To explore the role of the "Clinical Practice Guidelines" column and others in the Medical Joumnal of Peking Union Medical College Hospital (Med J PUMCH) in promoting diseiplinary development.

Objective:To explore the effect of multidisciplinary comprehensive nursing under the Green Intensive Nursing Model on the clinical outcomes of patients with post-traumatic epilepsy (PTE) .Methods:A convenience sampling method was used to select 120 patients with PTE admitted to the Department of Neurosurgery, Zhumadian Central Hospital, from January 2022 to May 2024. Patients were randomly divided into an intervention group ( n=60) and a control group ( n=60) using a computer-generated random number table. The control group received routine neurosurgical nursing, while the intervention group received multidisciplinary comprehensive nursing under the Green Intensive Nursing Model in addition to routine nursing. The Quality of Life in Epilepsy Inventory-31 (QOLIE-31), Hospital Anxiety and Depression Scale (HADS), Barthel Index, and Montreal Cognitive Assessment (MoCA) were used for evaluation. Seizure frequency, quality of life, anxiety and depression, activities of daily living, and cognitive-behavioral function were compared between the two groups after intervention. Results:At the 3-month follow-up, the intervention group had fewer seizures than the control group, and the difference was statistically significant ( P<0.05). After the intervention, the QOLIE-31 scores, Barthel Index, and MoCA scores of the intervention group were higher than those of the control group, while the HADS scores were lower, the differences were statistically significant ( P<0.05) . Conclusions:Multidisciplinary comprehensive nursing under the Green Intensive Nursing Model can reduce seizure frequency in PTE patients, improve their quality of life, decrease anxiety and depression, enhance daily living ability, and improve cognitive-behavioral function.

Getah virus(GETV)is an arbovirus capable of infecting humans and vertebrates such as horses and pigs via blood-sucking mosquitoes,which is extremely harmful to the livestock indus-try.Current monitoring methods are time-consuming,costly,and dependent on specialized instru-ments.These characteristics hinder rapid detection in clinical samples.Therefore,the development of a simple,rapid,specific,and sensitive method for detecting GETV antigen is crucial for the pre-vention and control of GETV.In this study,a GETV E1 monoclonal antibody strain SD17/09-E1-mAb was prepared by a prokaryotic expression system for GETV E1 protein expression,and a col-loidal gold double-antibody sandwich assay encapsulating two strains of GETV E1 monoclonal an-tibody wasestablished.The results showed that the prepared colloidal gold test strips had good sen-sitivity and did not cross-react with other common porcine virus-positive tissue samples;the test strips had a high compliance rate with the IFA assay for GETV,and could be stored at 37 ℃ for one month and at room temperature for at least three months.In this study,a colloidal gold anti-surveillance test strip for rapid detection of GETV was successfully prepared,which provides a powerful tool for GETV detection.

Objective:To explore the effect of multidisciplinary comprehensive nursing under the Green Intensive Nursing Model on the clinical outcomes of patients with post-traumatic epilepsy (PTE) .Methods:A convenience sampling method was used to select 120 patients with PTE admitted to the Department of Neurosurgery, Zhumadian Central Hospital, from January 2022 to May 2024. Patients were randomly divided into an intervention group ( n=60) and a control group ( n=60) using a computer-generated random number table. The control group received routine neurosurgical nursing, while the intervention group received multidisciplinary comprehensive nursing under the Green Intensive Nursing Model in addition to routine nursing. The Quality of Life in Epilepsy Inventory-31 (QOLIE-31), Hospital Anxiety and Depression Scale (HADS), Barthel Index, and Montreal Cognitive Assessment (MoCA) were used for evaluation. Seizure frequency, quality of life, anxiety and depression, activities of daily living, and cognitive-behavioral function were compared between the two groups after intervention. Results:At the 3-month follow-up, the intervention group had fewer seizures than the control group, and the difference was statistically significant ( P<0.05). After the intervention, the QOLIE-31 scores, Barthel Index, and MoCA scores of the intervention group were higher than those of the control group, while the HADS scores were lower, the differences were statistically significant ( P<0.05) . Conclusions:Multidisciplinary comprehensive nursing under the Green Intensive Nursing Model can reduce seizure frequency in PTE patients, improve their quality of life, decrease anxiety and depression, enhance daily living ability, and improve cognitive-behavioral function.

