Objective:To study the application of hyperbaric oxygen (HBO) combined with out-of-hospital continuing nursing based on three-dimensional quality evaluation model in patients with ulcerative colitis, and investigate the factors influencing patients’ quality of life.Methods:All research objects were treated with HBO in Yan’an University Affiliated Hospital. The patients ( n=60) with ulcerative colitis treated from January 2018 to December 2018 were selected as the control group; while the patients ( n=60) treated from January 2019 to December 2019 were selected as the observation group. The control group received the conventional out-of-hospital continuing nursing, while the observation group received out-of-hospital continuing nursing based on three-dimensional quality evaluation model. The nursing time for both groups were three months. The negative emotions, quality of life, and self-care ability of the two groups were compared, and the risk factors affecting the quality of life were analyzed. Results:After nursing, the Zung self-rating anxiety scale (SAS) and Zung self-rating depression scale (SDS) scores of the two groups decreased significantly, and the observation group decreased more significantly than the control group in terms of the SAS scores (33.14±3.86 vs. 43.55±3.77) and the SDS scores (35.47±2.95 vs. 45.46±3.46), and the differences were statistically significant ( P<0.05). The physical functioning ( t=2.563, P=0.012), role physical ( t=3.539, P=0.001), bodily pain ( t=2.448, P=0.016), general health ( t=5.301, P<0.001), vitality ( t=2.489, P=0.014), social functioning ( t=3.860, P<0.001), role emotional ( t=5.883, P<0.001), mental health ( t=3.729, P<0.001), and other indicators of the observation group were significantly higher than those of the control group. After nursing, the assessment scores of drug dependence, psychological adjustment ability, sleep quality, diet adjustment, and pain recognition in the observation group were significantly higher than those in the control group; the differences in quality of life assessment involving gender, education level, monthly income, marital status, mental status, exercise, lifestyle, drinking, smoking, and comorbid chronic diseases between the two groups were significant ( P<0.05); through multivariate analysis, gender, education level, monthly income, marital status, mental status, exercise, lifestyle, drinking, smoking, and comorbid chronic diseases were independent risk factors affecting the quality of life of patients. Conclusion:HBO combined with out-of-hospital continuing nursing based on three-dimensional quality evaluation model is of positive influence on the negative emotions and quality of life of patients with ulcerative colitis. And it is suggested high attention be paid to unmarried patients, and the patients with low monthly income, severe negative emotions, low education level, and chronic diseases.

Objective:To study the application of hyperbaric oxygen (HBO) combined with out-of-hospital continuing nursing based on three-dimensional quality evaluation model in patients with ulcerative colitis, and investigate the factors influencing patients’ quality of life.Methods:All research objects were treated with HBO in Yan’an University Affiliated Hospital. The patients ( n=60) with ulcerative colitis treated from January 2018 to December 2018 were selected as the control group; while the patients ( n=60) treated from January 2019 to December 2019 were selected as the observation group. The control group received the conventional out-of-hospital continuing nursing, while the observation group received out-of-hospital continuing nursing based on three-dimensional quality evaluation model. The nursing time for both groups were three months. The negative emotions, quality of life, and self-care ability of the two groups were compared, and the risk factors affecting the quality of life were analyzed. Results:After nursing, the Zung self-rating anxiety scale (SAS) and Zung self-rating depression scale (SDS) scores of the two groups decreased significantly, and the observation group decreased more significantly than the control group in terms of the SAS scores (33.14±3.86 vs. 43.55±3.77) and the SDS scores (35.47±2.95 vs. 45.46±3.46), and the differences were statistically significant ( P<0.05). The physical functioning ( t=2.563, P=0.012), role physical ( t=3.539, P=0.001), bodily pain ( t=2.448, P=0.016), general health ( t=5.301, P<0.001), vitality ( t=2.489, P=0.014), social functioning ( t=3.860, P<0.001), role emotional ( t=5.883, P<0.001), mental health ( t=3.729, P<0.001), and other indicators of the observation group were significantly higher than those of the control group. After nursing, the assessment scores of drug dependence, psychological adjustment ability, sleep quality, diet adjustment, and pain recognition in the observation group were significantly higher than those in the control group; the differences in quality of life assessment involving gender, education level, monthly income, marital status, mental status, exercise, lifestyle, drinking, smoking, and comorbid chronic diseases between the two groups were significant ( P<0.05); through multivariate analysis, gender, education level, monthly income, marital status, mental status, exercise, lifestyle, drinking, smoking, and comorbid chronic diseases were independent risk factors affecting the quality of life of patients. Conclusion:HBO combined with out-of-hospital continuing nursing based on three-dimensional quality evaluation model is of positive influence on the negative emotions and quality of life of patients with ulcerative colitis. And it is suggested high attention be paid to unmarried patients, and the patients with low monthly income, severe negative emotions, low education level, and chronic diseases.

