Objective To observe the improvement of motor dysfunction and serum levels of C-reactive protein(CRP),blood glucose and blood lipid in post-stroke patients treated with scalp acupuncture at different needle retention time.Methods A total of 120 patients with motor dysfunction after stroke were randomly divided into control group,observation group 1 and observation group 2,with 40 cases in each group.The patients in the 3 groups were treated with scalp acupuncture,body acupuncture and routine rehabilitation exercise,once a day and 6 times a week,lasting for 2 weeks.The control group was given scalp acupuncture with retaining of needles for 30 minutes,the observation group 1 was given scalp acupuncture with retaining of needles for one hour,and the observation group 2 was given scalp acupuncture with retaining of needles for 2 hours.Before and after treatment,the 3 groups were observed in the changes of the scale scores of National Institutes of Health Stroke Scale(NIHSS),Fugl-Meyer Assessment(FM A),Berg Balance Scale(BBS)and modified Barthel Index(MBI),and the levels of laboratory indicators of peripheral blood CRP,fasting plasma glucose(FPG),total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C).After treatment,the clinical safety of the three groups was evaluated.Results(1)After treatment,the scale scores of NIHSS in the three groups were lower(P＜0.01)and the scale scores FMA,BBS and MBI were higher than those before treatment(P＜0.05 or P＜0.01).The comparison of post-treatment scale scores showed that the differences among the three groups were statistically significant(P＜0.01).The intergroup comparison showed that the decrease of NIHSS score and the increase of FMA,BBS and MBI scores in the observation group 2 were significantly superior to those in the control group and the observation group 1(P＜0.01);the improvement of FMA score in the observation group 1 was significantly superior to that in the control group(P＜0.01),while the improvement of NIHSS,BBS and MBI scores tended to be superior to that in the control group without statistically significant differences(P＞0.05).The results indicated that the curative effect of scalp acupuncture plus exercise regimen was positively correlated with the duration of needle retention for scalp acupuncture.(2)After treatment,the laboratory indicator levels of CRP and FPG in the peripheral blood of the three groups,the levels of TG and LDL-C in the two observation groups and the level of HDL-C in the observation group 2 were improved compared with those before treatment(P＜0.05 or P＜0.01).Statistically significant differences were presented in the post-treatment levels of CRP and TG in peripheral blood among the three groups(P＜0.05 or P＜0.01).The intergroup comparison showed that the improvement of CRP and TG levels in the observation group 2 was significantly superior to that in the control group,and the improvement of CRP level in the observation group 2 was significantly superior to that in the observation group 1,the differences being statistically significant(P＜0.05 or P＜0.01).The TC level in the three groups after treatment did not differ from that before treatment,and there was no significant difference in TC level after treatment among the three groups either(P＞0.05).(3)During the treatment,no adverse reactions such as fainting,needle breaking and hematoma occurred in the three groups,the vital signs of the patients were stable,and there were no obvious abnormal changes in pulse,blood pressure and respiratory rate.Conclusion Scalp acupuncture can effectively improve the motor function of post-stroke patients in a pasitive time-effect relationship with the needle retention,and better the curative effect can be achieved by retaining of the needle for 2 h.