Objective:To review the literature on improving patients′medical experience, analyze the current research status in this field, and provide references for enhancing patients′medical experience.Methods:A search was conducted for studies related to improving patients′medical experience published from January 2015 to November 2024 in eight databases, including CNKI and Web of Science. Using software CiteSpace 6.4.R1 and VOSviewer 1.6.19 to analyze publication trends, keyword clustering, existing problems in the process of improving patients′medical experience, and the measures taken.Results:6 507 articles were included, of which 4 452 were in English (68.4%) and 2 055 were in Chinese (31.6%). The annual number of publications showed a nearly linear growth trend ( R2 = 0.983 9). The clustering analysis results indicated that domestic and international research mainly focused on patient-centered diagnostic and treatment systems, behaviors, services, and environments. Existing studies revealed that there were still problems such as long waiting times for appointments and consultations (292 articles), uneven distribution of medical resources (198 articles), and poor doctor-patient communication (155 articles). To improve patients′medical experience, the following intervention measures were proposed: accelerating the medical reform process and strengthening government responsibilities (169 articles); enhancing service concepts and innovating service models (363 articles); optimizing the consultation process and simplifying medical procedures (221 articles); improving the consultation environment and optimizing the facilities in the consultation area (181 articles); and integrating technological advantages to cover the entire medical process (346 articles). Conclusions:Research on improving patients' medical experience was increasingly attracting the attention of more and more scholars. Issues such as low service efficiency, unbalanced resource allocation, and poor doctor-patient communication need to be given special attention. In the future, it is recommended to enhance the experience of patients′ medical experience by strengthening policy and institutional guarantees, accelerating the construction of information technology, improving doctor-patient communication skills and humanistic care, and enhancing the capacity of primary medical services.

Getah virus(GETV)is an arbovirus capable of infecting humans and vertebrates such as horses and pigs via blood-sucking mosquitoes,which is extremely harmful to the livestock indus-try.Current monitoring methods are time-consuming,costly,and dependent on specialized instru-ments.These characteristics hinder rapid detection in clinical samples.Therefore,the development of a simple,rapid,specific,and sensitive method for detecting GETV antigen is crucial for the pre-vention and control of GETV.In this study,a GETV E1 monoclonal antibody strain SD17/09-E1-mAb was prepared by a prokaryotic expression system for GETV E1 protein expression,and a col-loidal gold double-antibody sandwich assay encapsulating two strains of GETV E1 monoclonal an-tibody wasestablished.The results showed that the prepared colloidal gold test strips had good sen-sitivity and did not cross-react with other common porcine virus-positive tissue samples;the test strips had a high compliance rate with the IFA assay for GETV,and could be stored at 37 ℃ for one month and at room temperature for at least three months.In this study,a colloidal gold anti-surveillance test strip for rapid detection of GETV was successfully prepared,which provides a powerful tool for GETV detection.

Objective:To explore the application effect of EBN-5A framework nursing intervention in elderly patients with recurrent ischemic stroke after revascularization.Methods:Using the convenience sampling method, 127 elderly patients with recurrent ischemic stroke after revascularization admitted to Zhumadian Central Hospital from April 2021 to April 2023 were selected as the research objects. According to the random number table method, the patients were divided into the experimental group ( n=64) and the control group ( n=63). The control group was given the traditional nursing mode of Neurology, and the experimental group was given the EBN-5A framework nursing intervention plan on the basis of the control group. The improvement rate of clinical symptoms, intracranial blood circulation, and Stroke Impact Scale (SIS) score were compared between the two groups. Results:The clinical improvement rate of the experimental group was higher than that of the control group, the cerebral blood flow and cerebral blood volume were higher than those of the control group, while the mean transit time and time to peak were lower than those of the control group, the SIS score was higher than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The EBN-5A framework nursing intervention plan can improve the clinical symptoms of elderly patients with recurrent ischemic stroke after revascularization, enhance the function of cranial blood vessels, improve the quality of life.

