Objective To analyze the serological survey results of Changsha City in 2016 and 2024, and to investigate the short-term impact of adjusting the immunization program with two doses of mumps containing vaccine on the mumps antibody levels of healthy individuals in Changsha City. Methods Using the relevant data and samples from the mumps serological surveys conducted in 2016 and 2024, serum mumps IgG antibodies were quantitatively detected by enzyme-linked immunosorbent assay. The results from these two surveys were analyzed and compared using SPSS 25.0 software. Results The survey included 1 512 participants in 2016 and 1 398 in 2024. In 2024, both the seropositivity rate and IgG antibody levels against mumps in the healthy population were significantly higher than those in 2016 (all P<0.01). Specifically, the seropositivity rates and IgG antibody levels in the age groups of 8–17 months, 18 months–2 years, 3–4 years, and 5–9 years were significantly higher in 2024 compared to 2016 (all P < 0.001). However, among the participants in 2024, the 15–19 years age group showed relatively low seropositivity rate and IgG antibody levels. Conclusion Following the adjustment of the two-dose MuCV immunization strategy, the mumps IgG antibody levels among children aged 8 months to 9 years in Changsha have increased. It is necessary to continue implementing the routine two-dose MuCV vaccination strategy and to explore the necessity of strengthening and supplementing immunization for key groups, including the high-risk 5–9-year-old population and the immunologically vulnerable 15–19-year-old population.

OBJECTIVE: To compare the expression of matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 of nasopharyngeal carcinoma at Xi'an and Shenzhen area. METHOD: The protein expression of MMP 9 and TIMP 1 were studied by immunohistochemistry staining in 21 cases nasopharyngeal carcinoma of Xi'an and 22 cases of Shenzhen. RESULT: The expression positive rates of MMP-9 in nasopharyngeal carcinoma tissues at Xi'an and Shenzhen were 61.90% and 68.18%. The positive rates of TIMP-1 in nasopharyngeal carcinoma tissues at two regions were 28.57% and 31.82%. The expression between MMP-9 and TIMP-1 were negative relationship either Xi'an or Shenzhen. The expression of MMP-9 was positively correlated to lymph node metastasis, and TIMP-1 was no relationship with lymph node metastasis. The expression of MMP-9 and TIMP-1 was no difference between Xi'an and Shenzhen. CONCLUSION The expression of MMP-9 and TIMP-1 in nasopharyngeal carcinoma 2 tissues was no difference between Xi'an and Shenzhen. Increase of MMP-9 play an important role in progression and metastasis of nasopharyngeal carcinoma.
Adolescent ; Adult ; Aged ; Carcinoma ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Child ; China ; Female ; Humans ; Male ; Matrix Metalloproteinase 9 ; metabolism ; Middle Aged ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; metabolism ; pathology ; Tissue Inhibitor of Metalloproteinase-1 ; metabolism ; Young Adult