We analyzed the epidemiological features and spatial distribution characteristics of human brucellosis in Jining city from 2014 to 2023,to provide a reference for further development of targeted prevention and control strategies and measures.Descrip-tive epidemiological methods were used to analyze the epidemiological characteristics of brucellosis cases in Jining from 2014 to 2023.The spatial regional correlation of brucellosis incidence in Jining and the clustering patterns of local areas were studied through spatial autocorrelation analysis with townships as the basic unit.A total of 3 520 cases of brucellosis were reported in Jining from 2014 to 2023,and the average annual incidence rate was 4.23/100 000,thus indicating a fluctuating trend overall.Reported cases peaked from March to August,and a sex ratio of 2.71 males to 1 female was observed.The 40-59 year age group had the most reported cases(50.39％).The incidence of brucellosis in Jining showed an imbalanced spatial distribution.Brucellosis incidence showed a spatially clustered distribution(Moran's I>0,P<0.05).Hotspots were distributed primarily in Sishui,Qufu,and Zoucheng.A total of one class Ⅰ clustering area and one class Ⅱ clustering area were detected in the spatial and temporal scans,and were located in Sishui,Qufu,and Liangshan county.After pathogenic AMOS-PCR typing analysis,64 Brucella isolates collected from Jinan City from 2022 to 2024 were all of the sheep strain,and sheep biovar 3 was predominant(70.31％).In 2014-2023,although Jining City experienced a high incidence of brucellosis,a downward trend was observed.Brucellosis showed a spatial clustering pattern concentrated in the northeastern region.Therefore,awareness and education must be strengthened among brucellosis practitioners in cluster areas,to en-hance case surveillance,improve the level of protection,and achieve early detection and treatment.

We analyzed the epidemiological features and spatial distribution characteristics of human brucellosis in Jining city from 2014 to 2023,to provide a reference for further development of targeted prevention and control strategies and measures.Descrip-tive epidemiological methods were used to analyze the epidemiological characteristics of brucellosis cases in Jining from 2014 to 2023.The spatial regional correlation of brucellosis incidence in Jining and the clustering patterns of local areas were studied through spatial autocorrelation analysis with townships as the basic unit.A total of 3 520 cases of brucellosis were reported in Jining from 2014 to 2023,and the average annual incidence rate was 4.23/100 000,thus indicating a fluctuating trend overall.Reported cases peaked from March to August,and a sex ratio of 2.71 males to 1 female was observed.The 40-59 year age group had the most reported cases(50.39％).The incidence of brucellosis in Jining showed an imbalanced spatial distribution.Brucellosis incidence showed a spatially clustered distribution(Moran's I>0,P<0.05).Hotspots were distributed primarily in Sishui,Qufu,and Zoucheng.A total of one class Ⅰ clustering area and one class Ⅱ clustering area were detected in the spatial and temporal scans,and were located in Sishui,Qufu,and Liangshan county.After pathogenic AMOS-PCR typing analysis,64 Brucella isolates collected from Jinan City from 2022 to 2024 were all of the sheep strain,and sheep biovar 3 was predominant(70.31％).In 2014-2023,although Jining City experienced a high incidence of brucellosis,a downward trend was observed.Brucellosis showed a spatial clustering pattern concentrated in the northeastern region.Therefore,awareness and education must be strengthened among brucellosis practitioners in cluster areas,to en-hance case surveillance,improve the level of protection,and achieve early detection and treatment.

Traditional Chinese medicine （TCM） has special advantages and independent value in fighting major epidemics such as emerging infectious diseases. To exert the efficacy of TCM in the field of public health emergency management， it is necessary to clarify the necessity and analyze the obstacles， hidden dangers， and ethical risks of integrating TCM into the public health emergency management system. By optimizing the legal system of integrating TCM into the public health emergency management system， improving the construction of system and mechanism of health emergency management for preventing and controlling major epidemics with TCM， enhancing the strength of clinical scientific research technology of TCM in preventing and controlling epidemics， strengthening the discipline education of TCM and the construction of the talent team， based on the background of combining Chinese and Western medicine， the integration of TCM into the national public health emergency management system can be promoted.

【Objective】 To evaluate the clinical efficacy and safety of platelet-rich plasma(PRP) in acute achilles tendon injury by meta-analysis. 【Methods】 Literature on clinical randomized controlled trial of PRP in the treatment of acute achilles tendon injury from Wanfang database, CNKI, VIP database, The Chinese Biological Literature Database, The Chinese Clinical Trials Registry, PubMed, Embase, Cochrane and The US Clinical Trials Registry as of August 2023 were retrieved. The control group received conventional treatment for acute achilles tendon injury, while PRP treatment group received additional PRP treatment. The primary outcome measure was visual analogue pain scale, and the secondary outcome measures were the achilles tendon fracture score, maximum heel rise height, calf circumference and ankle range of motion. The quality of the literature was assessed using the Cochrane manual, and a meta-analysis of qualified literature was performed using RevMan 5.3 software. 【Results】 Seven articles were finally included, involving 421 patients with acute achilles tendon injury, including 212 patients in the PRP treatment group, and 209 patients in the conventional treatment group. The results of meta-analysis showed that there was no difference between the conventional treatment group and the PRP treatment group in terms of the visual analogue pain scale(SMD=-0.44, 95%CI: -0.94~0.06, P>0.05), calf circumference (MD=1.14, 95% CI: -1.56-3.84, P>0.05), ankle joint toe flexion range of motion (SMD=1.85, 95%CI: -1.38-5.09, P>0.05), ankle dorsiflexion range of motion(SMD=2.61, 95%CI: -0.95-6.17, P>0.05), achilles tendon fracture score (MD=-5.60, 95%CI: -15.36-4.16, P>0.05) and the maximum heel rise height (MD=-2.48, 95%CI: -5.30-0.33, P>0.05). And there was no difference in the incidence of adverse reactions between the two groups (X²=2. 455, P>0.05). 【Conclusion】 PRP injection for acute achilles tendon injury does not improve the biomechanical and clinical outcomes of patients, and the use of PRP does not increase the occurrence of adverse reactions.

