Objective:To evaluate the effect of sleep deprivation on the expression of sirtuin 6 (SIRT6) in the cerebellum of immature mice.Methods:Fifty SPF healthy male C57BL/6 mice, aged 4 weeks, weighing 14-16 g, were divided into 2 groups ( n=25 each) using a random number table method: control group (Con group) and sleep deprivation group (SD group). The chronic sleep deprivation model was prepared by using the multi-platform water environment method, with 20 h of sleep deprivation per day for 10 consecutive days. After sleep deprivation, a balance beam experiment was performed to test the balance and coordination ability of mice. The mice were sacrificed after anesthesia and cerebellar lobular IV-VI (4-6 cb) tissues were taken for microscopic examination of the ultrastructure (with a transmission electron microscope) and for determination of the dendritic spine density of cerebellar 4-6cb Purkinje neurons (by Golgi staining), co-expression of SIRT6 and Calbindin D-28k (CbD-28k) and expression of glucose transporter Glut3 of cerebellar 4-6cb (by immunofluorescence staining). Results:Compared with group Con, the duration of passage through the balance beam was significantly prolonged, and the number of posterior foot slips was increased, the synaptic gap of cerebellar 4-6cb neurons was increased, the thickness of postsynaptic density was increased, the density of dendritic spines of Purkinje cells and the number of positive cells co-expressing SIRT6 and CbD-28k were decreased, and the expression of Glut3 was down-regulated in group SD ( P<0.05). Conclusions:The mechanism by which sleep deprivation decreases the abilities of balance and coordination is related to down-regulating SIRT6 expression in cerebellar Purkinje cells and decreasing neuronal glucose metabolism, thus damaging the synaptic plasticity of cerebellum in immature mice.

Objective: To explore the emotional and behavioral problems of different only child and multiple child families, and to provide a reference for developing effective parenting styles. Methods: A stratified random cluster sampling method was adopted to investigate 2 647 guardians of preschool children in Tongling City from April to June, 2022. Children s emotional and behavioral problems were evaluated by using the self designed parenting questionnaire and the Children s Strengths and Difficulties Questionnaire (SDQ) parent edition. Results: The abnormal total score detection rate of emotional and behavioral problems of preschool children was 15.5%, and the rate of abnormal peer interaction was the highest (19.5%). In multiple child families, the first born child (17.5%, 20.4%), compared with the second and third child (11.5%, 9.5%), was more likely to exhibit conduct and hyperactivity problems ( χ 2=8.44, 29.75, P <0.01). There were differences in parenting attitudes between only child and multiple child families( χ 2=9.38, P <0.05). The results of the Logistic regression analysis showed that more frequent parent child discussion, the persuasive education and consistent family discipline strategy were negatively related to the emotional and behavioral problems of only children and children in multiple child families ( OR =0.15, 0.49, 0.38; 0.34, 0.40, 0.42, P <0.05). However, harmonious family relationships were only negatively related to emotional and behavioral problems in only children ( OR =0.08, P <0.01), and a higher education level among mothers was negatively related to emotional and behavioral problems in children from multiple child family environments( OR=0.30, 0.45, P <0.05). Conclusion The emotional and behavioral problems are serious of preschool children in Tongling City, the psychological development of the oldest children from multiple child and only child families should be actively followed, as this would help to promote a better understanding of the development of preschool children s physical and mental health.

The spread of COVID-19 has greatly threatened human health and economic growth. Angiotensin-converting enzyme 2 (ACE2) is a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-COV-2). By attaching to ACE2, SARS-COV-2 reduces its expression and induces lung injury. Vitamin D can inhibit the progression of COVID-19 by inhibiting the activity of ROCK pathway, up-regulating ACE2 expression and bio-availability, and slowing down the adverse reactions caused by Ang II accumulation. This study explored a novel mechanism, i.e., vitamin D protects against COVID-19-induced injury by upregulating ACE2 expression. It provides theoretical guidance for the role of Vitamin D in the prevention and treatment of COVID-19.

