Objective:To explore the short-term outcomes of 3D-printing stand-alone artificial vertebral body(AVB)in the surgical procedure of anterior cervical corpectomy and fusion(ACCF).Methods:Following the proposal of IDEAL(idea,development,exploration,assessment,and long-term follow-up)framework,we designed and conducted this single-armed,retrospective cohort study.The patients with cervical spondylotic myelopathy were recruited,and these patients exclusively received the surgical procedure of single-level ACCF in our single center.After the process of corpectomy,the size was tailored using different trials and the most suitable stand-alone AVB was then implanted.This AVB was manufactured by the fashion of 3D-printing.Two pairs of screws were inserted in an inclined way into the adjacent vertebral bodies,to stabilize the AVB.The participants were regularly followed-up after the operation.Their clinical data were thoroughly reviewed.We assessed the neurological status according to Japanese Orthopedic Association(JOA)scale.We determined the fusion based on imaging examination six months after the operation.The recorded clinical data were analyzed using specific software and they presented in suitable styles.Paired t test was employed in comparison analysis.Results:In total,there were eleven patients being recruited eventually.The patients were all followed up over six months after the operation.The mean age of the cohort was(57.2±10.2)years.The mean operation time was(76.1±23.1)min and the median bleeding volume was 150(100,200)mL.The postoperative course was uneventful for all the cases.Dysphagia,emergent hematoma,and deterioration of neurological func-tion did not occur.Mean JOA scores were 13.2±2.2 before the operation and 16.3±0.8 at the final follow-up,which were significantly different(P＜0.001).The mean recovery rate of neurological func-tion was 85.9％.By comparing the imaging examinations postoperatively and six months after the opera-tion,we found that the average subsidence length was(1.2±1.1)mm,and that there was only one ca-ses(9.1％)of the severe subsidence(＞3 mm).We observed significant improvement of cervical lor-dosis after the operation(P=0.013).All the cases obtained solid fusion.Conclusion:3D-printing stand-alone AVB presented favorable short-term outcome in one-level ACCF in this study.The fusion rate of this zero-profile prosthesis was satisfactory and the complication rate was relatively low.

Objective:To explore the clinical efficacy of chemoembolization with doxorubicin-infused gelatin sponge microparticles in the amelioration of arterioportal shunting (APS) in patients afflicted with hepatocellular carcinoma (HCC).Methods:A retrospective investigation was conducted on 9 HCC patients admitted between January 2020 and December 2022 with concomitant moderate-to-severe APS who underwent GSM-transarterial chemoembolization (TACE). Hepatic artery digital subtraction angiography (DSA) was employed to ascertain the magnitude of improvement in arteriovenous shunts, utilization of modified response evaluation criteria in solid tumors facilitated the appraisal of short-term clinical outcomes. Follow-up records documented survival duration, along with quantitative parameters such as the longest diameter of tumor lesions and serum alpha-fetoprotein (AFP) levels before and after treatment. The Wilcoxon rank-sum test was utilized to compare the differences of these quantitative parameters before and after treatment.Results:The APS amelioration rates were 100% and 88.9% at immediate and recent postoperatively, respectively. The oncological response rate was 77.8% (7/9), and the complete necrosis rate was 22.2% (2/9) at three months postoperatively, the 1-year survival rate was 100%. Following treatment, a significant reduction was observed in the tumor′s longitudinal diameter [4.32(3.88,6.63)cm] and serum AFP levels [13.50 (7.55, 29.60) μg/L], compared to the pre-treatment values of the tumor′s longitudinal diameter [5.20(4.58,8.57)cm] and serum AFP levels [524.30 (320.65, 1 046.15) μg/L] ( P<0.05 for all). Conclusion:Doxorubicin-infused GSM-TACE is both feasible and efficacious in the first treatment of HCC concomitant with APS and represents a better clinical alternative.

