Objective:To explore the expression of T-cell immunoglobulin and mucin domain-containing molecule 3 (Tim-3), mitotic arrest deficient 2-like protein 1 (MAD2L1), and microRNA-203a-3p (miR-203a-3p) in colorectal cancer tissues and their relationship with prognosis.Methods:The clinicopathological data of 113 patients with colorectal cancer who underwent radical surgery in Zhengzhou Central Hospital Affiliated to Zhengzhou University from Jan. 2021 to Dec. 2021 were retrospectively analyzed. Both cancer tissues and adjacent tissues were collected postoperatively for the detection of Tim-3, MAD2L1, and miR-203a-3p. Patients were followed up for 3 years, and their prognosis was recorded and divided into a survival group (84 cases) and a death group (29 cases). Univariate and multivariate Cox regression analyses were performed to investigate the factors influencing the prognosis of colorectal cancer patients. Kaplan-Meier survival curves were used to analyze the prognostic significance of Tim-3, MAD2L1, and miR-203a-3p expression.Results:The high expression rates of Tim-3 and MAD2L1 in colorectal cancer group were higher than those in adjacent tissues ( P < 0.05), while the expression of miR-203a-3p was lower than that in adjacent tissues ( P < 0.05). Kaplan-Meier survival curve showed that the 3-year survival rate of patients with high expression of Tim-3 and MAD2L1 was lower than that of patients with low expression ( P < 0.05), while the 3-year survival rate of patients with high expression of miR-203a-3p was higher than that of patients with low expression of miR-203a-3p ( P < 0.05). Cox regression analysis showed that TNM stage III-IV, low differentiation of tissue grade, lymph node metastasis, high expression of Tim-3 and MAD2L1, and low expression of miR-203a-3p were risk factors for poor prognosis in patients with colorectal cancer ( P < 0.05) . Conclusions:Tim-3 and MAD2L1 are highly expressed in colorectal cancer tissues, while miR-203a-3p is lowly expressed. The above indicators are closely related to the poor prognosis of patients, and are expected to become potential biomarkers for prognosis evaluation of colorectal cancer patients.

Objective:To explore the expression of T-cell immunoglobulin and mucin domain-containing molecule 3 (Tim-3), mitotic arrest deficient 2-like protein 1 (MAD2L1), and microRNA-203a-3p (miR-203a-3p) in colorectal cancer tissues and their relationship with prognosis.Methods:The clinicopathological data of 113 patients with colorectal cancer who underwent radical surgery in Zhengzhou Central Hospital Affiliated to Zhengzhou University from Jan. 2021 to Dec. 2021 were retrospectively analyzed. Both cancer tissues and adjacent tissues were collected postoperatively for the detection of Tim-3, MAD2L1, and miR-203a-3p. Patients were followed up for 3 years, and their prognosis was recorded and divided into a survival group (84 cases) and a death group (29 cases). Univariate and multivariate Cox regression analyses were performed to investigate the factors influencing the prognosis of colorectal cancer patients. Kaplan-Meier survival curves were used to analyze the prognostic significance of Tim-3, MAD2L1, and miR-203a-3p expression.Results:The high expression rates of Tim-3 and MAD2L1 in colorectal cancer group were higher than those in adjacent tissues ( P < 0.05), while the expression of miR-203a-3p was lower than that in adjacent tissues ( P < 0.05). Kaplan-Meier survival curve showed that the 3-year survival rate of patients with high expression of Tim-3 and MAD2L1 was lower than that of patients with low expression ( P < 0.05), while the 3-year survival rate of patients with high expression of miR-203a-3p was higher than that of patients with low expression of miR-203a-3p ( P < 0.05). Cox regression analysis showed that TNM stage III-IV, low differentiation of tissue grade, lymph node metastasis, high expression of Tim-3 and MAD2L1, and low expression of miR-203a-3p were risk factors for poor prognosis in patients with colorectal cancer ( P < 0.05) . Conclusions:Tim-3 and MAD2L1 are highly expressed in colorectal cancer tissues, while miR-203a-3p is lowly expressed. The above indicators are closely related to the poor prognosis of patients, and are expected to become potential biomarkers for prognosis evaluation of colorectal cancer patients.