Parkinson's disease(PD)is a common progressive neurodegenerative disease mainly affecting the motor system.Various genetic factors and cellular mechanisms underlying PD have recently been discovered.Emerging evidence suggests that epigenetic modifications play a very important role in the pathogenesis,prevention,and treatment of PD.Epigenetic modification mediates genetic and environmental interactions mainly through complex interactions of DNA methylation,histone modification,and non-coding RNA,thereby affecting expression in the absence of changes in DNA sequence.In this review,we summarize the epigenetic modification mechanisms involved in the pathogenesis of Parkinson's disease.In this review,we summarize the epigenetic modification mechanisms involved in the pathogenesis of Parkinson's disease.Recent studies have found that traditional Chinese medicine can participate in the regulation of abnormal epigenetic modifications in the treatment of PD.Traditional Chinese medicine benefits from its multi-level and multi-target regulatory effects,and various traditional Chinese medicine monomers,compound prescriptions,and techniques have been evaluated,confirming that this is a promising approach for improving symptoms in PD.This review summarizes the mechanisms by which epigenetic modifications contribute to P D,explores the role of traditional Chinese medicine,and provides new ideas for clinical treatment and drug development in PD through epigenetic intervention.

Objective:To deeply understand the experience of neurosurgery nurses on the lack of perioperative nursing for intracranial tumors patients, so as to provide reference for reducing the lack of nursing and improving the quality of nursing and patients′ medical experience.Methods:From December 2023 to February 2024, the neurosurgical nurses of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology were selected by the purpose sampling method with maximum differentiation. Semi-structured interviews were conducted with phenomenological research methods. The interview data were transcribed, coded, categorized and described.Results:A total of 17 neurosurgical nurses were enrolled, including 6 males and 11 females, aged 24-45(33.29 ± 6.74) years. Three themes of perioperative nursing lack experience of neurosurgery nurses were obtained, including nursing lack experience (lack of safety nursing, lack of timely nursing, lack of high efficiency nursing, lack of effectiveness nursing, lack of fair nursing, and lack of patient-centered), the physical and mental effects of nursing lack on nurses (impacts of lack of nursing on nurses′ physiology and psychology), and reasons for lack of nursing (personnel, equipment, materials, organization management, environment).Conclusions:Neurosurgical nurses lack of perioperative nursing for patients with intracranial tumor, which has a certain impact on the physical and mental health of nurses, suggesting that neurosurgical nurses should attach great importance to the lack of nursing for patients with intracranial tumor and formulate a scientific and effective management system.

Parkinson's disease(PD)is a common progressive neurodegenerative disease mainly affecting the motor system.Various genetic factors and cellular mechanisms underlying PD have recently been discovered.Emerging evidence suggests that epigenetic modifications play a very important role in the pathogenesis,prevention,and treatment of PD.Epigenetic modification mediates genetic and environmental interactions mainly through complex interactions of DNA methylation,histone modification,and non-coding RNA,thereby affecting expression in the absence of changes in DNA sequence.In this review,we summarize the epigenetic modification mechanisms involved in the pathogenesis of Parkinson's disease.In this review,we summarize the epigenetic modification mechanisms involved in the pathogenesis of Parkinson's disease.Recent studies have found that traditional Chinese medicine can participate in the regulation of abnormal epigenetic modifications in the treatment of PD.Traditional Chinese medicine benefits from its multi-level and multi-target regulatory effects,and various traditional Chinese medicine monomers,compound prescriptions,and techniques have been evaluated,confirming that this is a promising approach for improving symptoms in PD.This review summarizes the mechanisms by which epigenetic modifications contribute to P D,explores the role of traditional Chinese medicine,and provides new ideas for clinical treatment and drug development in PD through epigenetic intervention.

Objective:To deeply understand the experience of neurosurgery nurses on the lack of perioperative nursing for intracranial tumors patients, so as to provide reference for reducing the lack of nursing and improving the quality of nursing and patients′ medical experience.Methods:From December 2023 to February 2024, the neurosurgical nurses of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology were selected by the purpose sampling method with maximum differentiation. Semi-structured interviews were conducted with phenomenological research methods. The interview data were transcribed, coded, categorized and described.Results:A total of 17 neurosurgical nurses were enrolled, including 6 males and 11 females, aged 24-45(33.29 ± 6.74) years. Three themes of perioperative nursing lack experience of neurosurgery nurses were obtained, including nursing lack experience (lack of safety nursing, lack of timely nursing, lack of high efficiency nursing, lack of effectiveness nursing, lack of fair nursing, and lack of patient-centered), the physical and mental effects of nursing lack on nurses (impacts of lack of nursing on nurses′ physiology and psychology), and reasons for lack of nursing (personnel, equipment, materials, organization management, environment).Conclusions:Neurosurgical nurses lack of perioperative nursing for patients with intracranial tumor, which has a certain impact on the physical and mental health of nurses, suggesting that neurosurgical nurses should attach great importance to the lack of nursing for patients with intracranial tumor and formulate a scientific and effective management system.

