Objective:To evaluate the diagnostic value of intestinal tissue metagenomic next-generation sequencing (mNGS) in severe diarrhea following haploidentical allogeneic hematopoietic stem cell transplantation (allo-HSCT) .Methods:Sixteen patients who developed severe diarrhea or hematochezia after haploidentical allo-HSCT at the First Affiliated Hospital of Fujian Medical University (June 2023–August 2024) were enrolled. All underwent gastrointestinal endoscopy and mNGS for microbial detection. Clinical, endoscopic, pathological, and microbiological data were analyzed to evaluate the diagnostic value of mNGS and treatment outcomes following targeted therapy.Results:The study included 16 patients (12 males, 4 females; median age 32.5 years, range 3–60 years). Diarrhea occurred a median of 3.93 months post-transplant (range 1.63–10.40 months). Stool cultures were negative except for one case with Candida. One patient tested positive for Clostridium difficile antigen. Endoscopy revealed mucosal congestion, edema, erosion, and bleeding, with focal inflammation on pathology. mNGS detected pathogens in 87.5% (14/16) of cases, including mixed infections in 78.5% (11/14). Common pathogens were Klebsiella pneumoniae, Enterococcus faecium, Escherichia coli, Rhizopus microsporus, EBV, and CMV. Targeted treatment adjustments led to symptom improvement in 87.5% of patients.Conclusion:Allo-HSCT patients are prone to infectious diarrhea due to immunosuppression. Molecular analysis of endoscopic biopsy tissues using mNGS can accurately identify pathogens, guide targeted therapy, and improve clinical outcomes.

Objective:To evaluate the diagnostic value of intestinal tissue metagenomic next-generation sequencing (mNGS) in severe diarrhea following haploidentical allogeneic hematopoietic stem cell transplantation (allo-HSCT) .Methods:Sixteen patients who developed severe diarrhea or hematochezia after haploidentical allo-HSCT at the First Affiliated Hospital of Fujian Medical University (June 2023–August 2024) were enrolled. All underwent gastrointestinal endoscopy and mNGS for microbial detection. Clinical, endoscopic, pathological, and microbiological data were analyzed to evaluate the diagnostic value of mNGS and treatment outcomes following targeted therapy.Results:The study included 16 patients (12 males, 4 females; median age 32.5 years, range 3–60 years). Diarrhea occurred a median of 3.93 months post-transplant (range 1.63–10.40 months). Stool cultures were negative except for one case with Candida. One patient tested positive for Clostridium difficile antigen. Endoscopy revealed mucosal congestion, edema, erosion, and bleeding, with focal inflammation on pathology. mNGS detected pathogens in 87.5% (14/16) of cases, including mixed infections in 78.5% (11/14). Common pathogens were Klebsiella pneumoniae, Enterococcus faecium, Escherichia coli, Rhizopus microsporus, EBV, and CMV. Targeted treatment adjustments led to symptom improvement in 87.5% of patients.Conclusion:Allo-HSCT patients are prone to infectious diarrhea due to immunosuppression. Molecular analysis of endoscopic biopsy tissues using mNGS can accurately identify pathogens, guide targeted therapy, and improve clinical outcomes.

Objective To investigate clinical efficacy and safety of misoprostol for termination with full-term pregnancy.Methods The number who had terminations with full-term pregnancy in our hospital obstetrics from July 2013 to March 2014 were 78, divided them into the experimental and control groups.Experimental group used misoprostol for induction of labor, the control group used oxytocin for induction of labor.Indicators were observed and compared between two groups of pregnant women, induction of labor effect indicators: the success rate of induction of labor, postpartum hemorrhage in 2h, the time of labor and the time of fetal delivery, cesarean section rate; induction of labor safety indicators: maternal complications and side effects, the physiological condition of the newborns and Apgar score.Results Basic information was no significant different between the two groups of pregnant women.induction of labor of experimental group success rate was 97.44%, which was 82.05% in the control group, success rate in experimental group was higher than the control group, with statistically significant results (P<0.05), postpartum hemorrhage in 2h, labor time, fetal delivery time, cesarean section in experimental groups were less than which in the control group, the difference was statistically significant (P<0.05).Experimental group of pregnant women postpartum complications and side effects were mainly in the gastrointestinal adverse reactions, uterine contractions too strong but compared with the control group showed no significant difference.Two newborn babies 1 minute (9.4 ±1.3 vs.9.2 ±1.5) and 5 minute (9.9 ±1.2 vs.9.5 ±1.3) Apgar score were not significantly different (P>0.05), the experimental group had one case of mild neonatal asphyxia, there are two cases in the control group and one case of mild asphyxia neonatal asphyxia.Conclusion Effect of misoprostol which is used to terminate the pregnancy term is significant, and has high security, has high clinical value.