Chemotherapy is currently the mainstay of systemic management for triple-negative breast cancer (TNBC), but chemoresistance significantly impacts patient outcomes. Our research indicates that Doxorubicin (Dox)-resistant TNBC cells exhibit increased glycolysis and ATP generation compared to their parental cells, with this metabolic shift contributing to chemoresistance. We discovered that ALKBH3, an m¹A demethylase enzyme, is crucial in regulating the enhanced glycolysis in Dox-resistant TNBC cells. Knocking down ALKBH3 reduced ATP generation, glucose consumption, and lactate production, implicating its involvement in mediating glycolysis. Further investigation revealed that aldolase A (ALDOA), a key enzyme in glycolysis, is a downstream target of ALKBH3. ALKBH3 regulates ALDOA mRNA stability through m¹A demethylation at the 3'-untranslated region (3'UTR). This methylation negatively affects ALDOA mRNA stability by recruiting the YTHDF2/PAN2-PAN3 complex, leading to mRNA degradation. The ALKBH3/ALDOA axis promotes Dox resistance both in vitro and in vivo. Clinical analysis demonstrated that ALKBH3 and ALDOA are upregulated in breast cancer tissues, and higher expression of these proteins is associated with reduced overall survival in TNBC patients. Our study highlights the role of the ALKBH3/ALDOA axis in contributing to Dox resistance in TNBC cells through regulation of ALDOA mRNA stability and glycolysis.

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.

Since December 2019, novel coronavirus pneumonia (NCP) has been reported in Wuhan, Hubei Province, and spreads rapidly to all through Hubei Province and even to the whole country. The virus is 2019 novel coronavirus (2019-nCoV), never been seen previously in human, but all the population is generally susceptible. The virus spreads through many ways and is highly infectious, which brings great difficulties to the prevention and control of NCP. Based on the needs of orthopedic trauma patients for emergency surgery and review of the latest NCP diagnosis and treatment strategy and the latest principles and principles of evidence-based medicine in traumatic orthopedics, the authors put forward this expert consensus to systematically standardize the clinical pathway and protective measures of emergency surgery for orthopedic trauma patients during prevention and control of NCP and provide reference for the emergency surgical treatment of orthopedic trauma patients in hospitals at all levels.

OBJECTIVETo study the effects of two different endodontic sealers on the bond strength of two fiber posts cemented with adhesive resin cement.

METHODSTwenty-eight crownless human maxillary central incisors were prepared with the step-back technique and randomly divided into four groups according endodontic sealer and fiber: Group A, Cortisomol sealer+Matchpost fiber post; Group B, Cortisomol sealer+Macrolock fiber post; Group C, Guttaflow sealer+Matchpost fiber post; Group D, Guttaflow sealer+Macrolock fiber post. One week after fiber posts were bonded, a thin-slice push-out test was performed in a universal machine. Morphologic structure of the root canal dentin surfaces etched and the adhesive interfaces were examined by scanning electron microscopy (SEM).

RESULTSThe bond strengths of 4 groups were (7.06 +/- 3.22), (9.31 +/- 3.61), (6.90 +/- 3.13), (9.71 +/- 3.42) MPa. The bond strengths of group B and D were significantly higher than that of group A and C (P < 0.05). There was no significant difference between group A and C, group B and D (P > 0.05). The cervical third had the highest mean push-out bond strength, next to middle third and apical third (P < 0.01). SEM showed that larger numbers of the dentin tubules were open after the root canals were etched and penetration of resin tags into the dentinal tubules increased in the coronal root region when compared with the apical root region.

CONCLUSIONThe shear bond strength doesn't have relationship with endodontic sealers. The serration significantly increases the retention of fiber post. The bond strengths are different at the three root segment sites.

Dental Bonding ; Dentin ; Dentin-Bonding Agents ; Dimethylpolysiloxanes ; Drug Combinations ; Gutta-Percha ; Humans ; Incisor ; Post and Core Technique ; Resin Cements ; Root Canal Therapy ; Tooth Root ; Zinc Oxide-Eugenol Cement