Objective: To analyze the etiological and epidemiological characteristics of Vibrio cholerae in Beijing during 2015-2021 and provide evidence for the prevention and control of cholera. Methods: The V. cholerae strains isolated in Beijing during 2015-2021 were analyzed by serotyping and virulence genes detection. Pulsed field gel electrophoresis (PFGE) was performed for the molecular typing of the strains. Based on the collected epidemiological and clinical data of cholera cases,the epidemiological characteristics of cholera were analyzed by descriptive epidemiology method. Results: A total of 76 Vibrio cholerae O1 strains were isolated in Beijing during 2015-2021, including 61 strains from human, 10 strains from environment and 5 strains from seafood. The 76 strains consisted of 68 Ogawa strains and 8 Inaba strains. Six Ogawa strains isolated from sporadic cases carried ctxAB. After NotⅠ digestion, 76 strains were divided into 33 PFGE patterns. From 2015 to 2021, a total of 38 cholera epidemics were reported in Beijing, most of them were sporadic ones, accounting for 92.11% (35/38). A total of 45 cases were reported, and the cases occurred during June-September accounted for 97.78% (44/45). Cholera cases occurred in 9 districts of Beijing, and the cases reported in Chaoyang district accounted for 42.22% (19/45) and in Changping district accounted for 31.11% (14/45). The age of the cholera cases ranged from 19 to 63 years. Except for one case with unknown clinical symptoms, 44 cases had diarrhea symptoms with 84.09% (37/44) of the cases reporting diarrhea (3-9 times/day), followed by yellow watery stool (95.45%, 42/44), abdominal pain (68.18%, 30/44), nausea and vomiting (40.91%, 18/44) and fever (36.36%, 16/44). Conclusion: Vibrio cholerae strains isolated in Beijing during 2015-2021 were mainly O1 serotype Ogawa,most of which were non-toxigenic. The PFGE of the strains varied. Cholera epidemics occurred in 9 districts of Beijing, but most were sporadic ones with incidence peak during June-September.
Adult ; Beijing/epidemiology* ; Cholera/epidemiology* ; Diarrhea/epidemiology* ; Electrophoresis, Gel, Pulsed-Field ; Humans ; Middle Aged ; Vibrio cholerae O1/genetics* ; Young Adult

OBJECTIVETo investigate antimicrobial resistance of Klebsiella pneumoniae and Pseudomonas aeruginosa isolates in fecal samples from rat-like animals.

METHODSRat-like animals were captured using cages around a hospital and the neighboring residential area between March and October, 2015. K. pneumoniae and P. aeruginosa were isolated from the fecal samples of the captured animals. Antimicrobial susceptibility test was performed according to the guidelines of Clinical and Laboratory Standards Institute (2014).

RESULTSA total of 329 rat-like animals were captured, including 205 Suncus murinus, 111 Rattus norvegicus, 5 Rattus flavipectus and 8 Mus musculus. The positivity rates of K. pneumoniae and P. aeruginosa were 78.4% and 34.7% in the fecal samples from the captured animals, respectively. K. pneumoniae isolates from Suncus murinus showed a high resistance to ampicillin, cephazolin, nitrofurantoin, piperacillin and cefotaxime (with resistance rates of 100%, 51.2%, 44.2%, 37.2%, and 23.3%, respectively), and K. pneumoniae isolates from Rattus spp. showed a similar drug-resistance profile. The prevalence rates of multidrug resistance and ESBLs were 40.9% and 10.7%, respectively. P. aeruginosa from both Suncus murinus and Rattus spp. exhibited the highest resistance rates to aztreonam (12.4% and 16.0%, respectively), followed by penicillins and fluoroquinolones. P. aeruginosa isolates were susceptible to cephems, aminoglycosides and carbapenems (with resistance rates below 5%).

CONCLUSIONK. pneumoniae and P. aeruginosa isolated from rat-like animals showed drug-resistance profiles similar to those of the strains isolated from clinical patients, suggesting that the possible transmission of K. pneumoniae and P. aeruginosa between rat-like animals and human beings.

Animals ; Anti-Bacterial Agents ; pharmacology ; Carbapenems ; pharmacology ; Drug Resistance, Bacterial ; Fluoroquinolones ; pharmacology ; Humans ; Klebsiella pneumoniae ; drug effects ; isolation & purification ; Mice ; Murinae ; microbiology ; Pseudomonas aeruginosa ; drug effects ; isolation & purification ; Rats

