patients.

Pancreatic duct stents are essential devices for managing chronic pancreatitis,ductal strictures,and postoperative fistula.Conventional plastic and metal stents effectively facilitate pancreatic drainage but often cause infection,restenosis,or migration upon long-term implantation.An ideal stent should provide excellent biocompatibility,efficient drainage,and controllable biodegradation.With advances in material science and medical-engineering integration,stent technology has evolved from inert implantation to intelligent degradation.Biodegradable polymers and metals,particularly magnesium alloys(Mg-Zn-Mn),offer tunable mechanical strength,corrosion resistance,and in vivo degradability.Mg-2Zn-1.0Mn alloy achieves balanced strength and corrosion control through compositional optimization and surface modification.Polymeric stents such as polylactic acid and polydioxanone demonstrate favorable drainage and avoid secondary removal.Composite biodegradable stents,exemplified by the multi-rate ARCHIMEDES model,have received international approval.Supported by 3D printing and smart functionalization-such as drug-eluting or shape-memory designs-next-generation pancreatic stents may achieve integrated functions of support,repair,and tumor inhibition.Future research should emphasize interdisciplinary material design,degradation kinetics under physiological conditions,and long-term biocompatibility to accelerate clinical translation.

Pancreatic duct stents are essential devices for managing chronic pancreatitis,ductal strictures,and postoperative fistula.Conventional plastic and metal stents effectively facilitate pancreatic drainage but often cause infection,restenosis,or migration upon long-term implantation.An ideal stent should provide excellent biocompatibility,efficient drainage,and controllable biodegradation.With advances in material science and medical-engineering integration,stent technology has evolved from inert implantation to intelligent degradation.Biodegradable polymers and metals,particularly magnesium alloys(Mg-Zn-Mn),offer tunable mechanical strength,corrosion resistance,and in vivo degradability.Mg-2Zn-1.0Mn alloy achieves balanced strength and corrosion control through compositional optimization and surface modification.Polymeric stents such as polylactic acid and polydioxanone demonstrate favorable drainage and avoid secondary removal.Composite biodegradable stents,exemplified by the multi-rate ARCHIMEDES model,have received international approval.Supported by 3D printing and smart functionalization-such as drug-eluting or shape-memory designs-next-generation pancreatic stents may achieve integrated functions of support,repair,and tumor inhibition.Future research should emphasize interdisciplinary material design,degradation kinetics under physiological conditions,and long-term biocompatibility to accelerate clinical translation.

Objective To evaluate the efficacy and safety of Qixian Tongluo Formula in the treatment of post-cerebral infarction paralysis with kidney deficiency and blood stasis syndrome,and to preliminarily explore the molecular mechanism of Qixian Tongluo Formula in improving impaired motor function from the perspective of cross-kingdom regulation of Chinese medicine microRNA(miRNA).Methods A pragmatic randomized controlled trial was conducted with 102 patients in the recovery period of post-cerebral infarction paralysis with kidney deficiency and blood stasis syndrome in our hospital.Patients were randomly divided into trial group and control group,with 51 cases in each group.The control group received standard Western medicine standard treatment,while the trial group received Qixian Tongluo Formula in addition to the standard treatment,with one dose per day,boiled in water,and taken warm after breakfast and dinner for a course of 2 months.The disability rate was used as the main efficacy indicator,and the incidence of adverse reactions was used as a safety indicator.miRNA from patient serum and Qixian Tongluo decoction were extracted respectively,and high-throughput sequencing was performed.The two sequences were compared to screen out the cross-kingdom gene transfer of Chinese medicine miRNA.Finally,its target genes of miRNA were predicted,and GO function and KEGG pathway enrichment analysis were carried out.Results A total of 67 patients completed the clinical trial,including 36 cases in the trial group and 31 cases in the control group;The disability rate in the trial group(13.9%)was lower than that in the control group(35.5%)(P<0.05);The incidence of adverse reactions was similar between the trial group(7.69%)and the control group(6.06%)(P>0.05);A total of 9530 Qixian Tongluo decoction miRNA sequences were screened,with 150 potentially involved in cross-kingdom gene transfer,including families such as miR-15 and miR-17;According to the target gene prediction of the top 10 miRNAs in cross-kingdom gene transfer of Chinese medicine,345 overlapping target genes were obtained;GO functional enrichment analysis revealed 16 biological processes,7 cellular components,and 2 molecular functions among the top 25 enriched functions,while KEGG pathway analysis mainly focused on the transforming growth factor-βsignaling pathway,neurotrophin signaling pathway,which are closely related to neural repair and functional recovery processes such as glial scar formation and synaptic plasticity after cerebral ischemia.Conclusion Qixian Tongluo Formula can significantly improve the functional independence level of patients with kidney deficiency and blood stasis syndrome in the recovery period of paralysis after cerebral infarction,offering a safe and effective treatment option for these patients;There were a large number of miRNAs in Qixian Tongluo decoction,some of which could cross-kingdom transferred into the human blood circulation,and promote the recovery of motor function in patients with cerebral infarction through multi-target,multi link and multi pathway gene network regulation.This study provides a new idea for subsequent clinical and basic research.

