Objective To observe the effects of acupuncture at"Weizhong"on angiogenesis of chronic blunt injury of lumbar muscle in rats;To explore the possible mechanism of acupuncture in the treatment of chronic lumbar skeletal muscle injury based on PI3K/AKT/HIF-1α signaling pathway.Methods A total of 24 SD male rats were established chronic blunt injury of lumbar muscle model,and were randomly divided into model group,Weizhong group and non-acupoint group,with 8 rats in each group.Another 8 rats of the same age were selected as the blank group.In the Weizhong group,acupuncture intervention was performed on both sides of"Weizhong",while in the non-acupoint group,acupuncture intervention was performed on both sides of"Weizhong"inwardly by 0.5 cm,once a day for 20 minutes each time,for 2 consecutive weeks.The blank group and model group adopted the same grasping fixed posture without any intervention.The body mass and limb grip strength of each group were measured,the lumbar muscle tissue morphology was observed by HE staining,the content of NO in lumbar muscle tissue was detected by ELISA,the expression of VEGF in lumbar muscle tissue was observed by immunofluorescence,the expression of PI3K/AKT/HIF-1α pathway protein in lumbar muscle tissue was detected by Western blot.Results Compared with the blank group,body mass and limb grip strength of model group decreased(P<0.01),cell structure of waist skeletal muscle was loosely arranged,red blood cells and nuclear exudation were observed,NO content and positive expression of VEGF in lumbar muscle tissue decreased(P<0.01),and the protein expressions of p-PI3K/PI3K,p-AKT/AKT and HIF-1α in lumbar muscle tissue were down-regulated(P<0.01).Compared with the model group,the body mass and limb grip strength of rats in Weizhong group increased(P<0.05,P<0.01),the arrangement of skeletal muscle cells in the waist was more compact and orderly,and the content of NO and positive expression of VEGF in lumbar muscle tissue were increased(P<0.01),the protein expressions of p-PI3K/PI3K,p-AKT/AKT and HIF-1α in lumbar muscle tissue were up-regulated(P<0.01).The effects of Weizhong group was better than those in non-acupoint group(P<0.05,P<0.01).Conclusion Acupuncture at"Weizhong"can effectively promote the repair of chronic blunt injury of lumbar muscle in rats.The mechanism may be related to activating the PI3K/AKT/HIF-1α pathway to regulate angiogenesis and improve vascular injury.