Objective To investigate the mechanism involving the apoptosis of BGC-823 strain induced by miRNA-143. Methods Fluorometry and Western blot were used to detect Bcl-2 mRNA and the protein contents in the gastric cancer strain ( BGC-823)and normal gastric mucosa strain (GES-1).We verified the binding site by a Lnciferase reporter method .MiRNA-143-mimics, in-hibitor and NC were chemically synthesized .Bcl-2 protein levels were measured by Western blotting 48 hours after transiently transfect-ed with either miRNA-143 mimics, inhibitors, or negative control miRNAs into BGC-823 cells.Apoptosis assays were employed to eval-uate cell apoptosis in BGC-823 strain, and miRNA-143 expression levels in BGC-823 were measured by quantitative PCR ( qPCR) sim-ultaneously at different time points after transfection .Results The study successfully predicted and verified the putative miRNA-143 binding site in the 3′UTR of human Bcl-2 gene.Bcl-2 level in BGC-823 cells was significantly increased and miRNA-143 level was de-creased, as compared with that of GES-1 cells, and significant differences could be noted , when comparisons were made between the groups(P<0.01).Research results indicated that miRNA-143-mimics/miRNA-143-inhibitor and the wild type luciferase reporter gene co-transfection significantly reduced and induced the activity of luciferase .Forty-eight hours after transfection , statistical significance could be noticed, when the co-transfection group was compared with the single transfection group (P<0.05).The transfection of miR-NA-143-mimics and miRNA-143-inhibitors had no significant effects on the activity of mutant luciferase reporter gene .Statistical signifi-cance could be seen, when the co-transfection group was compared with the single transfection group (P<0.05).Furthermore, the transfection of miRNA-143-mimics and miRNA-143-inhibitor could significantly inhibit or enhance the expression of Bcl-2 protein, and statistical significance could be found , when it was compared with the control group (P<0.05).miRNA-143-mimics and miRNA-143-inhibitor transfected cells could induce changes in miRNA-143 level.Forty-eight hours after transfection , miRNA-143 level in the miR-NA-143-mimics transfected cells was obviously elevated , while that in the miRNA-143-inhibitor was significantly reduced , and statisti-cal significance could also be noted , when it was compared with the control group (P<0.05).There were no significant differences in miRNA-143 levels, when the negative control group was compared with the transfected control group (P<0.05).Results of apoptosis detection indicated that the elevated expression level of miRNA-143 could significantly induce apoptosis of BGC-823, and statistical sig-nificance could be seen, when the gene intervention group was compared with the transfected control group (P<0.05).Conclusion Through the inhibition of Bcl-2 gene expression, miRNA-143 could induce the apoptosis of the gastric cancer BGC-823 strain.

Objective To investigate the mechanism involving the apoptosis of BGC-823 strain induced by miRNA-143. Methods Fluorometry and Western blot were used to detect Bcl-2 mRNA and the protein contents in the gastric cancer strain ( BGC-823)and normal gastric mucosa strain (GES-1).We verified the binding site by a Lnciferase reporter method .MiRNA-143-mimics, in-hibitor and NC were chemically synthesized .Bcl-2 protein levels were measured by Western blotting 48 hours after transiently transfect-ed with either miRNA-143 mimics, inhibitors, or negative control miRNAs into BGC-823 cells.Apoptosis assays were employed to eval-uate cell apoptosis in BGC-823 strain, and miRNA-143 expression levels in BGC-823 were measured by quantitative PCR ( qPCR) sim-ultaneously at different time points after transfection .Results The study successfully predicted and verified the putative miRNA-143 binding site in the 3′UTR of human Bcl-2 gene.Bcl-2 level in BGC-823 cells was significantly increased and miRNA-143 level was de-creased, as compared with that of GES-1 cells, and significant differences could be noted , when comparisons were made between the groups(P<0.01).Research results indicated that miRNA-143-mimics/miRNA-143-inhibitor and the wild type luciferase reporter gene co-transfection significantly reduced and induced the activity of luciferase .Forty-eight hours after transfection , statistical significance could be noticed, when the co-transfection group was compared with the single transfection group (P<0.05).The transfection of miR-NA-143-mimics and miRNA-143-inhibitors had no significant effects on the activity of mutant luciferase reporter gene .Statistical signifi-cance could be seen, when the co-transfection group was compared with the single transfection group (P<0.05).Furthermore, the transfection of miRNA-143-mimics and miRNA-143-inhibitor could significantly inhibit or enhance the expression of Bcl-2 protein, and statistical significance could be found , when it was compared with the control group (P<0.05).miRNA-143-mimics and miRNA-143-inhibitor transfected cells could induce changes in miRNA-143 level.Forty-eight hours after transfection , miRNA-143 level in the miR-NA-143-mimics transfected cells was obviously elevated , while that in the miRNA-143-inhibitor was significantly reduced , and statisti-cal significance could also be noted , when it was compared with the control group (P<0.05).There were no significant differences in miRNA-143 levels, when the negative control group was compared with the transfected control group (P<0.05).Results of apoptosis detection indicated that the elevated expression level of miRNA-143 could significantly induce apoptosis of BGC-823, and statistical sig-nificance could be seen, when the gene intervention group was compared with the transfected control group (P<0.05).Conclusion Through the inhibition of Bcl-2 gene expression, miRNA-143 could induce the apoptosis of the gastric cancer BGC-823 strain.

