Pulmonary hypertension(PH)is a fatal disease characterized by pulmonary vascular remodeling,ultimately leading to right heart failure and death.Current treatments for PH are suboptimal,with no substantial improvement in overall survival among patients with advanced PH.Despite some progress in understanding the pathogenesis of PH,further studies at the molecular level are needed to develop more effective treatments for PH.Recent research has shown that long non-coding RNAs(lncRNAs)have an important regulatory function in the pathophysiological process of PH,and may thus be potential disease biomarkers and therapeutic targets.In this paper,we review recent progress in our understanding of the molecular mechanisms of lncRNAs in PH.

Pulmonary hypertension (PH) is a group of progressive diseases characterized by pulmonary vascular remodeling,and some patients already have right heart insufficiency at the time of diagnosis. Therefore,early diagnosis of PH is essential to improve patients' quality of life and prolong survival. Biomarkers are an important indicator for early diagnosis of the disease,and there are many traditional biomarkers for PH diagnosis,but the sensitivity and specificity are low. With the progress of research,some new biomarkers have been shown to predict disease progression in early PH and play an important role in the early diagnosis of PH. This study reviews the research progress of biomarkers of PH from the aspects of right heart insufficiency,endothelial dysfunction,pulmonary artery smooth muscle dysfunction,inflammation,and in situ thrombosis to provide exploration direction and reference value for early diagnosis of PH.

Pulmonary hypertension(PH)is a fatal disease characterized by pulmonary vascular remodeling,ultimately leading to right heart failure and death.Current treatments for PH are suboptimal,with no substantial improvement in overall survival among patients with advanced PH.Despite some progress in understanding the pathogenesis of PH,further studies at the molecular level are needed to develop more effective treatments for PH.Recent research has shown that long non-coding RNAs(lncRNAs)have an important regulatory function in the pathophysiological process of PH,and may thus be potential disease biomarkers and therapeutic targets.In this paper,we review recent progress in our understanding of the molecular mechanisms of lncRNAs in PH.

Pulmonary hypertension (PH) is a group of progressive diseases characterized by pulmonary vascular remodeling,and some patients already have right heart insufficiency at the time of diagnosis. Therefore,early diagnosis of PH is essential to improve patients' quality of life and prolong survival. Biomarkers are an important indicator for early diagnosis of the disease,and there are many traditional biomarkers for PH diagnosis,but the sensitivity and specificity are low. With the progress of research,some new biomarkers have been shown to predict disease progression in early PH and play an important role in the early diagnosis of PH. This study reviews the research progress of biomarkers of PH from the aspects of right heart insufficiency,endothelial dysfunction,pulmonary artery smooth muscle dysfunction,inflammation,and in situ thrombosis to provide exploration direction and reference value for early diagnosis of PH.

Objective To evaluate the performance of MRI in predicting pathological response of different breast cancer subtypes after neoadjuvant chemotherapy(NAC).Methods The MRI images and postoperative pathological results of 91 patients with breast cancer after NAC were analyzed retrospectively.The correlation between the imaging features of different molecular subtypes of breast cancer and postoperative pathological results was studied,and the diagnostic performance of MRI in predicting pathological response after NAC was evaluated,with postoperative pathological results referred as the diagnostic standard.Results Of 91 patients,27(29.7％)and 35(38.5％)cases were diagnosed as imaging complete response(iCR)and pathological complete response(pCR),respectively.The accuracy of MRI in predicting pathological response after NAC was 84.62％,with 94.64％sensitivity,68.57％specificity,and positive predictive value(PPV)and negative predictive value(NPV)of 82.81％and 88.89％,respectively.Conclusion MRI can accurately predict the pathological response of the human epidermal growth factor receptor-2(HER-2)+and triple-negative breast cancer after NAC.

Objective:To explore the current situation of nursing human caring in hospital wards and analyze its influencing factors, so as to facilitate the development of nursing human caring practice.Methods:From July to November 2022, a total of 107 hospitals were surveyed through stratified convenience sampling method, and 4 072 ward nursing managers were recruited to finish the general information questionnaire and the ward nursing human caring status questionnaire. The general information included the region, class and type of the hospital, etc. The ward nursing human caring status questionnaire included 38 items in 5 dimensions of nursing human caring system and process, humanistic quality and training of nursing staff, humanistic environment and facilities, human caring procedures and measures, and human caring quality evaluation and improvement, with a full score of 190 points. Descriptive statistics were used to analyze the general data, independent samples t-test, ANOVA and correlation analysis were used to analyze the factors influencing the current status of nursing human caring in the ward, while multiple linear regression analysis was used to conduct a multivariate analysis. Results:The score of nursing human caring in hospital wards was 156.91±27.78. Whether the hospital had carried out nursing human caring pilot(demonstration) wards, whether the ward had previously been a hospital nursing human caring pilot(demonstration) nursing unit, the type of ward, and whether nursing managers had participated in human caring training were the influencing factors of the implication of nursing humanistic caring in wards( P<0.05). Conclusions:The practice of nursing human caring in hospital wards is at a good level, but needs to be further strengthened. Nursing managers should take systematically strategies to promote the development of nursing human caring practice.

Sympathetic cues via the adrenergic signaling critically regulate bone homeostasis and contribute to neurostress-induced bone loss, but the mechanisms and therapeutics remain incompletely elucidated. Here, we reveal an osteoclastogenesis-centered functionally important osteopenic pathogenesis under sympatho-adrenergic activation with characterized microRNA response and efficient therapeutics. We discovered that osteoclastic miR-21 was tightly regulated by sympatho-adrenergic cues downstream the β2-adrenergic receptor (β₂AR) signaling, critically modulated osteoclastogenesis in vivo by inhibiting programmed cell death 4 (Pdcd4), and mediated detrimental effects of both isoproterenol (ISO) and chronic variable stress (CVS) on bone. Intriguingly, without affecting osteoblastic bone formation, bone protection against ISO and CVS was sufficiently achieved by a (D-Asp₈)-lipid nanoparticle-mediated targeted inhibition of osteoclastic miR-21 or by clinically relevant drugs to suppress osteoclastogenesis. Collectively, these results unravel a previously underdetermined molecular and functional paradigm that osteoclastogenesis crucially contributes to sympatho-adrenergic regulation of bone and establish multiple targeted therapeutic strategies to counteract osteopenias under stresses.
Adrenergic Agents/pharmacology* ; Apoptosis Regulatory Proteins/pharmacology* ; Bone Diseases, Metabolic/metabolism* ; Humans ; Liposomes ; MicroRNAs/genetics* ; Nanoparticles ; Osteoclasts ; Osteogenesis/physiology* ; RNA-Binding Proteins/pharmacology*

A total of 180 type 2 diabetic patients with negative anti-thyroid autoantibodies were recruited.According to the levels of serum 25-OH vitamin D [25 (OH) D],these patients were divided into four groups:sufficient group (≥ 30 ng/ml),insufficient group (20-＜30 ng/ml),deficiency group (10-＜20 ng/ml),and severe deficiency group(＜ 10 ng/ml).The results showed that compared with sufficient vitamin D group,the group with severe deficient vitamin D had higher TT3,FT3,FT3/FT4,and TSH (all P＜0.05).The serum 25(OH) D level was negatively correlated with TSH values (r =-0.207,P＜0.05) after adjustment for gender,age,and body mass index.Multivariate regression analysis indicated that serum 25 (OH) D level was an independent influencing factor for TSH value (β =-0.201,P＜0.05),suggesting that serum 25 (OH) D level seems to be correlated with thyroid function in type 2 diabetic patients.