Objective To investigate the expression of X-linked inhibitor of apoptosis associated factor 1(XAF1)and phosphoenolpyruvate carboxykinase 1(PCK1)in Luminal type B breast cancer and their relationship with the prognosis of patients.Methods From January 2016 to January 2018,95 patients with Luminal B breast cancer underwent surgical treatment.Cancer tissues and adjacent tissues＞5 cm from the cancer tissues were collected during the operation.The positive expressions of XAF1 and PCK1 proteins were detected by immunohistochemistry.The relationship between XAF1 and PCK1 protein expression and clinicopathological features of breast cancer was analyzed,and the survival curves of breast cancer patients with different XAF1 and PCK1 protein expression were plotted by Kaplan-Meier method,the survival rate was compared by Log-rank test.Multi-factor Cox-regression analysis was used to analyze the prognostic factors of breast cancer.Results GEPIA database analysis showed that the expression levels of XAF1 and PCK1 mRNA in breast cancer tissues were lower than those in normal tissues(P＜0.05).The positive expression rates of XAF1 and PCK1 proteins in cancer tissue were lower than adjacent tissues(P＜0.05).The positive expression of XAF1 in breast cancer tissue was related to clinical stage,lymph node metastasis and histological grade,Ki-67 expression status,while the positive expression of PCK1 was related to clinical stage,lymph node metastasis and Ki-67 expression status(P＜0.05).The 5-year overall survival rate of XAF1 positive expression patients was higher than that of negative expression patients(Log-rank x2=5.101,P=0.024),and the 5-year overall survival rate of PCK1 positive expression patients was higher than that of negative expression patients(Log-rank x2=6.515,P=0.011).Clinical stage Ⅲ,negative expression of XAF1,and negative expression of PCK1 were risk factors for poor prognosis(P＜0.05).Conclusion The positive expression rates of XAF1 and PCK1 in Luminal B type breast cancer tissue are reduced,which is related to some clinicopathological characteristics and 5-year survival rate after surgery.The 5-year survival rate was lower in patients with negative XAFI and PCKI expression.

Objective To investigate the expression of X-linked inhibitor of apoptosis associated factor 1(XAF1)and phosphoenolpyruvate carboxykinase 1(PCK1)in Luminal type B breast cancer and their relationship with the prognosis of patients.Methods From January 2016 to January 2018,95 patients with Luminal B breast cancer underwent surgical treatment.Cancer tissues and adjacent tissues＞5 cm from the cancer tissues were collected during the operation.The positive expressions of XAF1 and PCK1 proteins were detected by immunohistochemistry.The relationship between XAF1 and PCK1 protein expression and clinicopathological features of breast cancer was analyzed,and the survival curves of breast cancer patients with different XAF1 and PCK1 protein expression were plotted by Kaplan-Meier method,the survival rate was compared by Log-rank test.Multi-factor Cox-regression analysis was used to analyze the prognostic factors of breast cancer.Results GEPIA database analysis showed that the expression levels of XAF1 and PCK1 mRNA in breast cancer tissues were lower than those in normal tissues(P＜0.05).The positive expression rates of XAF1 and PCK1 proteins in cancer tissue were lower than adjacent tissues(P＜0.05).The positive expression of XAF1 in breast cancer tissue was related to clinical stage,lymph node metastasis and histological grade,Ki-67 expression status,while the positive expression of PCK1 was related to clinical stage,lymph node metastasis and Ki-67 expression status(P＜0.05).The 5-year overall survival rate of XAF1 positive expression patients was higher than that of negative expression patients(Log-rank x2=5.101,P=0.024),and the 5-year overall survival rate of PCK1 positive expression patients was higher than that of negative expression patients(Log-rank x2=6.515,P=0.011).Clinical stage Ⅲ,negative expression of XAF1,and negative expression of PCK1 were risk factors for poor prognosis(P＜0.05).Conclusion The positive expression rates of XAF1 and PCK1 in Luminal B type breast cancer tissue are reduced,which is related to some clinicopathological characteristics and 5-year survival rate after surgery.The 5-year survival rate was lower in patients with negative XAFI and PCKI expression.

Objective To investigate the expression of nuclear receptor interacting protein 1(NRIP1)and dual specificity phosphatase(DUSP14)in breast cancer tissues and their correlation with the prognosis of breast cancer patients.Methods A total of 124 patients with breast cancer admitted to this hospital from June 2015 to January 2018 were selected as the research objects,and were followed up for 60 months after surgery.Immunohistochemistry was used to detect the expression of NRIP1 and DUSP14 in breast cancer tissues and adjacent normal tissues.The relationship between NRIP1 and DUSP14 expression and clinicopathological fea-tures of breast cancer was analyzed.Multivariate Cox regression was used to analyze the prognostic factors of breast cancer patients.Kaplan-Meier survival curve was used to analyze the 5-year survival rate of breast canc-er patients.Results Compared with the adjacent tissues,the breast cancer tissues had a significantly higher positive expression rate of NRIP1 and a significantly lower positive expression rate of DUSP14(P＜0.05).The expression level of NRIP1 in breast cancer tissues was related to histological grade,clinical stage,lymph node metastasis,estrogen receptor and Ki-67(P＜0.05).The expression level of DUSP14 was correlated with histological grade,clinical stage,lymph node metastasis and Ki-67 expression(P＜0.05).The 5-year survival rate of patients with negative NRIP1 expression was significantly higher than that of patients with positive NRIP1 expression(P＜0.05),and the 5-year survival rate of patients with negative DUSP14 expression was significantly lower than that of patients with positive DUSP14 expression(P＜0.05).NRIP1,clinical stage,and lymph node metastasis were risk factors for poor prognosis of breast cancer patients(P＜0.05),and DUSP14 was a protective factor(P＜0.05).Conclusion High expression of NRIP1 and low expression of DUSP14 are associated with poor prognosis in breast cancer.