With the introduction and implementation of relevant policies such as the outline of "Healthy China 2030" plan and Healthy China Action (2019-2030), the health service function of traditional Chinese medicine (TCM) has been valued and developed. TCM health literacy has become one of the important ways to promote the health of the whole population. TCM health preservation literacy refers to the ability of individuals to obtain TCM health preservation concepts, knowledge and technical services, and to make correct health care decisions to promote their own health by using knowledge and information as well as medical and technical services. According to survey data from the National Administration of Traditional Chinese Medicine, the compliance rate of TCM health literacy among Chinese citizens has increased from 8.55% in 2014 to 24.62% in 2023, and is still on the rise. However, there are differences in the level of TCM health literacy among different regions, genders, and occupational groups. This article retrieves relevant literature published on CNKI and Wanfang Data from the establishment of the databases until December 2024. The content is sorted out using thematic analysis, and a review is conducted on the concept, evaluation tools, current status, and influencing factors of TCM health literacy. This article provides a reference for enhancing public health awareness and improving the level of TCM health literacy.

Autism spectrum disorder(ASD),characterized by unknown etiology and high heterogeneity,ne-cessitates precise diagnostic and intervention strategies.Neuroimaging techniques have shown great promise in un-covering the neural mechanisms of ASD,providing a foundation for aided diagnosis and transcranial magnetic stim-ulation(TMS)interventions.This review highlights that integrating multimodal neuroimaging and developing indi-vidualized indices with developmental specificity can significantly improve the accuracy of ASD diagnosis and clas-sification.Furthermore,TMS interventions guided by functional connectivity derived from functional magnetic reso-nance imaging(fMRI)offer a personalized approach to ASD treatment.

Objective To clarify the active ingredients and the potential molecular mechanism of Guben Qushi Huayu Prescription in treating psoriasis recurrence.Methods An ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS)was applied to analyze the whole formula and the constituents absorbed into blood of Guben Qushi Huayu Prescription,and molecular docking technology was used to study the binding affinity of the constituents absorbed into blood with psoriasis-related immunomodulatory proteins such as CD69 and CD103 proteins.Results Mass spectrometry analysis identified 21 active ingredients such as paeoniflorin in Guben Qushi Huayu Prescription,including several known anti-inflammatory and immunomodulatory compounds.Analysis of the constituents absorbed into blood identified 11 ingredients,including paeoniflorin,that may affect the course of psoriasis through blood circulation.Molecular docking studies revealed that the constituents absorbed into blood,including astilbin,isoastilbin,chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,helicine,paeoniflorin,ononin,all had high binding affinities with CD69 and CD103 proteins.Conclusion This research reveals the main active ingredients of Guben Qushi Huayu Prescription and their potential mechanism for regulating the recurrence of psoriasis by mass spectrometry and molecular docking technology,contributing to providing scientific basis for further pharmacological research and clinical application.

Objective To explore the prenatal ultrasound imaging features of Tessier number 7 cleft.Methods From Janu-ary 2018 to June2024,8 cases of Tessier number 7 cleft were diagnosed in Maternal and Child Health Hospital of Hubei Prov-ince.Theprenatal ultrasound images were analyzed and compared with pathology result after induced labor or surgery after birth.Results There were 8 cases of Tessier number 7 cleft,among which 7 cases were singletons and 1 was one of twins.Prenatal ultrasound findings:5 cases were diagnosed by prenatal ultrasound at 23 to 27+5 gestational weeks.One case of bilateral ear anomaly,1 case of unilateral ear anomaly,2 cases of bilateral accessory auricle,3 cases of unilateral accessory auri-cle,1 case of facial cheek mass,and 1 case of micrognathia were found through two-dimensional combined with three-dimension-al prenatal ultrasound.Other associated malformations:2 cases of single umbilical artery,1 case of congenital vascular ring.One case was not diagnosed by prenatal ultrasound in other hospital;2 cases were not diagnosed in our hospital,including the twin case whose facial features weredifficult to indentify due to oligohydramnios.Pathological or surgical results:therewere 4 female cases and 4 male cases.Transverse facial fissures occurred on the right side in 6 cases and on the left side in 2 cases.Transverse facial fissures were rated as GradeⅠin 3 cases and GradeⅡin 5 cases.There were combined bilateral ear anomalies in 4 cases,u-nilateral ear anomalies in 3 cases,bilateral ear canal atresia in 1 case,unilateral ear canal atresia in 3 cases;bilateral accessory au-ricle in 3 cases,unilateral accessory auricle in 5 cases,facial cheek mass in 3 cases,and facial muscle depression in 3 ca-ses.Prenatal ultrasound findings wereconfirmed by pathological results.One case of median palatalcleft,one of twin cases with upper limb joint movement restrictions,and anal atresia were additionally found in pathological examination.Conclusion The location and size of facial transverse clefts,ear anomalies,accessory auricle and micrognathia can be observed by two-dimensional ultrasound.Three-dimensional ultrasound surface rendering improves the accuracy of diagnosis,and combined application is an important method for diagnosis of Tessier number 7 cleft in the fetal period.