Objective:To evaluate the role of sonic hedgehog (Shh)/glioma-associated oncogene homolog 1 (Gli1) signaling pathway in sleep deprivation-induced cognitive impairment in young mice.Methods:Forty-eight SPF healthy male C57BL/6 mice, aged 4 weeks, weighing 14-16 g, were divided into 3 groups ( n=16 each) by the random number table method: control group (C group), sleep deprivation group (SD group) and Shh agonist SAG group (SD+ SAG group). Multi-platform water environment method was used to prepare the sleep deprivation model in mice, and the sleep deprivation was 20 h a day for 10 consecutive days.In SD+ SAG group, SAG 10 mg/kg was intraperitoneally injected at 5 min before each sleep deprivation, while the equal volume of normal saline was intraperitoneally injected in group C and group SD.The mice underwent novel object recognition and Y-maze tests at 24 h after development of the model.Mice were sacrificed after the behavioral testing, and the hippocampi were isolated for determination of the density of dendritic spines in hippocampal CA1 region (by Golgi staining), expression of Gli1 and brain-derived neurotrophic factor (BDNF) in hippocampal tissues (by Western blot), and expression of Gli1 and BDNF mRNA in hippocampal tissues (by quantitative real-time polymerase chain reaction). Results:Compared with group C, the preference index in novel object recognition and Y-maze tests and density of dendritic spines in CA1 region were significantly decreased, and the expression of Gli1 and BDNF protein and mRNA in hippocampus was down-regulated in group SD ( P<0.05). Compared with group SD, the preference index in novel object recognition and Y-maze tests and density of dendritic spines in CA1 region were significantly increased, and the expression of Gli1 and BDNF protein and mRNA in hippocampus was up-regulated in group SD+ SAG ( P<0.05). Conclusions:Inhibition of Shh/Gli1 signaling pathway and reduction of plasticity of dendritic spines of hippocampal neurons are involved in sleep deprivation-induced cognitive impairment in young mice.

Objective:To study our initial experience on feasibility and safety of magnetic compression anastomosis in laparoscopic pancreaticoduodenectomy(LPD).Methods:A retrospective analysis was conducted on the data of 7 patients who underwent LPD with laparoscopic magnetic compression choledochojejunostomy (LMC-CJ) or pancreaticojejunostomy (LMC-PJ) at the Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi’an Jiaotong University from May 2018 to September 2019. There were 6 males and 1 female. The median age of patients was 63 (56-83) years. Data analyzed included the model of the magnetic anastomosis device, operation time of the LMC-CJ or LMC-PJ, other operation-related parameters, postoperative complications, time to perform magnetic anastomosis, and time of discharge of the magnet from patients’ body.Results:All 7 patients completed LPD successfully, including 7 LMC-CJ and 2 LMC-PJ. The median operation time was 340 (310-450) minutes. The median diameter of the biliary-enteric magnetic anastomosis ring used was 10 (9-12) mm, and the median time of the biliary-enteric magnetic anastomosis was 11 (8-16) min. The diameter of the pancreaticojejunal magnetic anastomosis ring was 5 mm in the two anastomoses, and the times taken were 12 min and 15 min. Complications occurred in 4 patients, including 1 patient each for grade A and grade B pancreatic fistula, 2 patients with abdominal infection, 2 patients with postoperative gastric emptying disorder, and 1 patient with abdominal hemorrhage. All patients responded to conservative treatment. There was no biliary or pancreatic fistula at the magnetic anastomoses. Pancreaticojejunostomy functioned at 24 and 30 days after operation. The median time for the magnets to pass out from the body of all patients was 50 (40-170) days. The median follow-up was 11 (4-18) months. No biliary-enteric or pancreaticojejunostomy stenosis was detected.Conclusion:Magnetic compressive anastomosis was simple, feasible, and safe for choledochojejunostomy or pancreaticojejunostomy in LPD.

