Objective To investigate the effect of polydatin(PD)on myocardial injury in rats with doxorubicin-induced dilated cardiomyopathy(DCM)based on the sonic hedgehog(Shh)/glioma-associated oncogene 1(Gli1)signaling pathway.Methods A total of 60 SD rats were divided into six groups using a randomization method:Con group,DCM group,PD low-dose group,PD medium-dose group,PD high-dose group,and Cyclopamine group,each group contained 10 rats.Except for the Con group,all other groups were induced with doxorubicin to establish DCM rat models.The cardiac function parameters of each group were measured,and pathological changes including myocardial tissue fibrosis and cardiomyocyte apoptosis were observed.Serum inflammatory factors,type Ⅰ and type Ⅲ collagen expression in myocardial tissues,as well as Shh/Gli1 signaling pathway protein expression in myocardial tissues of all groups were analyzed.Results Compared with Con group,rats in DCM group exhibited more severe histopathological damage and fibrosis in myocardial tissues.Significant increases were observed in left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),myocardial cell apoptosis rate,as well as the average absorbance values of interleukin(IL)-1β,IL-18,type Ⅰ collagen,and type Ⅲ collagen(P＜0.05).Left ventricular ejection fraction(LVEF),left ventricular fractional shortening(FS),Shh,and Gli1 expression levels decreased significantly(P＜0.05).Compared with the DCM group,histopathological damage and fibrosis reduced in the PD low-dose,medium-dose,and high-dose groups.LVESD,LVEDD,myocardial cell apoptosis rate decreased,and average absorbance values of IL-1β,IL-18,type Ⅰ collagen,and type Ⅲ collagen reduced.LVEF,FS,Shh,and Gli1 expression levels increased significantly(P＜0.05).Compared with PD high-dose group,pathological damage and fibrosis of myocardial tissue increased,while LVESD,LVEDD,cardiomyocyte apoptosis rate,IL-1β,IL-18,average absorbance of type Ⅰ collagen and type Ⅲcollagen increased,and expression of LVEF,FS,Shh and Gli1 Decreased in cyclopamine group(P＜0.05).Conclusion PD may improve cardiomyopathy in DCM rats by activating Shh/Gli1 signaling pathway.

Objective:To explore the effect, postoperative mucosal pathological changes and molecular biological changes of reboot operation for type 2 inflammation chronic rhinosinusitis with nasal polyps（CRSwNP） patients, and to provide theoretical basis for the clinical application of this kind of operation. Methods:We collected 29 patients who were diagnosed with CRSwNP with type 2 inflammatino response and underwent Reboot surgery from June 2022 to August 2023, and 27 patients who were diagnosed with deviated septum and underwent simple submucosal resection of the septum as the control group. We conducted nasal symptom scoring, endoscopic sinusitis scoring, and CT scanning of the sinuses before and after surgery, as well as HE staining, immunohistochemical staining, and detection of inflammatory factors using Elisa kits at the time of surgery, 1, 3, and 6 months postoperatively. We also observed the ultrastructural changes using transmission electron microscopy and scanning electron microscopy, and performed proteomic analysis of the mucosa in the ethmoid sinus area of the sinusitis patients at the time of surgery and 6 months postoperatively. Results:After 6 months of postoperative follow-up, CT scores of the nasal cavity and sinuses had gradually decreased compared with the preoperative period. The VAS score of main symptoms, SNOT-22 score and Lund-Kennedy endoscopic score were decreased after 12 months follow-up. The histological morphology of the mucosa in the area of the screen was significantly improved compared with the preoperative period, with a reduction in the number of eosinophils. The levels of inflammatory factors such as IL-4 and IL-5 et al. in the mucosa of the area of the screen were gradually reduced compared with the preoperative period. The histological morphology, ultrastructure, and cilia structure of the mucosa in the area of the screen were gradually improved compared with the preoperative period, though not recovered completely. The number of CD4⁺T and CD8⁺T cells not changed significantly before and after the surgery yet. By conducting proteomic analysis of the ethmoidal sinus mucosa before and after surgery, differential proteins were selected, and bioinformatics analysis was conducted on the differentially expressed proteins. By using cytoHubba to identify hub genes and intersecting them with the genes related to chronic sinusitis, we found that MMP9 expression increased in non-type 2 CRS and type 2 CRS in sequence, while ACTC1 expression decreased in non-tpye 2 CRS and type 2 CRS in sequence. Conclusion:Reboot surgery can improve the postoperative symptoms and signs of patients, improve the pathological morphology of the mucosa, and influence the expression of protein after surgery. However, the surgery may not have a significant impact on the distribution of T cell subpopulations and inflammation signal pathway in the nasal mucosa.
Humans ; Sinusitis/metabolism* ; Nasal Polyps/metabolism* ; Nasal Mucosa/ultrastructure* ; Chronic Disease ; Rhinitis/complications* ; Inflammation ; Male ; Female ; Postoperative Period ; Adult ; Interleukin-5/metabolism* ; Interleukin-4/metabolism* ; Middle Aged ; Proteomics ; Rhinosinusitis

