Objective To analyze the risk factors of intraoperative hypothermia in patients undergoing radical thyroidectomy under laparoscopy and to construct a nomogram prediction model. Methods A total of 336 patients who underwent laparoscopic radical thyroidectomy were selected as study subjects. According to intraoperative body temperature, they were divided into hypothermia group (195 cases) and normal temperature group (141 cases). The risk factors of intraoperative hypothermia in patients undergoing laparoscopic radical thyroidectomy were analyzed using the Logistic regression model. The nomogram prediction model was constructed using R software. The prediction performance of the nomogram prediction model was evaluated using the receiver operating characteristic (ROC) curve, calibration curve, and Hosmer-Lemeshow goodness-of-fit test. Results There were significant differences in age, intraoperative blood loss, amount of fluid infusion, and operation time between the two groups (P < 0.05). Multivariate Logistic regression analysis showed that age>60 years, intraoperative blood loss >60 mL, amount of fluid infusion>1 000 mL, and operation time>2.5 h were risk factors for intraoperative hypothermia in patients undergoing laparoscopic radical thyroidectomy (P < 0.05). A nomogram prediction model was constructed based on the four risk factors. The ROC curve showed that the area under the curve was 0.912 (95%CI, 0.884 to 0.941). The calibration curve and Hosmer-Lemeshow goodness-of-fit test showed that the slope of the calibration curve was close to 1 (χ²=9.140, P=0.243). Conclusion The nomogram prediction model based on age, intraoperative blood loss, amount of fluid infusion, and operation time has good predictive value for intraoperative hypothermia in patients undergoing laparoscopic radical thyroidectomy.

Objectives:To analyze the influence of preoperative neurological dysfunction time on postopera-tive short-term neurological function recovery.Methods:A retrospective analysis was performed on 120 pa-tients with spinal metastases who received surgical treatment from January 2021 to December 2023.There were 68 males and 52 females,aged 58.5±1 1.6 years(27-82 years).The main clinical symptoms were pain in the affected segment and neurological function impairment.The primary tumors were lung cancer(38 cases),kidney cancer(21 cases),breast cancer(13 cases),myeloma(15 cases),lymphoma(5 cases),and other sources of tumor(28 cases).The procedures used included percutaneous vertebroplasty(PVP)(5 cases),total en-bloc spondylectomy(TES)(14 cases),spinal tumor separation surgery(67 cases),and focal palliative excision decom-pression surgery(34 cases).Numerical rating scale(NRS)and American Spinal Injury Association(ASIA)spinal cord injury scale were used to evaluate the pain grade and nerve function of patients before surgery and on the 5th day after surgery,respectively.The degree of spinal cord compression was evaluated using the epidu-ral spinal cord compression(ESCC)scoring system.The time period from muscle strength impairment below the affected plane to surgery for patients with ASIA AB and C was recorded as the time of preoperative neuro-logical dysfunction.For patients with ASIA AB before surgery,three time points of 7d,10d and 15d were selected.For patients with ASIA grade C before surgery,two time points were selected:15d and 30d.The postoperative neurological improvement rate(the proportion of patients with at least one grade improvement of ASIA classification after surgery)of the two groups at different time points were calculated and analyzed.Re-sults:Spinal stability was restored after surgery,and adequate decompression was performed to relieve nerve compression(except PVP).Postoperative NRS scores were significantly lower in all the patients[preoperative 6.00(2.00)vs postoperative 3.00(1.00),P=0.000].The neurological function of some patients was improved im-mediately after operation,and the ASIA grade improved at least one grade in 17 patients(74％)of preopera-tive grades AB,10 patients(63％)of preoperative grade C,and 18 patients(47％)of preoperative grade D.There was no difference in ESCC scores between patients with preoperative grade C and those with preopera-tive grades A and B[3.00(1.00)vs 3.00(0.00),P=0.070].For patients with preoperative ASIA grades AB,the improvement rate of short-term postoperative neurological function in patients with preoperative neurological dysfunction ≤ 10d was significantly better than that in patients with＞10d(＞1 0d 57.1％vs ≤10d 100％,P=0.048).For patients with preoperative ASIA grade C,the improvement rate of short-term postoperative neuro-logical function in patients with preoperative neurological dysfunction ≤30d was significantly better than that in patients with＞30d(＞30d 20.0％vs ≤30d 81.8％,P=0.036).Conclusions:Patients with spinal metastases can get pain relief in the short-term after surgery.The time of neurological dysfunction before surgery has a significant effect on the short-term recovery of neurological function after surgery.

