Objective To optimize the platelet enrichment method,and to analyze the concentration changes of key molecules in platelet-rich plasma(PRP)before and after activation,as well as the impact of its activated products on the proliferation of rat endothelial progenitor cells.Methods The tube double-centrifu-gation method was employed to optimize platelet enrichment,and the platelet count in the enriched PRP was measured.ELISA was used to detect the concentration changes of vascular endothelial growth factor(VEGF),endostatin(ES),and P-selectin(CD62P)in PRP before and after activation.The PRP was activated by using liquid nitrogen freeze-thaw method,and the effect of its activated products on the proliferation of rat endothelial progenitor cells was evaluated by using the methyl thiazolyl tetrazolium(MTT)assay.Results The optimal enrichment coefficient of platelets achieved by the double-centrifugation method was 4.63.After low-speed,long-duration double centrifugation,the platelet count was highest in the upper layer of the buffy coat.For PRP with a platelet count of 500× 109/L obtained by machine collection,the VEGF con-centrations before and after activation were(3 418.12±488.80)pg/mL and(4 530.04±308.30)pg/mL,re-spectively,the ES concentrations were(6 168.98±253.22)pg/mL and(6 594.65±82.47)pg/mL,respec-tively,the CD62P concentrations were(6 678.23±324.15)pg/mL and(17 630.53±746.24)pg/mL,respec-tively,statistically significant differences were observed in the above indicators before and after activation(P＜0.01).The activated PRP was diluted in a gradient manner by using a specialized culture medium for en-dothelial progenitor cells.MTT assay results indicated that,in the basal medium,the optimal volume fraction for promoting endothelial progenitor cell proliferation was 0.25％after 48 hours of culture;in the complete medium,the optimal volume fractions for promoting endothelial progenitor cell proliferation were 0.062 5％after 24 hours and 0.125％after 48 hours.Conclusion The concentrations of VEGF,ES,and CD62P in the optimized,enriched PRP exhibited significant changes before and after activation.The optimal volume fraction for promoting endothelial progenitor cell proliferation in the basal medium was 0.25％.

Objective:To explore the application effect of Kaitianmen point massage on pain, faligue, anxiety and depression in patients with rheumatoid arthritis.Methods:This study was a randomized controlled study, a total of 80 patients with rheumatoid arthritis who were admitted to Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine from May to November 2023 were selected as the research objects. The control group was given routine treatment and nursing, and the experimental group was given Kaitianmen acupoint massage on the basis of routine treatment and nursing. The differences in pain score, fatigue score, anxiety and depression score before and after intervention were compared between the two groups.Results:In the final, there were 37 cases in the experimental group and 36 cases in the control group completed the study. Before intervention, there was no statistically significant difference in pain scores, fatigue scores, anxiety and depression scores between the two groups (all P>0.05). After four weeks of intervention, the pain score of the experimental group was (2.06 ± 0.35), and that of the control group was (3.72 ± 0.59), the difference was statistically significant ( t=14.57, P<0.05). After four weeks of intervention, the fatigue score of the experimental group was (20.11 ± 8.03), and that of the control group was (25.02 ± 8.32), the difference was statistically significant ( t=2.55, P<0.05). After four weeks of intervention, the anxiety score of the experimental group was (48.33 ± 6.13), which was significantly different from (53.99 ± 4.91) of the control group ( t=4.35, P<0.05), and the depression score of the experimental group was (49.99 ± 6.33), compared with the (54.86 ± 4.83) of control group, the difference was statistically significant ( t=3.69, P<0.05). Conclusions:Kaitianmen acupoint massage can relieve limb pain, fatigue, anxiety and depression in patients with rheumatoid arthritis, which is worthy of clinical promotion.

Modern Chinese dental societies have been a crucial part of the progress of stomatology in China, serving as both witnesses and key drivers of its development. By reviewing historical materials related to these societies, this paper focuses on analyzing their establishment background, developmental stages, overall scale, and major activities. The findings reveal that dental societies exhibited regional, phased, professional, and academic characteristics. Furthermore, the study highlights their contributions to strengthening dental education, promoting standardization within the industry, facilitating academic exchanges, and advocating for the integration of oral and systemic health. This research enriches the study of modern Chinese stomatology history.