Objective To comprehensively assess the binocular function of college students with different glasses-wearing habits and myopic anisometropia using an eye-tracking-based,naked-eye 3D visual inspection system.Methods Between December 2023 and March 2024,a total of 137 myopic(equivalent spherical diopter per eye ≤-0.50 D)students were recruited at Sichuan University.They were divided into 2 groups,including a group of 78 participants who wore glasses continuously,and another group of 59 participants who did not wear glasses continuously.According to the degree of myopic anisometropia(the difference between the equivalent spherical diopters,ΔSE),the 2 groups were further divided into subgroups A(0 D＜ΔSE ≤ 1.00 D)and B(ΔSE＞1.00 D).Binocular function was examined using a naked-eye 3D display device based on human eye tracking,and the correlation between different glasses-wearing habits and myopic anisometropia and binocular visual function was analyzed.Results There was no statistically significant difference in the degree of myopic anisometropia between the continuous glasses-wearing group([1.15±1.15]D)and the non-continuous glasses-wearing group([1.20±1.15]D)(P=0.798).In addition,there was no statistically significant difference in refraction between the continuous glasses-wearing group and the non-continuous glasses-wearing group(P=0.158).There were statistically significant differences in the perceptual eye position of the right eye between the continuous glasses-wearing subgroups A and B(P＜0.05).There were statistically significant differences in coarse stereopsis between the non-continuous glasses-wearing subgroups A and B(P＜0.05).There were statistically significant differences in foveal suppression,fine stereopsis,and perceptual eye position between the continuous glasses-wearing group and the non-continuous glasses-wearing group(P＜0.05).Conclusion Both glasses-wearing habits and myopic anisometropia have effects on the binocular visual function of college students.

Objective:To evaluate the effects of nasal endoscopy in curettage of large mandibular cyst.Methods:20 cases of large mandibular cyst admitted to Xianyang Hosptial of Yan'an University from January 2022 to December 2023 were included.The curet-tage of mandibular cyst was performed under general anesthesia through intraoral incision by nasal endoscopy-assisted illumination and enlargement of the lesion area during the operation.The application effects were evaluated from the aspects of surgical incision length,nerve injury and cyst recurrence.Results:During operation,the surgical filed of view was clear and the operation was suc-ceeded in all cases.There was no complication in and after the nasal endoscopy-assisted curettage,no cyst recurrence was observed during 1-year follow-up.Conclusion:Nasal endoscopy-assisted curettage of large mandibular cyst is effective and safe.

Artificial intelligence (AI) technology enhances the function of acupuncture clinical decision support system (CDSS) by promoting the accuracy of its diagnosis, assisting the formulation of personalized therapeutic regimen, and realizing the scientific and precise evaluation of its therapeutic effect. This paper deeply analyzes the unique advantages of AI-based acupuncture CDSS, including the intelligence and high efficiency. Besides, it points out the challenges of data security, the lack of model interpretation and the complexity of interdisciplinary cooperation in the development of acupuncture CDSS. With the continuous development and improvement of AI technology, acupuncture CDSS is expected to play a more important role in the fields of personalized medicine, telemedicine and disease prevention, and to further advance the efficiency and effect of acupuncture treatment, drive the modernization of acupuncture, and enhance its position and influence in the global healthcare system.
Humans ; Acupuncture Therapy ; Artificial Intelligence ; Decision Support Systems, Clinical

Objective:To evaluate the effects of nasal endoscopy in curettage of large mandibular cyst.Methods:20 cases of large mandibular cyst admitted to Xianyang Hosptial of Yan'an University from January 2022 to December 2023 were included.The curet-tage of mandibular cyst was performed under general anesthesia through intraoral incision by nasal endoscopy-assisted illumination and enlargement of the lesion area during the operation.The application effects were evaluated from the aspects of surgical incision length,nerve injury and cyst recurrence.Results:During operation,the surgical filed of view was clear and the operation was suc-ceeded in all cases.There was no complication in and after the nasal endoscopy-assisted curettage,no cyst recurrence was observed during 1-year follow-up.Conclusion:Nasal endoscopy-assisted curettage of large mandibular cyst is effective and safe.