Objective :To evaluate therapeutic effect of allisartan isoproxil tablet on carotid atherosclerosis (CAS) le‐sion in patients with mild to moderate essential hypertension (EH).Methods :A total of 120 patients with mild to moderate EH complicated CAS treated in our hospital were enrolled .They were randomly divided into valsartan group (n＝60 ,received valsartan 80mg／d) and allisartan isoproxil group (n＝60 ,received allisartan isoproxil tablet 240mg／d) ,both groups received measure by carotid vascular ultrasound before treatment ,24 weeks and 48 weeks af‐ter treatment and its result were compared between two groups .Results :Compared with before treatment ,there were significant reductions in IMT ,size ,thickness and number of carotid atherosclerotic plaques on 24 weeks and 48 weeks after treatment in two groups ,and above indexes of 48 weeks were significantly lower than those of 24 weeks , P＜0.05 or ＜0.01. The decreased value of IMT in allisartan isoproxil group was significantly higher than that of valsartan group ,and there were no significant difference between two groups in time point of size ,thickness and number of carotid atherosclerotic plaques , P＞0.05 all.Conclusion :Therapeutic effect of two drugs on size ,thick‐ness and number of carotid atherosclerotic plaques are similar ;but therapeutic effect of allisartan isoproxil tablet on IMT is significantly better than that of valsartan .

Objective To explore the immunopathological mechanism for the imbalance between the positive signal mediated by inducible costimulator (ICOS) and the negative signal mediated by programmed death-1 (PD-1) in patients with myasthenia gravis (MG).Methods Eighty-two patients with MG,56 healthy controls (HC) and 20 non-MG (NMG) patients,collected in the First Affiliated Hospital of Suzhou University from February 2014 to December 2016,were chosen to participate in the study.The expression of ICOS and PD-1 on peripheral blood mononuclear cells was detected by immuno-fluorescence staining and flow cytometry.The levels of soluble programmed death-1 (sPD-1),soluble programmed death ligand 1 (sPD-L1),IL-4 and other cytokines were detected by enzyme-linked immunosorbent assay.Results (1) Flow cytometry analysis:The co-expression of PD-1,ICOS on CD4 + T cells from MG group (9.64％ (8.82％)) was higher than in HC (1.81％ (2.10％),Z =-7.389,P ＜0.05) and NMG group (2.86％ (1.49％),Z =-4.636,P ＜ 0.05).The expression of ICOS on CD4 + T cells,ICOS ligand (ICOSL) on CD14+ monocytes and CD19+ B cells were increased in MG group comparing with that of the control groups.The proportion of PD-1 + CD4 + T cells (MG group 16.82％ (10.66％),HC 9.34％ (9.18％),Z =-4.345,P＜0.05;NMG group 7.07％ (3.40％),Z=-4.594,P＜0.05) and PD-1 Ligand (PD-L1) + CD14+ monocytes was higher in MG patients.All of these were detected by flow cytometry.(2) ELISA analysis:Serum sPD-1 expression significantly increased in MG group compared with that in the control groups (MG group (1.87 ± 0.64) ng/ml,NMG group (1.49 ± 0.70) ng/ml,t =2.04,P ＜ 0.05;HC (1.05 ± 0.50)ng/ml,t =2.08,P ＜ 0.05),while for serum sPD-L1,there was no significant difference between MG and control groups.(3) Serum cytokines detection:The expression of IL-4 was increased in MG patients (MG group (61.88 ±5.15) pg/ml,HC (32.03 ±1.84) pg/ml,t=2.50,P＜0.05;NMG group (42.62± 3.31) pg/ml,t =2.34,P ＜0.05),and there was a negative correlation between the expression of sPD-1 and the concentration of IL-4.Conclusions The increased expression of PD-1 + ICOS + CD4 + T cells suggested the subset involved in the pathological progress of MG.sPD-1 might disturb the ligation of PD-1 on T cells and PD-L1 on antigen presenting cells,while the ligation of ICOS and ICOSL passed positive signal,leading to over activity of the subsets and the progression of disease.

Aim To prepare the monoclonal antibodies (mAbs) against human CD28 and to study its biological feature. Methods The hybridoma cell lines were obtained by fusing spleen cells of Blab/c mice that had been immunized with murine lymphoma cells transfected with full-length huaman CD28 cDNA to myeloma cells Sp2/0. Ascites were induced to produce the mAbs. The specificity and affinity of the mAb 18G8 was verified by CD28 competitive inhibitory test and FACS. Reactivities of mAb 18G8 to PBTC, U266, 8226, Jurkat and Daudi cell were studied by indirect immunofluorescence staining. mAb 18G8-inducing proliferation of peripheral blood T cells (PBTCs) was determined by [3H]thymidine incorporation test. Results Five hybridoma cell lines were obtained. mAb 18G8 secreted by one of the them, belong to mouse IgG2a. It recognized a epitope different from which recognized by the standard mAb(clone CD28.2). The Reactivitrates of the mAb 18G8 to PBTC, U266, 8266, Jurkat and Daudi cells were 70.2％ , 99.3％ , 98.6％ , 76.4％ and 1.9％ , respectively, similar with CD28.2. It was indicated that different antigen epitopes expressed on all above cells. mAb 18G8 could promote the PBTC proliferation in vitro(SI=7). It was indicated that The substitution of mAb 18G8 for B7-1 molecule could also mediate the costimulatory signals. Conclusion 18G8 is a specific and functional anti-CD28 mAb it may be of significant value in basic studies and clinical application.