Objective TostudytheeffectofmicroRNA(miRNA) on the expression ofVEGF in ovarian cancer cells and its effect on tumor cell proliferation,in order to provide a new way for clinical diagnosis and treatment.Methods The two transfected cells were divided into HO-8910-H1-VEGF group and HO-8910-H1 group,and untransfected cells were referred to as the control group(HO-8910 group) by MTr(tetrazolium salt) method.The effect of miRNA on the proliferation of ovarian cancer cells was observed by periodic analysis.Results The expression of VEGF in HO-8910 cells was significantly decreased after transfection with specific small hairpin miRNA plasmid expression vector,ie,the expression of VEGF in HO-8910 H1-VEGF group was decreased compared with HO-8910 H1 group and HO-8910 group;Indirect immunofluorescence assay showed that the HO-8910-H1-VEGF group showed weak fluorescence intensity,but the HO-8910 H1 group and HO-8910 group had stronger fluorescence intensity.Cells in the HO-8910-H1-VEGF group that were blocked in the phase G1 (inter val phase 1) increased by 24％,and cells in phase S (DNA synthesis phase) showed a 21％ reduction.Conclusion MicroRNA can significantly inhibit the expression of VEGF in ovarian cancer HO-8910 cells,thereby blocking the proliferation of ovarian cancer cells and providing a theoretical basis for clinical gene therapy.

Objective To investigate the clinical effect of enzyme linked immunosorbent assay (ELISA) in detecting hepatitis C antibody. Methods 200 cases of patients with hepatitis C was measured by ELISA, the gold standard method for detection of hepatitis C virus antibody, recording the test results, the data input SPSS software to give the corresponding analysis and conclusions. Results 200 cases of patients with hepatitis C were successfully completed the gold standard method and ELISA method for detection of hepatitis C virus antibody, the analysis showed that positive rate of ELISA method for detection of hepatitis C virus antibody was significantly higher than that of the gold standard method, the positive rate of ELISA was 82.50％, the positive rate of the gold standard method for was 50.00％, results suggested that HCV detection rate of ELISA method is significantly better (P<0.05). Conclusion The method of ELISA can be used for the detection of hepatitis C antibody, which has positive significance in improving the diagnostic accuracy of hepatitis C, and is beneficial to protect the efficacy and quality of life of patients.