Inflammatory bowel disease (IBD), a chronic recurrent inflammatory bowel disease, is associated with depression and anxiety. Intestinal inflammation increases susceptibility to psychiatric disorders through a multidimensional network of immune-inflammatory, neuroendocrine, intestinal flora, and intestinal barrier abnormalities. Conversely, psychiatric stress exacerbates the pathology of IBD through immune regulation, intestinal barrier disruption, and microenvironmental alterations. Therefore, it is important to explore the mechanisms of comorbidity between IBD and depression and anxiety to optimise clinical management. This article reviews the comorbid mechanisms of IBD and depression and anxiety from the perspectives of immune-inflammatory axis, intestinal flora-metabolic pathway, intestinal barrier dysfunction, and neuroendocrine regulation in the context of rodent models, and explore potential intervention strategies, with the aim of providing new ideas and targets for therapeutic and transformation research in this field.

Objective To compare the differences in stress on maxillary anterior implants and labial cortical bone under varying thicknesses of palatal bone plates through three-dimensional finite element analysis.Methods Using CBCT scan data and finite ele-ment software,a three-dimensional finite element model of maxillary central incisor implant restoration was constructed.The thickness of the palatal bone plate at the cervical region of the implant was set to 0,0.5,1.0 mm,respectively.The effects of different palatal bone plate thicknesses on the equivalent stress of the implant and the minimum principal stress(compressive stress)of the labial corti-cal bone under normal occlusal conditions were simulated.Results Under normal occlusion,when the palatal cervical bone plate thickness was 0,0.5,1.0 mm,the maximum equivalent stress of the implant was consistently located at the midline of the labial cervi-cal region at the implant-abutment interface,with values of 106.8,103.5,99.71 MPa,respectively.Meanwhile,the minimum princi-pal stress of the cortical bone appeared at the alveolar crest at the junction of the implant-abutment and labial alveolar bone,measuring 107.8,103.2,95.55 MPa,respectively.The results indicated that as the palatal cervical bone plate thickness decreased,both the maximum equivalent stress of the implant and the minimum principal stress of the labial cortical bone exhibited an increasing trend,though they remained below their respective yield strengths.Conclusion From a biomechanical perspective,maxillary anterior implant restoration remains feasible even when the thickness of the palatal bone plate at the cervical region of the implant is 0 mm.

Inflammatory bowel disease (IBD), a chronic recurrent inflammatory bowel disease, is associated with depression and anxiety. Intestinal inflammation increases susceptibility to psychiatric disorders through a multidimensional network of immune-inflammatory, neuroendocrine, intestinal flora, and intestinal barrier abnormalities. Conversely, psychiatric stress exacerbates the pathology of IBD through immune regulation, intestinal barrier disruption, and microenvironmental alterations. Therefore, it is important to explore the mechanisms of comorbidity between IBD and depression and anxiety to optimise clinical management. This article reviews the comorbid mechanisms of IBD and depression and anxiety from the perspectives of immune-inflammatory axis, intestinal flora-metabolic pathway, intestinal barrier dysfunction, and neuroendocrine regulation in the context of rodent models, and explore potential intervention strategies, with the aim of providing new ideas and targets for therapeutic and transformation research in this field.

Objective To compare the differences in stress on maxillary anterior implants and labial cortical bone under varying thicknesses of palatal bone plates through three-dimensional finite element analysis.Methods Using CBCT scan data and finite ele-ment software,a three-dimensional finite element model of maxillary central incisor implant restoration was constructed.The thickness of the palatal bone plate at the cervical region of the implant was set to 0,0.5,1.0 mm,respectively.The effects of different palatal bone plate thicknesses on the equivalent stress of the implant and the minimum principal stress(compressive stress)of the labial corti-cal bone under normal occlusal conditions were simulated.Results Under normal occlusion,when the palatal cervical bone plate thickness was 0,0.5,1.0 mm,the maximum equivalent stress of the implant was consistently located at the midline of the labial cervi-cal region at the implant-abutment interface,with values of 106.8,103.5,99.71 MPa,respectively.Meanwhile,the minimum princi-pal stress of the cortical bone appeared at the alveolar crest at the junction of the implant-abutment and labial alveolar bone,measuring 107.8,103.2,95.55 MPa,respectively.The results indicated that as the palatal cervical bone plate thickness decreased,both the maximum equivalent stress of the implant and the minimum principal stress of the labial cortical bone exhibited an increasing trend,though they remained below their respective yield strengths.Conclusion From a biomechanical perspective,maxillary anterior implant restoration remains feasible even when the thickness of the palatal bone plate at the cervical region of the implant is 0 mm.

【Objective】 To investigate the situation of carbapenem-resistant Enterobacteriaceae(CRE) colonization in patients undergoing haploidentical hematopoietic stem cell transplantation (haplo-HSCT). 【Methods】 A total of 241 consecutive patients who underwent haplo-HSCT in the First Affiliated Hospital of Soochow University from June 1, 2021 to June 1, 2022 were enrolled. Anal swab screening was performed within 48 hours of admission and blood cultures were taken when the patient developed fever. Univariate and multivariate analysis were used to analyze the colonization rate, distribution, risk factors and the correlation between CRE colonization and post-transplant bloodstream infection(BSI). 【Results】 Among 241 patients with haplo-HSCT, there were 90 cases in CRE colonization positive group, with a colonization rate of 37.3% (90/241). Multivariate logistic regression analysis showed that sex (OR 2.42, 95% CI 1.38-4.22, P<0.05) and history of infection within 30 days before transplantation (OR 3.37, 95% CI 1.59-7.17, P<0.05) may be independent risk factors for CRE intestinal colonization. Of the 95 CRE strains, the top five species were carbapenem-resistant Klebsiella pneumoniae (38/95, 40.0%), carbapenem-resistant Escherichia coli (29/95, 30.5%), carbapenem-resistant Enterobacter cloacae (13/95, 13.6%), carbapenem-resistant Klebsiella acidophilus (6/95, 6.3%) and carbapenem-resistant Proteus mirabilis (3/95, 3.1%). The incidence of post-transplant BSI was 12.0% (29/241) in the CRE-colonized group and 3.3% (8/241) in the non-colonized group. In the colonization group, 100% of the pathogens of BSI were identical with those of CRE colonization. 【Conclusion】 Bacterial culture of anal swab during haplo-HSCT is helpful for detection of CRE colonization in intestinal tract, which provides some clinical basis for active monitoring of key flora, prevention and control of infection.