Objective: To observe the prevention effect of Tui-pushing the Large Intestine Meridian plus point sticking at Tianshu (ST 25) for post-stroke constipation. Methods:Seventy eligible patients with post-stroke constipation were randomized into a treatment group and a control group, 35 cases in each group. Both groups were intervened by conventional treatment and care in Western medicine, while the treatment group was also by Tui-pushing the Large Intestine Meridian and point sticking at Tianshu (ST 25). The treatment lasted for 14 d. Therapeutic efficacy was evaluated by the first defecation time, constipation incidence and Chinese stroke scale (CSS) score. Results:After treatment, the first defecation time was (1.86±0.74) d in the treatment group, shorter than (2.77±0.83) d in the control group; constipation incidence was 14.3% in the treatment group, significantly lower than 37.1% in the control group, and the between-group differences in the two items were statistically significant (bothP<0.05). CSS score in both groups dropped significantly after treatment (bothP<0.05); the improvement in the treatment group was better than that in the control group, and the inter-group difference was statistically significant (P<0.05). Conclusion: Tui-pushing the Large Intestine Meridian plus point sticking at Tianshu (ST 25) can effectively prevent post-stroke constipation, and can also promote the rehabilitation of nerve functions.

Objective:To study the influence of Rhizoma Corydalis before and after vinegar processing on the microelement content in the eye tissues of rats to verify the vinegar processing leading the herb to liver meridian. Methods: The rats were divided into the crude Corydalis group, vinegar processing group and negative group. The content of microelement Cu, Zn and Mn in rat ocular tissues was determined and compared after the administration. Results:Corydalis could significantly increase the content of Cu and Zn in fun-dus blood and that of Zn and Mn in the eyeball vitreous, and Rhizoma Corydalis after vinegar processing showed more obvious effect. Conclusion:Rhizoma Corydalis with vinegar processing can improve the distribution of microelements in eye tissues, and because liver opens at eyes, the results provide evidence for Corydalis after vinegar processing leading to liver meridian.

Objective To investigate the species and distribution of marine bacteria off Zhoushan Archipelago of China and to analyze their antimicrobial sensitivity in this sea region.Methods Seawater samples were collected by sterile sampling devices,and then the collected bacteria were enriched,cultured,isolated and single colony was chosen for study.Bacteria identification and drug sensitivity tests were performed by VITEK-AMS,the API microbial identification system and the BIMIC Vision system.Colony counting of the seawater was also performed accordingly.Results Fifty-seven strains were isolated from 46 seawater samples,of which 28 were vibrios (49.1％),19 were enterobacteria (33.3％),8 nonfermenters (14.0％),1 Enterococcus feacium (1.8％) and 1 fungus (1.8％).Drug sensitivity tests indicated that in the more than 20 kinds of antibiotics vibrios,nonfermenters and enterobacteria were generally highly susceptible to Imipenem,Meropenem,Cefepime,Ceftazidime and Levofloxacin (＞ 95％),they could be used as first-choice antibiotics.Rates of susceptibility to Chloramphenicol,Cefotaxime,Tobramycin,Ciprofloxacin,Aztreonam,Ceftriaxone,Cefoperazone,Piperacillin and Tazobactam were within ranges of 80％-94％.They could be used as optional antibiotics.However,the marine bacteria were less susceptible to SMZ,Piperacillin,Cefuroxime,and Ampicillin (＜ 60％),which should be prohibited or used with care.Conclusions This study has helped to establish a basic database of bacterial spectrum and sensitivity of Zhoushan Archipelago.It will provide basic theoretical evidence for early prevention/treatment and selection of antibiotics in those offshore mariners who are accidentally injured and complicated with seawater immersion.

Objective To investigate the species and distribution of marine bacteria off Zhoushan Archipelago of China and to analyze their antimicrobial sensitivity in this sea region.Methods Seawater samples were collected by sterile sampling devices,and then the collected bacteria were enriched,cultured,isolated and single colony was chosen for study.Bacteria identification and drug sensitivity tests were performed by VITEK-AMS,the API microbial identification system and the BIMIC Vision system.Colony counting of the seawater was also performed accordingly.Results Fifty-seven strains were isolated from 46 seawater samples,of which 28 were vibrios (49.1％),19 were enterobacteria (33.3％),8 nonfermenters (14.0％),1 Enterococcus feacium (1.8％) and 1 fungus (1.8％).Drug sensitivity tests indicated that in the more than 20 kinds of antibiotics vibrios,nonfermenters and enterobacteria were generally highly susceptible to Imipenem,Meropenem,Cefepime,Ceftazidime and Levofloxacin (＞ 95％),they could be used as first-choice antibiotics.Rates of susceptibility to Chloramphenicol,Cefotaxime,Tobramycin,Ciprofloxacin,Aztreonam,Ceftriaxone,Cefoperazone,Piperacillin and Tazobactam were within ranges of 80％-94％.They could be used as optional antibiotics.However,the marine bacteria were less susceptible to SMZ,Piperacillin,Cefuroxime,and Ampicillin (＜ 60％),which should be prohibited or used with care.Conclusions This study has helped to establish a basic database of bacterial spectrum and sensitivity of Zhoushan Archipelago.It will provide basic theoretical evidence for early prevention/treatment and selection of antibiotics in those offshore mariners who are accidentally injured and complicated with seawater immersion.