Objective:To explore the application effect of EBN-5A framework nursing intervention in elderly patients with recurrent ischemic stroke after revascularization.Methods:Using the convenience sampling method, 127 elderly patients with recurrent ischemic stroke after revascularization admitted to Zhumadian Central Hospital from April 2021 to April 2023 were selected as the research objects. According to the random number table method, the patients were divided into the experimental group ( n=64) and the control group ( n=63). The control group was given the traditional nursing mode of Neurology, and the experimental group was given the EBN-5A framework nursing intervention plan on the basis of the control group. The improvement rate of clinical symptoms, intracranial blood circulation, and Stroke Impact Scale (SIS) score were compared between the two groups. Results:The clinical improvement rate of the experimental group was higher than that of the control group, the cerebral blood flow and cerebral blood volume were higher than those of the control group, while the mean transit time and time to peak were lower than those of the control group, the SIS score was higher than that of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The EBN-5A framework nursing intervention plan can improve the clinical symptoms of elderly patients with recurrent ischemic stroke after revascularization, enhance the function of cranial blood vessels, improve the quality of life.

Objective:To review the literature on improving patients′medical experience, analyze the current research status in this field, and provide references for enhancing patients′medical experience.Methods:A search was conducted for studies related to improving patients′medical experience published from January 2015 to November 2024 in eight databases, including CNKI and Web of Science. Using software CiteSpace 6.4.R1 and VOSviewer 1.6.19 to analyze publication trends, keyword clustering, existing problems in the process of improving patients′medical experience, and the measures taken.Results:6 507 articles were included, of which 4 452 were in English (68.4%) and 2 055 were in Chinese (31.6%). The annual number of publications showed a nearly linear growth trend ( R2 = 0.983 9). The clustering analysis results indicated that domestic and international research mainly focused on patient-centered diagnostic and treatment systems, behaviors, services, and environments. Existing studies revealed that there were still problems such as long waiting times for appointments and consultations (292 articles), uneven distribution of medical resources (198 articles), and poor doctor-patient communication (155 articles). To improve patients′medical experience, the following intervention measures were proposed: accelerating the medical reform process and strengthening government responsibilities (169 articles); enhancing service concepts and innovating service models (363 articles); optimizing the consultation process and simplifying medical procedures (221 articles); improving the consultation environment and optimizing the facilities in the consultation area (181 articles); and integrating technological advantages to cover the entire medical process (346 articles). Conclusions:Research on improving patients' medical experience was increasingly attracting the attention of more and more scholars. Issues such as low service efficiency, unbalanced resource allocation, and poor doctor-patient communication need to be given special attention. In the future, it is recommended to enhance the experience of patients′ medical experience by strengthening policy and institutional guarantees, accelerating the construction of information technology, improving doctor-patient communication skills and humanistic care, and enhancing the capacity of primary medical services.

Objective To investigate the microbiota composition and diversity between autogenous and anautogenous Culex pipiens pallens, so as to provide insights into unraveling the pathogenesis of autogeny in Cx. pipiens pallens. Methods Autogenous and anautogenous adult Cx. pipiens pallens samples were collected at 25 ℃, and the hypervariable regions of the microbial 16S ribosomal RNA (16S rRNA) gene was sequenced on the Illumina NovaSeq 6000 sequencing platform. The microbiota abundance and diversity were evaluated using the alpha diversity index, and the difference in the microbiota structure was examined using the beta diversity index. The microbiota with significant differences in the abundance between autogenous and anautogenous adult Cx. pipiens pallens samples was identified using the linear discriminant analysis effect size (LEfSe). Results The microbiota in autogenous and anautogenous Cx. pipiens pallens samples belonged to 18 phyla, 28 classes, 70 orders, 113 families, and 170 genera, and the dominant phyla included Proteobacteria, Bacteroidetes, and so on. At the genus level, Wolbachia was a common dominant genus, and the relative abundance was (77.6 ± 11.3)% in autogenous Cx. pipiens pallens samples and (47.5 ± 8.5)% in anautogenous mosquito samples, while Faecalibaculum (0.4% ± 0.1%), Dubosiella (0.5% ± 0.0%) and Massilia (0.5% ± 0.1%) were specific species in autogenous Cx. pipiens pallens samples. Alpha diversity analysis showed that higher Chao1 index and ACE index in autogenous Cx. pipiens pallens samples than in anautogenous samples (both P values > 0.05), and lower Shannon index (P > 0.05) and Simpson index (P < 0.05) in autogenous Cx. pipiens pallens samples than in anautogenous samples. LEfSe analysis showed a total of 48 significantly different taxa between autogenous and anautogenous Cx. pipiens pallens samples (all P values < 0.05). Conclusion There is a significant difference in the microbiota diversity between autogenous and anautogenous Cx. pipiens pallens.