Autism spectrum disorder(ASD),characterized by unknown etiology and high heterogeneity,ne-cessitates precise diagnostic and intervention strategies.Neuroimaging techniques have shown great promise in un-covering the neural mechanisms of ASD,providing a foundation for aided diagnosis and transcranial magnetic stim-ulation(TMS)interventions.This review highlights that integrating multimodal neuroimaging and developing indi-vidualized indices with developmental specificity can significantly improve the accuracy of ASD diagnosis and clas-sification.Furthermore,TMS interventions guided by functional connectivity derived from functional magnetic reso-nance imaging(fMRI)offer a personalized approach to ASD treatment.

Objective To explore the prenatal ultrasound imaging features of Tessier number 7 cleft.Methods From Janu-ary 2018 to June2024,8 cases of Tessier number 7 cleft were diagnosed in Maternal and Child Health Hospital of Hubei Prov-ince.Theprenatal ultrasound images were analyzed and compared with pathology result after induced labor or surgery after birth.Results There were 8 cases of Tessier number 7 cleft,among which 7 cases were singletons and 1 was one of twins.Prenatal ultrasound findings:5 cases were diagnosed by prenatal ultrasound at 23 to 27+5 gestational weeks.One case of bilateral ear anomaly,1 case of unilateral ear anomaly,2 cases of bilateral accessory auricle,3 cases of unilateral accessory auri-cle,1 case of facial cheek mass,and 1 case of micrognathia were found through two-dimensional combined with three-dimension-al prenatal ultrasound.Other associated malformations:2 cases of single umbilical artery,1 case of congenital vascular ring.One case was not diagnosed by prenatal ultrasound in other hospital;2 cases were not diagnosed in our hospital,including the twin case whose facial features weredifficult to indentify due to oligohydramnios.Pathological or surgical results:therewere 4 female cases and 4 male cases.Transverse facial fissures occurred on the right side in 6 cases and on the left side in 2 cases.Transverse facial fissures were rated as GradeⅠin 3 cases and GradeⅡin 5 cases.There were combined bilateral ear anomalies in 4 cases,u-nilateral ear anomalies in 3 cases,bilateral ear canal atresia in 1 case,unilateral ear canal atresia in 3 cases;bilateral accessory au-ricle in 3 cases,unilateral accessory auricle in 5 cases,facial cheek mass in 3 cases,and facial muscle depression in 3 ca-ses.Prenatal ultrasound findings wereconfirmed by pathological results.One case of median palatalcleft,one of twin cases with upper limb joint movement restrictions,and anal atresia were additionally found in pathological examination.Conclusion The location and size of facial transverse clefts,ear anomalies,accessory auricle and micrognathia can be observed by two-dimensional ultrasound.Three-dimensional ultrasound surface rendering improves the accuracy of diagnosis,and combined application is an important method for diagnosis of Tessier number 7 cleft in the fetal period.