Objectives:To analyze the influence of preoperative neurological dysfunction time on postopera-tive short-term neurological function recovery.Methods:A retrospective analysis was performed on 120 pa-tients with spinal metastases who received surgical treatment from January 2021 to December 2023.There were 68 males and 52 females,aged 58.5±1 1.6 years(27-82 years).The main clinical symptoms were pain in the affected segment and neurological function impairment.The primary tumors were lung cancer(38 cases),kidney cancer(21 cases),breast cancer(13 cases),myeloma(15 cases),lymphoma(5 cases),and other sources of tumor(28 cases).The procedures used included percutaneous vertebroplasty(PVP)(5 cases),total en-bloc spondylectomy(TES)(14 cases),spinal tumor separation surgery(67 cases),and focal palliative excision decom-pression surgery(34 cases).Numerical rating scale(NRS)and American Spinal Injury Association(ASIA)spinal cord injury scale were used to evaluate the pain grade and nerve function of patients before surgery and on the 5th day after surgery,respectively.The degree of spinal cord compression was evaluated using the epidu-ral spinal cord compression(ESCC)scoring system.The time period from muscle strength impairment below the affected plane to surgery for patients with ASIA AB and C was recorded as the time of preoperative neuro-logical dysfunction.For patients with ASIA AB before surgery,three time points of 7d,10d and 15d were selected.For patients with ASIA grade C before surgery,two time points were selected:15d and 30d.The postoperative neurological improvement rate(the proportion of patients with at least one grade improvement of ASIA classification after surgery)of the two groups at different time points were calculated and analyzed.Re-sults:Spinal stability was restored after surgery,and adequate decompression was performed to relieve nerve compression(except PVP).Postoperative NRS scores were significantly lower in all the patients[preoperative 6.00(2.00)vs postoperative 3.00(1.00),P=0.000].The neurological function of some patients was improved im-mediately after operation,and the ASIA grade improved at least one grade in 17 patients(74％)of preopera-tive grades AB,10 patients(63％)of preoperative grade C,and 18 patients(47％)of preoperative grade D.There was no difference in ESCC scores between patients with preoperative grade C and those with preopera-tive grades A and B[3.00(1.00)vs 3.00(0.00),P=0.070].For patients with preoperative ASIA grades AB,the improvement rate of short-term postoperative neurological function in patients with preoperative neurological dysfunction ≤ 10d was significantly better than that in patients with＞10d(＞1 0d 57.1％vs ≤10d 100％,P=0.048).For patients with preoperative ASIA grade C,the improvement rate of short-term postoperative neuro-logical function in patients with preoperative neurological dysfunction ≤30d was significantly better than that in patients with＞30d(＞30d 20.0％vs ≤30d 81.8％,P=0.036).Conclusions:Patients with spinal metastases can get pain relief in the short-term after surgery.The time of neurological dysfunction before surgery has a significant effect on the short-term recovery of neurological function after surgery.

Objective To construct pIRES2-ZsGreen1/FⅨ expression vector,using the pcDNA/ FⅨ plasmid containing FⅨ cDNA as template,and express in HEK-293 cells.Methods The total ORF of F Ⅸ gene was amplified from pcDNA/F Ⅸ plasmid,then the amplified fragment was clonded into the pIRES2-ZsGreen1 vector using the Infusion enzyme.The positive clones of eukaryotic expression vector of pIRES2-ZsGreen1/F Ⅸ were screened and expanded after transfection,then were constructed and confirmed by PCR and sequencing.Transient expression experiments were performed using HEK-293 cells transfected with the expression vectors and observed the expression of ZsGreenl protein by confocal laser microscope.The relative expression levels of F Ⅸ mRNA,protein and F Ⅸ activity (F Ⅸ ∶ C) were detected by real time PCR (RT-PCR),immunofluorescence microscopy,One-Stage method,respectively.Results The expression vector,pIRES2-ZsGreen1/F Ⅸ,was successfully constructed and expressed in HEK-293 cells.RT-PCR detected the expression of F Ⅸ mRNA in HEK-293 cells and the immunofluorescence microscopy showed F Ⅸ protein distributed in the surrounding of nucleus.F Ⅸ ∶ C of cell lysates and cell culture fluid transfected with the expression vectors were (92.03 ± 0.29)％ and (86.89 ± 8.78)％,respectively;while both F Ⅸ ∶ C of cell lysates and cell culture fluid transfected with or without the expression vectors were 0.Conclusion The experimental results showed the expression vector,pIRES2-ZsGreen1/F Ⅸ,was successfully constructed,which provided experiment basement for the follow study on the location,function and molecular pathology of hemophilia B.

Computational Biology ; Factor VII ; Factor VII Deficiency ; Genetic Testing ; Humans ; Pedigree

Adult ; Aged ; Autoimmune Diseases ; genetics ; Case-Control Studies ; Female ; Genetic Predisposition to Disease ; Genotype ; Humans ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Receptors, IgG ; genetics ; Thrombocytopenia ; genetics ; Young Adult