OBJECTIVE: To explore the effect and mechanism of acupuncture at "Weizhong" (BL40) on microcirculation of paravertebral skeletal muscle in rats with chronic blunt injury of lumbar muscle based on the insulin-like growth factor-1 (IGF-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway. METHODS: Forty-eight SPF-grade SD rats were randomized into a blank group (8 rats) and a modeling group (40 rats). Chronic blunt injury model was established by weight impact method in the modeling group. Forty rats were successfully modeled, and were randomly divided into a model group, an acupuncture at Weizhong group (Weizhong group), an acupuncture at non-acupoint group (non-acupoint group), an inhibitor group, and an inhibitor+acupuncture at Weizhong group (inhibitor+Weizhong group), 8 rats in each group. In the Weizhong group and the inhibitor+Weizhong group, acupuncture was applied at bilateral "Weizhong" (BL40). In the non-acupoint group, acupuncture was applied at non-acupoints, i.e. points 0.5 cm inward from bilateral "Weizhong" (BL40). The acupuncture intervention was delivered 20 min each time, once a day for continuous 2 weeks. In the inhibitor group and the inhibitor+Weizhong group, intraperitoneal injection of IGF-1 receptor (IGF-1R) inhibitor was given once a day, at a dosage of 2 mg/100 g, for continuous 2 weeks. Before modeling and on the 1st, 7th and 14th days of intervention, the body mass was measured. Before and after modeling, and after intervention, the limb grip strength and paw withdrawal threshold (PWT) were measured. After intervention, the morphology of psoas muscle was observed by HE staining; the ultrastructure of psoas muscle capillaries was observed by electron microscopy; the levels of serum vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) were detected by ELISA; and the protein and mRNA expression of IGF-1, IGF-1R, PI3K, AKT of psoas muscle was detected by Western blot and real-time PCR. RESULTS: Compared with the blank group, in the model group, the body mass on the 7th and 14th days of intervention, the limb grip strength, and the PWT of left and right hind feet were decreased (P<0.001, P<0.01); the skeletal muscle cells showed enlarged intercellular space, loosely arranged and irregularly shaped, the capillaries in the psoas muscle tissues were edematous, and the lumen of the blood vessels was obviously atrophied; the levels of serum VEGF and eNOS were decreased (P<0.001); in psoas muscle, the protein expression of IGF-1 and IGF-1R, as well as the p-PI3K/PI3K, p-AKT/AKT values were decreased (P<0.001), the mRNA expression of IGF-1, IGF-1R, PI3K and AKT was decreased (P<0.001, P<0.05). Compared with the model group, in the Weizhong group, the body weight was increased on the 7th and 14th days of intervention (P<0.001), the limb grip strength and the PWT of the left and right hind feet were increased (P<0.001, P<0.01); the arrangement of the skeletal muscle cells was relatively tight and the intercellular space was reduced, the blood vessels tended to be regular and the structure of the basement membrane was continuous, while the lumens of blood vessels were collapsed locally; the levels of serum VEGF and eNOS were increased (P<0.001); in psoas muscle, the protein expression of IGF-1 and IGF-1R, as well as the p-PI3K/PI3K, p-AKT/AKT values were increased (P<0.001), the mRNA expression of IGF-1, IGF-1R, PI3K and AKT was increased (P<0.001, P<0.01). Compared with the model group, in the inhibitor group, the body mass was decreased on the 7th and 14th days of intervention (P<0.05, P<0.01); the limb grip strength and the PWT of the left hind foot were decreased (P<0.01, P<0.001); the intercellular space of skeletal muscle cells was larger, the nuclei of the cells and erythrocytes were scattered in the intercellular space, the damage of the capillaries in the muscular tissues was serious, the collagen fibers were sparsely distributed and disorganized; the levels of serum VEGF and eNOS were decreased (P<0.001, P<0.01); in psoas muscle, the protein expression of IGF-1 and IGF-1R, as well as the p-PI3K/PI3K and p-AKT/AKT values were decreased (P<0.01, P<0.05, P<0.001), the mRNA expression of IGF-1, IGF-1R, PI3K, and AKT was decreased (P<0.01, P<0.001, P<0.05). Compared with the Weizhong group, in the non-acupoint group and the inhibitor+Weizhong group, the body mass was decreased on the 7th and 14th days of intervention (P<0.001, P<0.01), the limb grip strength was decreased (P<0.001); the morphology of muscle cell was relatively poor, with generally irregular, there was mild collapse and atrophy in the vascular lumen, and mild edema in the endothelial cells; the levels of serum VEGF and eNOS were decreased (P<0.001); in psoas muscle, the protein expression of IGF-1 and IGF-1R, as well as the p-PI3K/PI3K and p-AKT/AKT values were decreased (P<0.01, P<0.001), the mRNA expression of IGF-1, IGF-1R, PI3K, and AKT was decreased (P<0.001, P<0.01, P<0.05). Compared with the Weizhong group, the PWT of the left hind foot was decreased in the non-acupoint group (P<0.001), and PWT of the left and right hind feet was decreased in the inhibitor+Weizhong group (P<0.001). CONCLUSION Acupuncture at "Weizhong" (BL40) promotes lumbar muscle repair in chronic low back pain, its mechanism may be related to the activation of the IGF-1/PI3K/AKT pathway, thereby improving the microcirculation.
Animals ; Insulin-Like Growth Factor I/genetics* ; Acupuncture Therapy ; Rats, Sprague-Dawley ; Rats ; Proto-Oncogene Proteins c-akt/genetics* ; Male ; Humans ; Muscle, Skeletal/metabolism* ; Signal Transduction ; Phosphatidylinositol 3-Kinases/genetics* ; Wounds, Nonpenetrating/metabolism* ; Acupuncture Points

Objective To observe the effects of acupuncture at"Weizhong"on angiogenesis of chronic blunt injury of lumbar muscle in rats;To explore the possible mechanism of acupuncture in the treatment of chronic lumbar skeletal muscle injury based on PI3K/AKT/HIF-1α signaling pathway.Methods A total of 24 SD male rats were established chronic blunt injury of lumbar muscle model,and were randomly divided into model group,Weizhong group and non-acupoint group,with 8 rats in each group.Another 8 rats of the same age were selected as the blank group.In the Weizhong group,acupuncture intervention was performed on both sides of"Weizhong",while in the non-acupoint group,acupuncture intervention was performed on both sides of"Weizhong"inwardly by 0.5 cm,once a day for 20 minutes each time,for 2 consecutive weeks.The blank group and model group adopted the same grasping fixed posture without any intervention.The body mass and limb grip strength of each group were measured,the lumbar muscle tissue morphology was observed by HE staining,the content of NO in lumbar muscle tissue was detected by ELISA,the expression of VEGF in lumbar muscle tissue was observed by immunofluorescence,the expression of PI3K/AKT/HIF-1α pathway protein in lumbar muscle tissue was detected by Western blot.Results Compared with the blank group,body mass and limb grip strength of model group decreased(P<0.01),cell structure of waist skeletal muscle was loosely arranged,red blood cells and nuclear exudation were observed,NO content and positive expression of VEGF in lumbar muscle tissue decreased(P<0.01),and the protein expressions of p-PI3K/PI3K,p-AKT/AKT and HIF-1α in lumbar muscle tissue were down-regulated(P<0.01).Compared with the model group,the body mass and limb grip strength of rats in Weizhong group increased(P<0.05,P<0.01),the arrangement of skeletal muscle cells in the waist was more compact and orderly,and the content of NO and positive expression of VEGF in lumbar muscle tissue were increased(P<0.01),the protein expressions of p-PI3K/PI3K,p-AKT/AKT and HIF-1α in lumbar muscle tissue were up-regulated(P<0.01).The effects of Weizhong group was better than those in non-acupoint group(P<0.05,P<0.01).Conclusion Acupuncture at"Weizhong"can effectively promote the repair of chronic blunt injury of lumbar muscle in rats.The mechanism may be related to activating the PI3K/AKT/HIF-1α pathway to regulate angiogenesis and improve vascular injury.