Objective:To explore the clinical efficacy of free superficial circumflex iliac artery perforator flap (SCIAPF) in reconstruction of soft tissue defects of hand and foot in children.Methods:From August 2021 to August 2023, free SCIAPFs were used to reconstruct soft tissue defects of hands and feet in 8 children at the Department of Hand and Foot Surgery, Zhoukou Huaihai Hospital. There were 6 boys and 2 girls aged between 2 and 7 years old. The sites of soft tissue defect were: 1 of metacarpophalangeal joint in the functional area of left thumb, 2 of dorsal right hand, 1 of dorsal left foot, 2 of medial malleolus of right foot, and 2 of right forefoot, and all were accompanied with tendon and bone exposure. The size of soft tissue defects ranged from 2.5 cm×3.0 cm to 6.0 cm×3.5 cm. After emergency debridement, the children received flap transfers, the flaps sized from 3.0 cm×3.5 cm to 7.0 cm×4.0 cm. Donor sites were closed by running intradermal suture. All children were included in the scheduled postoperative follow-up by visit of outpatient clinic, WeChat interviews and home visits to observe the appearance, colour, texture and sensation of the flaps, the functional recovery of the affected hands and feet, as well as the healing of donor sites.Results:After surgery, all 8 flaps survived beside 1 had a partial necrosis at the edge and healed with a scab after dressing change. Flap donor sites healed in one stage. Scheduled postoperative follow-up lasted from 7 to 22 months, with an average of 10 months. The flaps presented a slightly bloated appearance, soft texture with a restored protective sensation and satisfactory functional recovery of hands and feet. Linear scars were left at the donor sites. According to the Evaluation Trial Standards of Upper Limb Partial Functional of Hand Surgery of Chinese Medical Association, 1 child was of excellent and 2 children of good in hand function of the 3 children with hand injury. The Maryland Foot Score revealed 3 children were of excellent and 2 of good in foot function of the 5 children with foot injury.Conclusion:Free SCIAPF is an ideal option for reconstruction of soft tissue defects of hand and foot in children, with concealed donor site, convenient flap harvesting, fewer complications and satisfactory reconstructive effect.

Objectives:To explore the risk factors for death from ruptured acute aortic dissection during emergency treatment,construct and validate a risk prediction model for death from ruptured acute aortic dissection during emergency treatment. Methods:A total of 301 cases of acute aortic dissection patients who were admitted to Chinese Academy of Medical Sciences Fuwai Hospital from January 2018 to August 2021 were included in this study.Patients were divided into survival subgroup(n=239)and death subgroup(n=62)according to whether dissection rupture occurred in the acute stage of the disease.Univariate and multivariate analyses were performed.Logistic regression analysis was used to establish the risk prediction model.The Hosmer-Lemeshow test was conducted to assess the model's goodness of fit,and the receiver operating characteristic curve(ROC curve)was used to evaluate the model's predictive performance.A prospective validation was performed on 129 cases of acute aortic dissection patients admitted to our hospital's emergency department from September 2021 to September 2022. Results:Among the 301 cases of acute aortic dissection patients,there were 62 cases of rupture and death,with an incidence rate of 20.6%.The results of multivariate analysis showed that age(OR=1.066,95%CI:1.034-1.099),type A dissection(OR=0.045,95%CI:0.006-0.364),history of hypertension(OR=0.377,95%CI:0.167-0.850),and concomitant hypotension(OR=4.424,95%CI:1.467-13.340)were determinants of deaths.The model formula was Z=-5.624+0.064×age-0.976×history of hypertension(yes=1,no=0)-3.104×type(Type A=0,Type B=1)+1.487×concomitant hypotension(yes=1,no=0).The Hosmer-Lemeshow test result showed χ2=9.328,df=8,P=0.315,the area under the ROC curve was 0.874,sensitivity was 79.0%,specificity was 81.6%,and the maximum Youden index was 0.606.The model validation result showed that the area under the ROC curve was 0.722,sensitivity was 73.7%,specificity was 69.1%,and accuracy was 89.9%. Conclusions:Age,history of hypertension,dissection type,and combined hypotension are predictors of the risk prediction model for death from dissection rupture in patients with acute aortic dissection during emergency treatment.The model constructed in this study has good predictive performance,which can provide reference for medical staffto quickly identify high-risk patients for death from ruptured acute aortic dissection and timely predictive measures could be highlighted in indicated cases.