Objective:To investigate the effect of circular RNA(cireRNA)pumilio RNA binding family member 1(PUM1)on the proliferation,migration,invasion and apoptosis of endometrial cancer(EC)Ishikawa cells by regulating the miR-337-3p/nucleophosmin 1(NPM1)axis.Methods:Ishikawa cells were selected and plasmid sh-circPUM1 and its negative control(sh-NC),anti-miR-337-3p and its negative control(anti-NC)were transfected into Ishikawa cells by RNA interference.The experiment cells were divided into the control group(non-transfected cells),sh-NC group,sh-circPUM1 group,sh-circPUM1+anti-NC group and sh-circPUM1+anti-miR-337-3p group.The qPCR method was applied to detect the expressions of circPUM1,miR-337-3p,and NPM1 mRNA in Ishikawa cells in each group.CCK-8 method,EdU staining method,Transwell assay,and flow cytometry were applied respectively to detect the effects of knocking down circPUM1 on the proliferation,migration,invasion and apoptosis of Ishikawa cells.Western blot was applied to detect the changes in the expressions of PCNA,NPM1,MMP-9,SNAIL,E-cadherin,BAX and C-caspase-3 proteins in Ishikawa cells.Dual luciferase reporter gene experiment was applied to verify the targeting relationship between circPUM1 and miR-337-3p,and between miR-337-3p and NPM1.Results:Cell proliferation ability,EdU positive cell rate,migration and invasion numbers,circPUM1,NPM1 mRNA and protein,the expressions of PCNA,NPM1,MMP-9 and SNAIL protein in Ishikawa cells in the sh-circPUM1 group were significantly lower than those in the sh-NC group and Control group(all P<0.05);the apoptosis rate,the expressions of miR-337-3p,E-cadherin,BAX,and C-caspase-3 proteins were significantly higher than those in the sh-NC group and the Control group(all P<0.05).Compared with those in the sh-circPUM1 group and the sh-circPUM1+anti-NC group,the apoptosis rate,miR-337-3p,the expressions of E-cadherin,BAX,and C-caspase-3 proteins in the sh-circPUM1+anti-miR-337-3p group were significantly lower(all P<0.05);Cell proliferation ability,EdU positive cell rate,migration and invasion numbers,NPM1 mRNA and protein,the expressions of PCNA,NPM1,MMP-9,and SNAIL proteins were significantly higher(all P<0.05).CircPUM1 might target and negatively regulate miR-337-3p,and miR-337-3p might target and negatively regulate NPM1.Conclusion:Knocking down circPUM1 can inhibit the malignant biological behavior of Ishikawa cells,which might be achieved by targeting the miR-337-3p/NPM1 axis.

AIM:To investigate the effect of hypericin(Hyp)on the heart of rats with myocardial ischemia-re-perfusion injury(MIRI),and to explore its mechanism.METHODS:Thirty SPF male SD rats were divided into 5 groups:sham group,MIRI group,low-dose Hyp(L-Hyp)group(MIRI+L-Hyp group),high-dose Hyp(H-Hyp)group(MIRI+H-Hyp group),and positive control trimetazidine(TMZ)group(MIRI+TMZ group),with 6 rats in each group.Apart from the sham group,the MIRI model was established by ligating the anterior descending branch of the left coronary artery and then recanalizing it in the remaining four groups of rats.The success of the modeling was determined by monitor-ing the electrocardiogram.We assessed the cardiac function in rats using echocardiography.TTC staining was employed to measure the area of myocardial infarction in rats,and HE staining was utilized to observe the morphological traits of rat myocardium.We assayed the activities of lactate dehydrogenase(LDH)and superoxide dismutase(SOD)and the levels of malondialdehyde(MDA)in rat serum using biochemical kits.ELISA kits were applied to assess the contents of cardiac troponin I(cTnI)and reactive oxygen species(ROS)in rat serum.Western blot analysis was perfomed to measure the pro-tein expression levels of AMPK,p-AMPK,Nrf2,and HO-1 in rat myocardial tissues.RESULTS:The rats in MIRI group exhibited increased myocardial tissue injury,larger myocardial infarction areas,decreased left ventricular ejection fraction(LVEF),and reduced left ventricular fractional shortening(LVFS)compared with sham group,as shown by echo-cardiography.Additionally,there were increases in LDH activity,cTnI,MDA and ROS levels,along with significant de-creases in SOD activity,and p-AMPK,Nrf2 and HO-1 protein levels(P<0.05).Compared with MIRI group,the rats in MIRI+L-Hyp,MIRI+H-Hyp and MIRI+TMZ groups showed decreased myocardial histopathological damage and reduced myocardial tissue infarction area,increased LVEF and LVFS,and lowered serum levels of LDH activity,cTnI,MDA and ROS,while SOD activity,p-AMPK,Nrf2 and HO-1 protein levels were elevated(P<0.05).CONCLUSION:Hypericin attenuates myocardial ischemia-reperfusion injury in rats,possibly by modulating the AMPK/Nrf2/HO-1 signaling path-way.

