Objective To analyze chemical constituents of compound Maxing Shigan decoction by ultra-high perfor-mance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). Methods The separation was performed on a UPLC BEH C₁₈ column (2.1 mm×100 mm, 2.5 µm)，with a gradient elution applying 0.1% aqueous formic acid solution and 0.1% formic acid acetonitrile as a mobile phase. The column temperature was 40 °C. The flow rate was 0.4 ml/min and the analysis time was 15 min. Mass spectrometry (MS) data were collected in both positive and negative ESI ion modes. Results Through UPLC-QTOF/MS analysis and reference validation, a total of 59 chemical components in Maxing Shigan decoction were identified. Conclusion An ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method was established to identify the chemical components of Maxing Shigan decoction. This method is simple, efficient, sensitive and accurate, and provides a basis for the elucidation of the pharmacodynamic material basis and mechanism of Maxing Shigan decoction. It can provide data reference for the optimization of the compatibility of traditional Chinese medicine in the treatment of COVID-19.

Spine fracture and dislocation are common traumatic spinal conditions that often require surgical intervention due to compromised spinal stability. Surgical approaches include anterior, posterior, and combined anterior-posterior spinal procedures. According to the specific surgical requirements, patients may be placed in the prone position or repositioned between prone and supine positions during surgery. Intraoperative repositioning has become an essential step in patient positioning. However, during repositioning, patients with spinal fracture and dislocation are at increased risk for complications such as hemodynamic instability, nerve injury, and pressure injuries to the skin and soft tissue. Notably, due to the instability of the spinal cord, even minor manipulations can further exacerbate the damage, potentially leading to severe outcomes like paraplegia. Although the current clinical guidelines provide instructive recommendations for standard position, there remains no specific protocols for intraoperative repositioning in patients with spine fracture and dislocation. With a concern for the lack of clinical studies on positioning techniques, risk prevention, and operational norms for special patients, no applicable guidelines or standards are available. A consensus was required to provide clinical reference, meet the requirements of surgical treatment, and minimize the safety risks of patients caused by improper placement of positions. Professional Committee of Operating Room Nursing of Shaanxi Nursing Association organized experts in nursing management and operating room nursing from major hospitals across China to formulate Expert consensus on intraoperative repositioning for patients with spinal fracture and dislocation ( version 2025). The consensus provides 11 recommendations covering pre-repositioning preparation, intraoperative maneuvers, and post-repositioning observation, aiming to provide references for clinical standardization of the intraoperative repositioning process and protection of patients′ safety.

Metastasis is the leading cause of death from cutaneous melanoma. Identifying metastasis-related targets and developing corresponding therapeutic strategies are major areas of focus. While functional genomics strategies provide powerful tools for target discovery, investigations at the protein level can directly decode the bioactive epitopes on functional proteins. Aptamers present a promising avenue as they can explore membrane proteomes and have the potential to interfere with cell function. Herein, we developed a target and epitope discovery platform, termed functional aptamer evolution-enabled target identification (FAETI), by integrating affinity aptamer acquisition with phenotype screening and target protein identification. Utilizing the aptamer XH3C, which was screened for its migration-inhibitory function, we identified the Chondroitin Sulfate Proteoglycan 4 (CSPG4), as a potential target involved in melanoma migration. Further evidence demonstrated that XH3C induces cytoskeletal rearrangement by blocking the interaction between the bioactive epitope of CSPG4 and integrin α4. Taken together, our study demonstrates the robustness of aptamer-based molecular tools for target and epitope discovery. Additionally, XH3C is an affinity and functional molecule that selectively binds to a unique epitope on CSPG4, enabling the development of innovative therapeutic strategies.

