This study aims to isolate and identify the Pasteurella multocida(Pm)that caused re-spiratory infections in cattle at a farm in Beijing,and to clarify the biological characteristics of the pathogen,including its serotype,drug resistance,pathogenicity,and genomic features.For this pur-pose,nasopharyngeal swabs and tissue samples were collected from an infected cow for bacterial i-solation and purification,16S rRNA gene amplification,capsular serotyping,virulence gene detec-tion,and whole-genome sequencing of the isolated strain.The results showed that after bacterial i-solation and purification,biochemical tests,and PCR identification,a type A P.multocida strain named"Pm WZ01"was identified.The virulence gene amplification revealed that the Pm WZ01 ge-nome contained the tadD,nanH,ompA,exbB,exbD,and fur genes.Whole-genome sequencing determined that the Pm WZ01 strain had a genome size of 2 383 021 bp with a GC content of 40.28％,containing 56 tRNAs,19 rRNAs,155 pseudogenes,three genomic islands,five prophages,and 55 carbohydrate-active enzyme genes.CARD annotation analysis found one PBP3 resistance gene and two EF-Tu resistance genes.VFDB and PHI-base annotation analysis identified 582 viru-lence factor-related genes and 888 phenotypic mutation genes,etc.Phylogenetic tree analysis revealed that the Pm WZ01 strain is most closely related to the Pm-3 strain(CP014618.1)from Changchun,China,the P1933 strain(CP132898.1)from the UK,and the HB01 strain(CP006976.1)from Hubei,China,belonging to the same clade.This study completed the isolation and identifica-tion of a type A P.multocida strain as well as the analysis of its biological characteristics and whole-genome sequencing,providing a theoretical basis for the prevention and control research of bovine type A P.multocida disease.

This study is to explore the antibiofilm activity of tirapazamine(TPZ)against Salmonella and the mechanism of inhibiting biofilm formation.In this study,crystal violet staining was used to determine the effect of TPZ on the minimal biofilm inhibitory concentration(MBIC)of Salmonel-la and on the development stage of Salmonella biofilm.The effects of TPZ on bacteria and biofilm of Salmonella were observed by scanning electron microscope and fluorescence microscope.Phe-nol-sulfuric acid method,spectrophotometer and BCA method were used to detect the effects of the bacteria on the content of main extracellular matrix of Salmonella.The autocoagulation and hydro-phobicity of Salmonella were measured and their effects on the cell wall of Salmonella were meas-ured by AKP method.The result showed that the MBIC value of TPZ on Salmonella ATCC 14028 was 1.563 μmol/L,it had destructive effects on both Salmonella bacteria and mature biofilm structure,it inhibited the formation of early Salmonella biofilm in a concentration-dependent manner,and inhibited the synthesis and secretion of extracellular polymers in Salmonella biofilm.The autoagglutination ability,hydrophobicity and cell wall integrity of Salmonella were affected.To sum up,the TPZ may inhibit the formation of a large number of Salmonella biofilms in the early stage of biofilm development by affecting the extracellular polymer and cell agglutination a-bility,which is expected to be a potential candidate drug against biofilm infection and provide a new idea for finding alternative antibiotics.