Objective:To develop a transitional care practice protocol for neurosurgical intensive care unit (ICU) patients based on the Secure-Encourage-Collaborate (SEC) model and to explore its effectiveness, providing a reference for clinical practice.Methods:Through literature analysis, expert verification in our hospital, core group discussions, and the Delphi expert consultation method, a transitional care practice protocol for neurosurgical ICU patients based on the SEC model was developed. A convenience sampling method was used to select 160 patients admitted to the Neurosurgical ICU of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, from March 2022 to October 2023. Eighty patients admitted from March to December 2022 were designated as the control group, and eighty patients admitted from January to October 2023 were designated as the experimental group. The control group received the routine post-discharge follow-up care protocol, while the experimental group received the transitional care practice protocol based on the SEC model. The ICU quality control indicators, rehabilitation outcomes, adverse events were compared between the two groups.Results:The experimental group had significantly shorter ICU stay and total hospitalization days compared to the control group, and fewer cases of readmission within 48 hours after ICU discharge ( P<0.05). The experimental group showed lower pain scores, higher scores for self-care in daily activities, shorter time to first ambulation after ICU discharge, and longer daily ambulation time within the first week post-discharge compared to the control group, with statistically significant differences ( P<0.05). No statistically significant differences were observed between the two groups in the incidence of stage II or higher pressure injuries, falls/bed falls, unplanned extubations, aspiration, and lower limb deep vein thrombosis ( P>0.05) . Conclusions:The transitional care practice protocol for neurosurgical ICU patients based on the SEC model can effectively improve the quality of care.

Objective:To develop a transitional care practice protocol for neurosurgical intensive care unit (ICU) patients based on the Secure-Encourage-Collaborate (SEC) model and to explore its effectiveness, providing a reference for clinical practice.Methods:Through literature analysis, expert verification in our hospital, core group discussions, and the Delphi expert consultation method, a transitional care practice protocol for neurosurgical ICU patients based on the SEC model was developed. A convenience sampling method was used to select 160 patients admitted to the Neurosurgical ICU of Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, from March 2022 to October 2023. Eighty patients admitted from March to December 2022 were designated as the control group, and eighty patients admitted from January to October 2023 were designated as the experimental group. The control group received the routine post-discharge follow-up care protocol, while the experimental group received the transitional care practice protocol based on the SEC model. The ICU quality control indicators, rehabilitation outcomes, adverse events were compared between the two groups.Results:The experimental group had significantly shorter ICU stay and total hospitalization days compared to the control group, and fewer cases of readmission within 48 hours after ICU discharge ( P<0.05). The experimental group showed lower pain scores, higher scores for self-care in daily activities, shorter time to first ambulation after ICU discharge, and longer daily ambulation time within the first week post-discharge compared to the control group, with statistically significant differences ( P<0.05). No statistically significant differences were observed between the two groups in the incidence of stage II or higher pressure injuries, falls/bed falls, unplanned extubations, aspiration, and lower limb deep vein thrombosis ( P>0.05) . Conclusions:The transitional care practice protocol for neurosurgical ICU patients based on the SEC model can effectively improve the quality of care.