Objective To evaluate the efficacy and safety of Qixian Tongluo Formula in the treatment of post-cerebral infarction paralysis with kidney deficiency and blood stasis syndrome,and to preliminarily explore the molecular mechanism of Qixian Tongluo Formula in improving impaired motor function from the perspective of cross-kingdom regulation of Chinese medicine microRNA(miRNA).Methods A pragmatic randomized controlled trial was conducted with 102 patients in the recovery period of post-cerebral infarction paralysis with kidney deficiency and blood stasis syndrome in our hospital.Patients were randomly divided into trial group and control group,with 51 cases in each group.The control group received standard Western medicine standard treatment,while the trial group received Qixian Tongluo Formula in addition to the standard treatment,with one dose per day,boiled in water,and taken warm after breakfast and dinner for a course of 2 months.The disability rate was used as the main efficacy indicator,and the incidence of adverse reactions was used as a safety indicator.miRNA from patient serum and Qixian Tongluo decoction were extracted respectively,and high-throughput sequencing was performed.The two sequences were compared to screen out the cross-kingdom gene transfer of Chinese medicine miRNA.Finally,its target genes of miRNA were predicted,and GO function and KEGG pathway enrichment analysis were carried out.Results A total of 67 patients completed the clinical trial,including 36 cases in the trial group and 31 cases in the control group;The disability rate in the trial group(13.9%)was lower than that in the control group(35.5%)(P<0.05);The incidence of adverse reactions was similar between the trial group(7.69%)and the control group(6.06%)(P>0.05);A total of 9530 Qixian Tongluo decoction miRNA sequences were screened,with 150 potentially involved in cross-kingdom gene transfer,including families such as miR-15 and miR-17;According to the target gene prediction of the top 10 miRNAs in cross-kingdom gene transfer of Chinese medicine,345 overlapping target genes were obtained;GO functional enrichment analysis revealed 16 biological processes,7 cellular components,and 2 molecular functions among the top 25 enriched functions,while KEGG pathway analysis mainly focused on the transforming growth factor-βsignaling pathway,neurotrophin signaling pathway,which are closely related to neural repair and functional recovery processes such as glial scar formation and synaptic plasticity after cerebral ischemia.Conclusion Qixian Tongluo Formula can significantly improve the functional independence level of patients with kidney deficiency and blood stasis syndrome in the recovery period of paralysis after cerebral infarction,offering a safe and effective treatment option for these patients;There were a large number of miRNAs in Qixian Tongluo decoction,some of which could cross-kingdom transferred into the human blood circulation,and promote the recovery of motor function in patients with cerebral infarction through multi-target,multi link and multi pathway gene network regulation.This study provides a new idea for subsequent clinical and basic research.

