Objective To investigate the correlation between serum levels of long non-coding RNA(Ln-cRNA)-prostate androgen regulated transcript 1(PART1),LncRNA-nucleolar ribonucleic acid host gene 14(SNHG14)and disease stage,cognitive impairment and motor function in patients with Parkinson's disease(PD).Methods A total of 100 PD patients(PD group)who admitted to the Department of Neurology in the hospital from January 2021 to December 2023 and 100 healthy subjects(control group)who underwent the physical examination during the same period of time were selected.According to Hoehn-Yahr staging,PD pa-tients were divided into early stage group(grade 1.0-2.5,20 cases),middle stage group(grade 3.0,48 ca-ses)and late stage group(grade 4.0-5.0,32 cases).According to the Montreal Cognitive Assessment(Mo-CA)score,the patients were divided into normal cognitive group(MoCA score≥26 points,33 cases),PD-mild cognitive impairment group(MoCA score 21-＜26 points,46 cases)and PD dementia group(MoCA score＜21 points,21 cases).According to the Unified Parkinson's Disease Rating Scale(UPDRS)-Ⅲ score,the pa-tients were divided into mild dyskinesia group(0-15 points,29 cases),moderate dyskinesia group(＞15-40 points,46 cases)and severe dyskinesia group(＞40-56 points,25 cases).Real-time fluorescence quantitative PCR was used to detect serum LncRNA-PART1 and LncRNA-SNHG14 levels.Spearman method was used to analyze the correlation between serum LncRNA-PART1,LncRNA-SNHG14 levels and Hoehn-Yahr staging,MoCA score and UPDRS-Ⅲ score in PD patients.Results The level of serum LncRNA-PART1 in PD group was lower than that in control group(P＜0.05),and the level of LncRNA-SNHG14 was higher than that in control group(P＜0.05).The serum levels of LncRNA-PART1 in the middle stage group and late stage groups were lower than those in the early stage group(P＜0.05),and the levels of LncRNA-SNHG14 were higher than those in the early stage group(P＜0.05).In addition,the serum level of LncRNA-PART1 in the late stage group was lower than that in the middle stage group(P＜0.05),and the level of LncRNA-SNHG14 was higher than that in the middle stage group(P＜0.05).The serum LncRNA-PART1 levels in the PD-mild cognitive impairment group and PD dementia group were lower than those in the normal cognitive group(P＜0.05),while the LncRNA-SNHG14 levels were higher than those in the normal cognitive group(P＜0.05).Additionally,the serum LncRNA-PART1 level in the PD dementia group was lower than that in the PD-mild cognitive impairment(P＜0.05),while the LncRNA-SNHG14 level was higher than that in the PD-mild cog-nitive impairment group(P＜0.05).The serum levels of LncRNA-PART1 in the moderate dyskinesia group and severe dyskinesia group were lower than those in the mild dyskinesia group(P＜0.05),and the levels ofLncRNA-SNHG14 were higher than that in the mild dyskinesia group(P＜0.05).In addition,the serum level of LncRNA-PART1 in the severe dyskinesia group was lower than that in the moderate dyskinesia group(P＜0.05),and the level of LncRNA-SNHG14 was higher than that in the moderate dyskinesia group(P＜0.05).Spearman method results showed that serum LncRNA-PART1 level was negatively correlated with Hoehn-Yahr staging and UPDRS-Ⅲ score in PD patients,and positively correlated with MoCA score(P＜0.05).The level of serum LncRNA-SNHG14 was positively correlated with Hoehn-Yahr staging and UPDRS-Ⅲ score in PD patients,and negatively correlated with MoCA score(P＜0.05).Conclusion The level of ser-um LncRNA-PART1 in PD patients is decreased,and the level of LncRNA-SNHG14 is increased,both of them are related to the disease stage,cognitive impairment and motor function of PD patients,which may be-come evaluation indicators for PD progression.

Objective To explore the potential categories and influencing factors of fatigue trajectories in stroke patients,to provide information of the nursing management of patients with post-stroke fatigue after subsequent stroke.Methods 265 stroke patients hospitalized in the rehabilitation department of a tertiary A general hospital in Lishui from January 2022 to June 2023 were conveniently selected as the research subjects.The general information questionnaire,the Fatigue Severity Scale,the Health Behaviour Scale for Stroke Patient,and the Pittsburgh Sleep Quality Index were employed.The degree of fatigue was evaluated at 1～2 weeks,1 month,3 months and 6 months after the onset of the disease.The latent category growth model was used to identify the potential categories of fatigue trajectory,and logistic regression was used to analyze the influencing factors of fatigue trajectory.Results A total of 232 stroke patients were included,among which 128(55.17％)had fatigue,and there were 4 trajectories of fatigue,including 44 cases(18.97％)in the"continuous fatigue group",13 cases(5.60％)in the"increased fatigue group",71 cases(30.60％)in the"fatigue relief group",104 cases(44.83％)in the"no fatigue group".Logistic regression analysis showed that age,education level,health behavior and sleep quality were the influencing factors of fatigue trajectory in stroke patients(P＜0.05).Conclusion The fatigue of stroke patients can be divided into 4 kinds of change trajectories,and there is group heterogeneity.Nursing staff should carry out targeted nursing interventions for patients according to different fatigue change trajectories.