Objective To evaluate the efficiency of intra-tumor and peri-tumor ultrasound radiomics models based on machine learning algorithms for predicting benign and malignant Breast Imaging Reporting and Data System (BI-RADS) 4 breast lesions, and provide insights into early diagnosis of breast cancer. Methods A retrospective analysis was conducted based on the medical records of 450 female patients who underwent breast ultrasound examination in the Affiliated Cancer Hospital of Xinjiang Medical University from June 2020 to April 2022. The patients were divided into the benign (n = 199) and malignant (n = 195) groups according to pathological examination, and randomized into the training (n = 275) and validation (n = 119) sets at a ratio of 7∶3. Radiomics features were extracted and screened. Intra-tumor, peri-tumor, and intra-tumor + peri-tumor ultrasound radiomics models were constructed based on three machine learning algorithms, including logistic regression (LR), support vector machine (SVM), and multi-layer perceptron (MLP). Receiver operating characteristics (ROC) curves, calibration curves, and decision curves were plotted to evaluate the efficacy of the radiomics models for prediction of benign and malignant breast lesions. Results A total of 17 intra-tumor, 16 peri-tumor, and 17 intra-tumor + peri-tumor radiomics features were selected for model construction. Based on LR, MLP, and SVM algorithms, the intra-tumor + peri-tumor radiomics models showed higher predictive efficacy than intra-tumor and peri-tumor radiomics models. The predictive efficacy of intra-tumor, peri-tumor, and intra-tumor + peri-tumor radiomics models were higher based on the SVM algorithm than based on LR and MLP algorithms. For the intra-tumor radiomics model based on the SVM algorithm, the area under the ROC curve (AUC), accuracy, sensitivity, and a specificity were 0.909, 0.851, 0.860, and 0.842, respectively, in the training set and 0.866, 0.832, 0.847, and 0.817, respectively, in the validation set. For the peri-tumor radiomics model based on the SVM algorithm, these values were 0.899, 0.855, 0.882, and 0.827, respectively, in the training set and 0.844, 0.815, 0.847, and 0.783, respectively, in the validation set. For the intra-tumor + peri-tumor radiomics model based on the SVM algorithm, these values were 0.943, 0.876, 0.860, and 0.892, respectively, in the training set and 0.881, 0.849, 0.915, and 0.783, respectively, in the validation set. Conclusion The intra-tumor and peri-tumor ultrasound radiomics models based on machine learning algorithms are highly valuable for prediction of benign and malignant BI-RADS 4 breast lesions. The intra-tumor + peri-tumor ultrasound radiomics model based on the SVM algorithm has the optimal efficacy for prediction of benign and malignant BI-RADS 4 breast lesions.

This study is to explore the antibiofilm activity of tirapazamine(TPZ)against Salmonella and the mechanism of inhibiting biofilm formation.In this study,crystal violet staining was used to determine the effect of TPZ on the minimal biofilm inhibitory concentration(MBIC)of Salmonel-la and on the development stage of Salmonella biofilm.The effects of TPZ on bacteria and biofilm of Salmonella were observed by scanning electron microscope and fluorescence microscope.Phe-nol-sulfuric acid method,spectrophotometer and BCA method were used to detect the effects of the bacteria on the content of main extracellular matrix of Salmonella.The autocoagulation and hydro-phobicity of Salmonella were measured and their effects on the cell wall of Salmonella were meas-ured by AKP method.The result showed that the MBIC value of TPZ on Salmonella ATCC 14028 was 1.563 μmol/L,it had destructive effects on both Salmonella bacteria and mature biofilm structure,it inhibited the formation of early Salmonella biofilm in a concentration-dependent manner,and inhibited the synthesis and secretion of extracellular polymers in Salmonella biofilm.The autoagglutination ability,hydrophobicity and cell wall integrity of Salmonella were affected.To sum up,the TPZ may inhibit the formation of a large number of Salmonella biofilms in the early stage of biofilm development by affecting the extracellular polymer and cell agglutination a-bility,which is expected to be a potential candidate drug against biofilm infection and provide a new idea for finding alternative antibiotics.

This study aims to isolate and identify the Pasteurella multocida(Pm)that caused re-spiratory infections in cattle at a farm in Beijing,and to clarify the biological characteristics of the pathogen,including its serotype,drug resistance,pathogenicity,and genomic features.For this pur-pose,nasopharyngeal swabs and tissue samples were collected from an infected cow for bacterial i-solation and purification,16S rRNA gene amplification,capsular serotyping,virulence gene detec-tion,and whole-genome sequencing of the isolated strain.The results showed that after bacterial i-solation and purification,biochemical tests,and PCR identification,a type A P.multocida strain named"Pm WZ01"was identified.The virulence gene amplification revealed that the Pm WZ01 ge-nome contained the tadD,nanH,ompA,exbB,exbD,and fur genes.Whole-genome sequencing determined that the Pm WZ01 strain had a genome size of 2 383 021 bp with a GC content of 40.28％,containing 56 tRNAs,19 rRNAs,155 pseudogenes,three genomic islands,five prophages,and 55 carbohydrate-active enzyme genes.CARD annotation analysis found one PBP3 resistance gene and two EF-Tu resistance genes.VFDB and PHI-base annotation analysis identified 582 viru-lence factor-related genes and 888 phenotypic mutation genes,etc.Phylogenetic tree analysis revealed that the Pm WZ01 strain is most closely related to the Pm-3 strain(CP014618.1)from Changchun,China,the P1933 strain(CP132898.1)from the UK,and the HB01 strain(CP006976.1)from Hubei,China,belonging to the same clade.This study completed the isolation and identifica-tion of a type A P.multocida strain as well as the analysis of its biological characteristics and whole-genome sequencing,providing a theoretical basis for the prevention and control research of bovine type A P.multocida disease.