Objective:To analyze the clinical epidemiological characteristics and the prognostic risk factors of patients with hemorrhagic fever with renal syndrome (HFRS).Methods:A total of 2 245 HFRS patients who were admitted to the Second Affiliated Hospital of Air Force Medical University from September 2008 to December 2021 were enrolled. Clinical epidemiological data (including gender, age, onset season, onset region, case fatality rate, et al) of HFRS patients were analyzed. The clinical epidemiological characteristics of patients with HFRS in the 2008 to 2012, 2013 to 2017, and 2018 to 2021 groups were compared. Statistical comparisons were performed using chi-square test. The Bonferroni adjusted P-value method was used for pairwise comparisons between groups, and logistic regression analysis was used to screen and evaluate the risk factors associated with the prognosis of HFRS patients. Results:The age of 2 245 HFRS patients was (42.3±15.9) years old. Most of them were male (79.24%(1 779/2 245)), and the main incidence area was Xi′an City (69.53%(1 561/2 245)). There were 132 deaths with an overall case fatality rate of 5.88%. There were 1 088 patients (48.46%) from 2008 to 2012, 647 patients (28.82%) from 2013 to 2017, and 510 patients (22.72%) from 2018 to 2021, with a mortality rate of 7.17%(78/1 088), 5.10%(33/647) and 4.12%(21/510), respectively. From 2008 to 2021, both the number of HFRS cases and the case fatality rate had shown a fluctuating downward trend. There were significant differences in case fatality rate, age distribution, onset season, and onset region among patients in the different year groups ( χ2=6.84, 49.22, 83.47 and 19.29, respectively, all P<0.05). The results of pairwise comparisons showed that the proportion of patients aged >60 years in the 2018 to 2021 group (23.33%(119/510)) was higher than those in the 2008 to 2012 group (12.13%(132/1 088)) and the 2013 to 2017 group (12.36%(80/647)), and the differences were statistically significant (both P<0.05). The proportions of patients at large peak (October to December) were 62.35%(318/510) in the 2018 to 2021 group and 56.26%(364/647) in the 2013 to 2017 group, which were both lower than that in the 2008 to 2012 group (75.18%(818/1 088)), and the differences were both statistically significant (both P<0.05). The case fatality rate of patients aged >60 years was 9.67%(32/331), which was higher than those of patients aged <30 years (2.86%(16/559)) and patients aged 30 to 60 years (6.20%(84/1 355)), with statistically significant differences (both P<0.05). Univariate analysis showed that age 30 to 60 years, age >60 years, smoking, complicated with hypertension, hypotensive shock and hypoxemia were significantly correlated with the prognosis of HFRS patients (odds ratio ( OR)=2.243, 3.632, 1.484, 3.532, 79.422 and 143.955, respectively, all P<0.05). The results of multivariate logistic regression analysis indicated that complicated with hypertension ( OR=2.467, P=0.004), hypotensive shock ( OR=11.658, P=0.001), and hypoxemia ( OR=67.767, P<0.001) were the independent risk factors affecting the prognosis of HFRS patients. Conclusions:The prevalence of HFRS has shown new changing characteristics from 2008 to 2021. The numbers of HFRS patients and the case fatality rates show a downward trend, and the proportion of HFRS patients aged >60 years increases. Complicated with hypertension, hypotensive shock and development with hypoxemia are the independent risk factors for the prognosis of HFRS.

Objective:To analysis effectiveness of the "14 plus 7 day quarantine" and "nucleic acid plus total antibody testing" strategy (combined screening strategy) for screenin the imported patients with COVID-19 in Xiamen.Methods:The study populations were overseas travelers arriving in Xiamen from March 17 to December 31, 2020, and overseas travelers who had quarantine outside Xiamen for less than 21 days from July 18 to December 31, 2020. Data were collected and analyzed on the timing of detection, pathways, and test results of the imported patients with COVID-19 after implementing combined screening strategy.Results:A total of 304 imported patients with COVID-19 were found from 174 628 overseas travelers and 943 overseas travelers from other cities. A total of 163 cases (53.6%) were diagnosed by multitime, multisite intensive nucleic acid testing after positive finding in total antibody testing. Among them, 27 (8.9%) were first positive for nucleic acid in 14 plus 7 day quarantine and 136 were first positive for nucleic acid in 14-day quarantine. Only 8 of these individuals were tested positive for nucleic acid after positive total antibody testing. The other 128 individuals were tested positive for nucleic acid after being negative for average 2.3 times (maximum of 6 times). Aditional 155 cases might be detected by using the combined "14 plus 7 day quarantine" and " nucleic acid plus total antibody testing" strategy compared with "14-day quarantine and nucleic acid testing" strategy, accounting for 51.0% of the total inbound infections. So the combined screening strategy doubled the detection rate for imported patients with COVID-19. No second-generation case caused by overseas travelers had been reported in Xiamen as of February 26, 2021.Conclusions:Xiamen's combined screening strategy can effectively screen the imported patients with COVID-19 who were first positive for nucleic acid after 14 day quarantine. Compared with "14 day quarantine and nucleic acid testing", the combined screening strategy improved detection rate and further reduced the risk of the secondary transmission caused by the imported patients with COVID-19.