Objective To investigate the relationship between serum pro-nerve growth factor(proNGF),silent information regulator factor 2-related enzyme 1(SIRT1)levels and cognitive dysfunction in Parkinson's disease(PD)patients.Methods A total of 112 PD patients admitted to the Department of Neu-rology in the hospital from January 2022 to January 2023(PD group)were selected,and another 70 healthy volunteers for physical examination during the same period in the hospital(control group)were selected.The PD patients were divided into the PD dementia(PDD)group(24 cases),the PD-mild cognitive impairment(PD-MCI)group(51 cases),and the normal cognition group(37 cases)according to the cognitive function.Serum proNGF and SIRT1 levels were measured by enzyme linked immunosorbent assay.Multivariate Logis-tic regression was used to analyze the factors influencing the cognitive dysfunction in PD patients,and receiver operating characteristic curve was used to analyze the predictive value of serum proNGF and SIRT1 levels on cognitive dysfunction in PD patients.Results Compared with the control group,serum proNGF level was in-creased and SIRT1 level was decreased in the PD group(P＜0.05).Serum proNGF levels were sequentially increased and SIRT1 levels were sequentially decreased in the normal cognition group,PD-MCI group,and PDD group(P＜0.05).Prolonged disease duration,increased Hoehn-Yahr classification,increased unified Par-kinson's disease rating scale-Ⅲ score,and elevated proNGF level were independent risk factors for cognitive dysfunction in patients with PD(P＜0.05),and elevated SIRT1 was an independent protective factor(P＜0.05).The area under the curve of the combined prediction of serum proNGF and SIRT1 for cognitive dys-function in PD patients levels was 0.884,which was greater than 0.787 and 0.779 predicted by serum proNGF and SIRT1 alone(P＜0.05).Conclusion Elevated serum proNGF levels and reduced SIRT1 levels in PD pa-tients are closely associated with cognitive dysfunction,and the combination of serum proNGF and S1RT1 lev-els has a high predictive value for cognitive dysfunction in PD patients.

Objective:To understand the spectrum of pathogens and epidemic characteristics of respiratory infectious diseases in influenza virus-negative influenza-like cases in Yantai, and provide reference for disease prevention and control and clinical diagnosis and treatment.Methods:From March 2020 to February 2021, nasopharyngeal swab samples of 233 influenza virus-negative influenza like cases were collected in all sentinel hospitals monitored by Yantai National Influenza Network Laboratory, and 22 respiratory pathogens were detected by multiple fluorescence quantitative polymerase chain reaction to analyze epidemiological characteristics.Results:The total pathogen detection rate of 233 samples was 69.96% (163/233). A total of 17 respiratory pathogens were detected. The top three pathogens were human coronavirus (HCoV, 32.62%), rhinovirus/enterovirus (RhV/EV, 17.17%) and Legionella pneumophila (LP, 16.74%). The detection rates in different age groups were 80.28% (57/71) in the 0-15 years old group, 62.65% (52/83) in the 16-30 years old group, 68.18% (30/44) in the 31-45 years old group, 64.28% (9/14) in the 46-60 years old group, and 71.43% (15/21) in the >60 years old group. There was no significant difference among the groups. Respiratory pathogens were detected throughout the year, mainly in a single pathogen carrying mode (44.21%), and there was no significant difference in the physical examination rate of respiratory pathogens in different seasons. The seasonal prevalence of various pathogens was different, and the detection rate of HCoV 229E was the highest in spring (68.75%); the detection rate of rhinovirus/enterovirus was higher in autumn (26.98%) and winter (23.08%); the detection rate of LP was high in spring (19.05%) and summer (27.27%); the detection rate of human parainfluenza virus (HPIV) in spring (22.22%) was significantly higher than that in summer (3.64%). The number of HPIV and Bordetella pertussis (Bp) detected in the 0-15 year old group was the highest, and the detection rate was statistically significant among different age groups. Conclusions:The continuous monitoring of respiratory pathogens such as HCoV, RhV, EV, LP, HPIV should be strengthened to understand their epidemiologic characteristics and the standardization of pathogenicity, which provides data support and reference for epidemiological investigation of outbreaks that may be caused by other pathogens.

OBJECTIVETo study the damage of DNA in rat bone marrow cells induced by mustard gas.

METHODMale SD rats were randomly divided into six groups. Physiological saline, propylene glycol and mustard gas(0.2, 0.4, 0.8, 1.6 mg/kg) were given separately by i.p. injection. 5 rats in each group were killed after 0, 24, 48, 72 hours of exposure. The DNA damage in rat bone marrow cells was assayed by single cell gel electrophoresis (SCGE).

RESULTSThere is no significant difference of DNA damage among all groups at 0 h(P > 0.05). The rates of DNA migration and the lengths of DNA migration of the rat bone marrow cells in propylene glycol group at 24, 48, 72 hours were 15.4% +/- 0.21%, 16.0% +/- 0.19%, 15.7% +/- 0.23% and (11.4 +/- 0.2), (13.5 +/- 0.3), (12.8 +/- 0.2) micron respectively, and they were significantly higher than those of physiological saline group at the same time(P < 0.05). The rates of DNA migration and the lengths of DNA migration of the rat bone marrow cells in mustard gas groups at 24, 48, 72 hours were significantly higher than those in physiological saline group and propylene glycol group at the same time(P < 0.05).

CONCLUSIONMustard gas could induce DNA damage in rat bone marrow cells. The damage was likely to rise as the dose increased and was time-dependent.

Animals ; Bone Marrow Cells ; drug effects ; ultrastructure ; Comet Assay ; DNA Damage ; Dose-Response Relationship, Drug ; Male ; Mustard Gas ; toxicity ; Rats ; Rats, Sprague-Dawley ; Time Factors