Objective To observe hierarchical differences in brain functional networks in unilateral mesial temporal lobe epilepsy(mTLE)patients with different outcomes after surgery.Methods A total of 69 unilateral mTLE patients who underwent resection of epileptogenic lesion on the affected side were retrospectively enrolled.Based on Engel classification 1 year after surgery,the patients were divided into seizure free(SF)group and non-seizure free(NSF)group.Functional connectivity gradient analysis was employed to extract principal gradient explaining the highest variance on preoperative resting-state functional MRI(rs-fMRI),then the whole-brain gradient characteristics and principal gradient values within specific functional networks were compared between groups.Results Principal gradient connected default mode network(DMN)with sensorimotor network(SMN)along a continuous axis.Compared to SF group,NSF group showed a contracted gradient range at both ends(DMN and SMN)of the functional network and weakened hierarchical differentiation,and principal gradient value of DMN was higher,while that of SMN was lower than those in SF group(both P＜0.05).Conclusion Hierarchical differences in brain functional networks in unilateral mTLE patients with different outcomes after surgery mainly present as enhanced DMN and weakened SMN in NSF ones,and the latter two might serve as important neuroimaging markers for evaluating postoperative seizure recurrence.

Objective:To conduct an occupational hygiene investigation and simulated sampling and testing on welding positions in a company in a certain province, and to perform a simulated analysis of the concentration of occupational hazard factors in workers suspected of occupational asthma.Methods:In November 2023, the investigation and analysis of workers' exposure to occupational hazard factors were carried out through occupational hygiene surveys, laboratory simulated sampling and testing, and a combination of qualitative and quantitative detection methods.Results:Toluene diisocyanate (TDI) was present in the workplace air of the employer. TDI was detected in the raw materials provided by the workers. Laboratory simulations of the production process and working conditions at the position were conducted with sampling at different distances, revealing a maximum TDI concentration of 0.49 mg/m 3 and a minimum concentration of 0.01 mg/m 3. The results showed that the closer the sampling distance was to the breathing zone, the more significantly the TDI concentration increased. The laboratory simulated sampling and testing results were highly consistent with the on-site sampling and testing results from a testing agency in Beijing. Conclusion:The occupational hazard factor TDI is present in the workers' workplace, and TDI may be the cause of the suspected occupational asthma in the workers.

Oral rare diseases are a group of disorders characterized by distinct oral and craniofacial manifestations. These conditions are featured by low prevalence, high genetic and clinical heterogeneity, and great challenges in diagnosis and treatment. They can severely impair the development and physiological functions, posing substantial risks to both physical and mental health. In recent years, rapid advancements in genetics, molecular biology, and regenerative medicine have led to notable progress in the research of oral rare diseases. This review summarizes the current research landscape in this field, focusing on causative genes and mechanisms, diagnostic technologies, therapeutic approaches, clinical research, and digital innovations. It also discusses the existing challenges, aiming to promote further research and clinical translation in the field of oral rare diseases.

Oral rare diseases are a group of disorders characterized by distinct oral and craniofacial manifestations. These conditions are featured by low prevalence, high genetic and clinical heterogeneity, and great challenges in diagnosis and treatment. They can severely impair the development and physiological functions, posing substantial risks to both physical and mental health. In recent years, rapid advancements in genetics, molecular biology, and regenerative medicine have led to notable progress in the research of oral rare diseases. This review summarizes the current research landscape in this field, focusing on causative genes and mechanisms, diagnostic technologies, therapeutic approaches, clinical research, and digital innovations. It also discusses the existing challenges, aiming to promote further research and clinical translation in the field of oral rare diseases.

Early correction of childhood malocclusion is timely managing morphological, structural, and functional abnormalities at different dentomaxillofacial developmental stages. The selection of appropriate imaging examination and comprehensive radiological diagnosis and analysis play an important role in early correction of childhood malocclusion. This expert consensus is a collaborative effort by multidisciplinary experts in dentistry across the nation based on the current clinical evidence, aiming to provide general guidance on appropriate imaging examination selection, comprehensive and accurate imaging assessment for early orthodontic treatment patients.
Humans ; Malocclusion/diagnostic imaging* ; Child ; Consensus

Ankylosing spondylitis (AS) is linked to an increased prevalence of myocardial infarction (MI). However, research dedicated to elucidating the pathogenesis of AS-MI is lacking. In this study, we explored the biomarkers for enhancing the diagnostic and therapeutic efficiency of AS-MI. Datasets were obtained from the Gene Expression Omnibus database. We employed weighted gene co-expression network analysis and machine learning models to screen hub genes. A receiver operating characteristic curve and a nomogram were designed to assess diagnostic accuracy. Gene set enrichment analysis was conducted to reveal the potential function of hub genes. Immune infiltration analysis indicated the correlation between hub genes and the immune landscape. Subsequently, we performed single-cell analysis to identify the expression and subcellular localization of hub genes. We further constructed a transcription factor (TF)-microRNA (miRNA) regulatory network. Finally, drug prediction and molecular docking were performed. S100A12 and MCEMP1 were identified as hub genes, which were correlated with immune-related biological processes. They exhibited high diagnostic value and were predominantly expressed in myeloid cells. Furthermore, 24 TFs and 9 miRNA were associated with these hub genes. Enzastaurin, meglitinide, and nifedipine were predicted as potential therapeutic agents. Our study indicates that S100A12 and MCEMP1 exhibit significant potential as biomarkers and therapeutic targets for AS-MI, offering novel insights into the underlying etiology of this condition.
Humans ; Spondylitis, Ankylosing/complications* ; Systems Biology/methods* ; Myocardial Infarction/diagnosis* ; Biomarkers/metabolism* ; MicroRNAs/genetics* ; Gene Regulatory Networks ; Gene Expression Profiling ; Machine Learning