Objective To investigate the effect of the changes in MEG3 level on proliferation activity in Lovo cells, through the amplification of the non-coding RNA-MEG3 by PCR and the construction of a eukaryotic expression vector for MEG3, which was transfect-ed into a human colon cancer cell line, Lovo.Methods cDNA was prepared from the total RNA extracted from 293 cells by reverse tran-scription and MEG3 gene was amplified by PCR and was used to construct a recombinant plasmid, which was transfected into Lovo cells by using cationic liposome.Transfection efficiency was evaluated by observation on the expression of GFP marker 48 hours after transfection, and changes in MEG3 content were detected by RT-PCR ( Real-time-PCR) .CCK-8 was used to measure the effect of genetic intervention on proliferative activity in tumor cells in the logarithmic growth phase.Results MEG3 gene was successfully obtained and the recombinant expression vector was constructed.Lovo cells were successfully transfected, and 48 hours after transfection, the transfection efficiency reached as high as 60%.MEG3 level in transfected cells was significantly increased for approximately 6.8 folds, as compared with that of the transfected control group(P<0.01).High expression of exogenous MEG3 in Lovo cells could inhibit cell proliferation, and significant differences could be noted at hours 48 and 72 after the genetic intervention, as compared with those of the untransfected group and the transfected control group(P<0.01).Conclusion LncRNA-MEG3 could obviously inhibit the proliferation of Lovo cells.

Objective To investigate the effect of the changes in MEG3 level on proliferation activity in Lovo cells, through the amplification of the non-coding RNA-MEG3 by PCR and the construction of a eukaryotic expression vector for MEG3, which was transfect-ed into a human colon cancer cell line, Lovo.Methods cDNA was prepared from the total RNA extracted from 293 cells by reverse tran-scription and MEG3 gene was amplified by PCR and was used to construct a recombinant plasmid, which was transfected into Lovo cells by using cationic liposome.Transfection efficiency was evaluated by observation on the expression of GFP marker 48 hours after transfection, and changes in MEG3 content were detected by RT-PCR ( Real-time-PCR) .CCK-8 was used to measure the effect of genetic intervention on proliferative activity in tumor cells in the logarithmic growth phase.Results MEG3 gene was successfully obtained and the recombinant expression vector was constructed.Lovo cells were successfully transfected, and 48 hours after transfection, the transfection efficiency reached as high as 60%.MEG3 level in transfected cells was significantly increased for approximately 6.8 folds, as compared with that of the transfected control group(P<0.01).High expression of exogenous MEG3 in Lovo cells could inhibit cell proliferation, and significant differences could be noted at hours 48 and 72 after the genetic intervention, as compared with those of the untransfected group and the transfected control group(P<0.01).Conclusion LncRNA-MEG3 could obviously inhibit the proliferation of Lovo cells.

OBJECTIVETo detect the expression of phosphorylated-signal transducer and activator of transcription 3 (p-Stat3) and myeloid leukemia-1 (Mcl-1) as well as their correlation, and to investigate the functional role of Stat3 and Mcl-1 in the pathogenesis of esophageal squamous cell carcinoma (ESCC).