Surgical treatment for bone and soft tumors of pelvis and sacrum presents a big challenge, because of the complex anatomy of sacropelvic region, large tumor volume at presentation, rich blood supply to the tumor and visceral involvemen, et al. Therefore, surgical excision and reconstruction are technically difficult for sacropelvic tumors. Extensive intraoperative haemorrhage could be life-threatening, and this issue remains a major concern. How to effectively control bleeding during surgery is critical for successful operation and patient's favorable prognosis. Some previous attempts, such as interventional selective internal iliac artery embolization or manual ligation through an additional anterior approach, were tested to be ineffective. Inspired by the success of resuscitative endovascular balloon occlusion of the aorta (REBOA) which resemble an endovascular tourniquet for traumatic hemorrhagic shock, some researchers have applied this techinique to control surgical bleeding during pelvic or sacral tumor resection.The authors have performed REBOA for more than 1 500 sacropelvic tumr surgeries since 2003 in Peking University People's Hospital. The patient age, the diameter of femoral artery and aorta, atherosclerosis, as well as tumor location, volume and expansion and blood suppy, have to be thoroughly evaluated prior to REBOA administration. Admittedly, the application of REBOA do reduce intraoperative bleeding, shorten the operation duration, improve the safety of surgery, yet some complications were observed including local hematoma at the puncture site, acute arterial thrombosis, femoral artery pseudoaneurysm or occlusio, et al. The purpose of this study is to review the literature on REBOA administration in pelvic and sacral tumors excision, with the focus on its indications, performing procedure, the safety and efficacy, and complications. Moreover, in order to popularize the clinical application of aortic balloon occlusion in the future, we summarize our experience of abdominal aortic balloon occlusion over 10 years.

[摘 要] 目的：探讨miR-17-5p在胃肠道间质瘤（gastrointestinal stromal tumor，GIST）组织中的表达及其对GIST882细胞增殖与凋亡的影响。方法：选取2019年5月至2020年5月广西医科大学第四附属医院胃肠外科手术切除的20例GIST患者的瘤组织及相应的瘤旁组织标本，以及GIST882细胞和人正常肠道上皮细胞HIEC为研究对象。荧光PCR-毛细管电泳测序法检测GIST标本中KIT基因突变情况。分别将miR-17-5p mimic和pc-KIT转染至GIST882细胞中。双荧光素酶报告基因实验验证miR-17-5p与KIT的靶向关系。qPCR和WB法检测GIST组织和细胞中miR-17-5p、KIT mRNA及蛋白的表达，CCK-8法、流式细胞术检测GIST882细胞的增殖、凋亡及细胞周期进程。结果：20例GIST组织中有15例患者发生KIT基因突变。与瘤旁组织比较，GIST组织中miR-17-5p表达水平显著降低、KIT mRNA表达水平显著升高（均P<0.01）；与HIEC细胞比较，GIST882细胞中miR-17-5p表达显著降低、KIT mRNA和蛋白表达显著升高（均P<0.01）。过表达miR-17-5p可显著降低GIST882细胞的增殖能力（P<0.01）、提高细胞凋亡率（P<0.05）、sub-G1期和S期细胞比例显著增加（均P<0.05）、而G0/G1期的细胞比例显著减少（P<0.05），同时KIT蛋白表达水平明显降低（P<0.01）。双荧光素酶报告基因实验证实KIT是miR-17-5p的下游靶基因。同时过表达miR-17-5p和KIT对GIST882细胞的增殖、细胞周期进程和凋亡水平未产生明显影响。结论：过表达miR-17-5p可显著抑制GIST882细胞的增殖并诱导细胞凋亡，同时下调KIT蛋白的表达，miR-17-5p可能是治疗GIST的潜在靶标。