Objective:To investigate the expression of multiple myeloma oncogene 1 (MUM1) in patients with recurrent implantation failure (RIF) and its diagnostic value in chronic endometritis (CE).Methods:The data of 112 patients with RIF treated in the Reproductive Medicine Centre of the Second Affiliated Hospital of Zhengzhou University from October 2019 to October 2021 were collected by cohort study. All patients underwent hysteroscopy, endometrial tissue was collected and the positive expressions of MUM1 and CD138 in endometrial tissue were detected by immunohistochemistry staining; the differences in the positive expression rates of MUM1 and CD138 in endometrium were compared; the consistency of hysteroscopy combined with MUM1 and combined with CD138 immunohistochemistry staining in the diagnosis of CE was compared by Kappa test; the sensitivity of MUM1 and CD138 in diagnosing CE was compared by four-tabular method.Results:There were 55 cases of CE diagnosed by hysteroscopy in 112 patients with RIF, and the incidence was 49.11%. The positive expression rate of MUM1 was higher than that of CD138 [52.68% (59/112) vs. 41.07% (46/112), P<0.001] in endometrium of 112 RIF patients; the Kappa value of hysteroscopy combined with MUM1 and combined with CD138 immunohistochemistry staining in the diagnosis of CE was 0.794; the sensitivity and negative predictive value of endometrial MUM1 immunohistochemistry staining in the diagnosis of CE were higher than those of CD138 [100% (59/59) vs. 77.97% (46/59) and 100% (53/53) vs. 80.31% (53/66), all P<0.001]. Conclusion:In patients with RIF, hysteroscopy combined with endometrial MUM1 immunohistochemistry staining can be used as a more accurate method for diagnosing CE.

Objective:To investigate the expression of multiple myeloma oncogene 1 (MUM1) in patients with recurrent implantation failure (RIF) and its diagnostic value in chronic endometritis (CE).Methods:The data of 112 patients with RIF treated in the Reproductive Medicine Centre of the Second Affiliated Hospital of Zhengzhou University from October 2019 to October 2021 were collected by cohort study. All patients underwent hysteroscopy, endometrial tissue was collected and the positive expressions of MUM1 and CD138 in endometrial tissue were detected by immunohistochemistry staining; the differences in the positive expression rates of MUM1 and CD138 in endometrium were compared; the consistency of hysteroscopy combined with MUM1 and combined with CD138 immunohistochemistry staining in the diagnosis of CE was compared by Kappa test; the sensitivity of MUM1 and CD138 in diagnosing CE was compared by four-tabular method.Results:There were 55 cases of CE diagnosed by hysteroscopy in 112 patients with RIF, and the incidence was 49.11%. The positive expression rate of MUM1 was higher than that of CD138 [52.68% (59/112) vs. 41.07% (46/112), P<0.001] in endometrium of 112 RIF patients; the Kappa value of hysteroscopy combined with MUM1 and combined with CD138 immunohistochemistry staining in the diagnosis of CE was 0.794; the sensitivity and negative predictive value of endometrial MUM1 immunohistochemistry staining in the diagnosis of CE were higher than those of CD138 [100% (59/59) vs. 77.97% (46/59) and 100% (53/53) vs. 80.31% (53/66), all P<0.001]. Conclusion:In patients with RIF, hysteroscopy combined with endometrial MUM1 immunohistochemistry staining can be used as a more accurate method for diagnosing CE.

OBJECTIVE:To prepare Ferulic acid/K/β-CD/metal organic framework (FA/K/β-CD/MOF) inclusion,and to opti-mize its preparation technology. METHODS:K/β-CD/MOF was synthesized by solvothermal method as inclusion material. Using FA as main component,FA/K/β-CD/MOF was prepared by grinding method. The preparation technology was optimized by orthogo-nal test using mole ratio of main component-inclusion material,grinding time,dropping time and inclusion temperature as factors, inclusion rate as index. Prepared FA/K/β-CD/MOF was indentified by IR spectrum and DSC,and inclusion rate and dissolution rate were determined. RESULTS:Optimized preparation technology was as follows as mole ratio of main main component to inclusion material 3∶1,dropping time 60 min,inclusion temperature 40 ℃,inclusion time 60 min. Prepared FA/K/β-CD-MOF had already formed a new kind of phase,and its average inclusion rate was(18.0±1.6)%(RSD=0.9%,n=6);its solubility was 15 times as much as FA(9.582 mg/ml vs. 0.647 mg/ml). CONCLUSIONS:FA/K/β-CD/MOF is prepared successfully;and the preparation tech-nology is reasonable and feasible.

Objective To evaluate early diagnositic value of thyroid transcription factor-1(TTF-1) for patients with lung cancer.Method Ninety-nine lung cancer tissue samples and 21 non-neoplastic lung tissue samples were collected from surgical resections.TTF-1 expressions in the above samples were assessed by micro array combined with immunohisto-chemistry assay.According to percentage of reactive cells,tissue immunoreactivity for TTF-1 were categorized into 3 groups :negative(