Objective To investigate the effect of polydatin(PD)on myocardial injury in rats with doxorubicin-induced dilated cardiomyopathy(DCM)based on the sonic hedgehog(Shh)/glioma-associated oncogene 1(Gli1)signaling pathway.Methods A total of 60 SD rats were divided into six groups using a randomization method:Con group,DCM group,PD low-dose group,PD medium-dose group,PD high-dose group,and Cyclopamine group,each group contained 10 rats.Except for the Con group,all other groups were induced with doxorubicin to establish DCM rat models.The cardiac function parameters of each group were measured,and pathological changes including myocardial tissue fibrosis and cardiomyocyte apoptosis were observed.Serum inflammatory factors,type Ⅰ and type Ⅲ collagen expression in myocardial tissues,as well as Shh/Gli1 signaling pathway protein expression in myocardial tissues of all groups were analyzed.Results Compared with Con group,rats in DCM group exhibited more severe histopathological damage and fibrosis in myocardial tissues.Significant increases were observed in left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),myocardial cell apoptosis rate,as well as the average absorbance values of interleukin(IL)-1β,IL-18,type Ⅰ collagen,and type Ⅲ collagen(P＜0.05).Left ventricular ejection fraction(LVEF),left ventricular fractional shortening(FS),Shh,and Gli1 expression levels decreased significantly(P＜0.05).Compared with the DCM group,histopathological damage and fibrosis reduced in the PD low-dose,medium-dose,and high-dose groups.LVESD,LVEDD,myocardial cell apoptosis rate decreased,and average absorbance values of IL-1β,IL-18,type Ⅰ collagen,and type Ⅲ collagen reduced.LVEF,FS,Shh,and Gli1 expression levels increased significantly(P＜0.05).Compared with PD high-dose group,pathological damage and fibrosis of myocardial tissue increased,while LVESD,LVEDD,cardiomyocyte apoptosis rate,IL-1β,IL-18,average absorbance of type Ⅰ collagen and type Ⅲcollagen increased,and expression of LVEF,FS,Shh and Gli1 Decreased in cyclopamine group(P＜0.05).Conclusion PD may improve cardiomyopathy in DCM rats by activating Shh/Gli1 signaling pathway.

Proteomic characterization of plasma is critical for the development of novel pharmacodynamic bio-markers.However,the vast dynamic range renders the profiling of proteomes extremely challenging.Here,we synthesized zeolite NaY and developed a simple and rapid method to achieve comprehensive and deep profiling of the plasma proteome using the plasma protein corona formed on zeolite NaY.Specifically,zeolite NaY and plasma were co-incubated to form plasma protein corona on zeolite NaY(NaY-PPC),followed by conventional protein identification using liquid chromatography-tandem mass spectrometry.NaY was able to significantly enhance the detection of low-abundance plasma proteins,minimizing the"masking"effect caused by high-abundance proteins.The relative abundance of middle-and low-abundance proteins increased substantially from 2.54％to 54.41％,and the top 20 high-abundance proteins decreased from 83.63％to 25.77％.Notably,our method can quantify approxi-mately 4000 plasma proteins with sensitivity up to pg/mL,compared to only about 600 proteins iden-tified from untreated plasma samples.A pilot study based on plasma samples from 30 lung adenocarcinoma patients and 15 healthy subjects demonstrated that our method could successfully distinguish between healthy and disease states.In summary,this work provides an advantageous tool for the exploration of plasma proteomics and its translational applications.

Objective:To explore one-stage total knee arthroplasty (TKA) combined with open reduction and internal fixation (ORIF) for knee osteoarthritis complicated with tibial stress fracture.Methods:The 3 patients were retrospectively analyzed who had been treated for knee osteoarthritis complicated with tibial stress fracture at Department of Orthopedics, Ganzhou District People＇s Hospital from March 2018 to March 2020. They were all female, aged from 54 to 76 years (average, 66 years). There were 2 transverse fractures and one short oblique fracture; all of them had knee varus deformity. The Hospital for Special Surgery (HSS) scores averaged 37.6 (from 28 to 50) for the left knee and 28.3 (from 22 to 39) for the right knee. One-stage TKA was performed for the articular surface while ORIF for the right tibial stress fracture for all patients. Recorded were fracture union time, HSS knee score and range of articular motion.Results:The 3 patients were followed up for 25 to 44 months (average, 32 months).The fracture union time ranged from 4 to 7 months (average, 5 months). The last follow-ups revealed no such complications as prosthesis loosening, peri-prosthesis osteolysis or joint instability. Knee varus deformity was corrected in all patients. The HSS knee scores at the last follow-up averaged 89.6 (from 88 to 91) for the left knee and 88.3 (from 85 to 90) for the right knee.Conclusion:In the treatment of knee osteoarthritis complicated with tibial stress fracture, one-stage TKA combined with ORIF can restore the function of knee joint, leading to fine curative effects.

Advances in high-throughput sequencing (HTS) have fostered rapid developments in the field of microbiome research, and massive microbiome datasets are now being generated. However, the diversity of software tools and the complexity of analysis pipelines make it difficult to access this field. Here, we systematically summarize the advantages and limitations of microbiome methods. Then, we recommend specific pipelines for amplicon and metagenomic analyses, and describe commonly-used software and databases, to help researchers select the appropriate tools. Furthermore, we introduce statistical and visualization methods suitable for microbiome analysis, including alpha- and beta-diversity, taxonomic composition, difference comparisons, correlation, networks, machine learning, evolution, source tracing, and common visualization styles to help researchers make informed choices. Finally, a step-by-step reproducible analysis guide is introduced. We hope this review will allow researchers to carry out data analysis more effectively and to quickly select the appropriate tools in order to efficiently mine the biological significance behind the data.