Objective To investigate the effects of Andai therapy based on metabolomics on people with different constitution of Mongolian Medicine.Methods On the basis of literature research,expert interviews,questionnaires and field investigation on the historical origin,operational methods,clinical effects,indications and contraindications of Andai therapy,non-targeted metabolomics detection was performed on baseline plasma of all included cases by gas chromatography mass spectrometry(GC-MS).The differences in metabolites between the experimental group(three body groups receiving Andai therapy)and the control group(three body groups not receiving Andai therapy)were compared,and functional analysis of related metabolites was conducted,and the factors related to regulatory mechanisms were explored by screening related metabolites and their pathways.Results Andai therapy can significantly reduce the content of harmful metabolites in the experimental group,indicating that it may regulate the level of metabolites in vivo.The response of different constitutions to Andai therapy was different,with statistical significance(P＜0.05).The contents of metabolites such as serotonin and abietic acid in the samples of Heyi constitutions were reduced,while the contents of Xila constitutions and Baganda constitutions showed more complex changes.The effects of Andai therapy on metabolic pathways are mainly reflected in the citric acid cycle,glutathione metabolism and lipid metabolism,which may promote energy metabolism and other physiological processes.Conclusion Andai therapy shows potential in regulating metabolite levels and metabolic pathway activity in vivo,but further studies are needed to fully evaluate the feasibility and effectiveness of its clinical application.

Objective To investigate the effects of Andai therapy based on metabolomics on people with different constitution of Mongolian Medicine.Methods On the basis of literature research,expert interviews,questionnaires and field investigation on the historical origin,operational methods,clinical effects,indications and contraindications of Andai therapy,non-targeted metabolomics detection was performed on baseline plasma of all included cases by gas chromatography mass spectrometry(GC-MS).The differences in metabolites between the experimental group(three body groups receiving Andai therapy)and the control group(three body groups not receiving Andai therapy)were compared,and functional analysis of related metabolites was conducted,and the factors related to regulatory mechanisms were explored by screening related metabolites and their pathways.Results Andai therapy can significantly reduce the content of harmful metabolites in the experimental group,indicating that it may regulate the level of metabolites in vivo.The response of different constitutions to Andai therapy was different,with statistical significance(P＜0.05).The contents of metabolites such as serotonin and abietic acid in the samples of Heyi constitutions were reduced,while the contents of Xila constitutions and Baganda constitutions showed more complex changes.The effects of Andai therapy on metabolic pathways are mainly reflected in the citric acid cycle,glutathione metabolism and lipid metabolism,which may promote energy metabolism and other physiological processes.Conclusion Andai therapy shows potential in regulating metabolite levels and metabolic pathway activity in vivo,but further studies are needed to fully evaluate the feasibility and effectiveness of its clinical application.