OBJECTIVE: To explore the interaction between Tubulin beta 4B class IVb (TUBB4B) and Agtpbp1/cytosolic carboxypeptidase- like1 (CCP1) in mouse primary spermatocytes (GC-2 cells) and the role of TUBB4B in regulating the development of GC-2 cells. METHODS: Lentiviral vectors were used to infect GC-2 cells to construct TUBB4B knockdown and negative control (NC-KD) cells. The stable cell lines with TUBB4B overexpression (Tubb4b-OE) and the negative control (NC-OE) cells were screened using purinomycin. RT-qPCR and Western blotting were used to verify successful cell modeling and explore the relationship between TUBB4B and CCP1 expressions in GC-2 cells. The effects of TUBB4B silencing and overexpression on the proliferation and cell cycle of GC-2 cells were evaluated using CCK8 assay and flow cytometry. The signaling pathway proteins showing significant changes in response to TUBB4B silencing or overexpression were identified using Western blotting and immunofluorescence assay and then labeled for verification at the cellular level. RESULTS: Both TUBB4B silencing and overexpression in GC-2 cells caused consistent changes in the mRNA and protein expressions of CCP1 (P < 0.05). Similarly, TUBB4B expression also showed consistent changes at the mRNA and protein after CCP1 knockdown and restoration (P < 0.05). TUBB4B knockdown and overexpression had no significant effect on proliferation rate or cell cycle of GC-2 cells, but caused significant changes in the key proteins of the nuclear factor kappa-B (NF-κB) signaling pathway (p65 and p-p65) and the mitogen-activated protein kinase (MAPK) signaling pathway (ErK1/2 and p-Erk1/2) (P < 0.05); CCP1 knockdown induced significant changes in PolyE expression in GC-2 cells (P < 0.05). CONCLUSIONS TUBB4B and CCP1 interact via a mutual positive regulation mechanism in GC-2 cells. CCP-1 can deglutamize TUBB4B, and the latter is involved in the regulation of NF-κB and MAPK signaling pathways in primary spermatocytes.
Animals ; Male ; Mice ; GTP-Binding Proteins/metabolism* ; Mitogen-Activated Protein Kinases/metabolism* ; NF-kappa B/metabolism* ; RNA, Messenger ; Serine-Type D-Ala-D-Ala Carboxypeptidase/metabolism* ; Signal Transduction ; Spermatocytes ; Tubulin/genetics*

The primary cause of injury and death in the elderly has been reflected in fall the elderly, so the application of reasonable and effective prevention strategies has great significance in reducing the risk of fall in the elderly. The research progress of virtual reality technology applied in preventing fall in the elderly at home and abroad over the years was systematically reviewed in this study. The mechanism of the technology in preventing fall in the elderly was mainly elaborated from five aspects of improving balance ability, gait disturbance, cognitive impairment, muscle strength and the fear psychology of falling. The purpose of this thesis is to broaden the research ideas of medical personnel on the prevention of fall of the elderly, provide more effective clinical practice plans, reduce the occurrence of fall, and guarantee the safety of the elderly.
Aged ; Humans ; Gait ; Muscle Strength ; Technology ; Virtual Reality

Long non-coding RNAs (lncRNAs) are a group of non-coding RNAs with a length of over 200 nucleotides. Recently, studies have shown that lncRNAs can regulate gene expression at the epigenetic, transcriptional, and post-transcriptional levels, affect the proliferation, migration, and infiltration of tumor cells, and participate in the occurrence and development of benign and malignant proliferative skin diseases, such as keloids, hemangioma, squamous cell carcinoma and melanoma. This review summarizes roles of lncRNAs in the pathogenesis of benign and malignant proliferative skin diseases.

Objective:To establish and verify a nomogram model for predicting the risk of non-alcoholic fatty liver disease (NAFLD) in helicopter flying personnel.Methods:The helicopter flying personnel who recuperated at Air Force Healthcare Center for Special Services Hangzhou were selected as the study subjects. They were divided into the training group (75%) and the verification group (25%) by using the cluster random sampling method for internal verification. Multivariate Logistic regression analysis was used to analyze the factors influencing NAFLD of helicopter flying personnel, and the nomogram model was constructed by R4.2.1 software and rms package. The H-L goodness-of-fit test and receiver operating characteristic (ROC) curve were used to assess the discrimination and calibration of the prediction model, and decision curve analysis (DCA) was used to evaluate the clinical practicality of the model.Results:A total of 2 195 helicopter flying personnel were included, with 1 648 in the training group and 547 in the verification group. Seven variables, including age, BMI, hyperuricemia, hypertriglyceridemia, abnormal high-density lipoprotein, daily intakes of milk and/or its products and years of smoking were used as the predictors of the nomogram model for the risk prediction of NAFLD in helicopter flying personnel. The H-L goodness-of-fit tests of training group and verification group were χ2=8.54, 11.03, P=0.382, 0.200, respectively, indicating a good predictive accuracy. The area under the ROC curve of the 2 groups was 0.840 and 0.860, respectively, indicating a certain degree of discrimination. The incidence risk probability of DCA curve in 2 groups was about 10%-30%, and the net return rate was above 0, suggesting that it had a certain clinical applicability. Increased age ( OR=1.18, P=0.010), overweight and obesity ( OR=3.67, P<0.001), hyperuricemia ( OR=2.12, P<0.001), hypertriglyceridemia ( OR=2.96, P<0.001), abnormal high-density lipoprotein cholesterol ( OR=1.83, P=0.010), and increased years of smoking ( OR=1.20, P=0.010) were the risk factors for NAFLD in helicopter flying personnel, while increased daily intakes of milk and/or its products ( OR=0.76, P=0.020) were identified as a protective factor. Conclusions:The nomogram model based on the aforementioned factors can effectively evaluate the incidence risk of NAFLD in helicopter flying personnel, and has certain clinical practicality.