METHODSStat3 activity in ESCC cells was inhibited with JAK/Stat3 inhibitors (AG490 or JSI-124). Specific siRNA was used to inhibit the Stat3 expression. Cell apoptosis was detected by flow cytometry. Expression of Mcl-1 protein was determined by Western blotting. Expression of phospho-Stat3 (Tyr705) and myeloid leukemia-1 (Mcl-1) proteins in ESCC tissues was detected by tissue microarray and immunohistochemistry. The relationship between p-Stat3 or Mcl-1 aberrant expression and clinicopatholohical features of ESCC was analyzed. The correlation of their expression was also analyzed.

RESULTSSuppression of the Stat3 signaling activation in ESCC cells led to marked apoptosis, and dramatic reduction of Mcl-1 protein. The positive rate of phospho-Stat3 (Tyr705) expression was 45.0% in 50/111 of the ESCC tissue samples. The lower the degree of tumor differentiation, the higher the positive rate of phospho-Stat3 (Tyr705), showing a significant difference (P = 0.018). The positive rate of Mcl-1 protein expression was 72.1% (80/111), and the lower the degree of tumor differentiation was, the higher there was the positive rate of Mcl-1, with a significant difference (P = 0.026). There was a positive correlation between the expressions of p-Stat3 and Mcl-1 proteins (P = 0.012).

CONCLUSIONSIn a subset of ESCC tissues, p-Stat3 (Tyr705) and Mcl-1 are overexpressed and positively correlated with each other, and both are correlated with tumor differentiation. Persistent activation of Stat3 contributes to apoptotic resistance in ESCC cells, and may be at least partly mediated through upregulation of Mcl-1.

Antineoplastic Agents ; pharmacology ; Apoptosis ; drug effects ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Cell Differentiation ; Cell Line, Tumor ; Cell Survival ; drug effects ; Esophageal Neoplasms ; metabolism ; pathology ; Humans ; Myeloid Cell Leukemia Sequence 1 Protein ; metabolism ; Neoplasm Grading ; Neoplasm Staging ; Phosphorylation ; RNA, Small Interfering ; genetics ; STAT3 Transcription Factor ; antagonists & inhibitors ; genetics ; metabolism ; Tyrphostins ; pharmacology

Animals ; Borrelia burgdorferi ; genetics ; China ; DNA, Bacterial ; genetics ; isolation & purification ; Rodentia ; microbiology ; Sequence Analysis, DNA

Objective To monitor the co-infection status of Borrelia burgdorferi sensu lato (R.b.s.1) and spotted fever group Rickettsia (SFGR) in tourist areas of Heilongjiang province.Methods Polymerase chain reaction (PCR) was used to detect the 5S-23S rRNA intergenic spacer of B.b.s.1 and ompA of SFGR in ticks,dynamically collected from tourist areas of Heilongjiang province in 2010.Amplification products from positive ticks were sequenced,and phylogenetic analysis was conducted by Mega 5.0 software package.Results 849 ticks were collected from two tourist points,with the dominant ticks in Tiger Mountain and Jingpo Lake were Ixodes persulcatus and Haemaphysalis concinna.Regarding the Ixodes persulcatus from Tiger Mountain,the infection rates of B.b.s.1 and SFGR were 26.15％ and 10.05％.The infection rate of SFGR was 13.33％ in Haemaphysalis concinna and the B.b.s.1 was tndiscovered in the same ticks from Jingpo Lake.However,the co-infection could only be detected in Ixodes persulcatus of both tourist areas.Surveillance data showed that the major ticks were more likely to be appeared in July at Tiger Mountain and in June at Jingpo Lake.Data from the sequence analysis on B.b.s.1 showed that the B.b.s.1 in tourist areas could be classified into three different genotypes,other than B.garinii and B.afzelii.We first detected B.valaisiana-like group genotype in northeast of China.Results from the sequence analysis of SFGR positive products showed that the two DNA sequences of newly detected agents were completely the same as Rickettsia sp.HL-93 which was detected in Hulin and Rickettsia sp.H820 found in northeast,China.Conclusion The co-infection of B.b.s.1 and SFGR was detected in ticks from the tourist areas of Heilongjiang province,and data from the sequencing of specific fragment showed that various kinds of genotypes existed in this area.However; the rates of co-infectionitis-different according to environment,time and population that contributed to the kinds of and the index of ticks existed in the surveys points,also the infection rate of the ticks was studied.