Objective:To test the difficulty, discrimination, and reliability of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire.Methods:Two researchers independently translated the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire and cross-checked it to form a Chinese version of the questionnaire. The Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire consists of 24 items, with correct answers scoring one point and incorrect answers scoring zero points, with a total score of 24 points. Convenience sampling was used to select ICU nurses from 14 GradeⅢ Class A hospitals in five provinces/autonomous regions and two municipalities in China for the survey between April and July 2023. The difficulty index, discrimination index, and Cronbach's α coefficient of the questionnaire were analyzed.Results:A total of 1 121 questionnaires were distributed, with 1 020 valid responses, yielding a valid response rate of 90.99%. The mean score of the 1 020 ICU nurses on the Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire was (16.10±5.58) , with a minimum score of 4.00 and a maximum score of 24.00. The Cronbach's α coefficient of the Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire was 0.91. The questionnaire's overall difficulty and discrimination indexes were 0.67 and 0.59, respectively.Conclusions:The Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire has appropriate difficulty, moderate discrimination, and strong reliability, making it a valuable tool for assessing ICU nurses' knowledge of pressure injury-related topics.

Objective To observe hierarchical differences in brain functional networks in unilateral mesial temporal lobe epilepsy(mTLE)patients with different outcomes after surgery.Methods A total of 69 unilateral mTLE patients who underwent resection of epileptogenic lesion on the affected side were retrospectively enrolled.Based on Engel classification 1 year after surgery,the patients were divided into seizure free(SF)group and non-seizure free(NSF)group.Functional connectivity gradient analysis was employed to extract principal gradient explaining the highest variance on preoperative resting-state functional MRI(rs-fMRI),then the whole-brain gradient characteristics and principal gradient values within specific functional networks were compared between groups.Results Principal gradient connected default mode network(DMN)with sensorimotor network(SMN)along a continuous axis.Compared to SF group,NSF group showed a contracted gradient range at both ends(DMN and SMN)of the functional network and weakened hierarchical differentiation,and principal gradient value of DMN was higher,while that of SMN was lower than those in SF group(both P＜0.05).Conclusion Hierarchical differences in brain functional networks in unilateral mTLE patients with different outcomes after surgery mainly present as enhanced DMN and weakened SMN in NSF ones,and the latter two might serve as important neuroimaging markers for evaluating postoperative seizure recurrence.

Objective:To test the difficulty, discrimination, and reliability of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire.Methods:Two researchers independently translated the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire and cross-checked it to form a Chinese version of the questionnaire. The Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire consists of 24 items, with correct answers scoring one point and incorrect answers scoring zero points, with a total score of 24 points. Convenience sampling was used to select ICU nurses from 14 GradeⅢ Class A hospitals in five provinces/autonomous regions and two municipalities in China for the survey between April and July 2023. The difficulty index, discrimination index, and Cronbach's α coefficient of the questionnaire were analyzed.Results:A total of 1 121 questionnaires were distributed, with 1 020 valid responses, yielding a valid response rate of 90.99%. The mean score of the 1 020 ICU nurses on the Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire was (16.10±5.58) , with a minimum score of 4.00 and a maximum score of 24.00. The Cronbach's α coefficient of the Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire was 0.91. The questionnaire's overall difficulty and discrimination indexes were 0.67 and 0.59, respectively.Conclusions:The Chinese version of the ICU Nurse Pressure Injury Prevention and Care Knowledge Questionnaire has appropriate difficulty, moderate discrimination, and strong reliability, making it a valuable tool for assessing ICU nurses' knowledge of pressure injury-related topics.

Objective:To conduct an occupational hygiene investigation and simulated sampling and testing on welding positions in a company in a certain province, and to perform a simulated analysis of the concentration of occupational hazard factors in workers suspected of occupational asthma.Methods:In November 2023, the investigation and analysis of workers' exposure to occupational hazard factors were carried out through occupational hygiene surveys, laboratory simulated sampling and testing, and a combination of qualitative and quantitative detection methods.Results:Toluene diisocyanate (TDI) was present in the workplace air of the employer. TDI was detected in the raw materials provided by the workers. Laboratory simulations of the production process and working conditions at the position were conducted with sampling at different distances, revealing a maximum TDI concentration of 0.49 mg/m 3 and a minimum concentration of 0.01 mg/m 3. The results showed that the closer the sampling distance was to the breathing zone, the more significantly the TDI concentration increased. The laboratory simulated sampling and testing results were highly consistent with the on-site sampling and testing results from a testing agency in Beijing. Conclusion:The occupational hazard factor TDI is present in the workers' workplace, and TDI may be the cause of the suspected occupational asthma in the workers.