Objective:To establish and verify a nomogram model for predicting the risk of non-alcoholic fatty liver disease (NAFLD) in helicopter flying personnel.Methods:The helicopter flying personnel who recuperated at Air Force Healthcare Center for Special Services Hangzhou were selected as the study subjects. They were divided into the training group (75%) and the verification group (25%) by using the cluster random sampling method for internal verification. Multivariate Logistic regression analysis was used to analyze the factors influencing NAFLD of helicopter flying personnel, and the nomogram model was constructed by R4.2.1 software and rms package. The H-L goodness-of-fit test and receiver operating characteristic (ROC) curve were used to assess the discrimination and calibration of the prediction model, and decision curve analysis (DCA) was used to evaluate the clinical practicality of the model.Results:A total of 2 195 helicopter flying personnel were included, with 1 648 in the training group and 547 in the verification group. Seven variables, including age, BMI, hyperuricemia, hypertriglyceridemia, abnormal high-density lipoprotein, daily intakes of milk and/or its products and years of smoking were used as the predictors of the nomogram model for the risk prediction of NAFLD in helicopter flying personnel. The H-L goodness-of-fit tests of training group and verification group were χ2=8.54, 11.03, P=0.382, 0.200, respectively, indicating a good predictive accuracy. The area under the ROC curve of the 2 groups was 0.840 and 0.860, respectively, indicating a certain degree of discrimination. The incidence risk probability of DCA curve in 2 groups was about 10%-30%, and the net return rate was above 0, suggesting that it had a certain clinical applicability. Increased age ( OR=1.18, P=0.010), overweight and obesity ( OR=3.67, P<0.001), hyperuricemia ( OR=2.12, P<0.001), hypertriglyceridemia ( OR=2.96, P<0.001), abnormal high-density lipoprotein cholesterol ( OR=1.83, P=0.010), and increased years of smoking ( OR=1.20, P=0.010) were the risk factors for NAFLD in helicopter flying personnel, while increased daily intakes of milk and/or its products ( OR=0.76, P=0.020) were identified as a protective factor. Conclusions:The nomogram model based on the aforementioned factors can effectively evaluate the incidence risk of NAFLD in helicopter flying personnel, and has certain clinical practicality.

Objective:To develop the Nursing Humanistic Care Needs Questionnaire for Intensive Care Unit (ICU) Patients, and test its reliability and validity.Methods:The Nursing Humanistic Care Needs Questionnaire for ICU Patients was developed through literature review, semi-structured interview, group discussion, Delphi expert consultation and pre-investigation. From November to December 2019, purpose sampling was used to select 280 ICU patients from 3 Class Ⅲ Grade A hospitals in Changsha for investigation, and the reliability and validity of the questionnaire were tested.Results:After project analysis and 2 rounds of exploratory factor analysis, a total of 5 common factors were identified, and the cumulative variance contribution was 76.446%. The scale-level content validity index was 0.917. The Cronbach's α coefficient of the total questionnaire was 0.962, and the test-retest reliability was 0.879, and the half-reliability coefficient was 0.969. The final version of the questionnaire included 5 dimensions and 35 items.Conclusions:The Nursing Humanistic Care Needs Questionnaire for ICU Patients has good reliability and validity, and can be used to evaluate the nursing humanistic care needs of ICU patients.

Objective:To design an easy-to-use health risk assessment and management system with reasonable structures and advanced technology for divers，and to provide data and digital support for the assessment and maintenance of military operational capability of specific personnel.Methods:The process of health risk assessment and management for divers was standardized. And a set of supporting subsystems was designed and implemented，including the systems of risk assessment，health intervention，and offline collection of health data.Results:The health risk assessment and management system for divers was successfully built. The process of health risk assessment and management for divers was standardized. And the responsibilities of relevant personnel were specifically defined. Thus，a high level of health data sharing and the effective utilization of recuperation facilities were achieved.Conclusion:The application of the system has standardized the health risk management and ensured individually-tailored health support. Therefore，it has effectively helped maintain the health and the military operational capability of divers.

Objective:To design an easy-to-use health risk assessment and management system with reasonable structures and advanced technology for divers，and to provide data and digital support for the assessment and maintenance of military operational capability of specific personnel.Methods:The process of health risk assessment and management for divers was standardized. And a set of supporting subsystems was designed and implemented，including the systems of risk assessment，health intervention，and offline collection of health data.Results:The health risk assessment and management system for divers was successfully built. The process of health risk assessment and management for divers was standardized. And the responsibilities of relevant personnel were specifically defined. Thus，a high level of health data sharing and the effective utilization of recuperation facilities were achieved.Conclusion:The application of the system has standardized the health risk management and ensured individually-tailored health support. Therefore，it has effectively helped maintain the health and the military operational capability of divers.