Gene regulation relies on the precise binding of transcription factors (TFs) at regulatory elements, but simultaneously detecting hundreds of TFs on chromatin is challenging. We developed cFOOT-seq, a cytosine deaminase-based TF footprinting assay, for high-resolution, quantitative genome-wide assessment of TF binding in both open and closed chromatin regions, even with small cell numbers. By utilizing the dsDNA deaminase SsdAtox, cFOOT-seq converts accessible cytosines to uracil while preserving genomic integrity, making it compatible with techniques like ATAC-seq for sensitive and cost-effective detection of TF occupancy at the single-molecule and single-cell level. Our approach enables the delineation of TF footprints, quantification of occupancy, and examination of chromatin influences on TF binding. Notably, cFOOT-seq, combined with FootTrack analysis, enables de novo prediction of TF binding sites and tracking of TF occupancy dynamics. We demonstrate its application in capturing cell type-specific TFs, analyzing TF dynamics during reprogramming, and revealing TF dependencies on chromatin remodelers. Overall, cFOOT-seq represents a robust approach for investigating the genome-wide dynamics of TF occupancy and elucidating the cis-regulatory architecture underlying gene regulation.
Transcription Factors/genetics* ; Humans ; Chromatin/genetics* ; Animals ; Binding Sites ; Mice ; DNA Footprinting/methods*

Objective:To evaluate the clinical efficacy of the V-shaped bone tunnel technique at the base of the distal phalanx for treating tendinous mallet finger.Methods:A retrospective analysis was conducted on clinical data from patients with tendinous mallet finger treated in the Department of Hand and Foot Surgery at Linyi People’s Hospital between August 2022 and November 2023. Two oblique bone tunnels were created in a V-shaped configuration at the dorsal base of the distal phalanx, adjacent to the extensor tendon insertion, using a 0.8 mm Kirschner wire. A 4-0 double-needle monofilament tendon suture was passed through the tunnels to secure the ruptured extensor tendon to the base of the distal phalanx, followed by fixation of the distal interphalangeal (DIP) joint with a 1.0 mm Kirschner wire. The Kirschner wire was removed at 4 weeks postoperatively to initiate functional exercises. Regular follow-up was conducted to monitor wound healing and functional recovery of the DIP joint. At the final follow-up, the range of flexion and extension of the DIP joint was measured, and treatment outcomes were evaluated using Crawford’s mallet finger evaluation criteria, which classified results into four grades: excellent, good, fair, and poor.Results:Fifteen patients (16 fingers) were included, comprising 11 males and 4 females, with a mean age of 44.5 years (range: 17-65 years). The injured fingers included 2 index, 4 middle, 5 ring, and 5 little fingers, all presenting with DIP joint flexion deformity and limited active extension. Postoperative follow-up ranged from 6 to 28 months (mean: 17 months). All wounds healed primarily without complications such as infection or skin necrosis, and no cases of tendon re-rupture occurred. At the final follow-up, the measurement results of flexion and extension range of motion of the affected fingers at the DIP joint were as follows: the maximum flexion angle of all 16 fingers was 45°, among which 7 fingers had an extension angle of 0°, 8 fingers had limited extension ranging from 1° to 10°, and 1 finger had limited extension of 15°. Among the 16 fingers, 7 fingers were rated as excellent, 8 fingers as good and 1 finger as fair.Conclusion:The V-shaped bone tunnel technique for tendon-to-bone reattachment of the extensor tendon insertion is a simple and effective method for treating tendinous mallet finger. It provides satisfactory functional recovery, improves finger appearance, and is associated with minimal complications.

To explore the mediating effect of resilience between sleep quality index and quality of life in lung cancer patients,we used General data questionnaire,Pittsburgh Sleep Quality Index,Connor-Davidson Resilience Scale,Chinese version Memorial Symptom Assessment Scale and functional Assessment of Cancer Therapy-Lung to investigate 218 lung cancer patients in the Department of Respiratory and Critical Care Medicine,Zhongshan Hospital,Fudan University during Nov 2023 to Feb 2024.A structural equation model was constructed to analyze the mediating effect of resilience between sleep quality index and quality of life.The sleep quality of lung cancer patients was in mild sleep disturbance.There was a negative correlation between sleep quality index and quality of life.Resilience of the patients partly mediated the relationship between sleep quality index and quality of life with an effect of 0.826,accounting for 28.47%of the total effect.Doctors and nurses should pay attention to enhancing the resilience level of lung cancer patients and improving their impact of sleep disturbance on the quality of life.