Objective:To correlate serum homocysteine (Hcy) level with psychiatric symptoms and social ability in patients with schizophrenia.Methods:A total of 143 patients with schizophrenia who received treatment in the Third Hospital of Quzhou from January 2018 to January 2020 were included in this study. They were randomly divided into abnormal Hcy group (Hcy ≥ 10) μmol/L, n = 46) and normal Hcy group (Hcy < 10 μmol/L, n = 97) according to different serum Hcy levels. Baseline data, the scores of the Brief Psychiatric Rating Scale (BPRS), the Positive and Negative Syndrome Scale (PANSS), and the Substance Dependence Severity Scale (SDSS) were compared between the two groups. The correlation between serum Hcy level and psychiatric symptoms and social ability was analyzed using the Pearson correlation analysis. Results:PANSS total score and BPRS total score were (77.86 ± 8.37) points and (94.47 ± 9.36) points, respectively in the abnormal Hcy group, and (74.37 ± 7.94) points and (90.35 ± 9.26) points, respectively in the normal Hcy group. There were significant differences in PANSS total score and BPRS total score between the two groups ( t = 2.41, 2.47, both P < 0.05). SDSS total score was significantly higher in the abnormal Hcy group than in the normal Hcy group [(11.75 ± 2.38) points vs. (10.53 ± 2.28) points, t = 2.88, P < 0.05]. Pearson correlation analysis showed that serum Hcy level was positively correlated with PANSS positive subscale score ( r = 0.73, P < 0.001), general mental symptom score ( r = 0.43, P = 0.032) and PANSS total score ( r = 0.53, P = 0.027), but it was not correlated with PANSS negative symptom score ( P > 0.05). Serum Hcy level was positively correlated with BPRS excitement subscale score ( r = 0.42, P = 0.037) and hostility subscale score ( r = 0.37, P = 0.047), but it was not correlated with anxiety, blunted affect, unusual thought content subscale scores and BPRS total score (all P > 0.05). Conclusion:Serum Hcy level is correlated with psychiatric symptoms and social ability in patients with schizophrenia. The higher the level of Hcy, the more severe the psychiatric symptoms, and the higher the degree of social disability.

Objective To understand the epidemic characteristics of different virus types of hand, foot and mouth disease (HFMD) in Zhaotong City, and provide guidance and recommendations for the prevention and control of HFMD, and to analyze seasonal characteristics of different virus types of HFMD in Zhaotong City. Methods The epidemiological characteristics of different virus types of HFMD in Zhaotong City from 2014 to 2017 were analyzed using the concentration and circular distribution methods. Results The main pathogens detected were EV71, Cox A16 and other enteroviruses, which were 216, 182, and 294, respectively, accounting for 57.45%, 73.44%, and 67.11%. M was 0.86, indicating that EV71 had strong seasonality. The Rayleigh test showed statistically significant differences (Z = 99.53, P <0.001). ā = 157 °, the peak day of onset was May 10, similar to untyped (May 16), the peak period was April 21-June 1, and the epidemic period was April 1-June 21. Conclusion According to the incubation period of hand-foot-mouth disease and the period of time during which the vaccine develops protective effects, vaccination of hand-foot-mouth disease vaccine at the peak period has a good guiding significance for the timeliness and pertinence of vaccination.

Objective:To compare two flexible embryo catheters and determine whether clinical out-come differs in the in vitro fertilization-embryo transfer (IVF-ET)cycles.Methods:This prospective control study was conducted by one doctor between July 2012 and November 2013.In the study,2 064 patients undergoing fresh embryo transfer by using IVF-ET/intracytoplasmic sperm injection (ICSI)-ET in Reproductive Medical Center of Peking University Third Hospital were recruited.The subjects were di-vided into two groups.Cook Sydney IVF embryo transfer catheters (product model:K-JETS-7019-SIVF) were used for embryo transfer in group 1 (n =949),and FrydmanCCD catheters (product model:131230301)were used in group 2 (n =1 115).Pregnancy outcomes were compared between these two groups.Results:There was no significant difference in age,diagnosis for infertility and stimulation proto-col used between the two groups.In addition,there was no difference in the number of oocytes collected and in the number and score of embryos transferred.The significantly higher implantation rate,clinical pregnancy rate,and live birth rate (34.40% vs.26.92%,51.21% vs.41.52%,42.57% vs. 33.09%,P <0.05)were observed in group 1 compared with group 2.The abortion rate was not signifi-cantly different between the two groups (11.93% vs.15.98%,P >0.05).The proportion of difficult transfer was higher in group 1 than that in group 2 (5.27% vs.3.41%,P <0.05 ).There was no difference in the clinical pregnancy rate and live birth rate between the two difficult transfer cycles.Con-clusion:The type of embryo transfer catheter affects the clinical outcome in IVF.Good clinical outcome can be obtained by using Cook Sydney IVF catheter,which is worthy of clinical promotion.