Objective To analyze the influencing factors for carotid plaque formation in patients with essential hypertension(EH)with LASSO-Cox regression.Methods A retrospective analysis was conducted on 325 patients with new-onset hypertension admitted to the First Affiliated Hos-pital of Zhengzhou University from March 2020 to March 2021.Among them,308 completed a fol-low-up of 2-year,and then according to carotid plaque occurred or not(the carotid intima-media thickness≥1.5 mm),they were divided into in the occurrence group(85 cases)and non-occur-rence group(223 cases).Clinical data and circular RNA expression level were compared between the two groups.LASSO regression model was used to screen out the predictors of carotid plaque formation,and Cox regression model was employed to explore the influencing factors of the for-mation.Results The occurrence group had significantly advanced age,higher body mass index and CIMT,larger proportion of diabetes mellitus,and elevated serum uric acid and homocysteine(Hey)levels,but lower high-density lipoprotein cholesterol(HDL-C)than the non-occurrence group(P＜0.01).In addition,the expression levels of has-circ-0105130,has-circ-0109569,has-circ-0072659,has-circ-0079586 and has-circ-0064684 were obviously higher in the occurrence group than the non-occurrence group(P＜0.01).Multivariate Cox regression analysis showed that has-circ-0109569 and Hcy were independent risk factors for carotid plaque formation(P＜0.05,P＜0.01),and HDL-C was an independent protective factor(P＜0.01).The AUC value of for the combination of has-circ-0109569,HDL-C and Hcy in prediction of carotid artery plaques was 0.977(95％CI:0.953-0.991,P＜0.01).Conclusion High has-circ-0109569 and Hcy and low HDL-C levels are risk factors for carotid plaque formation in the EH patients.

BACKGROUND:Compared with traditional two-dimensional culture,three-dimensional microtissue culture can show greater advantages.However,more favorable cultivation methods in three-dimensional culture still need to be further explored. OBJECTIVE:To evaluate the cell behavior of microtissue and its ability to promote cartilage formation under two three-dimensional culture methods. METHODS:Cartilage-derived microcarriers were prepared by chemical decellularization and tissue crushing.DNA quantification and nuclear staining were used to verify the success of decellularization,and histological staining was used to observe the matrix retention before and after decellularization.The microcarriers were characterized by scanning electron microscopy and CCK-8 assay.Cartilage-derived microtissues were constructed by combining cartilage-derived microcarriers with human adipose mesenchymal stem cells through three-dimensional static culture and three-dimensional dynamic culture methods.The cell viability and chondrogenic ability of the two groups of microtissues were detected by scanning electron microscopy,live and dead staining,and RT-qPCR. RESULTS AND CONCLUSION:(1)Cartilage-derived microcarriers were successfully prepared.Compared with before decellularization,the DNA content significantly decreased after decellularization(P<0.001).Scanning electron microscope observation showed that the surface of the microcarrier was surrounded by collagen,maintaining the characteristics of the natural extracellular matrix of cartilage cells.CCK-8 assay indicated that microcarriers had no cytotoxicity and could promote cell proliferation.(2)Scanning electron microscopy and live and dead staining results showed that compared with the three-dimensional static group,the three-dimensional dynamic group had a more extended morphology of microtissue cells,and extensive connections between cells and cells,between cells and matrix,and between matrix.(3)The results of RT-qPCR showed that the expressions of SOX9,proteoglycan,and type Ⅱ collagen in microtissues of both groups were increased at 7 or 14 days.The relative expression levels of each gene in the three-dimensional dynamic group were significantly higher than those in the three-dimensional static group at 14 days(P<0.05).At 21 days,the three-dimensional static group had significantly higher gene expression compared with the three-diomensional dynamic group(P<0.001).(4)The results showed that compared with three-dimensional static culture microtissue,three-dimensional dynamic culture microtissue could achieve higher expression of chondrogen-related genes in a shorter time,showing better cell viability and chondrogenic ability.