Objective:To explore the clinical effect of using great toe fibular flaps of both feet on reconstruction of pulp defects of two neighbouring digits.Methods:A total of 14 digit pulp defects in 7 cases were repaired in Zhoukou Huaihai Hospital using great toe fibular flaps of both feet from August 2020 to January 2023. Of the 7 cases, there were 4 males and 3 females, with an average of 28 years old, ranging from 19 to 45 years old. Meanwhile, there were 4 cases in left hand and 3 cases in right hand. There were 3 cases of digit pulp defects in index and middle fingers, 2 in middle and ring fingers, and 2 in thumb and index fingers. The area of soft tissue defect in 1.2 cm×1.5 cm-3.0 cm×2.5 cm, and flap was 1.5 cm ×1.8 cm-3.2 cm×2.8 cm. Furthermore, 1 case underwent emergency surgery and 5 were repaired in elective surgery. The donor site of the flap was closed directly, and an intermediate-thickness skin graft was prepared from the medial plantar area for transfer in the case of high suture tension at the wound edge. After surgery, patients received postoperative by outpatient clinic and WeChat to observe the appearance, sensation, functional recovery and flap contracture of digits, as well as the movement of the great toes of both feet.Results:After the surgery, all flaps in the 7 cases survived smoothly and the donor sites healed. All patients entered scheduled follow-ups postoperatively for 6 months to 2 years, with an average of 9 months. The flap showed an aesthetic appearance and excellent sensation, with a TPD of 3-6 mm, and satisfactory digit function. The donor site of the great toe fibular flap left linear scars only, without abnormality in range of motion and gait in walking. In addition, there were 5 in excellent and 2 in good according to the Evaluation Trial Standards of Upper Limb Partial Functional of Hand Surgery of Chinese Medical Association.Conclusion:Application of great toe fibular flaps of both feet is an ideal option for the simultaneous repair of pulp defects of two neighbouring digits, which can achieve good reconstructive results.

Objective The aim is to utilize CRISPR/Cas9 gene editing technology to construct Dmd gene mutant mice with a point mutation in exon 23 of the Dmd gene. Subsequently, the phenotypic changes of the mice in muscles and immune systems are analyzed and verified, providing an evaluation model for Duchenne muscular dystrophy and other related diseases.MethodsBased on the sequence characteristics of exon 23 of the Dmd gene, small guide RNA (sgRNA) was designed and synthesized. Cas9 mRNA, sgRNA fragments, and oligo donor DNA were microinjected into fertilized eggs of C57BL/6J mice. After transferring the fertilized eggs to surrogate mice, F0 generation mice were born. After mating with F0 generation mice, offspring mice were obtained, and Dmd gene positive mutant (Dmd^Mu/+) mice were obtained after genotype identification. Male hemizygous Dmd^Mu/+(Dmd^Mu/Y) mice were selected for phenotype validation. The body weight of live 3- and 9-month-old mice were recorded. Muscle tension was evaluated through the grid test. Hearts and semitendinosus muscles were collected, and the histopathological changes were observed using HE staining. Further, the expression of Dmd protein in muscle tissue of 9-month-old mice was analyzed by Western blotting.An acute inflammation model was established in Dmd^Mu/Y mice using lipopolysaccharide induction. Peripheral blood from the submandibular vein was collected, and the changes in the proportion of neutrophils and monocytes were detected by flow cytometry.Results The results of genome sequencing and Western blotting confirmed the successful construction of Dmd gene point mutant mice (Dmd^Mu/+ mice). Dmd protein expression was not detected in skeletal muscle and myocardium of Dmd^Mu/+ mice, and it was significantly reduced compared to wild-type C57BL/6J mice (P<0.05). Compared with wild-type mice of the same background, Dmd^Mu/Y mice at 3 and 9 months of age showed significant weight loss (P<0.01) and decreased muscle tension (P<0.05). 9-month-old Dmd^Mu/Y mice exhibited significant pathological changes in skeletal muscle and myocardium, including widening of intermuscular space. Under normal condition, compared with wild-type mice, the proportion of neutrophils and monocytes in the peripheral blood of 3-month-old Dmd^Mu/Y mice was significantly lower than that of wild-type mice (P<0.01). After lipopolysaccharide stimulation, the proportion of neutrophils in peripheral blood of 3-month-old Dmd^Mu/Y mice remained significantly lower compared to that of wild-type mice (P<0.01). The proportion of neutrophils in peripheral blood of 9-month-old Dmd^Mu/Y mice significantly decreased after lipopolysaccharide induction (P<0.01), with a trend of change observed in monocytes between groups.Conclusion The successful construction of the Dmd gene mutant mouse model has confirmed the vital function of Dmd gene in maintaining normal muscle tissue morphology and muscle tone. It preliminarily indicated that Dmd gene deletion could significantly reduce the proportion of neutrophils in peripheral blood, offering a new perspective for the study of immune system alterations in Duchenne muscular dystrophy patients.