Objective:To investigate the heterogeneity of peripheral blood neutrophils in sepsis and provide reference for the diagnosis of sepsis.Methods:Twenty-four male C57BL/6 mice were divided into two groups, control and sepsis groups, with 12 mice in each group using a completely randomized design. The mice in the control group were injected with 100 μl saline through the tail vein, while the mice in the sepsis group were injected with an equal amount of Escherichia coli solution (3.33 McFarland turbidity standards) through the tail vein to establish the sepsis model. Peripheral blood samples were collected, and the proportions of neutrophils expressing different surface markers were detected using mass cytometry. GO and KEGG analyses were performed on neutrophil subsets with high CD62L expression, and public datasets were used for verification. The protein-protein interaction networks of CD62L were investigated to assess the value of neutrophil heterogeneity in the diagnosis of sepsis. Results:There were significant differences in the expression of markers in peripheral blood samples between the sepsis group and the control group. CD62L + neutrophil subsets were found in mice with sepsis. GO and KEGG analyses showed that CD62L + neutrophil subsets were associated with multiple biological processes and signaling pathways such as cell adhesion, migration, surface receptor activation, and immune regulation. Clinical results confirmed the correlation of neutrophil CD62L expression with the severity and prognosis of sepsis. The number of subpopulations expressing CD62L in peripheral blood neutrophils in the sepsis group was higher than that in the control group, but the expression level of CD62L in single cells in the sepsis group was significantly lower than that in the control group ( P<0.01). Protein-protein interaction network analysis showed strong interaction between CD62L and multiple important proteins such as P-selectin, P-selectin ligand, E-selectin, and vascular cell adhesion molecule-1. Conclusion:There is heterogeneity in the surface markers of neutrophils between sepsis mice and control mice, which may be a potential indicator for the diagnosis of sepsis.

Objective:To investigate the heterogeneity of peripheral blood neutrophils in sepsis and provide reference for the diagnosis of sepsis.Methods:Twenty-four male C57BL/6 mice were divided into two groups, control and sepsis groups, with 12 mice in each group using a completely randomized design. The mice in the control group were injected with 100 μl saline through the tail vein, while the mice in the sepsis group were injected with an equal amount of Escherichia coli solution (3.33 McFarland turbidity standards) through the tail vein to establish the sepsis model. Peripheral blood samples were collected, and the proportions of neutrophils expressing different surface markers were detected using mass cytometry. GO and KEGG analyses were performed on neutrophil subsets with high CD62L expression, and public datasets were used for verification. The protein-protein interaction networks of CD62L were investigated to assess the value of neutrophil heterogeneity in the diagnosis of sepsis. Results:There were significant differences in the expression of markers in peripheral blood samples between the sepsis group and the control group. CD62L + neutrophil subsets were found in mice with sepsis. GO and KEGG analyses showed that CD62L + neutrophil subsets were associated with multiple biological processes and signaling pathways such as cell adhesion, migration, surface receptor activation, and immune regulation. Clinical results confirmed the correlation of neutrophil CD62L expression with the severity and prognosis of sepsis. The number of subpopulations expressing CD62L in peripheral blood neutrophils in the sepsis group was higher than that in the control group, but the expression level of CD62L in single cells in the sepsis group was significantly lower than that in the control group ( P<0.01). Protein-protein interaction network analysis showed strong interaction between CD62L and multiple important proteins such as P-selectin, P-selectin ligand, E-selectin, and vascular cell adhesion molecule-1. Conclusion:There is heterogeneity in the surface markers of neutrophils between sepsis mice and control mice, which may be a potential indicator for the diagnosis of sepsis.