Objective:To investigate the risk factors for preoperative hypoxemia in geriatric patients with hip fracture.Methods:A case-control study was used to analyze the clinical data of 99 geriatric patients with hip fracture admitted to Zhongda Hospital affiliated to Southeast University between November 2020 and August 2021. There were 29 males and 70 females, aged 67-96 years [(82.6±6.2)years]. The patients were divided into hypoxemia group ( n=51) and non-hypoxemia group ( n=48) using partial arterial partial pressure of oxygen (PaO 2)<80 mmHg while breathing room air at emergency as the reference standard. The two groups were compared in terms of sex, age, fracture types, body mass index (BMI), American Society of Anesthesiologists (ASA) classification, pulmonary diseases diagnosed by preoperative chest CT [atelectasis, pleural effusion, chronic obstructive pulmonary disease (COPD)], time from injury to visit, New York Heart Association (NYHA) classification, Barthel index, KATZ index, modified Medicine Research Council (mMRC) dyspnea scale, numeric rating scale (NRS), smoking, drinking, comorbidities (hypertension, diabetes mellitus, Parkinson′s disease, Alzheimer′s disease, cerebral infarction, coronary atherosclerotic heart disease), body temperature, blood routine test at first examination (erythrocyte count, leukocyte count, C-reactive protein, hemoglobin), biochemistry (serum albumin, blood glucose, blood creatinine, blood urea nitrogen), electrolyte (serum potassium, serum sodium), and other related examinations [D-dimer, brain natriuretic peptide (BNP), lactic acid]. Univariate analysis was performed to the correlation of those indicators with preoperative hypoxemia. Multivariate Logistic regression analysis was used to identify the independent risk factors for preoperative hypoxemia in geriatric patients with hip fracture. Results:Differences in sex, age, fracture types, BMI, pulmonary diseases diagnosed by preoperative chest CT, time from injury to visit, Barthel index, KATZ index, NRS, smoking, drinking, comorbidities, body temperature, first laboratory results of erythrocyte count, biochemistry, electrolyte and other related examinations were not statistically significant between the two groups (all P>0.05). The two groups showed statistical differences in ASA classification, NYHA classification, mMRC dyspnea scale, leukocyte count at first examination, C-reaction protein and hemoglobin (all P<0.05). Univariate analysis indicated that ASA classification, NYHA classification, mMRC dyspnea scale, leukocyte count at first examination and C-reaction protein were correlated with the occurrence of preoperative hypoxemia in geriatric patients with hip fracture (all P<0.05). Multivariate Logistic regressions analysis indicated that higher mMRC dyspnea scale ( OR=2.30, 95% CI 1.10-4.81, P<0.05), higher leukocyte count at first examination ( OR=1.24, 95% CI 1.05-1.45, P<0.05), higher level of C-reaction protein ( OR=1.02, 95% CI 1.01-1.03, P<0.05) and higher level of hemoglobin ( OR=1.04, 95% CI 1.01-1.07, P<0.05) were significantly correlated with the occurrence of preoperative hypoxemia in geriatric patients with hip fracture. Conclusion:Higher mMRC dyspnea scale, higher leukocyte count, higher level of C-reaction protein and higher level of hemoglobin are independent risk factors for preoperative hypoxemia in geriatric patients with hip fracture.

Objective To explore the clinical efficacy of argon-helium knife combined with pemetrexed in the treatment of patients with advanced non-small cell lung cancer.Methods Totally 80 patients with advanced non-small cell lung cancer (NSCLC) were selected and randomly divided into control group and study group,40 cases in each group.The patients in the control group were treated with argon-helium knife,and the patients in the study group were treated with pemetrexed and argon-helium knife treatment.The adverse reactions,quality of life and clinical efficacy were compared between two groups.Results After treatment,the incidence rate of adverse reactions in the study group was 5％,which was similar with 7.5％ in the control group (P ＞ 0.05).After treatment,the MOS SF-36 increased significantly in both groups,and the study group was significantly higher than the control group (P ＜ 0.05).In the study group,the total effective rate was 92.5％,which was significantly higher than 47.5 ％ in the control group (P ＜ 0.05).Conclusion Argon-helium knife combined with pemetrexed shows a high effective rate in treating patients with advanced non-small cell lung cancer.

