Objective To study the characteristics of intestinal flora between diarrhea-predominant irritable bowel syndrome(IBS-D)and healthy population,and to provide a clinical basis for the basic research of IBS-D.Methods A total of 32 IBS-D patients admitted to Wenzhou People's Hospital,the Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University from January 2023 to June 2024 were selected as IBS-D group,and 33 healthy during the same period as control group.Cluster diversity was determined by 16S rDNA of clusters in feces and quantification of DNA.Results The diversity of IBS-D group decreased significantly than control group,and the relative abundance of proteobacteria increased in the IBS-D group,and the relative abundance of E.coli-Shigella and Klebsiella increased in the IBS-D group.Conclusion Intestinal microbiota diversity in IBS-D patients reduced and proteobacteria is the potential pathogen of IBS-D.

Objective To study the characteristics of intestinal flora between diarrhea-predominant irritable bowel syndrome(IBS-D)and healthy population,and to provide a clinical basis for the basic research of IBS-D.Methods A total of 32 IBS-D patients admitted to Wenzhou People's Hospital,the Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University from January 2023 to June 2024 were selected as IBS-D group,and 33 healthy during the same period as control group.Cluster diversity was determined by 16S rDNA of clusters in feces and quantification of DNA.Results The diversity of IBS-D group decreased significantly than control group,and the relative abundance of proteobacteria increased in the IBS-D group,and the relative abundance of E.coli-Shigella and Klebsiella increased in the IBS-D group.Conclusion Intestinal microbiota diversity in IBS-D patients reduced and proteobacteria is the potential pathogen of IBS-D.