Objective:To investigate the causes,prevention and treatment of femoral artery puncture related complications caused by the application of resuscitative endovascular balloon occlusion of the aorta (REBOA) in the resection of pelvic and sacral tumors.Methods:Clinical data of 23 patients with femoral artery puncture related complications who received REBOA in the resection of pelvic and sacral tumors from August 2010 to August 2018 at the Musculoskeletal Tumor Center,Peking University People′s Hospital were retrospectively analyzed.There were 8 males and 15 females,with the age of (37.0±16.2) years (range:15 to 65 years).Arterial access via the Seldinger technique for REBOA was obtained in the right common femoral artery of 18 cases,and in the left of 6 cases.An arterial sheath with a diameter of 11 to 12 F(1 F≈0.33 mm) was used for the patient.The occurrence and treatment of postoperative complications were analyzed.Results:Acute femoral arterial thrombosis occurred in 18 patients,which was managed by open repair 48 hours postoperatively.Among the 349 patients admitted before 2015 who received hemostasis by compression after femoral artery sheath removal,12 patients (3.4%) developed acute femoral artery thrombosis.While the 476 patients admitted after 2015 who used a percutaneous suture device to close the femoral artery wound,6 patients (1.3%) developed acute femoral artery thrombosis.One case of retroperitoneal hematoma and 1 case of femoral pseudoaneurysm were found and surgically fixed.Postoperative follow-up was (40±18) months (range:13 to 108 months).Three cases with chronic lower extremity ischemia were confirmed by Doppler ultrasonography during 1 to 5 years follow-up.Two of them had minimal symptoms and denied further treatment,while the other one received femoral-femoral artery bypass surgery to restore distal flow for pain and numbness relief.Conclusions:Acute femoral arterial thrombosis is the most common femoral artery puncture.Technique refinement of REBOA,the use of percutaneous suture device and close follow-up can reduce the approach-specific complications,and help to detect and treat the complications timely,which may popularize the clinical application of REBOA.

Objective:To establish a method for the determination of sulfur dioxide (SO 2) in the air of workplace, which including the process of collection by absorption in triethanolamine aqueous solution and the process of analysis and detection by iron chromatography directly without derivatization. Methods:SO 2 in the air of workplace was collected at a flow rate of 0.5 L/min for 15 min through 10 ml of 3% triethanolamine aqueous solution filled in a porous glass plate absorption tube. The absorption solution passed through IonPacAS14A analytical column (4 mm×250 mm) and IonPacAG14 protective column (4 mm×50 mm) at a column temperature of 30 ℃, using Na 2CO 3 (3.0 mmol/L) -NaHCO 3 (1.0 mmol/L) as mobile phase with the flow rate of 1.2 ml/min, and was detected by conductivity detector. Results:It showed that SO 2 in triethanolamine solution could be stored for at least 7 d at room temperature (25 ℃) and 37 ℃. There was a linear relationship within the determination range of 0-8.00 μg/ml, the linear correlation coefficient was 0.9999. The sampling absorption efficiency was 98.1%-100.0%, the detection limit of the method was 0.018 μg/ml, the minimum detection concentration was 0.024 mg/m 3 (based on V0=7.5 L) . The recovery rate was 99.22%-101.69%, the intra batch precision was 0.41%-1.34%, and the inter batch precision was 0.73%-1.77%. Conclusion:The method has the advantages of simple operation, high sensitivity and good accuracy. It can prevent SO 2 from being oxidized and realize the direct detection of SO 2 in the air of workplace without derivation.

Objective:To establish a method for the determination of sulfur dioxide (SO 2) in the air of workplace, which including the process of collection by absorption in triethanolamine aqueous solution and the process of analysis and detection by iron chromatography directly without derivatization. Methods:SO 2 in the air of workplace was collected at a flow rate of 0.5 L/min for 15 min through 10 ml of 3% triethanolamine aqueous solution filled in a porous glass plate absorption tube. The absorption solution passed through IonPacAS14A analytical column (4 mm×250 mm) and IonPacAG14 protective column (4 mm×50 mm) at a column temperature of 30 ℃, using Na 2CO 3 (3.0 mmol/L) -NaHCO 3 (1.0 mmol/L) as mobile phase with the flow rate of 1.2 ml/min, and was detected by conductivity detector. Results:It showed that SO 2 in triethanolamine solution could be stored for at least 7 d at room temperature (25 ℃) and 37 ℃. There was a linear relationship within the determination range of 0-8.00 μg/ml, the linear correlation coefficient was 0.9999. The sampling absorption efficiency was 98.1%-100.0%, the detection limit of the method was 0.018 μg/ml, the minimum detection concentration was 0.024 mg/m 3 (based on V0=7.5 L) . The recovery rate was 99.22%-101.69%, the intra batch precision was 0.41%-1.34%, and the inter batch precision was 0.73%-1.77%. Conclusion:The method has the advantages of simple operation, high sensitivity and good accuracy. It can prevent SO 2 from being oxidized and realize the direct detection of SO 2 in the air of workplace without derivation.