Objective To explore the clinical efficacy of argon-helium knife combined with pemetrexed in the treatment of patients with advanced non-small cell lung cancer.Methods Totally 80 patients with advanced non-small cell lung cancer (NSCLC) were selected and randomly divided into control group and study group,40 cases in each group.The patients in the control group were treated with argon-helium knife,and the patients in the study group were treated with pemetrexed and argon-helium knife treatment.The adverse reactions,quality of life and clinical efficacy were compared between two groups.Results After treatment,the incidence rate of adverse reactions in the study group was 5％,which was similar with 7.5％ in the control group (P ＞ 0.05).After treatment,the MOS SF-36 increased significantly in both groups,and the study group was significantly higher than the control group (P ＜ 0.05).In the study group,the total effective rate was 92.5％,which was significantly higher than 47.5 ％ in the control group (P ＜ 0.05).Conclusion Argon-helium knife combined with pemetrexed shows a high effective rate in treating patients with advanced non-small cell lung cancer.

Objective To determine the diagnostic value and necessity of excretory phase of CT urogrphy (CTU). Methods One hundred and one cases with 197 sides of cohort CTU studies were enrolled from PACS system retrospectively, and 44 cases had the records of radiation dosage. Three different seniority senior radiologists reviewed the images and diagnosed 2 times blindly with the clinical information. At the first time, plane scan, contrast enhanced renal cortical phase and renal parenchymal phase images (Diag. A) were reviewed. At the second time, with 3 kinds of images mentioned above plus excretive phase images (Diag. B) were reviewed, with interval of 3 months between the two reviews. With Diag. B as reference standard, accordance rate of diagnoses of Diag. A was calculated. The difference of the 3 rediologists's accordance rates were analyzed with Chi-square test, and the lesions with accordance rate ≤80% were recorded. The cases of 197 urinary tracts were divided into 4 groups: lesions of renal pelvic and ureter, lesions of bladder, lesions of kidney and extra-urinary tract, without lesion. The filling of contrast media in the urinary tract was recorded with 5-scale scoring system. The differences of the urinary tract filling among the 4 groups were analyzed with Kruskal-Wallis test. The effective radiation dosages of CTU with and without excretory phase scan were recorded and calculated, and the difference was analyzed with t test. Result The diagnostic accordance rate of CTU with and without excretory phase of the 3 radiologists was 95.4%(188/197), 93.9%(185/197), 92.4%(182/197)respectively(χ2=1.60, P>0.05). Lesions with diagnostic rate≤80%were parapelvic cyst and caliceal diverticulum. The median score of urinary tracts filling in the lesions of renal pelvic and ureter, lesions of bladder, lesions of kidney and extra-urinary tract, without lesion group were 2, 3, 3, 4, and the differences were significant (HC=31.7, P<0.05). The effective radiation dosages of CTU with and without excretory phase scan were (32.36±11.04)mSv and (41.47±14.27) mSv respectively (t=3.35, P<0.01). Conclusions No significant effect of excretive phase imaging is found on the diagnosis of most urinary system diseases. Poor contrast filling and high rate of failure of filling of the urinary tract with intra luminal pathologies, and increase of the radiation dosage make the excretory phase scan in CTU less useful and it should be used carefully and selectively.

This study was aimed to investigate the research on pharmacological effects, relevant ideas and methods of Guangdong native heat-clearing and toxin-removing herbs. Literatures on pharmacological effects of Guangdong native heat-clearing and toxin-removing herbs were collected, summarized and analyzed. The results showed that most Guangdong native heat-clearing and toxin-removing herbs had obvious effects of anti-microbial, anti-bacterial endotoxin, anti-inflammatory, and antipyretic effect. It also had obvious antivenomous and analgesia effect. Based on research ideas and methods of Guangdong native heat-clearing and toxin-removing herbs, we clarified the confusion species as soon as possible, and adhered to the whole animal experiments in combination with in vitro experiments and computer molecular docking simulations for the complementation of each other. It was concluded that the study on pharmacological effects of Guangdongnative heat-clearing and toxin-removing herbs provided strong support in its clinical application. Ideas and methods provided in this article was the main way to explain pharmacological effects of Guangdong native heat-clearing and toxin-removing herbs.