Objective To investigate current status in nasal injury caused by nasal continuous positive airway pressure(nCPAP)in neonates in the neonatal intensive care unit(NICU)and its influencing factors so as to provide a basis for clinical nursing.Methods Data between October 2022 and March 2024 were collected from 268 neonates who underwent nCPAP in NICU of our hospital.The number of newborn nasal injury was recorded and multivariate logistic regression analysis was used to explore the risk factors associated with nasal injury caused by nCPAP in NICU neonates.Results The incidence of nasal injury in the 268 neonates with nCPAP was 19.40%(52/268).Risk factors that associated with nasal injury included gestational age≤32 weeks,birth weight<1500g,frequency of sedative use within 24 hours≥2 times,forced body position,incubator humidity>65%,lethargy/coma,history of invasive ventilation,neonatal skin risk assessment scale score≥13 points before nCPAP,and with nCPAP≥8 days(all P<0.05).Conclusion The incidence of nasal injury in NICU neonates with nCPAP is high with many influencing factors.Preventive measures should be taken according to the identified risk factors in order to reduce the incidence of nasal injury in neonates with nCPAP.

Modern Chinese dental societies have been a crucial part of the progress of stomatology in China, serving as both witnesses and key drivers of its development. By reviewing historical materials related to these societies, this paper focuses on analyzing their establishment background, developmental stages, overall scale, and major activities. The findings reveal that dental societies exhibited regional, phased, professional, and academic characteristics. Furthermore, the study highlights their contributions to strengthening dental education, promoting standardization within the industry, facilitating academic exchanges, and advocating for the integration of oral and systemic health. This research enriches the study of modern Chinese stomatology history.

Objective To investigate current status in nasal injury caused by nasal continuous positive airway pressure(nCPAP)in neonates in the neonatal intensive care unit(NICU)and its influencing factors so as to provide a basis for clinical nursing.Methods Data between October 2022 and March 2024 were collected from 268 neonates who underwent nCPAP in NICU of our hospital.The number of newborn nasal injury was recorded and multivariate logistic regression analysis was used to explore the risk factors associated with nasal injury caused by nCPAP in NICU neonates.Results The incidence of nasal injury in the 268 neonates with nCPAP was 19.40%(52/268).Risk factors that associated with nasal injury included gestational age≤32 weeks,birth weight<1500g,frequency of sedative use within 24 hours≥2 times,forced body position,incubator humidity>65%,lethargy/coma,history of invasive ventilation,neonatal skin risk assessment scale score≥13 points before nCPAP,and with nCPAP≥8 days(all P<0.05).Conclusion The incidence of nasal injury in NICU neonates with nCPAP is high with many influencing factors.Preventive measures should be taken according to the identified risk factors in order to reduce the incidence of nasal injury in neonates with nCPAP.

Objective:To explore the application effect of Kaitianmen point massage on pain, faligue, anxiety and depression in patients with rheumatoid arthritis.Methods:This study was a randomized controlled study, a total of 80 patients with rheumatoid arthritis who were admitted to Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine from May to November 2023 were selected as the research objects. The control group was given routine treatment and nursing, and the experimental group was given Kaitianmen acupoint massage on the basis of routine treatment and nursing. The differences in pain score, fatigue score, anxiety and depression score before and after intervention were compared between the two groups.Results:In the final, there were 37 cases in the experimental group and 36 cases in the control group completed the study. Before intervention, there was no statistically significant difference in pain scores, fatigue scores, anxiety and depression scores between the two groups (all P>0.05). After four weeks of intervention, the pain score of the experimental group was (2.06 ± 0.35), and that of the control group was (3.72 ± 0.59), the difference was statistically significant ( t=14.57, P<0.05). After four weeks of intervention, the fatigue score of the experimental group was (20.11 ± 8.03), and that of the control group was (25.02 ± 8.32), the difference was statistically significant ( t=2.55, P<0.05). After four weeks of intervention, the anxiety score of the experimental group was (48.33 ± 6.13), which was significantly different from (53.99 ± 4.91) of the control group ( t=4.35, P<0.05), and the depression score of the experimental group was (49.99 ± 6.33), compared with the (54.86 ± 4.83) of control group, the difference was statistically significant ( t=3.69, P<0.05). Conclusions:Kaitianmen acupoint massage can relieve limb pain, fatigue, anxiety and depression in patients with rheumatoid arthritis, which is worthy of clinical promotion.