Objective:To investigate the impact of regional innovation on medical security and its spatial effects across 31 provinces in China,providing scientific evidence for synergizing the"Healthy China"strategy with regional coordinated development.Methods:Based on provincial panel data from 2007 to 2023,the medical insurance fund expenditure was employed as a proxy for medical security.Methodologies include Moran's I tests,dual-way fixed effects models,Spatial Durbin Models(SDM)with adjacency and inverse-distance matrices,and spatial mediation models were applied to comprehensively analyze the direct effects,spatial spillover effects,and mediating pathways of regional innovation on medical security.Results:During the observation period,medical security levels in all provinces showed a fluctuating upward trend with significant regional disparities,where eastern provinces outperformed central and western regions.Regional innovation exhibited significant positive direct effects and spatial spillover effects on medical security.Regional innovation can improve medical insurance coverage by promoting growth in medical financial expenditure and the conversion of medical patents.Conclusion:Policy recommendations include deepening the synergy between regional innovation and medical security,optimizing the allocation of medical fiscal resources,accelerating the transformation of technological innovations,and narrowing regional development gaps.

Animal models of TCM syndromes are the important carriers of revealing the basic theory of traditional Chinese medicine(TCM)and promoting the clinical development of TCM,and are indispensable tools to promote the modernization and internationaliza-tion of TCM.At present,the establishment methods of TCM syndrome animal model are varied and the evaluation criteria are different.Based on the main physiological functions of the five viscera,this paper summarized and analyzed the construction and evalu-ation methods of the most commonly used animal models of TCM syndromes in recent years,represented by the common clinical heart-qi deficiency syndrome,liver stagnation syndrome,spleen-qi deficiency syndrome,lung-qi deficiency syndrome and kidney-yang de-ficiency syndrome,in order to provide reference for establishing a more scientific and objective animal model construction method and evaluation system of TCM syndromes.

To explore the mediating effect of resilience between sleep quality index and quality of life in lung cancer patients,we used General data questionnaire,Pittsburgh Sleep Quality Index,Connor-Davidson Resilience Scale,Chinese version Memorial Symptom Assessment Scale and functional Assessment of Cancer Therapy-Lung to investigate 218 lung cancer patients in the Department of Respiratory and Critical Care Medicine,Zhongshan Hospital,Fudan University during Nov 2023 to Feb 2024.A structural equation model was constructed to analyze the mediating effect of resilience between sleep quality index and quality of life.The sleep quality of lung cancer patients was in mild sleep disturbance.There was a negative correlation between sleep quality index and quality of life.Resilience of the patients partly mediated the relationship between sleep quality index and quality of life with an effect of 0.826,accounting for 28.47%of the total effect.Doctors and nurses should pay attention to enhancing the resilience level of lung cancer patients and improving their impact of sleep disturbance on the quality of life.

Objective:To investigate the impact of regional innovation on medical security and its spatial effects across 31 provinces in China,providing scientific evidence for synergizing the"Healthy China"strategy with regional coordinated development.Methods:Based on provincial panel data from 2007 to 2023,the medical insurance fund expenditure was employed as a proxy for medical security.Methodologies include Moran's I tests,dual-way fixed effects models,Spatial Durbin Models(SDM)with adjacency and inverse-distance matrices,and spatial mediation models were applied to comprehensively analyze the direct effects,spatial spillover effects,and mediating pathways of regional innovation on medical security.Results:During the observation period,medical security levels in all provinces showed a fluctuating upward trend with significant regional disparities,where eastern provinces outperformed central and western regions.Regional innovation exhibited significant positive direct effects and spatial spillover effects on medical security.Regional innovation can improve medical insurance coverage by promoting growth in medical financial expenditure and the conversion of medical patents.Conclusion:Policy recommendations include deepening the synergy between regional innovation and medical security,optimizing the allocation of medical fiscal resources,accelerating the transformation of technological innovations,and narrowing regional development gaps.