ObjectiveTo investigate the risk factors for early tumor recurrence after laparoscopic pancreaticoduodenectomy （LPD） in patients with pancreatic ductal adenocarcinoma （PDAC）， and to establish a predictive model. MethodsA retrospective analysis was performed for the clinical data of 240 PDAC patients who underwent LPD in The First Hospital of Jilin University from April 2016 to July 2022， with early postoperative tumor recurrence （time to recurrence ≤12 months） as the study outcome. The patients were randomly divided into training group with 168 patients and validation group with 72 patients at a ratio of 7∶3. In the training group， there were 70 patients （41.67%） with early postoperative recurrence and 98 （58.33%） without early recurrence， and in the validation group， there were 32 （44.44%） with early postoperative recurrence and 40 （55.56%） without early recurrence. The chi-square test or the Fisher’s exact test was used for comparison of categorical data between groups； a logistic regression analysis was used to investigate the risk factors for early postoperative recurrence； the receiver operating characteristic （ROC） curve and the area under the ROC curve （AUC） were used to evaluate the discriminatory ability of the model， with AUC>0.75 indicating that the model had adequate discriminatory ability. The Bootstrap resampling method was used for validation after 1 000 times of random sampling， and the model was validated again in the validation group. The calibration curve and the Hosmer-Lemeshow goodness-of-fit test were used to evaluate the degree of calibration， and the decision curve analysis was used to evaluate clinical practicability. ResultsThe univariate and multivariate analyses showed that preoperative CA19-9 level≥37 U/mL （odds ratio ［OR］=6.265， 95% confidence interval ［CI］： 1.938‍ ‍—‍ ‍20.249， P<0.05）， maximum tumor diameter >3 cm （OR=10.878， 95%CI： 4.090‍ ‍—‍ ‍28.932， P<0.05）， poor tumor differentiation （OR=3.679， 95%CI： 1.435‍ ‍—‍ ‍9.433， P<0.05）， lymph node metastasis （OR=0.209， 95%CI： 0.080‍ ‍—‍ ‍0.551， P<0.05）， and absence of adjuvant chemotherapy after surgery （OR=0.167， 95%CI： 0.058‍ ‍—‍ ‍0.480， P<0.05）. A nomogram model was constructed based on these factors； the ROC curve analysis showed that the model had an AUC of 0.895 （95%CI： 0.846‍ ‍—‍ ‍0.943， P<0.001）， and the calibration curve and the Hosmer-Lemeshow test showed that the model had a good degree of calibration （P=0.173）. The decision curve analysis showed that the nomogram had a good clinical application value. ConclusionPreoperative CA19-9 level ≥37 U/mL， maximum tumor diameter >3 cm， poor tumor differentiation， lymph node metastasis， and absence of adjuvant chemotherapy after surgery are independent risk factors for the early recurrence of PDAC after LPD， and the nomogram model established based on these factors can effectively predict early postoperative recurrence.

Objective To explore the experience of patients with rheumatoid arthritis participating in the shared medical appointments(SMA),so as to provide references for medical staff to formulate targeted intervention strategies.Methods By the descriptive phenomenology research method in qualitative research,14 patients with rheumatoid arthritis who participated in the SMA in a tertiary A integrated traditional Chinese and western medicine hospital in Shanghai from August to October 2023 were selected for the in-depth interviews,and the data were analyzed and themes were extracted by Colaizzi 7-step analysis method.Results The experience of patients with rheumatoid arthritis participating in SMA can be summarized into 3 themes:①Driving factors of participation in SMA(seeking clarity and resolution,seeking support and hope);②Perceived benefits of participation in SMA(feelings of efficiency and convenience,reduced isolation,increased security,reduced stigma,increased self-efficacy,insights and experiences,lifestyle changes);(3)Barriers to participation in shared outpatient procedures(obscure medical terminology,inapplicability to some special groups,single content and form of sharing,worries and concerns about personal privacy disclosure,transportation inconvenience and time conflicts).Conclusion Medical staff should pay attention to the experience of patients with rheumatoid arthritis participating in the SMA,improve the training skills of medical staff,solve the obstacles in the process of participation in the SMA,and promote the continuous improvement and development of the SMA.

Objective Based on the original text of Synopsis of Golden Chamber,we summarized and analyzed the pulse manifestation,characteristics of disease location,nature and syndrome,and main diseases of the stringed pulse in order to provide a systematic reference for its clinical application.Methods The sentences containing"stringed pulse"were sorted out according to the order of chapters in Synopsis of Golden Chamber firstly.Then the characteristics of pulse manifestation and pathogenesis were summarized.Finally,its main diseases and clinical significance were analyzed.Results This study summarized characteristics of the stringed pulse manifestation in Synopsis of Golden Chamber:the pulse flexibility is as tight as a bow string,the pulse shapes are straight up and down.And characteristics of the string pulse pathogenesis:the disease location is mainly interior,located in the chest,abdomen,liver and ribs;the nature of disease mainly tends to cold syndrome and excess syndrome;the main symptom is pain.There are seven main diseases related to string pulse:disease with symptoms such as convulsion,opisthotonos,trismus;malaria;stagnation of qi in chest;cold abdominal colic;Fluid retention disease;gastric disorder causing nausea and spasm.Conclusion The characteristics and main diseases of the string pulse reflected in the Synopsis of the Golden Chamber embody the thinking mode of diagnosis and treatment based on disease,syndrome and pulse,which has had a profound impact on the theory of diagnosis and treatment in later generations.